Restriction map for
pF1KE0581
(Circular) (Six-base) MAPSORT of: pF1KE0581.seq Check: 2095 from: 1 to: 3962
pF1KE0581 3962 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 1000 1000
Size: 3962
Acc65I G'GTAC_C
Cuts at: 978 978
Size: 3962
AclI AA'CG_TT
Cuts at: 3802 3802
Size: 3962
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1787 2219 2966 3234 1787
Size: 432 747 268 2515
Fragments arranged by size:
2515 747 432 268
AfeI AGC'GCT
Cuts at: 155 155
Size: 3962
AflIII A'CryG_T
Cuts at: 1314 2418 3395 1314
Size: 1104 977 1881
Fragments arranged by size:
1881 1104 977
AgeI A'CCGG_T
Cuts at: 2350 2350
Size: 3962
AleI CACnn'nnGTG
Cuts at: 538 538
Size: 3962
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 550 582 550
Size: 32 3930
AlwNI CAG_nnn'CTG
Cuts at: 732 930 1267 2834 732
Size: 198 337 1567 1860
Fragments arranged by size:
1860 1567 337 198
ApaI G_GGCC'C
Cuts at: 547 547
Size: 3962
ApaLI G'TGCA_C
Cuts at: 2732 2732
Size: 3962
ApoI r'AATT_y
Cuts at: 966 3315 3571 966
Size: 2349 256 1357
Fragments arranged by size:
2349 1357 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3962
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3962
AvaI C'yCGr_G
Cuts at: 982 982
Size: 3962
BamHI G'GATC_C
Cuts at: 377 987 377
Size: 610 3352
BanI G'GyrC_C
Cuts at: 978 1392 1640 1675 978
Size: 414 248 35 3265
Fragments arranged by size:
3265 414 248 35
BanII G_rGCy'C
Cuts at: 446 547 809 851 976 2006 446
Size: 101 262 42 125 1030 2402
Fragments arranged by size:
2402 1030 262 125 101 42
BbeI G_GCGC'C
Cuts at: 1396 1644 1396
Size: 248 3714
BbsI GAAGACnn'nnnn_
Cuts at: 198 198
Size: 3962
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3928
BciVI GTATCCnnnnn_n'
Cuts at: 1860 2621 3962 1860
Size: 761 1341 1860
Fragments arranged by size:
1860 1341 761
BclI T'GATC_A
Cuts at: 716 1482 716
Size: 766 3196
BglII A'GATC_T
Cuts at: 1477 3514 1477
Size: 2037 1925
BlpI GC'TnA_GC
Cuts at: 751 1074 751
Size: 323 3639
Bme1580I G_kGCm'C
Cuts at: 547 620 1587 1680 2736 547
Size: 73 967 93 1056 1773
Fragments arranged by size:
1773 1056 967 93 73
BmrI ACTGGGnnnn_n'
Cuts at: 1174 1255 1590 3734 1174
Size: 81 335 2144 1402
Fragments arranged by size:
2144 1402 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2329 2329
Size: 3962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 264 439 163
Size: 101 175 3686
Fragments arranged by size:
3686 175 101
Bpu10I CC'TnA_GC
Cuts at: 835 835
Size: 3962
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1138 2017 2509 2807 1138
Size: 879 492 298 2293
Fragments arranged by size:
2293 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 520 3666 520
Size: 3146 816
BsaAI yAC'GTr
Cuts at: 1315 1315
Size: 3962
BsaBI GATnn'nnATC
Cuts at: 6 1476 3513 6
Size: 1470 2037 455
Fragments arranged by size:
2037 1470 455
BsaHI Gr'CG_yC
Cuts at: 1393 1641 2343 3839 1393
Size: 248 702 1496 1516
Fragments arranged by size:
1516 1496 702 248
BsaWI w'CCGG_w
Cuts at: 299 1151 1672 2350 2624 2771 3182 299
Size: 852 521 678 274 147 411 1079
Fragments arranged by size:
1079 852 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 437 598 859 1387 2145 2722 3218 437
Size: 161 261 528 758 577 496 1181
Fragments arranged by size:
1181 758 577 528 496 261 161
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 408 516 718 408
Size: 108 202 3652
Fragments arranged by size:
3652 202 108
BsiEI CG_ry'CG
Cuts at: 104 1504 1550 2758 104
Size: 1400 46 1208 1308
Fragments arranged by size:
1400 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 293 446 976 1754 1944 2736 293
Size: 153 530 778 190 792 1519
Fragments arranged by size:
1519 792 778 530 190 153
Bsp1286I G_dGCh'C
Cuts at: 293 446 547 620 809 851 976 1587 1680
Size: 153 101 73 189 42 125 611 93
Cuts at: 1680 1754 1944 2006 2736 293
Size: 74 190 62 730 1519
Fragments arranged by size:
1519 730 611 190 189 153 125 101
93 74 73 62 42
BspEI T'CCGG_A
Cuts at: 3182 3182
Size: 3962
BspHI T'CATG_A
Cuts at: 3138 3138
Size: 3962
BspMI ACCTGCnnnn'nnnn_
Cuts at: 538 1012 1226 1528 1909 538
Size: 474 214 302 381 2591
Fragments arranged by size:
2591 474 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2254 2308 3792 3957 50
Size: 2204 54 1484 165 55
Fragments arranged by size:
2204 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1874 1874
Size: 3962
BsrFI r'CCGG_y
Cuts at: 773 1960 2141 2350 773
Size: 1187 181 209 2385
Fragments arranged by size:
2385 1187 209 181
BssHII G'CGCG_C
Cuts at: 789 789
Size: 3962
BssSI C'ACGA_G
Cuts at: 2233 2591 2233
Size: 358 3604
BstAPI GCAn_nnn'nTGC
Cuts at: 732 1339 732
Size: 607 3355
BstBI TT'CG_AA
Cuts at: 2323 2323
Size: 3962
BstXI CCAn_nnnn'nTGG
Cuts at: 684 867 684
Size: 183 3779
BstYI r'GATC_y
Cuts at: 1 377 467 987 1454 1477 1812 2058 3070
Size: 376 90 520 467 23 335 246 1012
Cuts at: 3070 3156 3168 3514 1
Size: 86 12 346 449
Fragments arranged by size:
1012 520 467 449 376 346 335 246
90 86 23 12
BtgI C'CryG_G
Cuts at: 167 167
Size: 3962
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 795 1912 2093 2256 795
Size: 1117 181 163 2501
Fragments arranged by size:
2501 1117 181 163
BtsI GCAGTG_nn'
Cuts at: 1205 1205
Size: 3962
ClaI AT'CG_AT
Cuts at: 269 269
Size: 3962
DraI TTT'AAA
Cuts at: 961 961
Size: 3962
DrdI GACnn_nn'nnGTC
Cuts at: 1668 2526 1668
Size: 858 3104
EaeI y'GGCC_r
Cuts at: 638 689 1547 1721 2112 2139 3484 638
Size: 51 858 174 391 27 1345 1116
Fragments arranged by size:
1345 1116 858 391 174 51 27
EagI C'GGCC_G
Cuts at: 1547 1547
Size: 3962
EarI CTCTTCn'nnn_
Cuts at: 1985 2195 1985
Size: 210 3752
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2170 2480 2626 3772 2170
Size: 310 146 1146 2360
Fragments arranged by size:
2360 1146 310 146
EcoICRI GAG'CTC
Cuts at: 444 974 444
Size: 530 3432
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 264 439 1787 2219 2966 3234 163
Size: 101 175 1348 432 747 268 891
Fragments arranged by size:
1348 891 747 432 268 175 101
EcoNI CCTnn'n_nnAGG
Cuts at: 457 457
Size: 3962
EcoO109I rG'GnC_Cy
Cuts at: 544 897 1101 544
Size: 353 204 3405
Fragments arranged by size:
3405 353 204
EcoRI G'AATT_C
Cuts at: 966 966
Size: 3962
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1981 1981
Size: 3962
FspI TGC'GCA
Cuts at: 1743 1743
Size: 3962
HaeII r_GCGC'y
Cuts at: 157 866 1396 1644 2666 3645 157
Size: 709 530 248 1022 979 474
Fragments arranged by size:
1022 979 709 530 474 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 262 294 1804 1834 1836 1866 262
Size: 32 1510 30 2 30 2358
Fragments arranged by size:
2358 1510 32 30 30 2
HincII GTy'rAC
Cuts at: 1001 1001
Size: 3962
KasI G'GCGC_C
Cuts at: 1392 1640 1392
Size: 248 3714
KpnI G_GTAC'C
Cuts at: 982 982
Size: 3962
MluI A'CGCG_T
Cuts at: 3395 3395
Size: 3962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2633 2817 2633
Size: 184 3778
MscI TGG'CCA
Cuts at: 640 691 1723 640
Size: 51 1032 2879
Fragments arranged by size:
2879 1032 51
MslI CAynn'nnrTG
Cuts at: 538 577 760 1951 2078 538
Size: 39 183 1191 127 2422
Fragments arranged by size:
2422 1191 183 127 39
MspA1I CmG'CkG
Cuts at: 437 930 1073 1387 1747 2760 3005 437
Size: 493 143 314 360 1013 245 1394
Fragments arranged by size:
1394 1013 493 360 314 245 143
NaeI GCC'GGC
Cuts at: 2143 2143
Size: 3962
NarI GG'CG_CC
Cuts at: 1393 1641 1393
Size: 248 3714
NcoI C'CATG_G
Cuts at: 167 167
Size: 3962
NgoMIV G'CCGG_C
Cuts at: 2141 2141
Size: 3962
NspI r_CATG'y
Cuts at: 432 747 1015 1515 2422 432
Size: 315 268 500 907 1972
Fragments arranged by size:
1972 907 500 315 268
PasI CC'CwG_GG
Cuts at: 780 780
Size: 3962
PciI A'CATG_T
Cuts at: 2418 2418
Size: 3962
PfoI T'CCnGG_A
Cuts at: 209 455 209
Size: 246 3716
PmeI GTTT'AAAC
Cuts at: 961 961
Size: 3962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3101 3133 3101
Size: 32 3930
PpuMI rG'GwC_Cy
Cuts at: 897 897
Size: 3962
PspOMI G'GGCC_C
Cuts at: 543 543
Size: 3962
PstI C_TGCA'G
Cuts at: 728 1009 1694 728
Size: 281 685 2996
Fragments arranged by size:
2996 685 281
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3962
PvuII CAG'CTG
Cuts at: 437 1387 1747 437
Size: 950 360 2652
Fragments arranged by size:
2652 950 360
RsrII CG'GwC_CG
Cuts at: 2157 2157
Size: 3962
SacI G_AGCT'C
Cuts at: 446 976 446
Size: 530 3432
SalI G'TCGA_C
Cuts at: 999 999
Size: 3962
SapI GCTCTTCn'nnn_
Cuts at: 1985 2195 1985
Size: 210 3752
SbfI CC_TGCA'GG
Cuts at: 1009 1009
Size: 3962
ScaI AGT'ACT
Cuts at: 3479 3479
Size: 3962
SfcI C'TryA_G
Cuts at: 33 724 1005 1690 2683 2874 33
Size: 691 281 685 993 191 1121
Fragments arranged by size:
1121 993 691 685 281 191
SfoI GGC'GCC
Cuts at: 1394 1642 1394
Size: 248 3714
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3962
SmaI CCC'GGG
Cuts at: 984 984
Size: 3962
SmlI C'TyrA_G
Cuts at: 1117 2032 2524 2786 1117
Size: 915 492 262 2293
Fragments arranged by size:
2293 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3962
SphI G_CATG'C
Cuts at: 747 1015 1515 747
Size: 268 500 3194
Fragments arranged by size:
3194 500 268
StyI C'CwwG_G
Cuts at: 167 1096 1450 167
Size: 929 354 2679
Fragments arranged by size:
2679 929 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1545 1678 2137 2346 1545
Size: 133 459 209 3161
Fragments arranged by size:
3161 459 209 133
TatI w'GTAC_w
Cuts at: 3477 3477
Size: 3962
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 296 328 3479 3511 296
Size: 32 3151 32 747
Fragments arranged by size:
3151 747 32 32
Tth111I GACn'n_nGTC
Cuts at: 253 1759 253
Size: 1506 2456
XbaI T'CTAG_A
Cuts at: 993 993
Size: 3962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 684 777 1457 684
Size: 93 680 3189
Fragments arranged by size:
3189 680 93
XmaI C'CCGG_G
Cuts at: 982 982
Size: 3962
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AscI AvrII BaeI
BbvCI BfrBI BglI BmgBI BmtI BsaXI BseRI BsiWI
BsmI BsmBI BsrGI BstEII BstZ17I Bsu36I CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI