Restriction map for
pF1KE0594
(Circular) (Six-base) MAPSORT of: pF1KE0594.seq Check: 3969 from: 1 to: 3623
pF1KE0594 3623 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 661 661
Size: 3623
Acc65I G'GTAC_C
Cuts at: 639 639
Size: 3623
AclI AA'CG_TT
Cuts at: 3463 3463
Size: 3623
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1448 1880 2627 2895 1448
Size: 432 747 268 2176
Fragments arranged by size:
2176 747 432 268
AflIII A'CryG_T
Cuts at: 975 2079 3056 975
Size: 1104 977 1542
Fragments arranged by size:
1542 1104 977
AgeI A'CCGG_T
Cuts at: 2011 2011
Size: 3623
AhdI GACnn_n'nnGTC
Cuts at: 191 191
Size: 3623
AlwNI CAG_nnn'CTG
Cuts at: 554 928 2495 554
Size: 374 1567 1682
Fragments arranged by size:
1682 1567 374
ApaLI G'TGCA_C
Cuts at: 2393 2393
Size: 3623
ApoI r'AATT_y
Cuts at: 627 2976 3232 627
Size: 2349 256 1018
Fragments arranged by size:
2349 1018 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3623
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3623
AvaI C'yCGr_G
Cuts at: 142 189 237 364 643 142
Size: 47 48 127 279 3122
Fragments arranged by size:
3122 279 127 48 47
BamHI G'GATC_C
Cuts at: 415 648 415
Size: 233 3390
BanI G'GyrC_C
Cuts at: 573 639 1053 1301 1336 573
Size: 66 414 248 35 2860
Fragments arranged by size:
2860 414 248 66 35
BanII G_rGCy'C
Cuts at: 290 637 1667 290
Size: 347 1030 2246
Fragments arranged by size:
2246 1030 347
BbeI G_GCGC'C
Cuts at: 577 1057 1305 577
Size: 480 248 2895
Fragments arranged by size:
2895 480 248
BciVI GTATCCnnnnn_n'
Cuts at: 1521 2282 3623 1521
Size: 761 1341 1521
Fragments arranged by size:
1521 1341 761
BclI T'GATC_A
Cuts at: 1143 1143
Size: 3623
BglII A'GATC_T
Cuts at: 1138 3175 1138
Size: 2037 1586
BlpI GC'TnA_GC
Cuts at: 735 735
Size: 3623
Bme1580I G_kGCm'C
Cuts at: 1248 1341 2397 1248
Size: 93 1056 2474
Fragments arranged by size:
2474 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 564 835 916 1251 3395 564
Size: 271 81 335 2144 792
Fragments arranged by size:
2144 792 335 271 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1990 1990
Size: 3623
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 799 1678 2170 2468 799
Size: 879 492 298 1954
Fragments arranged by size:
1954 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3327 3327
Size: 3623
BsaAI yAC'GTr
Cuts at: 976 976
Size: 3623
BsaBI GATnn'nnATC
Cuts at: 6 1137 3174 6
Size: 1131 2037 455
Fragments arranged by size:
2037 1131 455
BsaHI Gr'CG_yC
Cuts at: 574 1054 1302 2004 3500 574
Size: 480 248 702 1496 697
Fragments arranged by size:
1496 702 697 480 248
BsaWI w'CCGG_w
Cuts at: 282 412 593 812 1333 2011 2285 2432 2843
Size: 130 181 219 521 678 274 147 411
Cuts at: 2843 282
Size: 1062
Fragments arranged by size:
1062 678 521 411 274 219 181 147
130
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 424 424
Size: 3623
BseYI C'CCAG_C
Cuts at: 1048 1806 2383 2879 1048
Size: 758 577 496 1792
Fragments arranged by size:
1792 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1165 1211 2419 104
Size: 1061 46 1208 1308
Fragments arranged by size:
1308 1208 1061 46
BsiHKAI G_wGCw'C
Cuts at: 637 1415 1605 2397 637
Size: 778 190 792 1863
Fragments arranged by size:
1863 792 778 190
BsmI GAATG_Cn'
Cuts at: 467 467
Size: 3623
BsmBI CGTCTCn'nnnn_
Cuts at: 363 490 363
Size: 127 3496
Bsp1286I G_dGCh'C
Cuts at: 290 637 1248 1341 1415 1605 1667 2397 290
Size: 347 611 93 74 190 62 730 1516
Fragments arranged by size:
1516 730 611 347 190 93 74 62
BspEI T'CCGG_A
Cuts at: 282 2843 282
Size: 2561 1062
BspHI T'CATG_A
Cuts at: 2799 2799
Size: 3623
BspMI ACCTGCnnnn'nnnn_
Cuts at: 673 887 1189 1570 673
Size: 214 302 381 2726
Fragments arranged by size:
2726 381 302 214
BsrBI CCG'CTC
Cuts at: 50 225 1915 1969 3453 3618 50
Size: 175 1690 54 1484 165 55
Fragments arranged by size:
1690 1484 175 165 55 54
BsrDI GCAATG_nn'
Cuts at: 242 606 1535 242
Size: 364 929 2330
Fragments arranged by size:
2330 929 364
BsrFI r'CCGG_y
Cuts at: 341 1621 1802 2011 341
Size: 1280 181 209 1953
Fragments arranged by size:
1953 1280 209 181
BssSI C'ACGA_G
Cuts at: 1894 2252 1894
Size: 358 3265
BstAPI GCAn_nnn'nTGC
Cuts at: 596 1000 596
Size: 404 3219
BstBI TT'CG_AA
Cuts at: 1984 1984
Size: 3623
BstYI r'GATC_y
Cuts at: 1 415 648 1115 1138 1473 1719 2731 2817
Size: 414 233 467 23 335 246 1012 86
Cuts at: 2817 2829 3175 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 414 346 335 246 233
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3623
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 215 1573 1754 1917 215
Size: 1358 181 163 1921
Fragments arranged by size:
1921 1358 181 163
BtsI GCAGTG_nn'
Cuts at: 866 866
Size: 3623
DraI TTT'AAA
Cuts at: 622 622
Size: 3623
DrdI GACnn_nn'nnGTC
Cuts at: 1329 2187 1329
Size: 858 2765
EaeI y'GGCC_r
Cuts at: 488 1208 1382 1773 1800 3145 488
Size: 720 174 391 27 1345 966
Fragments arranged by size:
1345 966 720 391 174 27
EagI C'GGCC_G
Cuts at: 1208 1208
Size: 3623
EarI CTCTTCn'nnn_
Cuts at: 476 537 1646 1856 476
Size: 61 1109 210 2243
Fragments arranged by size:
2243 1109 210 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1831 2141 2287 3433 1831
Size: 310 146 1146 2021
Fragments arranged by size:
2021 1146 310 146
EcoICRI GAG'CTC
Cuts at: 635 635
Size: 3623
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1448 1880 2627 2895 1448
Size: 432 747 268 2176
Fragments arranged by size:
2176 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 142 142
Size: 3623
EcoO109I rG'GnC_Cy
Cuts at: 762 762
Size: 3623
EcoRI G'AATT_C
Cuts at: 627 627
Size: 3623
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 474 1642 474
Size: 1168 2455
FspI TGC'GCA
Cuts at: 1404 1404
Size: 3623
HaeII r_GCGC'y
Cuts at: 577 1057 1305 2327 3306 577
Size: 480 248 1022 979 894
Fragments arranged by size:
1022 979 894 480 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 177 209 1465 1495 1497 1527 177
Size: 32 1256 30 2 30 2273
Fragments arranged by size:
2273 1256 32 30 30 2
HincII GTy'rAC
Cuts at: 662 662
Size: 3623
KasI G'GCGC_C
Cuts at: 573 1053 1301 573
Size: 480 248 2895
Fragments arranged by size:
2895 480 248
KpnI G_GTAC'C
Cuts at: 643 643
Size: 3623
MfeI C'AATT_G
Cuts at: 611 611
Size: 3623
MluI A'CGCG_T
Cuts at: 3056 3056
Size: 3623
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2294 2478 2294
Size: 184 3439
MscI TGG'CCA
Cuts at: 490 1384 490
Size: 894 2729
MslI CAynn'nnrTG
Cuts at: 1612 1739 1612
Size: 127 3496
MspA1I CmG'CkG
Cuts at: 261 734 1048 1408 2421 2666 261
Size: 473 314 360 1013 245 1218
Fragments arranged by size:
1218 1013 473 360 314 245
NaeI GCC'GGC
Cuts at: 1804 1804
Size: 3623
NarI GG'CG_CC
Cuts at: 574 1054 1302 574
Size: 480 248 2895
Fragments arranged by size:
2895 480 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3623
NgoMIV G'CCGG_C
Cuts at: 1802 1802
Size: 3623
NspI r_CATG'y
Cuts at: 676 1176 2083 676
Size: 500 907 2216
Fragments arranged by size:
2216 907 500
PasI CC'CwG_GG
Cuts at: 153 267 290 153
Size: 114 23 3486
Fragments arranged by size:
3486 114 23
PciI A'CATG_T
Cuts at: 2079 2079
Size: 3623
PflMI CCAn_nnn'nTGG
Cuts at: 554 554
Size: 3623
PfoI T'CCnGG_A
Cuts at: 506 514 506
Size: 8 3615
PmeI GTTT'AAAC
Cuts at: 622 622
Size: 3623
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2762 2794 2762
Size: 32 3591
PstI C_TGCA'G
Cuts at: 670 1355 670
Size: 685 2938
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3623
PvuII CAG'CTG
Cuts at: 261 1048 1408 261
Size: 787 360 2476
Fragments arranged by size:
2476 787 360
RsrII CG'GwC_CG
Cuts at: 1818 1818
Size: 3623
SacI G_AGCT'C
Cuts at: 637 637
Size: 3623
SalI G'TCGA_C
Cuts at: 660 660
Size: 3623
SapI GCTCTTCn'nnn_
Cuts at: 476 537 1646 1856 476
Size: 61 1109 210 2243
Fragments arranged by size:
2243 1109 210 61
SbfI CC_TGCA'GG
Cuts at: 670 670
Size: 3623
ScaI AGT'ACT
Cuts at: 3140 3140
Size: 3623
SfcI C'TryA_G
Cuts at: 33 666 1351 2344 2535 33
Size: 633 685 993 191 1121
Fragments arranged by size:
1121 993 685 633 191
SfoI GGC'GCC
Cuts at: 575 1055 1303 575
Size: 480 248 2895
Fragments arranged by size:
2895 480 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3623
SgrAI Cr'CCGG_yG
Cuts at: 341 341
Size: 3623
SmaI CCC'GGG
Cuts at: 239 366 645 239
Size: 127 279 3217
Fragments arranged by size:
3217 279 127
SmlI C'TyrA_G
Cuts at: 778 1693 2185 2447 778
Size: 915 492 262 1954
Fragments arranged by size:
1954 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3623
SphI G_CATG'C
Cuts at: 676 1176 676
Size: 500 3123
StyI C'CwwG_G
Cuts at: 107 757 1111 107
Size: 650 354 2619
Fragments arranged by size:
2619 650 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1206 1339 1798 2007 1206
Size: 133 459 209 2822
Fragments arranged by size:
2822 459 209 133
TatI w'GTAC_w
Cuts at: 3138 3138
Size: 3623
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3140 3172 3140
Size: 32 3591
Tth111I GACn'n_nGTC
Cuts at: 1420 1420
Size: 3623
XbaI T'CTAG_A
Cuts at: 654 654
Size: 3623
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 258 483 1118 258
Size: 225 635 2763
Fragments arranged by size:
2763 635 225
XmaI C'CCGG_G
Cuts at: 237 364 643 237
Size: 127 279 3217
Fragments arranged by size:
3217 279 127
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI
NruI NsiI PacI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI