Restriction map for
pF1KE0585
(Circular) (Six-base) MAPSORT of: pF1KE0585.seq Check: 6075 from: 1 to: 4019
pF1KE0585 4019 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AatII G_ACGT'C
Cuts at: 962 962
Size: 4019
AccI GT'mk_AC
Cuts at: 780 1057 780
Size: 277 3742
Acc65I G'GTAC_C
Cuts at: 1035 1035
Size: 4019
AclI AA'CG_TT
Cuts at: 3859 3859
Size: 4019
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 869 1844 2276 3023 3291 869
Size: 975 432 747 268 1597
Fragments arranged by size:
1597 975 747 432 268
AflII C'TTAA_G
Cuts at: 364 364
Size: 4019
AflIII A'CryG_T
Cuts at: 713 1371 2475 3452 713
Size: 658 1104 977 1280
Fragments arranged by size:
1280 1104 977 658
AgeI A'CCGG_T
Cuts at: 2407 2407
Size: 4019
AhdI GACnn_n'nnGTC
Cuts at: 765 958 765
Size: 193 3826
AleI CACnn'nnGTG
Cuts at: 625 625
Size: 4019
AlwNI CAG_nnn'CTG
Cuts at: 454 622 1324 2891 454
Size: 168 702 1567 1582
Fragments arranged by size:
1582 1567 702 168
ApaLI G'TGCA_C
Cuts at: 2789 2789
Size: 4019
ApoI r'AATT_y
Cuts at: 831 1023 3372 3628 831
Size: 192 2349 256 1222
Fragments arranged by size:
2349 1222 256 192
AseI AT'TA_AT
Cuts at: 20 20
Size: 4019
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4019
AvaI C'yCGr_G
Cuts at: 1039 1039
Size: 4019
BamHI G'GATC_C
Cuts at: 1044 1044
Size: 4019
BanI G'GyrC_C
Cuts at: 1035 1449 1697 1732 1035
Size: 414 248 35 3322
Fragments arranged by size:
3322 414 248 35
BanII G_rGCy'C
Cuts at: 1033 2063 1033
Size: 1030 2989
BbeI G_GCGC'C
Cuts at: 1453 1701 1453
Size: 248 3771
BciVI GTATCCnnnnn_n'
Cuts at: 736 1917 2678 4019 736
Size: 1181 761 1341 736
Fragments arranged by size:
1341 1181 761 736
BclI T'GATC_A
Cuts at: 473 1539 473
Size: 1066 2953
BfrBI ATG'CAT
Cuts at: 403 564 582 993 997 403
Size: 161 18 411 4 3425
Fragments arranged by size:
3425 411 161 18 4
BglII A'GATC_T
Cuts at: 357 642 1534 3571 357
Size: 285 892 2037 805
Fragments arranged by size:
2037 892 805 285
BlpI GC'TnA_GC
Cuts at: 1131 1131
Size: 4019
Bme1580I G_kGCm'C
Cuts at: 1644 1737 2793 1644
Size: 93 1056 2870
Fragments arranged by size:
2870 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 632 1231 1312 1647 3791 632
Size: 599 81 335 2144 860
Fragments arranged by size:
2144 860 599 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2386 2386
Size: 4019
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 704 704
Size: 4019
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1195 2074 2566 2864 1195
Size: 879 492 298 2350
Fragments arranged by size:
2350 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 773 3723 773
Size: 2950 1069
BsaAI yAC'GTr
Cuts at: 334 1372 334
Size: 1038 2981
BsaBI GATnn'nnATC
Cuts at: 6 1533 3570 6
Size: 1527 2037 455
Fragments arranged by size:
2037 1527 455
BsaHI Gr'CG_yC
Cuts at: 959 1450 1698 2400 3896 959
Size: 491 248 702 1496 1082
Fragments arranged by size:
1496 1082 702 491 248
BsaWI w'CCGG_w
Cuts at: 177 1208 1729 2407 2681 2828 3239 177
Size: 1031 521 678 274 147 411 957
Fragments arranged by size:
1031 957 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 269 299 269
Size: 30 3989
BseYI C'CCAG_C
Cuts at: 1444 2202 2779 3275 1444
Size: 758 577 496 2188
Fragments arranged by size:
2188 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 668 322
Size: 346 3673
BsiEI CG_ry'CG
Cuts at: 104 178 1561 1607 2815 104
Size: 74 1383 46 1208 1308
Fragments arranged by size:
1383 1308 1208 74 46
BsiHKAI G_wGCw'C
Cuts at: 1033 1811 2001 2793 1033
Size: 778 190 792 2259
Fragments arranged by size:
2259 792 778 190
BsmI GAATG_Cn'
Cuts at: 134 564 134
Size: 430 3589
Bsp1286I G_dGCh'C
Cuts at: 1033 1644 1737 1811 2001 2063 2793 1033
Size: 611 93 74 190 62 730 2259
Fragments arranged by size:
2259 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3239 3239
Size: 4019
BspHI T'CATG_A
Cuts at: 3195 3195
Size: 4019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1069 1283 1585 1966 1069
Size: 214 302 381 3122
Fragments arranged by size:
3122 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2311 2365 3849 4014 50
Size: 2261 54 1484 165 55
Fragments arranged by size:
2261 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1931 1931
Size: 4019
BsrFI r'CCGG_y
Cuts at: 656 2017 2198 2407 656
Size: 1361 181 209 2268
Fragments arranged by size:
2268 1361 209 181
BssSI C'ACGA_G
Cuts at: 2290 2648 2290
Size: 358 3661
BstAPI GCAn_nnn'nTGC
Cuts at: 1003 1396 1003
Size: 393 3626
BstBI TT'CG_AA
Cuts at: 210 2380 210
Size: 2170 1849
BstXI CCAn_nnnn'nTGG
Cuts at: 627 627
Size: 4019
BstYI r'GATC_y
Cuts at: 1 357 642 939 1044 1511 1534 1869 2115
Size: 356 285 297 105 467 23 335 246
Cuts at: 2115 3127 3213 3225 3571 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 356 346 335 297 285
246 105 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4019
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1969 2150 2313 1969
Size: 181 163 3675
Fragments arranged by size:
3675 181 163
BtsI GCAGTG_nn'
Cuts at: 552 1001 1262 552
Size: 449 261 3309
Fragments arranged by size:
3309 449 261
DraI TTT'AAA
Cuts at: 293 1018 293
Size: 725 3294
DrdI GACnn_nn'nnGTC
Cuts at: 1725 2583 1725
Size: 858 3161
EaeI y'GGCC_r
Cuts at: 654 1604 1778 2169 2196 3541 654
Size: 950 174 391 27 1345 1132
Fragments arranged by size:
1345 1132 950 391 174 27
EagI C'GGCC_G
Cuts at: 1604 1604
Size: 4019
EarI CTCTTCn'nnn_
Cuts at: 567 884 2042 2252 567
Size: 317 1158 210 2334
Fragments arranged by size:
2334 1158 317 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2227 2537 2683 3829 2227
Size: 310 146 1146 2417
Fragments arranged by size:
2417 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1031 1031
Size: 4019
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 704 869 1844 2276 3023 3291 704
Size: 165 975 432 747 268 1432
Fragments arranged by size:
1432 975 747 432 268 165
EcoO109I rG'GnC_Cy
Cuts at: 1158 1158
Size: 4019
EcoRI G'AATT_C
Cuts at: 1023 1023
Size: 4019
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2038 2038
Size: 4019
FspI TGC'GCA
Cuts at: 1800 1800
Size: 4019
HaeII r_GCGC'y
Cuts at: 1453 1701 2723 3702 1453
Size: 248 1022 979 1770
Fragments arranged by size:
1770 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 103 135 751 783 944 976 1861 1891 1893
Size: 32 616 32 161 32 885 30 2
Cuts at: 1893 1923 103
Size: 30 2199
Fragments arranged by size:
2199 885 616 161 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1058 1058
Size: 4019
KasI G'GCGC_C
Cuts at: 1449 1697 1449
Size: 248 3771
KpnI G_GTAC'C
Cuts at: 1039 1039
Size: 4019
MluI A'CGCG_T
Cuts at: 3452 3452
Size: 4019
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 180 933 2690 2874 180
Size: 753 1757 184 1325
Fragments arranged by size:
1757 1325 753 184
MscI TGG'CCA
Cuts at: 1780 1780
Size: 4019
MslI CAynn'nnrTG
Cuts at: 383 625 737 2008 2135 383
Size: 242 112 1271 127 2267
Fragments arranged by size:
2267 1271 242 127 112
MspA1I CmG'CkG
Cuts at: 682 1130 1444 1804 2817 3062 682
Size: 448 314 360 1013 245 1639
Fragments arranged by size:
1639 1013 448 360 314 245
NaeI GCC'GGC
Cuts at: 2200 2200
Size: 4019
NarI GG'CG_CC
Cuts at: 1450 1698 1450
Size: 248 3771
NcoI C'CATG_G
Cuts at: 107 107
Size: 4019
NgoMIV G'CCGG_C
Cuts at: 2198 2198
Size: 4019
NsiI A_TGCA'T
Cuts at: 405 566 584 995 999 405
Size: 161 18 411 4 3425
Fragments arranged by size:
3425 411 161 18 4
NspI r_CATG'y
Cuts at: 564 717 997 1001 1072 1572 2479 564
Size: 153 280 4 71 500 907 2104
Fragments arranged by size:
2104 907 500 280 153 71 4
PciI A'CATG_T
Cuts at: 713 2475 713
Size: 1762 2257
PmeI GTTT'AAAC
Cuts at: 1018 1018
Size: 4019
PmlI CAC'GTG
Cuts at: 334 334
Size: 4019
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3158 3190 3158
Size: 32 3987
PstI C_TGCA'G
Cuts at: 1066 1751 1066
Size: 685 3334
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4019
PvuII CAG'CTG
Cuts at: 682 1444 1804 682
Size: 762 360 2897
Fragments arranged by size:
2897 762 360
RsrII CG'GwC_CG
Cuts at: 2214 2214
Size: 4019
SacI G_AGCT'C
Cuts at: 1033 1033
Size: 4019
SalI G'TCGA_C
Cuts at: 1056 1056
Size: 4019
SapI GCTCTTCn'nnn_
Cuts at: 2042 2252 2042
Size: 210 3809
SbfI CC_TGCA'GG
Cuts at: 1066 1066
Size: 4019
ScaI AGT'ACT
Cuts at: 3536 3536
Size: 4019
SfcI C'TryA_G
Cuts at: 33 1062 1747 2740 2931 33
Size: 1029 685 993 191 1121
Fragments arranged by size:
1121 1029 993 685 191
SfoI GGC'GCC
Cuts at: 1451 1699 1451
Size: 248 3771
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4019
SmaI CCC'GGG
Cuts at: 1041 1041
Size: 4019
SmlI C'TyrA_G
Cuts at: 364 1174 2089 2581 2843 364
Size: 810 915 492 262 1540
Fragments arranged by size:
1540 915 810 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4019
SphI G_CATG'C
Cuts at: 997 1001 1072 1572 997
Size: 4 71 500 3444
Fragments arranged by size:
3444 500 71 4
SspI AAT'ATT
Cuts at: 397 397
Size: 4019
StuI AGG'CCT
Cuts at: 169 169
Size: 4019
StyI C'CwwG_G
Cuts at: 107 1153 1507 107
Size: 1046 354 2619
Fragments arranged by size:
2619 1046 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1602 1735 2194 2403 1602
Size: 133 459 209 3218
Fragments arranged by size:
3218 459 209 133
TatI w'GTAC_w
Cuts at: 3534 3534
Size: 4019
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3536 3568 3536
Size: 32 3987
Tth111I GACn'n_nGTC
Cuts at: 1816 1816
Size: 4019
XbaI T'CTAG_A
Cuts at: 1050 1050
Size: 4019
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1514 1514
Size: 4019
XmaI C'CCGG_G
Cuts at: 1039 1039
Size: 4019
ZraI GAC'GTC
Cuts at: 960 960
Size: 4019
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PmeI PmlI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AlfI AloI ApaI AscI AvrII BaeI
BbsI BbvCI BcgI BglI BmgBI BmtI Bpu10I BseRI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI PacI PasI PflMI
PfoI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
XmnI