Restriction map for
pF1KE0589
(Circular) (Six-base) MAPSORT of: pF1KE0589.seq Check: 564 from: 1 to: 4499
pF1KE0589 4499 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 1537 1537
Size: 4499
Acc65I G'GTAC_C
Cuts at: 1515 1515
Size: 4499
AclI AA'CG_TT
Cuts at: 4339 4339
Size: 4499
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2324 2756 3503 3771 2324
Size: 432 747 268 3052
Fragments arranged by size:
3052 747 432 268
AflIII A'CryG_T
Cuts at: 1001 1247 1851 2955 3932 1001
Size: 246 604 1104 977 1568
Fragments arranged by size:
1568 1104 977 604 246
AgeI A'CCGG_T
Cuts at: 2887 2887
Size: 4499
AlwNI CAG_nnn'CTG
Cuts at: 360 1804 3371 360
Size: 1444 1567 1488
Fragments arranged by size:
1567 1488 1444
ApaLI G'TGCA_C
Cuts at: 3269 3269
Size: 4499
ApoI r'AATT_y
Cuts at: 1503 3852 4108 1503
Size: 2349 256 1894
Fragments arranged by size:
2349 1894 256
AscI GG'CGCG_CC
Cuts at: 652 652
Size: 4499
AseI AT'TA_AT
Cuts at: 20 20
Size: 4499
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
AvaI C'yCGr_G
Cuts at: 1157 1223 1519 1157
Size: 66 296 4137
Fragments arranged by size:
4137 296 66
BamHI G'GATC_C
Cuts at: 369 1524 369
Size: 1155 3344
BanI G'GyrC_C
Cuts at: 783 1218 1515 1929 2177 2212 783
Size: 435 297 414 248 35 3070
Fragments arranged by size:
3070 435 414 297 248 35
BanII G_rGCy'C
Cuts at: 1056 1513 2543 1056
Size: 457 1030 3012
Fragments arranged by size:
3012 1030 457
BbeI G_GCGC'C
Cuts at: 1222 1933 2181 1222
Size: 711 248 3540
Fragments arranged by size:
3540 711 248
BbsI GAAGACnn'nnnn_
Cuts at: 215 1221 215
Size: 1006 3493
BbvCI CC'TCA_GC
Cuts at: 274 274
Size: 4499
BciVI GTATCCnnnnn_n'
Cuts at: 2397 3158 4499 2397
Size: 761 1341 2397
Fragments arranged by size:
2397 1341 761
BclI T'GATC_A
Cuts at: 2019 2019
Size: 4499
BglII A'GATC_T
Cuts at: 2014 4051 2014
Size: 2037 2462
BlpI GC'TnA_GC
Cuts at: 514 1611 514
Size: 1097 3402
Bme1580I G_kGCm'C
Cuts at: 788 2124 2217 3273 788
Size: 1336 93 1056 2014
Fragments arranged by size:
2014 1336 1056 93
BmgBI CAC'GTC
Cuts at: 504 504
Size: 4499
BmrI ACTGGGnnnn_n'
Cuts at: 1711 1792 2127 4271 1711
Size: 81 335 2144 1939
Fragments arranged by size:
2144 1939 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2866 2866
Size: 4499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 838 1296 318
Size: 520 458 3521
Fragments arranged by size:
3521 520 458
Bpu10I CC'TnA_GC
Cuts at: 274 274
Size: 4499
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1030 1675 2554 3046 3344 1030
Size: 645 879 492 298 2185
Fragments arranged by size:
2185 879 645 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 284 4203 284
Size: 3919 580
BsaAI yAC'GTr
Cuts at: 1254 1852 1254
Size: 598 3901
BsaBI GATnn'nnATC
Cuts at: 6 853 2013 4050 6
Size: 847 1160 2037 455
Fragments arranged by size:
2037 1160 847 455
BsaHI Gr'CG_yC
Cuts at: 575 1219 1930 2178 2880 4376 575
Size: 644 711 248 702 1496 698
Fragments arranged by size:
1496 711 702 698 644 248
BsaWI w'CCGG_w
Cuts at: 1688 2209 2887 3161 3308 3719 1688
Size: 521 678 274 147 411 2468
Fragments arranged by size:
2468 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1146 1146
Size: 4499
BseYI C'CCAG_C
Cuts at: 302 606 1924 2682 3259 3755 302
Size: 304 1318 758 577 496 1046
Fragments arranged by size:
1318 1046 758 577 496 304
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 883 1398 883
Size: 515 3984
BsiEI CG_ry'CG
Cuts at: 104 2041 2087 3295 104
Size: 1937 46 1208 1308
Fragments arranged by size:
1937 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1174 1513 2291 2481 3273 1174
Size: 339 778 190 792 2400
Fragments arranged by size:
2400 792 778 339 190
BsmI GAATG_Cn'
Cuts at: 246 246
Size: 4499
Bsp1286I G_dGCh'C
Cuts at: 788 1056 1174 1513 2124 2217 2291 2481 2543
Size: 268 118 339 611 93 74 190 62
Cuts at: 2543 3273 788
Size: 730 2014
Fragments arranged by size:
2014 730 611 339 268 190 118 93
74 62
BspEI T'CCGG_A
Cuts at: 3719 3719
Size: 4499
BspHI T'CATG_A
Cuts at: 3675 3675
Size: 4499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1415 1549 1763 2065 2446 1415
Size: 134 214 302 381 3468
Fragments arranged by size:
3468 381 302 214 134
BsrBI CCG'CTC
Cuts at: 50 2791 2845 4329 4494 50
Size: 2741 54 1484 165 55
Fragments arranged by size:
2741 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 941 2411 941
Size: 1470 3029
BsrFI r'CCGG_y
Cuts at: 973 1121 2497 2678 2887 973
Size: 148 1376 181 209 2585
Fragments arranged by size:
2585 1376 209 181 148
BssHII G'CGCG_C
Cuts at: 652 652
Size: 4499
BssSI C'ACGA_G
Cuts at: 476 2770 3128 476
Size: 2294 358 1847
Fragments arranged by size:
2294 1847 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1876 1876
Size: 4499
BstBI TT'CG_AA
Cuts at: 2860 2860
Size: 4499
BstEII G'GTnAC_C
Cuts at: 1098 1098
Size: 4499
BstXI CCAn_nnnn'nTGG
Cuts at: 816 1032 1185 1311 816
Size: 216 153 126 4004
Fragments arranged by size:
4004 216 153 126
BstYI r'GATC_y
Cuts at: 1 140 369 1524 1991 2014 2349 2595 3607
Size: 139 229 1155 467 23 335 246 1012
Cuts at: 3607 3693 3705 4051 1
Size: 86 12 346 449
Fragments arranged by size:
1155 1012 467 449 346 335 246 229
139 86 23 12
BtgI C'CryG_G
Cuts at: 128 128
Size: 4499
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 437 2449 2630 2793 437
Size: 2012 181 163 2143
Fragments arranged by size:
2143 2012 181 163
BtsI GCAGTG_nn'
Cuts at: 173 647 773 878 1742 173
Size: 474 126 105 864 2930
Fragments arranged by size:
2930 864 474 126 105
DraI TTT'AAA
Cuts at: 1498 1498
Size: 4499
DrdI GACnn_nn'nnGTC
Cuts at: 1279 2205 3063 1279
Size: 926 858 2715
Fragments arranged by size:
2715 926 858
EaeI y'GGCC_r
Cuts at: 468 538 806 1109 1301 1313 2084 2258 2649
Size: 70 268 303 192 12 771 174 391
Cuts at: 2649 2676 4021 468
Size: 27 1345 946
Fragments arranged by size:
1345 946 771 391 303 268 192 174
70 27 12
EagI C'GGCC_G
Cuts at: 2084 2084
Size: 4499
EarI CTCTTCn'nnn_
Cuts at: 2522 2732 2522
Size: 210 4289
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 334 2707 3017 3163 4309 334
Size: 2373 310 146 1146 524
Fragments arranged by size:
2373 1146 524 310 146
EcoICRI GAG'CTC
Cuts at: 1511 1511
Size: 4499
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 838 1296 2324 2756 3503 3771 318
Size: 520 458 1028 432 747 268 1046
Fragments arranged by size:
1046 1028 747 520 458 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 757 757
Size: 4499
EcoO109I rG'GnC_Cy
Cuts at: 150 306 445 557 677 1041 1638 150
Size: 156 139 112 120 364 597 3011
Fragments arranged by size:
3011 597 364 156 139 120 112
EcoRI G'AATT_C
Cuts at: 1503 1503
Size: 4499
EcoRV GAT'ATC
Cuts at: 717 717
Size: 4499
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2518 2518
Size: 4499
FspI TGC'GCA
Cuts at: 802 2280 802
Size: 1478 3021
FspAI rTGC'GCAy
Cuts at: 802 802
Size: 4499
HaeII r_GCGC'y
Cuts at: 703 1222 1933 2181 3203 4182 703
Size: 519 711 248 1022 979 1020
Fragments arranged by size:
1022 1020 979 711 519 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1003 1035 1376 1408 1449 1481 2341 2371 2373
Size: 32 341 32 41 32 860 30 2
Cuts at: 2373 2403 1003
Size: 30 3099
Fragments arranged by size:
3099 860 341 41 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 870 971 1538 870
Size: 101 567 3831
Fragments arranged by size:
3831 567 101
KasI G'GCGC_C
Cuts at: 1218 1929 2177 1218
Size: 711 248 3540
Fragments arranged by size:
3540 711 248
KpnI G_GTAC'C
Cuts at: 1519 1519
Size: 4499
MluI A'CGCG_T
Cuts at: 3932 3932
Size: 4499
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1407 3170 3354 1407
Size: 1763 184 2552
Fragments arranged by size:
2552 1763 184
MscI TGG'CCA
Cuts at: 470 540 1303 1315 2260 470
Size: 70 763 12 945 2709
Fragments arranged by size:
2709 945 763 70 12
MslI CAynn'nnrTG
Cuts at: 727 1252 2488 2615 727
Size: 525 1236 127 2611
Fragments arranged by size:
2611 1236 525 127
MspA1I CmG'CkG
Cuts at: 418 466 509 1610 1924 2284 3297 3542 418
Size: 48 43 1101 314 360 1013 245 1375
Fragments arranged by size:
1375 1101 1013 360 314 245 48 43
NaeI GCC'GGC
Cuts at: 2680 2680
Size: 4499
NarI GG'CG_CC
Cuts at: 1219 1930 2178 1219
Size: 711 248 3540
Fragments arranged by size:
3540 711 248
NcoI C'CATG_G
Cuts at: 128 128
Size: 4499
NgoMIV G'CCGG_C
Cuts at: 2678 2678
Size: 4499
NspI r_CATG'y
Cuts at: 1005 1251 1552 2052 2959 1005
Size: 246 301 500 907 2545
Fragments arranged by size:
2545 907 500 301 246
PciI A'CATG_T
Cuts at: 1001 1247 2955 1001
Size: 246 1708 2545
Fragments arranged by size:
2545 1708 246
PflMI CCAn_nnn'nTGG
Cuts at: 795 795
Size: 4499
PfoI T'CCnGG_A
Cuts at: 815 1467 815
Size: 652 3847
PmeI GTTT'AAAC
Cuts at: 1498 1498
Size: 4499
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3638 3670 3638
Size: 32 4467
PpuMI rG'GwC_Cy
Cuts at: 150 306 445 557 1041 150
Size: 156 139 112 484 3608
Fragments arranged by size:
3608 484 156 139 112
PstI C_TGCA'G
Cuts at: 167 761 1154 1546 2231 167
Size: 594 393 392 685 2435
Fragments arranged by size:
2435 685 594 393 392
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4499
PvuII CAG'CTG
Cuts at: 418 466 1924 2284 418
Size: 48 1458 360 2633
Fragments arranged by size:
2633 1458 360 48
RsrII CG'GwC_CG
Cuts at: 2694 2694
Size: 4499
SacI G_AGCT'C
Cuts at: 1513 1513
Size: 4499
SalI G'TCGA_C
Cuts at: 1536 1536
Size: 4499
SanDI GG'GwC_CC
Cuts at: 150 445 150
Size: 295 4204
SapI GCTCTTCn'nnn_
Cuts at: 2522 2732 2522
Size: 210 4289
SbfI CC_TGCA'GG
Cuts at: 167 761 1546 167
Size: 594 785 3120
Fragments arranged by size:
3120 785 594
ScaI AGT'ACT
Cuts at: 4016 4016
Size: 4499
SfcI C'TryA_G
Cuts at: 33 163 594 757 1150 1542 2227 3220 3411
Size: 130 431 163 393 392 685 993 191
Cuts at: 3411 33
Size: 1121
Fragments arranged by size:
1121 993 685 431 393 392 191 163
130
SfoI GGC'GCC
Cuts at: 1220 1931 2179 1220
Size: 711 248 3540
Fragments arranged by size:
3540 711 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
SmaI CCC'GGG
Cuts at: 1159 1225 1521 1159
Size: 66 296 4137
Fragments arranged by size:
4137 296 66
SmlI C'TyrA_G
Cuts at: 1045 1654 2569 3061 3323 1045
Size: 609 915 492 262 2221
Fragments arranged by size:
2221 915 609 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4499
SphI G_CATG'C
Cuts at: 1552 2052 1552
Size: 500 3999
StyI C'CwwG_G
Cuts at: 128 326 399 1292 1633 1987 128
Size: 198 73 893 341 354 2640
Fragments arranged by size:
2640 893 354 341 198 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1038 2082 2215 2674 2883 1038
Size: 1044 133 459 209 2654
Fragments arranged by size:
2654 1044 459 209 133
TatI w'GTAC_w
Cuts at: 4014 4014
Size: 4499
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4016 4048 4016
Size: 32 4467
Tth111I GACn'n_nGTC
Cuts at: 1039 2296 1039
Size: 1257 3242
XbaI T'CTAG_A
Cuts at: 1530 1530
Size: 4499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1994 1994
Size: 4499
XmaI C'CCGG_G
Cuts at: 1157 1223 1519 1157
Size: 66 296 4137
Fragments arranged by size:
4137 296 66
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AvrII BaeI BcgI BfrBI BglI BmtI BsaXI
BsiWI BsmBI BsrGI BstZ17I Bsu36I ClaI CspCI DraIII
FseI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI