Restriction map for
pF1KE0582
(Circular) (Six-base) MAPSORT of: pF1KE0582.seq Check: 7461 from: 1 to: 4031
pF1KE0582 4031 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 1069 1069
Size: 4031
Acc65I G'GTAC_C
Cuts at: 1047 1047
Size: 4031
AclI AA'CG_TT
Cuts at: 3871 3871
Size: 4031
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 803 1856 2288 3035 3303 803
Size: 1053 432 747 268 1531
Fragments arranged by size:
1531 1053 747 432 268
AflIII A'CryG_T
Cuts at: 1383 2487 3464 1383
Size: 1104 977 1950
Fragments arranged by size:
1950 1104 977
AgeI A'CCGG_T
Cuts at: 2419 2419
Size: 4031
AlwNI CAG_nnn'CTG
Cuts at: 1336 2903 1336
Size: 1567 2464
ApaLI G'TGCA_C
Cuts at: 2801 2801
Size: 4031
ApoI r'AATT_y
Cuts at: 124 166 539 1035 3384 3640 124
Size: 42 373 496 2349 256 515
Fragments arranged by size:
2349 515 496 373 256 42
AseI AT'TA_AT
Cuts at: 20 20
Size: 4031
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4031
AvaI C'yCGr_G
Cuts at: 1051 1051
Size: 4031
BamHI G'GATC_C
Cuts at: 1056 1056
Size: 4031
BanI G'GyrC_C
Cuts at: 1047 1461 1709 1744 1047
Size: 414 248 35 3334
Fragments arranged by size:
3334 414 248 35
BanII G_rGCy'C
Cuts at: 1045 2075 1045
Size: 1030 3001
BbeI G_GCGC'C
Cuts at: 1465 1713 1465
Size: 248 3783
BciVI GTATCCnnnnn_n'
Cuts at: 1929 2690 4031 1929
Size: 761 1341 1929
Fragments arranged by size:
1929 1341 761
BclI T'GATC_A
Cuts at: 200 1551 200
Size: 1351 2680
BglII A'GATC_T
Cuts at: 1546 3583 1546
Size: 2037 1994
BlpI GC'TnA_GC
Cuts at: 1143 1143
Size: 4031
Bme1580I G_kGCm'C
Cuts at: 1656 1749 2805 1656
Size: 93 1056 2882
Fragments arranged by size:
2882 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 579 1243 1324 1659 3803 579
Size: 664 81 335 2144 807
Fragments arranged by size:
2144 807 664 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2398 2398
Size: 4031
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 667
Size: 4031
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1207 2086 2578 2876 1207
Size: 879 492 298 2362
Fragments arranged by size:
2362 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 418 776 3735 418
Size: 358 2959 714
Fragments arranged by size:
2959 714 358
BsaAI yAC'GTr
Cuts at: 1384 1384
Size: 4031
BsaBI GATnn'nnATC
Cuts at: 6 1545 3582 6
Size: 1539 2037 455
Fragments arranged by size:
2037 1539 455
BsaHI Gr'CG_yC
Cuts at: 1462 1710 2412 3908 1462
Size: 248 702 1496 1585
Fragments arranged by size:
1585 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1220 1741 2419 2693 2840 3251 1220
Size: 521 678 274 147 411 2000
Fragments arranged by size:
2000 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 135 249 1456 2214 2791 3287 135
Size: 114 1207 758 577 496 879
Fragments arranged by size:
1207 879 758 577 496 114
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 642 642
Size: 4031
BsiEI CG_ry'CG
Cuts at: 104 1573 1619 2827 104
Size: 1469 46 1208 1308
Fragments arranged by size:
1469 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 763 1045 1823 2013 2805 763
Size: 282 778 190 792 1989
Fragments arranged by size:
1989 792 778 282 190
Bsp1286I G_dGCh'C
Cuts at: 763 1045 1656 1749 1823 2013 2075 2805 763
Size: 282 611 93 74 190 62 730 1989
Fragments arranged by size:
1989 730 611 282 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3251 3251
Size: 4031
BspHI T'CATG_A
Cuts at: 497 3207 497
Size: 2710 1321
BspMI ACCTGCnnnn'nnnn_
Cuts at: 202 227 1081 1295 1597 1978 202
Size: 25 854 214 302 381 2255
Fragments arranged by size:
2255 854 381 302 214 25
BsrBI CCG'CTC
Cuts at: 50 2323 2377 3861 4026 50
Size: 2273 54 1484 165 55
Fragments arranged by size:
2273 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1943 1943
Size: 4031
BsrFI r'CCGG_y
Cuts at: 2029 2210 2419 2029
Size: 181 209 3641
Fragments arranged by size:
3641 209 181
BssSI C'ACGA_G
Cuts at: 2302 2660 2302
Size: 358 3673
BstAPI GCAn_nnn'nTGC
Cuts at: 1408 1408
Size: 4031
BstBI TT'CG_AA
Cuts at: 2392 2392
Size: 4031
BstEII G'GTnAC_C
Cuts at: 214 214
Size: 4031
BstYI r'GATC_y
Cuts at: 1 1056 1523 1546 1881 2127 3139 3225 3237
Size: 1055 467 23 335 246 1012 86 12
Cuts at: 3237 3583 1
Size: 346 449
Fragments arranged by size:
1055 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 766 766
Size: 4031
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1981 2162 2325 1981
Size: 181 163 3687
Fragments arranged by size:
3687 181 163
BtsI GCAGTG_nn'
Cuts at: 977 1274 977
Size: 297 3734
DraI TTT'AAA
Cuts at: 1030 1030
Size: 4031
DrdI GACnn_nn'nnGTC
Cuts at: 1737 2595 1737
Size: 858 3173
EaeI y'GGCC_r
Cuts at: 464 1616 1790 2181 2208 3553 464
Size: 1152 174 391 27 1345 942
Fragments arranged by size:
1345 1152 942 391 174 27
EagI C'GGCC_G
Cuts at: 1616 1616
Size: 4031
EarI CTCTTCn'nnn_
Cuts at: 318 400 647 2054 2264 318
Size: 82 247 1407 210 2085
Fragments arranged by size:
2085 1407 247 210 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2239 2549 2695 3841 2239
Size: 310 146 1146 2429
Fragments arranged by size:
2429 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1043 1043
Size: 4031
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 803 1856 2288 3035 3303 667
Size: 136 1053 432 747 268 1395
Fragments arranged by size:
1395 1053 747 432 268 136
EcoO109I rG'GnC_Cy
Cuts at: 679 1170 679
Size: 491 3540
EcoRI G'AATT_C
Cuts at: 124 539 1035 124
Size: 415 496 3120
Fragments arranged by size:
3120 496 415
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2050 2050
Size: 4031
FspI TGC'GCA
Cuts at: 1812 1812
Size: 4031
HaeII r_GCGC'y
Cuts at: 1465 1713 2735 3714 1465
Size: 248 1022 979 1782
Fragments arranged by size:
1782 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 334 366 1873 1903 1905 1935 334
Size: 32 1507 30 2 30 2430
Fragments arranged by size:
2430 1507 32 30 30 2
HincII GTy'rAC
Cuts at: 1070 1070
Size: 4031
KasI G'GCGC_C
Cuts at: 1461 1709 1461
Size: 248 3783
KpnI G_GTAC'C
Cuts at: 1051 1051
Size: 4031
MluI A'CGCG_T
Cuts at: 3464 3464
Size: 4031
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2702 2886 2702
Size: 184 3847
MscI TGG'CCA
Cuts at: 466 1792 466
Size: 1326 2705
MslI CAynn'nnrTG
Cuts at: 2020 2147 2020
Size: 127 3904
MspA1I CmG'CkG
Cuts at: 421 1142 1456 1816 2829 3074 421
Size: 721 314 360 1013 245 1378
Fragments arranged by size:
1378 1013 721 360 314 245
NaeI GCC'GGC
Cuts at: 2212 2212
Size: 4031
NarI GG'CG_CC
Cuts at: 1462 1710 1462
Size: 248 3783
NgoMIV G'CCGG_C
Cuts at: 2210 2210
Size: 4031
NspI r_CATG'y
Cuts at: 1084 1584 2491 1084
Size: 500 907 2624
Fragments arranged by size:
2624 907 500
PasI CC'CwG_GG
Cuts at: 255 255
Size: 4031
PciI A'CATG_T
Cuts at: 2487 2487
Size: 4031
PflMI CCAn_nnn'nTGG
Cuts at: 598 598
Size: 4031
PmeI GTTT'AAAC
Cuts at: 1030 1030
Size: 4031
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3170 3202 3170
Size: 32 3999
PstI C_TGCA'G
Cuts at: 164 1078 1763 164
Size: 914 685 2432
Fragments arranged by size:
2432 914 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4031
PvuII CAG'CTG
Cuts at: 421 1456 1816 421
Size: 1035 360 2636
Fragments arranged by size:
2636 1035 360
RsrII CG'GwC_CG
Cuts at: 2226 2226
Size: 4031
SacI G_AGCT'C
Cuts at: 1045 1045
Size: 4031
SalI G'TCGA_C
Cuts at: 1068 1068
Size: 4031
SapI GCTCTTCn'nnn_
Cuts at: 400 2054 2264 400
Size: 1654 210 2167
Fragments arranged by size:
2167 1654 210
SbfI CC_TGCA'GG
Cuts at: 1078 1078
Size: 4031
ScaI AGT'ACT
Cuts at: 3548 3548
Size: 4031
SfcI C'TryA_G
Cuts at: 33 160 1074 1759 2752 2943 33
Size: 127 914 685 993 191 1121
Fragments arranged by size:
1121 993 914 685 191 127
SfoI GGC'GCC
Cuts at: 1463 1711 1463
Size: 248 3783
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4031
SmaI CCC'GGG
Cuts at: 1053 1053
Size: 4031
SmlI C'TyrA_G
Cuts at: 1186 2101 2593 2855 1186
Size: 915 492 262 2362
Fragments arranged by size:
2362 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4031
SphI G_CATG'C
Cuts at: 1084 1584 1084
Size: 500 3531
StyI C'CwwG_G
Cuts at: 1165 1519 1165
Size: 354 3677
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1614 1747 2206 2415 1614
Size: 133 459 209 3230
Fragments arranged by size:
3230 459 209 133
TatI w'GTAC_w
Cuts at: 3546 3546
Size: 4031
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 135 167 698 730 3548 3580 135
Size: 32 531 32 2818 32 586
Fragments arranged by size:
2818 586 531 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1828 1828
Size: 4031
XbaI T'CTAG_A
Cuts at: 1062 1062
Size: 4031
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 511 1526 511
Size: 1015 3016
XmaI C'CCGG_G
Cuts at: 1051 1051
Size: 4031
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BseRI BsiWI BsmI
BsmBI BsrGI BssHII BstXI BstZ17I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI