Restriction map for
pF1KE0580
(Circular) (Six-base) MAPSORT of: pF1KE0580.seq Check: 9048 from: 1 to: 3581
pF1KE0580 3581 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 619 619
Size: 3581
Acc65I G'GTAC_C
Cuts at: 597 597
Size: 3581
AclI AA'CG_TT
Cuts at: 3421 3421
Size: 3581
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 1406 1838 2585 2853 167
Size: 1239 432 747 268 895
Fragments arranged by size:
1239 895 747 432 268
AflIII A'CryG_T
Cuts at: 933 2037 3014 933
Size: 1104 977 1500
Fragments arranged by size:
1500 1104 977
AgeI A'CCGG_T
Cuts at: 1969 1969
Size: 3581
AlwNI CAG_nnn'CTG
Cuts at: 886 2453 886
Size: 1567 2014
ApaLI G'TGCA_C
Cuts at: 2351 2351
Size: 3581
ApoI r'AATT_y
Cuts at: 585 2934 3190 585
Size: 2349 256 976
Fragments arranged by size:
2349 976 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3581
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3581
AvaI C'yCGr_G
Cuts at: 601 601
Size: 3581
BamHI G'GATC_C
Cuts at: 606 606
Size: 3581
BanI G'GyrC_C
Cuts at: 597 1011 1259 1294 597
Size: 414 248 35 2884
Fragments arranged by size:
2884 414 248 35
BanII G_rGCy'C
Cuts at: 595 1625 595
Size: 1030 2551
BbeI G_GCGC'C
Cuts at: 1015 1263 1015
Size: 248 3333
BbsI GAAGACnn'nnnn_
Cuts at: 229 229
Size: 3581
BciVI GTATCCnnnnn_n'
Cuts at: 1479 2240 3581 1479
Size: 761 1341 1479
Fragments arranged by size:
1479 1341 761
BclI T'GATC_A
Cuts at: 1101 1101
Size: 3581
BglI GCCn_nnn'nGGC
Cuts at: 184 184
Size: 3581
BglII A'GATC_T
Cuts at: 1096 3133 1096
Size: 2037 1544
BlpI GC'TnA_GC
Cuts at: 693 693
Size: 3581
Bme1580I G_kGCm'C
Cuts at: 1206 1299 2355 1206
Size: 93 1056 2432
Fragments arranged by size:
2432 1056 93
BmgBI CAC'GTC
Cuts at: 233 233
Size: 3581
BmrI ACTGGGnnnn_n'
Cuts at: 793 874 1209 3353 793
Size: 81 335 2144 1021
Fragments arranged by size:
2144 1021 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1948 1948
Size: 3581
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 757 1636 2128 2426 757
Size: 879 492 298 1912
Fragments arranged by size:
1912 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3285 3285
Size: 3581
BsaAI yAC'GTr
Cuts at: 934 934
Size: 3581
BsaBI GATnn'nnATC
Cuts at: 6 1095 3132 6
Size: 1089 2037 455
Fragments arranged by size:
2037 1089 455
BsaHI Gr'CG_yC
Cuts at: 267 1012 1260 1962 3458 267
Size: 745 248 702 1496 390
Fragments arranged by size:
1496 745 702 390 248
BsaWI w'CCGG_w
Cuts at: 770 1291 1969 2243 2390 2801 770
Size: 521 678 274 147 411 1550
Fragments arranged by size:
1550 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1006 1764 2341 2837 1006
Size: 758 577 496 1750
Fragments arranged by size:
1750 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 365 1123 1169 2377 104
Size: 261 758 46 1208 1308
Fragments arranged by size:
1308 1208 758 261 46
BsiHKAI G_wGCw'C
Cuts at: 301 595 1373 1563 2355 301
Size: 294 778 190 792 1527
Fragments arranged by size:
1527 792 778 294 190
Bsp1286I G_dGCh'C
Cuts at: 301 595 1206 1299 1373 1563 1625 2355 301
Size: 294 611 93 74 190 62 730 1527
Fragments arranged by size:
1527 730 611 294 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2801 2801
Size: 3581
BspHI T'CATG_A
Cuts at: 2757 2757
Size: 3581
BspMI ACCTGCnnnn'nnnn_
Cuts at: 631 845 1147 1528 631
Size: 214 302 381 2684
Fragments arranged by size:
2684 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1873 1927 3411 3576 50
Size: 1823 54 1484 165 55
Fragments arranged by size:
1823 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1493 1493
Size: 3581
BsrFI r'CCGG_y
Cuts at: 1579 1760 1969 1579
Size: 181 209 3191
Fragments arranged by size:
3191 209 181
BssSI C'ACGA_G
Cuts at: 1852 2210 1852
Size: 358 3223
BstAPI GCAn_nnn'nTGC
Cuts at: 958 958
Size: 3581
BstBI TT'CG_AA
Cuts at: 1942 1942
Size: 3581
BstEII G'GTnAC_C
Cuts at: 477 477
Size: 3581
BstYI r'GATC_y
Cuts at: 1 606 1073 1096 1431 1677 2689 2775 2787
Size: 605 467 23 335 246 1012 86 12
Cuts at: 2787 3133 1
Size: 346 449
Fragments arranged by size:
1012 605 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 353 107
Size: 246 3335
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1531 1712 1875 1531
Size: 181 163 3237
Fragments arranged by size:
3237 181 163
BtsI GCAGTG_nn'
Cuts at: 259 824 259
Size: 565 3016
DraI TTT'AAA
Cuts at: 173 580 173
Size: 407 3174
DrdI GACnn_nn'nnGTC
Cuts at: 231 357 1287 2145 231
Size: 126 930 858 1667
Fragments arranged by size:
1667 930 858 126
EaeI y'GGCC_r
Cuts at: 321 362 470 1166 1340 1731 1758 3103 321
Size: 41 108 696 174 391 27 1345 799
Fragments arranged by size:
1345 799 696 391 174 108 41 27
EagI C'GGCC_G
Cuts at: 362 1166 362
Size: 804 2777
EarI CTCTTCn'nnn_
Cuts at: 289 364 1604 1814 289
Size: 75 1240 210 2056
Fragments arranged by size:
2056 1240 210 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 482 1789 2099 2245 3391 482
Size: 1307 310 146 1146 672
Fragments arranged by size:
1307 1146 672 310 146
EcoICRI GAG'CTC
Cuts at: 593 593
Size: 3581
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 1406 1838 2585 2853 167
Size: 1239 432 747 268 895
Fragments arranged by size:
1239 895 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 720 720
Size: 3581
EcoRI G'AATT_C
Cuts at: 585 585
Size: 3581
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1600 1600
Size: 3581
FspI TGC'GCA
Cuts at: 1362 1362
Size: 3581
HaeII r_GCGC'y
Cuts at: 115 1015 1263 2285 3264 115
Size: 900 248 1022 979 432
Fragments arranged by size:
1022 979 900 432 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1423 1453 1455 1485 1423
Size: 30 2 30 3519
Fragments arranged by size:
3519 30 30 2
HincII GTy'rAC
Cuts at: 620 620
Size: 3581
HindIII A'AGCT_T
Cuts at: 316 316
Size: 3581
KasI G'GCGC_C
Cuts at: 1011 1259 1011
Size: 248 3333
KpnI G_GTAC'C
Cuts at: 601 601
Size: 3581
MluI A'CGCG_T
Cuts at: 3014 3014
Size: 3581
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 527 2252 2436 527
Size: 1725 184 1672
Fragments arranged by size:
1725 1672 184
MscI TGG'CCA
Cuts at: 323 1342 323
Size: 1019 2562
MslI CAynn'nnrTG
Cuts at: 1570 1697 1570
Size: 127 3454
MspA1I CmG'CkG
Cuts at: 249 348 692 1006 1366 2379 2624 249
Size: 99 344 314 360 1013 245 1206
Fragments arranged by size:
1206 1013 360 344 314 245 99
NaeI GCC'GGC
Cuts at: 1762 1762
Size: 3581
NarI GG'CG_CC
Cuts at: 1012 1260 1012
Size: 248 3333
NcoI C'CATG_G
Cuts at: 107 107
Size: 3581
NgoMIV G'CCGG_C
Cuts at: 1760 1760
Size: 3581
NspI r_CATG'y
Cuts at: 518 634 1134 2041 518
Size: 116 500 907 2058
Fragments arranged by size:
2058 907 500 116
PciI A'CATG_T
Cuts at: 2037 2037
Size: 3581
PmeI GTTT'AAAC
Cuts at: 580 580
Size: 3581
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2720 2752 2720
Size: 32 3549
PstI C_TGCA'G
Cuts at: 628 1313 628
Size: 685 2896
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3581
PvuII CAG'CTG
Cuts at: 1006 1366 1006
Size: 360 3221
RsrII CG'GwC_CG
Cuts at: 1776 1776
Size: 3581
SacI G_AGCT'C
Cuts at: 595 595
Size: 3581
SalI G'TCGA_C
Cuts at: 618 618
Size: 3581
SapI GCTCTTCn'nnn_
Cuts at: 289 1604 1814 289
Size: 1315 210 2056
Fragments arranged by size:
2056 1315 210
SbfI CC_TGCA'GG
Cuts at: 628 628
Size: 3581
ScaI AGT'ACT
Cuts at: 3098 3098
Size: 3581
SfcI C'TryA_G
Cuts at: 33 624 1309 2302 2493 33
Size: 591 685 993 191 1121
Fragments arranged by size:
1121 993 685 591 191
SfoI GGC'GCC
Cuts at: 1013 1261 1013
Size: 248 3333
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3581
SmaI CCC'GGG
Cuts at: 603 603
Size: 3581
SmlI C'TyrA_G
Cuts at: 736 1651 2143 2405 736
Size: 915 492 262 1912
Fragments arranged by size:
1912 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3581
SphI G_CATG'C
Cuts at: 634 1134 634
Size: 500 3081
StyI C'CwwG_G
Cuts at: 107 289 715 1069 107
Size: 182 426 354 2619
Fragments arranged by size:
2619 426 354 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1164 1297 1756 1965 1164
Size: 133 459 209 2780
Fragments arranged by size:
2780 459 209 133
TatI w'GTAC_w
Cuts at: 3096 3096
Size: 3581
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 227 259 474 506 3098 3130 227
Size: 32 215 32 2592 32 678
Fragments arranged by size:
2592 678 215 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1378 1378
Size: 3581
XbaI T'CTAG_A
Cuts at: 612 612
Size: 3581
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1076 1076
Size: 3581
XmaI C'CCGG_G
Cuts at: 601 601
Size: 3581
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmtI
BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI