Restriction map for
pF1KE0596
(Circular) (Six-base) MAPSORT of: pF1KE0596.seq Check: 2282 from: 1 to: 3581
pF1KE0596 3581 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 619 619
Size: 3581
Acc65I G'GTAC_C
Cuts at: 597 597
Size: 3581
AclI AA'CG_TT
Cuts at: 3421 3421
Size: 3581
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1406 1838 2585 2853 1406
Size: 432 747 268 2134
Fragments arranged by size:
2134 747 432 268
AflIII A'CryG_T
Cuts at: 933 2037 3014 933
Size: 1104 977 1500
Fragments arranged by size:
1500 1104 977
AgeI A'CCGG_T
Cuts at: 1969 1969
Size: 3581
AleI CACnn'nnGTG
Cuts at: 499 499
Size: 3581
AlwNI CAG_nnn'CTG
Cuts at: 537 886 2453 537
Size: 349 1567 1665
Fragments arranged by size:
1665 1567 349
ApaI G_GGCC'C
Cuts at: 178 473 178
Size: 295 3286
ApaLI G'TGCA_C
Cuts at: 2351 2351
Size: 3581
ApoI r'AATT_y
Cuts at: 585 2934 3190 585
Size: 2349 256 976
Fragments arranged by size:
2349 976 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3581
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3581
AvaI C'yCGr_G
Cuts at: 601 601
Size: 3581
BamHI G'GATC_C
Cuts at: 606 606
Size: 3581
BanI G'GyrC_C
Cuts at: 256 406 597 1011 1259 1294 256
Size: 150 191 414 248 35 2543
Fragments arranged by size:
2543 414 248 191 150 35
BanII G_rGCy'C
Cuts at: 136 178 291 462 473 595 1625 136
Size: 42 113 171 11 122 1030 2092
Fragments arranged by size:
2092 1030 171 122 113 42 11
BbeI G_GCGC'C
Cuts at: 1015 1263 1015
Size: 248 3333
BciVI GTATCCnnnnn_n'
Cuts at: 1479 2240 3581 1479
Size: 761 1341 1479
Fragments arranged by size:
1479 1341 761
BclI T'GATC_A
Cuts at: 1101 1101
Size: 3581
BglII A'GATC_T
Cuts at: 1096 3133 1096
Size: 2037 1544
BlpI GC'TnA_GC
Cuts at: 693 693
Size: 3581
Bme1580I G_kGCm'C
Cuts at: 178 259 411 473 1206 1299 2355 178
Size: 81 152 62 733 93 1056 1404
Fragments arranged by size:
1404 1056 733 152 93 81 62
BmgBI CAC'GTC
Cuts at: 501 501
Size: 3581
BmrI ACTGGGnnnn_n'
Cuts at: 793 874 1209 3353 793
Size: 81 335 2144 1021
Fragments arranged by size:
2144 1021 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1948 1948
Size: 3581
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 260
Size: 3581
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 757 1636 2128 2426 757
Size: 879 492 298 1912
Fragments arranged by size:
1912 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3285 3285
Size: 3581
BsaAI yAC'GTr
Cuts at: 934 934
Size: 3581
BsaBI GATnn'nnATC
Cuts at: 6 1095 3132 6
Size: 1089 2037 455
Fragments arranged by size:
2037 1089 455
BsaHI Gr'CG_yC
Cuts at: 1012 1260 1962 3458 1012
Size: 248 702 1496 1135
Fragments arranged by size:
1496 1135 702 248
BsaWI w'CCGG_w
Cuts at: 770 1291 1969 2243 2390 2801 770
Size: 521 678 274 147 411 1550
Fragments arranged by size:
1550 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 376 376
Size: 3581
BseYI C'CCAG_C
Cuts at: 129 159 170 252 416 542 1006 1764 2341
Size: 30 11 82 164 126 464 758 577
Cuts at: 2341 2837 129
Size: 496 873
Fragments arranged by size:
873 758 577 496 464 164 126 82
30 11
BsiEI CG_ry'CG
Cuts at: 104 1123 1169 2377 104
Size: 1019 46 1208 1308
Fragments arranged by size:
1308 1208 1019 46
BsiHKAI G_wGCw'C
Cuts at: 595 1373 1563 2355 595
Size: 778 190 792 1821
Fragments arranged by size:
1821 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 136 178 259 291 411 462 473 595 1206
Size: 42 81 32 120 51 11 122 611
Cuts at: 1206 1299 1373 1563 1625 2355 136
Size: 93 74 190 62 730 1362
Fragments arranged by size:
1362 730 611 190 122 120 93 81
74 62 51 42 32 11
BspEI T'CCGG_A
Cuts at: 2801 2801
Size: 3581
BspHI T'CATG_A
Cuts at: 2757 2757
Size: 3581
BspMI ACCTGCnnnn'nnnn_
Cuts at: 631 845 1147 1528 631
Size: 214 302 381 2684
Fragments arranged by size:
2684 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1873 1927 3411 3576 50
Size: 1823 54 1484 165 55
Fragments arranged by size:
1823 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1493 1493
Size: 3581
BsrFI r'CCGG_y
Cuts at: 388 479 1579 1760 1969 388
Size: 91 1100 181 209 2000
Fragments arranged by size:
2000 1100 209 181 91
BssSI C'ACGA_G
Cuts at: 1852 2210 1852
Size: 358 3223
BstAPI GCAn_nnn'nTGC
Cuts at: 537 958 537
Size: 421 3160
BstBI TT'CG_AA
Cuts at: 1942 1942
Size: 3581
BstYI r'GATC_y
Cuts at: 1 606 1073 1096 1431 1677 2689 2775 2787
Size: 605 467 23 335 246 1012 86 12
Cuts at: 2787 3133 1
Size: 346 449
Fragments arranged by size:
1012 605 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 186 197 329 402 462 107
Size: 79 11 132 73 60 3226
Fragments arranged by size:
3226 132 79 73 60 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1531 1712 1875 1531
Size: 181 163 3237
Fragments arranged by size:
3237 181 163
BtsI GCAGTG_nn'
Cuts at: 824 824
Size: 3581
DraI TTT'AAA
Cuts at: 580 580
Size: 3581
DrdI GACnn_nn'nnGTC
Cuts at: 1287 2145 1287
Size: 858 2723
EaeI y'GGCC_r
Cuts at: 110 200 1166 1340 1731 1758 3103 110
Size: 90 966 174 391 27 1345 588
Fragments arranged by size:
1345 966 588 391 174 90 27
EagI C'GGCC_G
Cuts at: 1166 1166
Size: 3581
EarI CTCTTCn'nnn_
Cuts at: 1604 1814 1604
Size: 210 3371
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1789 2099 2245 3391 1789
Size: 310 146 1146 1979
Fragments arranged by size:
1979 1146 310 146
EcoICRI GAG'CTC
Cuts at: 593 593
Size: 3581
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 1406 1838 2585 2853 260
Size: 1146 432 747 268 988
Fragments arranged by size:
1146 988 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 174 175 245 470 720 174
Size: 1 70 225 250 3035
Fragments arranged by size:
3035 250 225 70 1
EcoRI G'AATT_C
Cuts at: 585 585
Size: 3581
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 390 1600 390
Size: 1210 2371
FspI TGC'GCA
Cuts at: 1362 1362
Size: 3581
HaeII r_GCGC'y
Cuts at: 433 1015 1263 2285 3264 433
Size: 582 248 1022 979 750
Fragments arranged by size:
1022 979 750 582 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 202 234 1423 1453 1455 1485 202
Size: 32 1189 30 2 30 2298
Fragments arranged by size:
2298 1189 32 30 30 2
HincII GTy'rAC
Cuts at: 620 620
Size: 3581
KasI G'GCGC_C
Cuts at: 1011 1259 1011
Size: 248 3333
KpnI G_GTAC'C
Cuts at: 601 601
Size: 3581
MluI A'CGCG_T
Cuts at: 3014 3014
Size: 3581
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2252 2436 2252
Size: 184 3397
MscI TGG'CCA
Cuts at: 1342 1342
Size: 3581
MslI CAynn'nnrTG
Cuts at: 499 1570 1697 499
Size: 1071 127 2383
Fragments arranged by size:
2383 1071 127
MspA1I CmG'CkG
Cuts at: 159 309 395 527 534 692 1006 1366 2379
Size: 150 86 132 7 158 314 360 1013
Cuts at: 2379 2624 159
Size: 245 1116
Fragments arranged by size:
1116 1013 360 314 245 158 150 132
86 7
NaeI GCC'GGC
Cuts at: 390 481 1762 390
Size: 91 1281 2209
Fragments arranged by size:
2209 1281 91
NarI GG'CG_CC
Cuts at: 1012 1260 1012
Size: 248 3333
NcoI C'CATG_G
Cuts at: 107 107
Size: 3581
NgoMIV G'CCGG_C
Cuts at: 388 479 1760 388
Size: 91 1281 2209
Fragments arranged by size:
2209 1281 91
NspI r_CATG'y
Cuts at: 634 1134 2041 634
Size: 500 907 2174
Fragments arranged by size:
2174 907 500
PciI A'CATG_T
Cuts at: 2037 2037
Size: 3581
PmeI GTTT'AAAC
Cuts at: 580 580
Size: 3581
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2720 2752 2720
Size: 32 3549
PpuMI rG'GwC_Cy
Cuts at: 245 245
Size: 3581
PspOMI G'GGCC_C
Cuts at: 174 469 174
Size: 295 3286
PstI C_TGCA'G
Cuts at: 158 446 628 1313 158
Size: 288 182 685 2426
Fragments arranged by size:
2426 685 288 182
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3581
PvuII CAG'CTG
Cuts at: 159 309 395 1006 1366 159
Size: 150 86 611 360 2374
Fragments arranged by size:
2374 611 360 150 86
RsrII CG'GwC_CG
Cuts at: 1776 1776
Size: 3581
SacI G_AGCT'C
Cuts at: 595 595
Size: 3581
SalI G'TCGA_C
Cuts at: 618 618
Size: 3581
SapI GCTCTTCn'nnn_
Cuts at: 1604 1814 1604
Size: 210 3371
SbfI CC_TGCA'GG
Cuts at: 628 628
Size: 3581
ScaI AGT'ACT
Cuts at: 3098 3098
Size: 3581
SfcI C'TryA_G
Cuts at: 33 154 442 624 1309 2302 2493 33
Size: 121 288 182 685 993 191 1121
Fragments arranged by size:
1121 993 685 288 191 182 121
SfoI GGC'GCC
Cuts at: 1013 1261 1013
Size: 248 3333
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3581
SmaI CCC'GGG
Cuts at: 603 603
Size: 3581
SmlI C'TyrA_G
Cuts at: 736 1651 2143 2405 736
Size: 915 492 262 1912
Fragments arranged by size:
1912 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3581
SphI G_CATG'C
Cuts at: 634 1134 634
Size: 500 3081
StuI AGG'CCT
Cuts at: 344 344
Size: 3581
StyI C'CwwG_G
Cuts at: 107 715 1069 107
Size: 608 354 2619
Fragments arranged by size:
2619 608 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 274 430 1164 1297 1756 1965 274
Size: 156 734 133 459 209 1890
Fragments arranged by size:
1890 734 459 209 156 133
TatI w'GTAC_w
Cuts at: 3096 3096
Size: 3581
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3098 3130 3098
Size: 32 3549
Tth111I GACn'n_nGTC
Cuts at: 324 1378 324
Size: 1054 2527
XbaI T'CTAG_A
Cuts at: 612 612
Size: 3581
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 308 1076 308
Size: 768 2813
XmaI C'CCGG_G
Cuts at: 601 601
Size: 3581
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmtI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI