Restriction map for
pF1KE0578
(Circular) (Six-base) MAPSORT of: pF1KE0578.seq Check: 1076 from: 1 to: 3734
pF1KE0578 3734 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 772 772
Size: 3734
Acc65I G'GTAC_C
Cuts at: 750 750
Size: 3734
AclI AA'CG_TT
Cuts at: 3574 3574
Size: 3734
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 1559 1991 2738 3006 295
Size: 1264 432 747 268 1023
Fragments arranged by size:
1264 1023 747 432 268
AflIII A'CryG_T
Cuts at: 496 1086 2190 3167 496
Size: 590 1104 977 1063
Fragments arranged by size:
1104 1063 977 590
AgeI A'CCGG_T
Cuts at: 2122 2122
Size: 3734
AlwNI CAG_nnn'CTG
Cuts at: 1039 2606 1039
Size: 1567 2167
ApaLI G'TGCA_C
Cuts at: 349 2504 349
Size: 2155 1579
ApoI r'AATT_y
Cuts at: 652 738 3087 3343 652
Size: 86 2349 256 1043
Fragments arranged by size:
2349 1043 256 86
AseI AT'TA_AT
Cuts at: 20 20
Size: 3734
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3734
AvaI C'yCGr_G
Cuts at: 620 754 620
Size: 134 3600
AvrII C'CTAG_G
Cuts at: 450 450
Size: 3734
BamHI G'GATC_C
Cuts at: 281 759 281
Size: 478 3256
BanI G'GyrC_C
Cuts at: 290 750 1164 1412 1447 290
Size: 460 414 248 35 2577
Fragments arranged by size:
2577 460 414 248 35
BanII G_rGCy'C
Cuts at: 748 1778 748
Size: 1030 2704
BbeI G_GCGC'C
Cuts at: 294 1168 1416 294
Size: 874 248 2612
Fragments arranged by size:
2612 874 248
BbsI GAAGACnn'nnnn_
Cuts at: 120 120
Size: 3734
BciVI GTATCCnnnnn_n'
Cuts at: 622 1632 2393 3734 622
Size: 1010 761 1341 622
Fragments arranged by size:
1341 1010 761 622
BclI T'GATC_A
Cuts at: 416 1254 416
Size: 838 2896
BglI GCCn_nnn'nGGC
Cuts at: 314 314
Size: 3734
BglII A'GATC_T
Cuts at: 329 1249 3286 329
Size: 920 2037 777
Fragments arranged by size:
2037 920 777
BlpI GC'TnA_GC
Cuts at: 846 846
Size: 3734
Bme1580I G_kGCm'C
Cuts at: 185 353 1359 1452 2508 185
Size: 168 1006 93 1056 1411
Fragments arranged by size:
1411 1056 1006 168 93
BmrI ACTGGGnnnn_n'
Cuts at: 153 946 1027 1362 3506 153
Size: 793 81 335 2144 381
Fragments arranged by size:
2144 793 381 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 490 2101 490
Size: 1611 2123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 409 346
Size: 63 3671
Bpu10I CC'TnA_GC
Cuts at: 186 186
Size: 3734
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 910 1789 2281 2579 910
Size: 879 492 298 2065
Fragments arranged by size:
2065 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 704 3438 704
Size: 2734 1000
BsaAI yAC'GTr
Cuts at: 499 594 1087 499
Size: 95 493 3146
Fragments arranged by size:
3146 493 95
BsaBI GATnn'nnATC
Cuts at: 6 421 1248 3285 6
Size: 415 827 2037 455
Fragments arranged by size:
2037 827 455 415
BsaHI Gr'CG_yC
Cuts at: 125 291 1165 1413 2115 3611 125
Size: 166 874 248 702 1496 248
Fragments arranged by size:
1496 874 702 248 248 166
BsaWI w'CCGG_w
Cuts at: 923 1444 2122 2396 2543 2954 923
Size: 521 678 274 147 411 1703
Fragments arranged by size:
1703 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 145 175 145
Size: 30 3704
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 153 482 153
Size: 329 3405
BseYI C'CCAG_C
Cuts at: 1159 1917 2494 2990 1159
Size: 758 577 496 1903
Fragments arranged by size:
1903 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1276 1322 2530 104
Size: 1172 46 1208 1308
Fragments arranged by size:
1308 1208 1172 46
BsiHKAI G_wGCw'C
Cuts at: 353 748 1526 1716 2508 353
Size: 395 778 190 792 1579
Fragments arranged by size:
1579 792 778 395 190
BsmBI CGTCTCn'nnnn_
Cuts at: 173 173
Size: 3734
Bsp1286I G_dGCh'C
Cuts at: 185 353 748 1359 1452 1526 1716 1778 2508
Size: 168 395 611 93 74 190 62 730
Cuts at: 2508 185
Size: 1411
Fragments arranged by size:
1411 730 611 395 190 168 93 74
62
BspEI T'CCGG_A
Cuts at: 2954 2954
Size: 3734
BspHI T'CATG_A
Cuts at: 2910 2910
Size: 3734
BspMI ACCTGCnnnn'nnnn_
Cuts at: 784 998 1300 1681 784
Size: 214 302 381 2837
Fragments arranged by size:
2837 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2026 2080 3564 3729 50
Size: 1976 54 1484 165 55
Fragments arranged by size:
1976 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1646 1646
Size: 3734
BsrFI r'CCGG_y
Cuts at: 313 1732 1913 2122 313
Size: 1419 181 209 1925
Fragments arranged by size:
1925 1419 209 181
BsrGI T'GTAC_A
Cuts at: 560 560
Size: 3734
BssSI C'ACGA_G
Cuts at: 2005 2363 2005
Size: 358 3376
BstAPI GCAn_nnn'nTGC
Cuts at: 1111 1111
Size: 3734
BstBI TT'CG_AA
Cuts at: 2095 2095
Size: 3734
BstYI r'GATC_y
Cuts at: 1 147 281 329 681 759 1226 1249 1584
Size: 146 134 48 352 78 467 23 335
Cuts at: 1584 1830 2842 2928 2940 3286 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 352 346 335 246 146
134 86 78 48 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3734
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1684 1865 2028 1684
Size: 181 163 3390
Fragments arranged by size:
3390 181 163
BtsI GCAGTG_nn'
Cuts at: 977 977
Size: 3734
DraI TTT'AAA
Cuts at: 733 733
Size: 3734
DrdI GACnn_nn'nnGTC
Cuts at: 1440 2298 1440
Size: 858 2876
EaeI y'GGCC_r
Cuts at: 1319 1493 1884 1911 3256 1319
Size: 174 391 27 1345 1797
Fragments arranged by size:
1797 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1319 1319
Size: 3734
EarI CTCTTCn'nnn_
Cuts at: 1757 1967 1757
Size: 210 3524
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1942 2252 2398 3544 1942
Size: 310 146 1146 2132
Fragments arranged by size:
2132 1146 310 146
EcoICRI GAG'CTC
Cuts at: 746 746
Size: 3734
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 346 409 1559 1991 2738 3006 295
Size: 51 63 1150 432 747 268 1023
Fragments arranged by size:
1150 1023 747 432 268 63 51
EcoO109I rG'GnC_Cy
Cuts at: 356 873 356
Size: 517 3217
EcoRI G'AATT_C
Cuts at: 738 738
Size: 3734
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1753 1753
Size: 3734
FspI TGC'GCA
Cuts at: 1515 1515
Size: 3734
HaeII r_GCGC'y
Cuts at: 219 294 1168 1416 2438 3417 219
Size: 75 874 248 1022 979 536
Fragments arranged by size:
1022 979 874 536 248 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1576 1606 1608 1638 1576
Size: 30 2 30 3672
Fragments arranged by size:
3672 30 30 2
HincII GTy'rAC
Cuts at: 773 773
Size: 3734
KasI G'GCGC_C
Cuts at: 290 1164 1412 290
Size: 874 248 2612
Fragments arranged by size:
2612 874 248
KpnI G_GTAC'C
Cuts at: 754 754
Size: 3734
MluI A'CGCG_T
Cuts at: 3167 3167
Size: 3734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2405 2589 2405
Size: 184 3550
MscI TGG'CCA
Cuts at: 1495 1495
Size: 3734
MslI CAynn'nnrTG
Cuts at: 501 1723 1850 501
Size: 1222 127 2385
Fragments arranged by size:
2385 1222 127
MspA1I CmG'CkG
Cuts at: 845 1159 1519 2532 2777 845
Size: 314 360 1013 245 1802
Fragments arranged by size:
1802 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1915 1915
Size: 3734
NarI GG'CG_CC
Cuts at: 291 1165 1413 291
Size: 874 248 2612
Fragments arranged by size:
2612 874 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3734
NgoMIV G'CCGG_C
Cuts at: 1913 1913
Size: 3734
NspI r_CATG'y
Cuts at: 787 1287 2194 787
Size: 500 907 2327
Fragments arranged by size:
2327 907 500
PciI A'CATG_T
Cuts at: 2190 2190
Size: 3734
PfoI T'CCnGG_A
Cuts at: 386 512 386
Size: 126 3608
PmeI GTTT'AAAC
Cuts at: 733 733
Size: 3734
PmlI CAC'GTG
Cuts at: 499 499
Size: 3734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2873 2905 2873
Size: 32 3702
PpuMI rG'GwC_Cy
Cuts at: 356 356
Size: 3734
PstI C_TGCA'G
Cuts at: 245 781 1466 245
Size: 536 685 2513
Fragments arranged by size:
2513 685 536
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3734
PvuII CAG'CTG
Cuts at: 1159 1519 1159
Size: 360 3374
RsrII CG'GwC_CG
Cuts at: 1929 1929
Size: 3734
SacI G_AGCT'C
Cuts at: 748 748
Size: 3734
SalI G'TCGA_C
Cuts at: 771 771
Size: 3734
SanDI GG'GwC_CC
Cuts at: 356 356
Size: 3734
SapI GCTCTTCn'nnn_
Cuts at: 1757 1967 1757
Size: 210 3524
SbfI CC_TGCA'GG
Cuts at: 781 781
Size: 3734
ScaI AGT'ACT
Cuts at: 3251 3251
Size: 3734
SfcI C'TryA_G
Cuts at: 33 241 677 723 777 1462 2455 2646 33
Size: 208 436 46 54 685 993 191 1121
Fragments arranged by size:
1121 993 685 436 208 191 54 46
SfoI GGC'GCC
Cuts at: 292 1166 1414 292
Size: 874 248 2612
Fragments arranged by size:
2612 874 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3734
SmaI CCC'GGG
Cuts at: 622 756 622
Size: 134 3600
SmlI C'TyrA_G
Cuts at: 889 1804 2296 2558 889
Size: 915 492 262 2065
Fragments arranged by size:
2065 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3734
SphI G_CATG'C
Cuts at: 787 1287 787
Size: 500 3234
StuI AGG'CCT
Cuts at: 675 675
Size: 3734
StyI C'CwwG_G
Cuts at: 107 137 450 868 1222 107
Size: 30 313 418 354 2619
Fragments arranged by size:
2619 418 354 313 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 270 1317 1450 1909 2118 270
Size: 1047 133 459 209 1886
Fragments arranged by size:
1886 1047 459 209 133
TatI w'GTAC_w
Cuts at: 560 3249 560
Size: 2689 1045
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 145 177 3251 3283 145
Size: 32 3074 32 596
Fragments arranged by size:
3074 596 32 32
Tth111I GACn'n_nGTC
Cuts at: 1531 1531
Size: 3734
XbaI T'CTAG_A
Cuts at: 765 765
Size: 3734
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1229 114
Size: 1115 2619
XmaI C'CCGG_G
Cuts at: 620 754 620
Size: 134 3600
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PmlI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BbvCI BcgI BfrBI BmgBI BmtI
BsgI BsiWI BsmI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PshAI PsiI PspOMI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI