Restriction map for
pF1KE0584
(Circular) (Six-base) MAPSORT of: pF1KE0584.seq Check: 785 from: 1 to: 3611
pF1KE0584 3611 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 278 278
Size: 3611
AccI GT'mk_AC
Cuts at: 649 649
Size: 3611
Acc65I G'GTAC_C
Cuts at: 627 627
Size: 3611
AclI AA'CG_TT
Cuts at: 3451 3451
Size: 3611
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 330 1436 1868 2615 2883 330
Size: 1106 432 747 268 1058
Fragments arranged by size:
1106 1058 747 432 268
AflIII A'CryG_T
Cuts at: 963 2067 3044 963
Size: 1104 977 1530
Fragments arranged by size:
1530 1104 977
AgeI A'CCGG_T
Cuts at: 1999 1999
Size: 3611
AleI CACnn'nnGTG
Cuts at: 601 601
Size: 3611
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 316 316
Size: 3611
AlwNI CAG_nnn'CTG
Cuts at: 916 2483 916
Size: 1567 2044
ApaLI G'TGCA_C
Cuts at: 2381 2381
Size: 3611
ApoI r'AATT_y
Cuts at: 615 2964 3220 615
Size: 2349 256 1006
Fragments arranged by size:
2349 1006 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3611
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3611
AvaI C'yCGr_G
Cuts at: 510 631 510
Size: 121 3490
BamHI G'GATC_C
Cuts at: 636 636
Size: 3611
BanI G'GyrC_C
Cuts at: 627 1041 1289 1324 627
Size: 414 248 35 2914
Fragments arranged by size:
2914 414 248 35
BanII G_rGCy'C
Cuts at: 404 625 1655 404
Size: 221 1030 2360
Fragments arranged by size:
2360 1030 221
BbeI G_GCGC'C
Cuts at: 1045 1293 1045
Size: 248 3363
BciVI GTATCCnnnnn_n'
Cuts at: 1509 2270 3611 1509
Size: 761 1341 1509
Fragments arranged by size:
1509 1341 761
BclI T'GATC_A
Cuts at: 1131 1131
Size: 3611
BglII A'GATC_T
Cuts at: 1126 3163 1126
Size: 2037 1574
BlpI GC'TnA_GC
Cuts at: 723 723
Size: 3611
Bme1580I G_kGCm'C
Cuts at: 1236 1329 2385 1236
Size: 93 1056 2462
Fragments arranged by size:
2462 1056 93
BmgBI CAC'GTC
Cuts at: 447 498 603 447
Size: 51 105 3455
Fragments arranged by size:
3455 105 51
BmrI ACTGGGnnnn_n'
Cuts at: 823 904 1239 3383 823
Size: 81 335 2144 1051
Fragments arranged by size:
2144 1051 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1978 1978
Size: 3611
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 181
Size: 3611
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 787 1666 2158 2456 787
Size: 879 492 298 1942
Fragments arranged by size:
1942 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3315 3315
Size: 3611
BsaAI yAC'GTr
Cuts at: 964 964
Size: 3611
BsaBI GATnn'nnATC
Cuts at: 6 1125 3162 6
Size: 1119 2037 455
Fragments arranged by size:
2037 1119 455
BsaHI Gr'CG_yC
Cuts at: 1042 1290 1992 3488 1042
Size: 248 702 1496 1165
Fragments arranged by size:
1496 1165 702 248
BsaWI w'CCGG_w
Cuts at: 800 1321 1999 2273 2420 2831 800
Size: 521 678 274 147 411 1580
Fragments arranged by size:
1580 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 244 253 448 244
Size: 9 195 3407
Fragments arranged by size:
3407 195 9
BseYI C'CCAG_C
Cuts at: 1036 1794 2371 2867 1036
Size: 758 577 496 1780
Fragments arranged by size:
1780 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 265
Size: 3611
BsiEI CG_ry'CG
Cuts at: 104 1153 1199 2407 104
Size: 1049 46 1208 1308
Fragments arranged by size:
1308 1208 1049 46
BsiHKAI G_wGCw'C
Cuts at: 473 625 1403 1593 2385 473
Size: 152 778 190 792 1699
Fragments arranged by size:
1699 792 778 190 152
Bsp1286I G_dGCh'C
Cuts at: 404 473 625 1236 1329 1403 1593 1655 2385
Size: 69 152 611 93 74 190 62 730
Cuts at: 2385 404
Size: 1630
Fragments arranged by size:
1630 730 611 190 152 93 74 69
62
BspEI T'CCGG_A
Cuts at: 2831 2831
Size: 3611
BspHI T'CATG_A
Cuts at: 2787 2787
Size: 3611
BspMI ACCTGCnnnn'nnnn_
Cuts at: 278 661 875 1177 1558 278
Size: 383 214 302 381 2331
Fragments arranged by size:
2331 383 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1903 1957 3441 3606 50
Size: 1853 54 1484 165 55
Fragments arranged by size:
1853 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1523 1523
Size: 3611
BsrFI r'CCGG_y
Cuts at: 191 315 1609 1790 1999 191
Size: 124 1294 181 209 1803
Fragments arranged by size:
1803 1294 209 181 124
BssSI C'ACGA_G
Cuts at: 1882 2240 1882
Size: 358 3253
BstAPI GCAn_nnn'nTGC
Cuts at: 988 988
Size: 3611
BstBI TT'CG_AA
Cuts at: 1972 1972
Size: 3611
BstYI r'GATC_y
Cuts at: 1 636 1103 1126 1461 1707 2719 2805 2817
Size: 635 467 23 335 246 1012 86 12
Cuts at: 2817 3163 1
Size: 346 449
Fragments arranged by size:
1012 635 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 122 122
Size: 3611
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 156 1561 1742 1905 156
Size: 1405 181 163 1862
Fragments arranged by size:
1862 1405 181 163
BtsI GCAGTG_nn'
Cuts at: 393 854 393
Size: 461 3150
DraI TTT'AAA
Cuts at: 610 610
Size: 3611
DrdI GACnn_nn'nnGTC
Cuts at: 1317 2175 1317
Size: 858 2753
EaeI y'GGCC_r
Cuts at: 1196 1370 1761 1788 3133 1196
Size: 174 391 27 1345 1674
Fragments arranged by size:
1674 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1196 1196
Size: 3611
EarI CTCTTCn'nnn_
Cuts at: 434 1634 1844 434
Size: 1200 210 2201
Fragments arranged by size:
2201 1200 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1819 2129 2275 3421 1819
Size: 310 146 1146 2009
Fragments arranged by size:
2009 1146 310 146
EcoICRI GAG'CTC
Cuts at: 623 623
Size: 3611
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 330 1436 1868 2615 2883 181
Size: 149 1106 432 747 268 909
Fragments arranged by size:
1106 909 747 432 268 149
EcoNI CCTnn'n_nnAGG
Cuts at: 576 576
Size: 3611
EcoO109I rG'GnC_Cy
Cuts at: 525 750 525
Size: 225 3386
EcoRI G'AATT_C
Cuts at: 615 615
Size: 3611
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1630 1630
Size: 3611
FspI TGC'GCA
Cuts at: 1392 1392
Size: 3611
HaeII r_GCGC'y
Cuts at: 322 538 1045 1293 2315 3294 322
Size: 216 507 248 1022 979 639
Fragments arranged by size:
1022 979 639 507 248 216
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1453 1483 1485 1515 1453
Size: 30 2 30 3549
Fragments arranged by size:
3549 30 30 2
HincII GTy'rAC
Cuts at: 650 650
Size: 3611
KasI G'GCGC_C
Cuts at: 1041 1289 1041
Size: 248 3363
KpnI G_GTAC'C
Cuts at: 631 631
Size: 3611
MluI A'CGCG_T
Cuts at: 3044 3044
Size: 3611
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 206 311 2282 2466 206
Size: 105 1971 184 1351
Fragments arranged by size:
1971 1351 184 105
MscI TGG'CCA
Cuts at: 1372 1372
Size: 3611
MslI CAynn'nnrTG
Cuts at: 601 1600 1727 601
Size: 999 127 2485
Fragments arranged by size:
2485 999 127
MspA1I CmG'CkG
Cuts at: 279 386 722 1036 1396 2409 2654 279
Size: 107 336 314 360 1013 245 1236
Fragments arranged by size:
1236 1013 360 336 314 245 107
NaeI GCC'GGC
Cuts at: 193 317 1792 193
Size: 124 1475 2012
Fragments arranged by size:
2012 1475 124
NarI GG'CG_CC
Cuts at: 1042 1290 1042
Size: 248 3363
NgoMIV G'CCGG_C
Cuts at: 191 315 1790 191
Size: 124 1475 2012
Fragments arranged by size:
2012 1475 124
NspI r_CATG'y
Cuts at: 664 1164 2071 664
Size: 500 907 2204
Fragments arranged by size:
2204 907 500
PciI A'CATG_T
Cuts at: 2067 2067
Size: 3611
PmeI GTTT'AAAC
Cuts at: 610 610
Size: 3611
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2750 2782 2750
Size: 32 3579
PshAI GACnn'nnGTC
Cuts at: 124 124
Size: 3611
PstI C_TGCA'G
Cuts at: 422 533 658 1343 422
Size: 111 125 685 2690
Fragments arranged by size:
2690 685 125 111
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3611
PvuII CAG'CTG
Cuts at: 386 1036 1396 386
Size: 650 360 2601
Fragments arranged by size:
2601 650 360
RsrII CG'GwC_CG
Cuts at: 1806 1806
Size: 3611
SacI G_AGCT'C
Cuts at: 625 625
Size: 3611
SalI G'TCGA_C
Cuts at: 648 648
Size: 3611
SapI GCTCTTCn'nnn_
Cuts at: 1634 1844 1634
Size: 210 3401
SbfI CC_TGCA'GG
Cuts at: 422 533 658 422
Size: 111 125 3375
Fragments arranged by size:
3375 125 111
ScaI AGT'ACT
Cuts at: 202 3128 202
Size: 2926 685
SexAI A'CCwGG_T
Cuts at: 296 296
Size: 3611
SfcI C'TryA_G
Cuts at: 33 418 529 654 1339 2332 2523 33
Size: 385 111 125 685 993 191 1121
Fragments arranged by size:
1121 993 685 385 191 125 111
SfoI GGC'GCC
Cuts at: 1043 1291 1043
Size: 248 3363
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3611
SmaI CCC'GGG
Cuts at: 512 633 512
Size: 121 3490
SmlI C'TyrA_G
Cuts at: 766 1681 2173 2435 766
Size: 915 492 262 1942
Fragments arranged by size:
1942 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3611
SphI G_CATG'C
Cuts at: 664 1164 664
Size: 500 3111
StuI AGG'CCT
Cuts at: 571 571
Size: 3611
StyI C'CwwG_G
Cuts at: 745 1099 745
Size: 354 3257
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1194 1327 1786 1995 1194
Size: 133 459 209 2810
Fragments arranged by size:
2810 459 209 133
TatI w'GTAC_w
Cuts at: 200 3126 200
Size: 2926 685
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3128 3160 3128
Size: 32 3579
Tth111I GACn'n_nGTC
Cuts at: 1408 1408
Size: 3611
XbaI T'CTAG_A
Cuts at: 642 642
Size: 3611
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 294 1106 294
Size: 812 2799
XmaI C'CCGG_G
Cuts at: 510 631 510
Size: 121 3490
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PpiI PshAI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmtI Bpu10I
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI