Restriction map for
pF1KE0577
(Circular) (Six-base) MAPSORT of: pF1KE0577.seq Check: 1648 from: 1 to: 4040
pF1KE0577 4040 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 1078 1078
Size: 4040
Acc65I G'GTAC_C
Cuts at: 1056 1056
Size: 4040
AclI AA'CG_TT
Cuts at: 3880 3880
Size: 4040
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 935 1865 2297 3044 3312 935
Size: 930 432 747 268 1663
Fragments arranged by size:
1663 930 747 432 268
AfeI AGC'GCT
Cuts at: 537 537
Size: 4040
AflIII A'CryG_T
Cuts at: 1392 2496 3473 1392
Size: 1104 977 1959
Fragments arranged by size:
1959 1104 977
AgeI A'CCGG_T
Cuts at: 2428 2428
Size: 4040
AleI CACnn'nnGTG
Cuts at: 229 229
Size: 4040
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 236 268 236
Size: 32 4008
AlwNI CAG_nnn'CTG
Cuts at: 1345 2912 1345
Size: 1567 2473
ApaLI G'TGCA_C
Cuts at: 2810 2810
Size: 4040
ApoI r'AATT_y
Cuts at: 653 671 1044 3393 3649 653
Size: 18 373 2349 256 1044
Fragments arranged by size:
2349 1044 373 256 18
AseI AT'TA_AT
Cuts at: 20 20
Size: 4040
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
AvaI C'yCGr_G
Cuts at: 1060 1060
Size: 4040
AvrII C'CTAG_G
Cuts at: 349 349
Size: 4040
BamHI G'GATC_C
Cuts at: 1065 1065
Size: 4040
BanI G'GyrC_C
Cuts at: 838 1056 1470 1718 1753 838
Size: 218 414 248 35 3125
Fragments arranged by size:
3125 414 248 218 35
BanII G_rGCy'C
Cuts at: 1054 2084 1054
Size: 1030 3010
BbeI G_GCGC'C
Cuts at: 842 1474 1722 842
Size: 632 248 3160
Fragments arranged by size:
3160 632 248
BbsI GAAGACnn'nnnn_
Cuts at: 923 923
Size: 4040
BciVI GTATCCnnnnn_n'
Cuts at: 1938 2699 4040 1938
Size: 761 1341 1938
Fragments arranged by size:
1938 1341 761
BclI T'GATC_A
Cuts at: 1560 1560
Size: 4040
BfrBI ATG'CAT
Cuts at: 1008 1012 1008
Size: 4 4036
BglII A'GATC_T
Cuts at: 1555 3592 1555
Size: 2037 2003
BlpI GC'TnA_GC
Cuts at: 909 1152 909
Size: 243 3797
Bme1580I G_kGCm'C
Cuts at: 1665 1758 2814 1665
Size: 93 1056 2891
Fragments arranged by size:
2891 1056 93
BmgBI CAC'GTC
Cuts at: 186 186
Size: 4040
BmrI ACTGGGnnnn_n'
Cuts at: 405 1252 1333 1668 3812 405
Size: 847 81 335 2144 633
Fragments arranged by size:
2144 847 633 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 4040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1008 1216 2095 2587 2885 1008
Size: 208 879 492 298 2163
Fragments arranged by size:
2163 879 492 298 208
BsaI GGTCTCn'nnnn_
Cuts at: 3744 3744
Size: 4040
BsaAI yAC'GTr
Cuts at: 1393 1393
Size: 4040
BsaBI GATnn'nnATC
Cuts at: 6 1554 3591 6
Size: 1548 2037 455
Fragments arranged by size:
2037 1548 455
BsaHI Gr'CG_yC
Cuts at: 839 1471 1719 2421 3917 839
Size: 632 248 702 1496 962
Fragments arranged by size:
1496 962 702 632 248
BsaWI w'CCGG_w
Cuts at: 1229 1750 2428 2702 2849 3260 1229
Size: 521 678 274 147 411 2009
Fragments arranged by size:
2009 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1465 2223 2800 3296 1465
Size: 758 577 496 2209
Fragments arranged by size:
2209 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 166
Size: 4040
BsiEI CG_ry'CG
Cuts at: 104 1582 1628 2836 104
Size: 1478 46 1208 1308
Fragments arranged by size:
1478 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 779 1054 1832 2022 2814 779
Size: 275 778 190 792 2005
Fragments arranged by size:
2005 792 778 275 190
BsmI GAATG_Cn'
Cuts at: 451 451
Size: 4040
Bsp1286I G_dGCh'C
Cuts at: 779 1054 1665 1758 1832 2022 2084 2814 779
Size: 275 611 93 74 190 62 730 2005
Fragments arranged by size:
2005 730 611 275 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3260 3260
Size: 4040
BspHI T'CATG_A
Cuts at: 3216 3216
Size: 4040
BspMI ACCTGCnnnn'nnnn_
Cuts at: 611 1090 1304 1606 1987 611
Size: 479 214 302 381 2664
Fragments arranged by size:
2664 479 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2332 2386 3870 4035 50
Size: 2282 54 1484 165 55
Fragments arranged by size:
2282 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 389 687 1952 389
Size: 298 1265 2477
Fragments arranged by size:
2477 1265 298
BsrFI r'CCGG_y
Cuts at: 2038 2219 2428 2038
Size: 181 209 3650
Fragments arranged by size:
3650 209 181
BssSI C'ACGA_G
Cuts at: 770 2311 2669 770
Size: 1541 358 2141
Fragments arranged by size:
2141 1541 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1417 1417
Size: 4040
BstBI TT'CG_AA
Cuts at: 2401 2401
Size: 4040
BstYI r'GATC_y
Cuts at: 1 1065 1532 1555 1890 2136 3148 3234 3246
Size: 1064 467 23 335 246 1012 86 12
Cuts at: 3246 3592 1
Size: 346 449
Fragments arranged by size:
1064 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 175 227 107
Size: 68 52 3920
Fragments arranged by size:
3920 68 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1990 2171 2334 1990
Size: 181 163 3696
Fragments arranged by size:
3696 181 163
BtsI GCAGTG_nn'
Cuts at: 1283 1283
Size: 4040
DraI TTT'AAA
Cuts at: 1039 1039
Size: 4040
DrdI GACnn_nn'nnGTC
Cuts at: 1746 2604 1746
Size: 858 3182
EaeI y'GGCC_r
Cuts at: 1625 1799 2190 2217 3562 1625
Size: 174 391 27 1345 2103
Fragments arranged by size:
2103 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1625 1625
Size: 4040
EarI CTCTTCn'nnn_
Cuts at: 196 1014 2063 2273 196
Size: 818 1049 210 1963
Fragments arranged by size:
1963 1049 818 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2248 2558 2704 3850 2248
Size: 310 146 1146 2438
Fragments arranged by size:
2438 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1052 1052
Size: 4040
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 935 1865 2297 3044 3312 935
Size: 930 432 747 268 1663
Fragments arranged by size:
1663 930 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1179 1179
Size: 4040
EcoRI G'AATT_C
Cuts at: 1044 1044
Size: 4040
EcoRV GAT'ATC
Cuts at: 389 389
Size: 4040
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2059 2059
Size: 4040
FspI TGC'GCA
Cuts at: 1821 1821
Size: 4040
HaeII r_GCGC'y
Cuts at: 539 842 1474 1722 2744 3723 539
Size: 303 632 248 1022 979 856
Fragments arranged by size:
1022 979 856 632 303 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1882 1912 1914 1944 1882
Size: 30 2 30 3978
Fragments arranged by size:
3978 30 30 2
HincII GTy'rAC
Cuts at: 1079 1079
Size: 4040
KasI G'GCGC_C
Cuts at: 838 1470 1718 838
Size: 632 248 3160
Fragments arranged by size:
3160 632 248
KpnI G_GTAC'C
Cuts at: 1060 1060
Size: 4040
MluI A'CGCG_T
Cuts at: 3473 3473
Size: 4040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 548 2711 2895 548
Size: 2163 184 1693
Fragments arranged by size:
2163 1693 184
MscI TGG'CCA
Cuts at: 1801 1801
Size: 4040
MslI CAynn'nnrTG
Cuts at: 226 229 745 889 2029 2156 226
Size: 3 516 144 1140 127 2110
Fragments arranged by size:
2110 1140 516 144 127 3
MspA1I CmG'CkG
Cuts at: 1151 1465 1825 2838 3083 1151
Size: 314 360 1013 245 2108
Fragments arranged by size:
2108 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2221 2221
Size: 4040
NarI GG'CG_CC
Cuts at: 839 1471 1719 839
Size: 632 248 3160
Fragments arranged by size:
3160 632 248
NcoI C'CATG_G
Cuts at: 107 175 227 107
Size: 68 52 3920
Fragments arranged by size:
3920 68 52
NdeI CA'TA_TG
Cuts at: 281 490 558 281
Size: 209 68 3763
Fragments arranged by size:
3763 209 68
NgoMIV G'CCGG_C
Cuts at: 2219 2219
Size: 4040
NsiI A_TGCA'T
Cuts at: 1010 1014 1010
Size: 4 4036
NspI r_CATG'y
Cuts at: 836 1012 1093 1593 2500 836
Size: 176 81 500 907 2376
Fragments arranged by size:
2376 907 500 176 81
PciI A'CATG_T
Cuts at: 2496 2496
Size: 4040
PmeI GTTT'AAAC
Cuts at: 1039 1039
Size: 4040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 4008
PsiI TTA'TAA
Cuts at: 955 1027 955
Size: 72 3968
PstI C_TGCA'G
Cuts at: 622 1087 1772 622
Size: 465 685 2890
Fragments arranged by size:
2890 685 465
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4040
PvuII CAG'CTG
Cuts at: 1465 1825 1465
Size: 360 3680
RsrII CG'GwC_CG
Cuts at: 2235 2235
Size: 4040
SacI G_AGCT'C
Cuts at: 1054 1054
Size: 4040
SalI G'TCGA_C
Cuts at: 1077 1077
Size: 4040
SapI GCTCTTCn'nnn_
Cuts at: 2063 2273 2063
Size: 210 3830
SbfI CC_TGCA'GG
Cuts at: 1087 1087
Size: 4040
ScaI AGT'ACT
Cuts at: 3557 3557
Size: 4040
SfcI C'TryA_G
Cuts at: 33 440 618 1083 1768 2761 2952 33
Size: 407 178 465 685 993 191 1121
Fragments arranged by size:
1121 993 685 465 407 191 178
SfoI GGC'GCC
Cuts at: 840 1472 1720 840
Size: 632 248 3160
Fragments arranged by size:
3160 632 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
SmaI CCC'GGG
Cuts at: 1062 1062
Size: 4040
SmlI C'TyrA_G
Cuts at: 987 1195 2110 2602 2864 987
Size: 208 915 492 262 2163
Fragments arranged by size:
2163 915 492 262 208
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4040
SphI G_CATG'C
Cuts at: 1012 1093 1593 1012
Size: 81 500 3459
Fragments arranged by size:
3459 500 81
StyI C'CwwG_G
Cuts at: 107 175 227 240 300 349 1174 1528 107
Size: 68 52 13 60 49 825 354 2619
Fragments arranged by size:
2619 825 354 68 60 52 49 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 424 1623 1756 2215 2424 424
Size: 1199 133 459 209 2040
Fragments arranged by size:
2040 1199 459 209 133
TatI w'GTAC_w
Cuts at: 290 328 863 3555 290
Size: 38 535 2692 775
Fragments arranged by size:
2692 775 535 38
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3557 3589 3557
Size: 32 4008
Tth111I GACn'n_nGTC
Cuts at: 1837 1837
Size: 4040
XbaI T'CTAG_A
Cuts at: 1071 1071
Size: 4040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1535 1535
Size: 4040
XmaI C'CCGG_G
Cuts at: 1060 1060
Size: 4040
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PciI PmeI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI ApaI AscI BaeI
BbvCI BcgI BglI BmtI BpmI Bpu10I BsaXI BseRI
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI FseI FspAI HindIII HpaI
MfeI NheI NotI NruI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI