Restriction map for
pF1KE0593
(Circular) (Six-base) MAPSORT of: pF1KE0593.seq Check: 9613 from: 1 to: 3854
pF1KE0593 3854 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 196 434 196
Size: 238 3616
AatII G_ACGT'C
Cuts at: 697 697
Size: 3854
AccI GT'mk_AC
Cuts at: 892 892
Size: 3854
Acc65I G'GTAC_C
Cuts at: 870 870
Size: 3854
AclI AA'CG_TT
Cuts at: 3694 3694
Size: 3854
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1679 2111 2858 3126 1679
Size: 432 747 268 2407
Fragments arranged by size:
2407 747 432 268
AflIII A'CryG_T
Cuts at: 1206 2310 3287 1206
Size: 1104 977 1773
Fragments arranged by size:
1773 1104 977
AgeI A'CCGG_T
Cuts at: 2242 2242
Size: 3854
AleI CACnn'nnGTG
Cuts at: 794 794
Size: 3854
AlwNI CAG_nnn'CTG
Cuts at: 253 598 1159 2726 253
Size: 345 561 1567 1381
Fragments arranged by size:
1567 1381 561 345
ApaI G_GGCC'C
Cuts at: 486 486
Size: 3854
ApaLI G'TGCA_C
Cuts at: 2624 2624
Size: 3854
ApoI r'AATT_y
Cuts at: 858 3207 3463 858
Size: 2349 256 1249
Fragments arranged by size:
2349 1249 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3854
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3854
AvaI C'yCGr_G
Cuts at: 874 874
Size: 3854
BamHI G'GATC_C
Cuts at: 335 879 335
Size: 544 3310
BanI G'GyrC_C
Cuts at: 870 1284 1532 1567 870
Size: 414 248 35 3157
Fragments arranged by size:
3157 414 248 35
BanII G_rGCy'C
Cuts at: 211 486 735 868 1898 211
Size: 275 249 133 1030 2167
Fragments arranged by size:
2167 1030 275 249 133
BbeI G_GCGC'C
Cuts at: 1288 1536 1288
Size: 248 3606
BciVI GTATCCnnnnn_n'
Cuts at: 313 1752 2513 3854 313
Size: 1439 761 1341 313
Fragments arranged by size:
1439 1341 761 313
BclI T'GATC_A
Cuts at: 1374 1374
Size: 3854
BglI GCCn_nnn'nGGC
Cuts at: 289 373 730 289
Size: 84 357 3413
Fragments arranged by size:
3413 357 84
BglII A'GATC_T
Cuts at: 1369 3406 1369
Size: 2037 1817
BlpI GC'TnA_GC
Cuts at: 966 966
Size: 3854
Bme1580I G_kGCm'C
Cuts at: 115 146 405 486 587 1479 1572 2628 115
Size: 31 259 81 101 892 93 1056 1341
Fragments arranged by size:
1341 1056 892 259 101 93 81 31
BmgBI CAC'GTC
Cuts at: 672 672
Size: 3854
BmrI ACTGGGnnnn_n'
Cuts at: 537 1066 1147 1482 3626 537
Size: 529 81 335 2144 765
Fragments arranged by size:
2144 765 529 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2221 2221
Size: 3854
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 531 719 531
Size: 188 3666
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1030 1909 2401 2699 1030
Size: 879 492 298 2185
Fragments arranged by size:
2185 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3558 3558
Size: 3854
BsaAI yAC'GTr
Cuts at: 1207 1207
Size: 3854
BsaBI GATnn'nnATC
Cuts at: 6 340 1368 3405 6
Size: 334 1028 2037 455
Fragments arranged by size:
2037 1028 455 334
BsaHI Gr'CG_yC
Cuts at: 694 1285 1533 2235 3731 694
Size: 591 248 702 1496 817
Fragments arranged by size:
1496 817 702 591 248
BsaWI w'CCGG_w
Cuts at: 1043 1564 2242 2516 2663 3074 1043
Size: 521 678 274 147 411 1823
Fragments arranged by size:
1823 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 635 665 635
Size: 30 3824
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 215 215
Size: 3854
BseYI C'CCAG_C
Cuts at: 139 237 1279 2037 2614 3110 139
Size: 98 1042 758 577 496 883
Fragments arranged by size:
1042 883 758 577 496 98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 664 293
Size: 371 3483
BsiEI CG_ry'CG
Cuts at: 104 284 1396 1442 2650 104
Size: 180 1112 46 1208 1308
Fragments arranged by size:
1308 1208 1112 180 46
BsiHKAI G_wGCw'C
Cuts at: 868 1646 1836 2628 868
Size: 778 190 792 2094
Fragments arranged by size:
2094 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 115 146 211 405 486 587 735 868 1479
Size: 31 65 194 81 101 148 133 611
Cuts at: 1479 1572 1646 1836 1898 2628 115
Size: 93 74 190 62 730 1341
Fragments arranged by size:
1341 730 611 194 190 148 133 101
93 81 74 65 62 31
BspEI T'CCGG_A
Cuts at: 3074 3074
Size: 3854
BspHI T'CATG_A
Cuts at: 3030 3030
Size: 3854
BspMI ACCTGCnnnn'nnnn_
Cuts at: 196 434 904 1118 1420 1801 196
Size: 238 470 214 302 381 2249
Fragments arranged by size:
2249 470 381 302 238 214
BsrBI CCG'CTC
Cuts at: 50 2146 2200 3684 3849 50
Size: 2096 54 1484 165 55
Fragments arranged by size:
2096 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1766 1766
Size: 3854
BsrFI r'CCGG_y
Cuts at: 1852 2033 2242 1852
Size: 181 209 3464
Fragments arranged by size:
3464 209 181
BssSI C'ACGA_G
Cuts at: 2125 2483 2125
Size: 358 3496
BstAPI GCAn_nnn'nTGC
Cuts at: 1231 1231
Size: 3854
BstBI TT'CG_AA
Cuts at: 2215 2215
Size: 3854
BstXI CCAn_nnnn'nTGG
Cuts at: 139 446 139
Size: 307 3547
BstYI r'GATC_y
Cuts at: 1 335 879 1346 1369 1704 1950 2962 3048
Size: 334 544 467 23 335 246 1012 86
Cuts at: 3048 3060 3406 1
Size: 12 346 449
Fragments arranged by size:
1012 544 467 449 346 335 334 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 269 422 269
Size: 153 3701
BtgI C'CryG_G
Cuts at: 107 107
Size: 3854
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 165 1804 1985 2148 165
Size: 1639 181 163 1871
Fragments arranged by size:
1871 1639 181 163
BtsI GCAGTG_nn'
Cuts at: 1097 1097
Size: 3854
DraI TTT'AAA
Cuts at: 853 853
Size: 3854
DrdI GACnn_nn'nnGTC
Cuts at: 1560 2418 1560
Size: 858 2996
EaeI y'GGCC_r
Cuts at: 281 1439 1613 2004 2031 3376 281
Size: 1158 174 391 27 1345 759
Fragments arranged by size:
1345 1158 759 391 174 27
EagI C'GGCC_G
Cuts at: 281 1439 281
Size: 1158 2696
EarI CTCTTCn'nnn_
Cuts at: 542 1877 2087 542
Size: 1335 210 2309
Fragments arranged by size:
2309 1335 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2062 2372 2518 3664 2062
Size: 310 146 1146 2252
Fragments arranged by size:
2252 1146 310 146
EcoICRI GAG'CTC
Cuts at: 866 866
Size: 3854
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 531 719 1679 2111 2858 3126 531
Size: 188 960 432 747 268 1259
Fragments arranged by size:
1259 960 747 432 268 188
EcoNI CCTnn'n_nnAGG
Cuts at: 230 409 421 230
Size: 179 12 3663
Fragments arranged by size:
3663 179 12
EcoO109I rG'GnC_Cy
Cuts at: 414 431 482 993 414
Size: 17 51 511 3275
Fragments arranged by size:
3275 511 51 17
EcoRI G'AATT_C
Cuts at: 858 858
Size: 3854
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1873 1873
Size: 3854
FspI TGC'GCA
Cuts at: 1635 1635
Size: 3854
HaeII r_GCGC'y
Cuts at: 254 1288 1536 2558 3537 254
Size: 1034 248 1022 979 571
Fragments arranged by size:
1034 1022 979 571 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 210 242 635 667 1696 1726 1728 1758 210
Size: 32 393 32 1029 30 2 30 2306
Fragments arranged by size:
2306 1029 393 32 32 30 30 2
HincII GTy'rAC
Cuts at: 893 893
Size: 3854
KasI G'GCGC_C
Cuts at: 1284 1532 1284
Size: 248 3606
KpnI G_GTAC'C
Cuts at: 874 874
Size: 3854
MluI A'CGCG_T
Cuts at: 3287 3287
Size: 3854
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 496 2525 2709 496
Size: 2029 184 1641
Fragments arranged by size:
2029 1641 184
MscI TGG'CCA
Cuts at: 1615 1615
Size: 3854
MslI CAynn'nnrTG
Cuts at: 794 796 1843 1970 794
Size: 2 1047 127 2678
Fragments arranged by size:
2678 1047 127 2
MspA1I CmG'CkG
Cuts at: 965 1279 1639 2652 2897 965
Size: 314 360 1013 245 1922
Fragments arranged by size:
1922 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2035 2035
Size: 3854
NarI GG'CG_CC
Cuts at: 1285 1533 1285
Size: 248 3606
NcoI C'CATG_G
Cuts at: 107 107
Size: 3854
NgoMIV G'CCGG_C
Cuts at: 2033 2033
Size: 3854
NspI r_CATG'y
Cuts at: 907 1407 2314 907
Size: 500 907 2447
Fragments arranged by size:
2447 907 500
PasI CC'CwG_GG
Cuts at: 370 370
Size: 3854
PciI A'CATG_T
Cuts at: 2310 2310
Size: 3854
PflMI CCAn_nnn'nTGG
Cuts at: 478 478
Size: 3854
PmeI GTTT'AAAC
Cuts at: 853 853
Size: 3854
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2993 3025 2993
Size: 32 3822
PspOMI G'GGCC_C
Cuts at: 482 482
Size: 3854
PstI C_TGCA'G
Cuts at: 901 1586 901
Size: 685 3169
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3854
PvuII CAG'CTG
Cuts at: 1279 1639 1279
Size: 360 3494
RsrII CG'GwC_CG
Cuts at: 2049 2049
Size: 3854
SacI G_AGCT'C
Cuts at: 868 868
Size: 3854
SalI G'TCGA_C
Cuts at: 891 891
Size: 3854
SapI GCTCTTCn'nnn_
Cuts at: 1877 2087 1877
Size: 210 3644
SbfI CC_TGCA'GG
Cuts at: 901 901
Size: 3854
ScaI AGT'ACT
Cuts at: 3371 3371
Size: 3854
SfcI C'TryA_G
Cuts at: 33 897 1582 2575 2766 33
Size: 864 685 993 191 1121
Fragments arranged by size:
1121 993 864 685 191
SfoI GGC'GCC
Cuts at: 1286 1534 1286
Size: 248 3606
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3854
SmaI CCC'GGG
Cuts at: 876 876
Size: 3854
SmlI C'TyrA_G
Cuts at: 1009 1924 2416 2678 1009
Size: 915 492 262 2185
Fragments arranged by size:
2185 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3854
SphI G_CATG'C
Cuts at: 907 1407 907
Size: 500 3354
StyI C'CwwG_G
Cuts at: 107 810 988 1342 107
Size: 703 178 354 2619
Fragments arranged by size:
2619 703 354 178
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 638 784 1437 1570 2029 2238 638
Size: 146 653 133 459 209 2254
Fragments arranged by size:
2254 653 459 209 146 133
TatI w'GTAC_w
Cuts at: 3369 3369
Size: 3854
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3371 3403 3371
Size: 32 3822
Tth111I GACn'n_nGTC
Cuts at: 1651 1651
Size: 3854
XbaI T'CTAG_A
Cuts at: 885 885
Size: 3854
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 637 1349 637
Size: 712 3142
XmaI C'CCGG_G
Cuts at: 874 874
Size: 3854
XmnI GAAnn'nnTTC
Cuts at: 759 759
Size: 3854
ZraI GAC'GTC
Cuts at: 695 695
Size: 3854
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AloI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI