Restriction map for
pF1KE0579
(Circular) (Six-base) MAPSORT of: pF1KE0579.seq Check: 8027 from: 1 to: 3950
pF1KE0579 3950 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 988 988
Size: 3950
Acc65I G'GTAC_C
Cuts at: 966 966
Size: 3950
AclI AA'CG_TT
Cuts at: 3790 3790
Size: 3950
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 794 1775 2207 2954 3222 794
Size: 981 432 747 268 1522
Fragments arranged by size:
1522 981 747 432 268
AflIII A'CryG_T
Cuts at: 1302 2406 3383 1302
Size: 1104 977 1869
Fragments arranged by size:
1869 1104 977
AgeI A'CCGG_T
Cuts at: 2338 2338
Size: 3950
AleI CACnn'nnGTG
Cuts at: 163 163
Size: 3950
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 139 139
Size: 3950
AlwNI CAG_nnn'CTG
Cuts at: 490 1255 2822 490
Size: 765 1567 1618
Fragments arranged by size:
1618 1567 765
ApaLI G'TGCA_C
Cuts at: 2720 2720
Size: 3950
ApoI r'AATT_y
Cuts at: 256 954 3303 3559 256
Size: 698 2349 256 647
Fragments arranged by size:
2349 698 647 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3950
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3950
AvaI C'yCGr_G
Cuts at: 970 970
Size: 3950
BamHI G'GATC_C
Cuts at: 975 975
Size: 3950
BanI G'GyrC_C
Cuts at: 170 966 1380 1628 1663 170
Size: 796 414 248 35 2457
Fragments arranged by size:
2457 796 414 248 35
BanII G_rGCy'C
Cuts at: 537 964 1994 537
Size: 427 1030 2493
Fragments arranged by size:
2493 1030 427
BbeI G_GCGC'C
Cuts at: 174 1384 1632 174
Size: 1210 248 2492
Fragments arranged by size:
2492 1210 248
BbsI GAAGACnn'nnnn_
Cuts at: 333 676 831 333
Size: 343 155 3452
Fragments arranged by size:
3452 343 155
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 608 642 608
Size: 34 3916
BciVI GTATCCnnnnn_n'
Cuts at: 1848 2609 3950 1848
Size: 761 1341 1848
Fragments arranged by size:
1848 1341 761
BclI T'GATC_A
Cuts at: 1470 1470
Size: 3950
BglII A'GATC_T
Cuts at: 1465 3502 1465
Size: 2037 1913
BlpI GC'TnA_GC
Cuts at: 524 903 1062 524
Size: 379 159 3412
Fragments arranged by size:
3412 379 159
Bme1580I G_kGCm'C
Cuts at: 1575 1668 2724 1575
Size: 93 1056 2801
Fragments arranged by size:
2801 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 423 847 1162 1243 1578 3722 423
Size: 424 315 81 335 2144 651
Fragments arranged by size:
2144 651 424 335 315 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2317 2317
Size: 3950
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 568 251
Size: 317 3633
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1126 2005 2497 2795 1126
Size: 879 492 298 2281
Fragments arranged by size:
2281 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 439 3654 439
Size: 3215 735
BsaAI yAC'GTr
Cuts at: 402 1303 402
Size: 901 3049
BsaBI GATnn'nnATC
Cuts at: 6 1464 3501 6
Size: 1458 2037 455
Fragments arranged by size:
2037 1458 455
BsaHI Gr'CG_yC
Cuts at: 171 1381 1629 2331 3827 171
Size: 1210 248 702 1496 294
Fragments arranged by size:
1496 1210 702 294 248
BsaWI w'CCGG_w
Cuts at: 1139 1660 2338 2612 2759 3170 1139
Size: 521 678 274 147 411 1919
Fragments arranged by size:
1919 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 304 334 304
Size: 30 3920
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 157 454 457 157
Size: 297 3 3650
Fragments arranged by size:
3650 297 3
BseYI C'CCAG_C
Cuts at: 272 367 528 598 1375 2133 2710 3206 272
Size: 95 161 70 777 758 577 496 1016
Fragments arranged by size:
1016 777 758 577 496 161 95 70
BsiEI CG_ry'CG
Cuts at: 104 1492 1538 2746 104
Size: 1388 46 1208 1308
Fragments arranged by size:
1388 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 191 820 964 1742 1932 2724 191
Size: 629 144 778 190 792 1417
Fragments arranged by size:
1417 792 778 629 190 144
Bsp1286I G_dGCh'C
Cuts at: 191 537 820 964 1575 1668 1742 1932 1994
Size: 346 283 144 611 93 74 190 62
Cuts at: 1994 2724 191
Size: 730 1417
Fragments arranged by size:
1417 730 611 346 283 190 144 93
74 62
BspEI T'CCGG_A
Cuts at: 3170 3170
Size: 3950
BspHI T'CATG_A
Cuts at: 3126 3126
Size: 3950
BspMI ACCTGCnnnn'nnnn_
Cuts at: 107 652 1000 1214 1516 1897 107
Size: 545 348 214 302 381 2160
Fragments arranged by size:
2160 545 381 348 302 214
BsrBI CCG'CTC
Cuts at: 50 2242 2296 3780 3945 50
Size: 2192 54 1484 165 55
Fragments arranged by size:
2192 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1862 1862
Size: 3950
BsrFI r'CCGG_y
Cuts at: 149 1948 2129 2338 149
Size: 1799 181 209 1761
Fragments arranged by size:
1799 1761 209 181
BssSI C'ACGA_G
Cuts at: 2221 2579 2221
Size: 358 3592
BstAPI GCAn_nnn'nTGC
Cuts at: 1327 1327
Size: 3950
BstBI TT'CG_AA
Cuts at: 2311 2311
Size: 3950
BstXI CCAn_nnnn'nTGG
Cuts at: 165 583 165
Size: 418 3532
BstYI r'GATC_y
Cuts at: 1 975 1442 1465 1800 2046 3058 3144 3156
Size: 974 467 23 335 246 1012 86 12
Cuts at: 3156 3502 1
Size: 346 449
Fragments arranged by size:
1012 974 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 499 499
Size: 3950
BtgI C'CryG_G
Cuts at: 164 164
Size: 3950
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1900 2081 2244 1900
Size: 181 163 3606
Fragments arranged by size:
3606 181 163
BtsI GCAGTG_nn'
Cuts at: 1193 1193
Size: 3950
DraI TTT'AAA
Cuts at: 949 949
Size: 3950
DrdI GACnn_nn'nnGTC
Cuts at: 1656 2514 1656
Size: 858 3092
EaeI y'GGCC_r
Cuts at: 1535 1709 2100 2127 3472 1535
Size: 174 391 27 1345 2013
Fragments arranged by size:
2013 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1535 1535
Size: 3950
EarI CTCTTCn'nnn_
Cuts at: 795 1973 2183 795
Size: 1178 210 2562
Fragments arranged by size:
2562 1178 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 154 190 2158 2468 2614 3760 154
Size: 36 1968 310 146 1146 344
Fragments arranged by size:
1968 1146 344 310 146 36
EcoICRI GAG'CTC
Cuts at: 962 962
Size: 3950
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 568 794 1775 2207 2954 3222 251
Size: 317 226 981 432 747 268 979
Fragments arranged by size:
981 979 747 432 317 268 226
EcoNI CCTnn'n_nnAGG
Cuts at: 454 454
Size: 3950
EcoO109I rG'GnC_Cy
Cuts at: 767 1089 767
Size: 322 3628
EcoRI G'AATT_C
Cuts at: 256 954 256
Size: 698 3252
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1969 1969
Size: 3950
FspI TGC'GCA
Cuts at: 1731 1731
Size: 3950
HaeII r_GCGC'y
Cuts at: 156 174 374 1384 1632 2654 3633 156
Size: 18 200 1010 248 1022 979 473
Fragments arranged by size:
1022 1010 979 473 248 200 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1792 1822 1824 1854 1792
Size: 30 2 30 3888
Fragments arranged by size:
3888 30 30 2
HincII GTy'rAC
Cuts at: 989 989
Size: 3950
HindIII A'AGCT_T
Cuts at: 900 900
Size: 3950
KasI G'GCGC_C
Cuts at: 170 1380 1628 170
Size: 1210 248 2492
Fragments arranged by size:
2492 1210 248
KpnI G_GTAC'C
Cuts at: 970 970
Size: 3950
MluI A'CGCG_T
Cuts at: 3383 3383
Size: 3950
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 909 2621 2805 909
Size: 1712 184 2054
Fragments arranged by size:
2054 1712 184
MscI TGG'CCA
Cuts at: 1711 1711
Size: 3950
MslI CAynn'nnrTG
Cuts at: 163 355 581 1939 2066 163
Size: 192 226 1358 127 2047
Fragments arranged by size:
2047 1358 226 192 127
MspA1I CmG'CkG
Cuts at: 327 528 704 1061 1375 1735 2748 2993 327
Size: 201 176 357 314 360 1013 245 1284
Fragments arranged by size:
1284 1013 360 357 314 245 201 176
NaeI GCC'GGC
Cuts at: 151 2131 151
Size: 1980 1970
NarI GG'CG_CC
Cuts at: 171 1381 1629 171
Size: 1210 248 2492
Fragments arranged by size:
2492 1210 248
NgoMIV G'CCGG_C
Cuts at: 149 2129 149
Size: 1980 1970
NspI r_CATG'y
Cuts at: 614 1003 1503 2410 614
Size: 389 500 907 2154
Fragments arranged by size:
2154 907 500 389
PasI CC'CwG_GG
Cuts at: 484 561 484
Size: 77 3873
PciI A'CATG_T
Cuts at: 2406 2406
Size: 3950
PflMI CCAn_nnn'nTGG
Cuts at: 490 490
Size: 3950
PfoI T'CCnGG_A
Cuts at: 545 891 545
Size: 346 3604
PmeI GTTT'AAAC
Cuts at: 949 949
Size: 3950
PmlI CAC'GTG
Cuts at: 402 402
Size: 3950
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3089 3121 3089
Size: 32 3918
PpuMI rG'GwC_Cy
Cuts at: 767 767
Size: 3950
PstI C_TGCA'G
Cuts at: 997 1682 997
Size: 685 3265
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3950
PvuII CAG'CTG
Cuts at: 528 1375 1735 528
Size: 847 360 2743
Fragments arranged by size:
2743 847 360
RsrII CG'GwC_CG
Cuts at: 2145 2145
Size: 3950
SacI G_AGCT'C
Cuts at: 964 964
Size: 3950
SalI G'TCGA_C
Cuts at: 987 987
Size: 3950
SapI GCTCTTCn'nnn_
Cuts at: 1973 2183 1973
Size: 210 3740
SbfI CC_TGCA'GG
Cuts at: 997 997
Size: 3950
ScaI AGT'ACT
Cuts at: 3467 3467
Size: 3950
SfcI C'TryA_G
Cuts at: 33 993 1678 2671 2862 33
Size: 960 685 993 191 1121
Fragments arranged by size:
1121 993 960 685 191
SfoI GGC'GCC
Cuts at: 172 1382 1630 172
Size: 1210 248 2492
Fragments arranged by size:
2492 1210 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3950
SmaI CCC'GGG
Cuts at: 972 972
Size: 3950
SmlI C'TyrA_G
Cuts at: 1105 2020 2512 2774 1105
Size: 915 492 262 2281
Fragments arranged by size:
2281 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3950
SphI G_CATG'C
Cuts at: 1003 1503 1003
Size: 500 3450
StyI C'CwwG_G
Cuts at: 630 1084 1438 630
Size: 454 354 3142
Fragments arranged by size:
3142 454 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 286 1533 1666 2125 2334 286
Size: 1247 133 459 209 1902
Fragments arranged by size:
1902 1247 459 209 133
TatI w'GTAC_w
Cuts at: 3465 3465
Size: 3950
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 304 336 769 801 3467 3499 304
Size: 32 433 32 2666 32 755
Fragments arranged by size:
2666 755 433 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1747 1747
Size: 3950
XbaI T'CTAG_A
Cuts at: 981 981
Size: 3950
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1445 1445
Size: 3950
XmaI C'CCGG_G
Cuts at: 970 970
Size: 3950
XmnI GAAnn'nnTTC
Cuts at: 887 887
Size: 3950
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI