Restriction map for
pF1KE0576
(Circular) (Six-base) MAPSORT of: pF1KE0576.seq Check: 8780 from: 1 to: 3926
pF1KE0576 3926 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 964 964
Size: 3926
Acc65I G'GTAC_C
Cuts at: 942 942
Size: 3926
AclI AA'CG_TT
Cuts at: 3766 3766
Size: 3926
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1751 2183 2930 3198 1751
Size: 432 747 268 2479
Fragments arranged by size:
2479 747 432 268
AflIII A'CryG_T
Cuts at: 1278 2382 3359 1278
Size: 1104 977 1845
Fragments arranged by size:
1845 1104 977
AgeI A'CCGG_T
Cuts at: 2314 2314
Size: 3926
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 366 397 366
Size: 31 3895
AlwNI CAG_nnn'CTG
Cuts at: 1231 2798 1231
Size: 1567 2359
ApaI G_GGCC'C
Cuts at: 573 573
Size: 3926
ApaLI G'TGCA_C
Cuts at: 2696 2696
Size: 3926
ApoI r'AATT_y
Cuts at: 460 868 930 3279 3535 460
Size: 408 62 2349 256 851
Fragments arranged by size:
2349 851 408 256 62
AseI AT'TA_AT
Cuts at: 20 20
Size: 3926
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3926
AvaI C'yCGr_G
Cuts at: 946 946
Size: 3926
BamHI G'GATC_C
Cuts at: 951 951
Size: 3926
BanI G'GyrC_C
Cuts at: 942 1356 1604 1639 942
Size: 414 248 35 3229
Fragments arranged by size:
3229 414 248 35
BanII G_rGCy'C
Cuts at: 573 940 1970 573
Size: 367 1030 2529
Fragments arranged by size:
2529 1030 367
BbeI G_GCGC'C
Cuts at: 1360 1608 1360
Size: 248 3678
BciVI GTATCCnnnnn_n'
Cuts at: 1824 2585 3926 1824
Size: 761 1341 1824
Fragments arranged by size:
1824 1341 761
BclI T'GATC_A
Cuts at: 1446 1446
Size: 3926
BfrBI ATG'CAT
Cuts at: 886 886
Size: 3926
BglII A'GATC_T
Cuts at: 1441 3478 1441
Size: 2037 1889
BlpI GC'TnA_GC
Cuts at: 470 1038 470
Size: 568 3358
Bme1580I G_kGCm'C
Cuts at: 573 1551 1644 2700 573
Size: 978 93 1056 1799
Fragments arranged by size:
1799 1056 978 93
BmrI ACTGGGnnnn_n'
Cuts at: 1138 1219 1554 3698 1138
Size: 81 335 2144 1366
Fragments arranged by size:
2144 1366 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2293 2293
Size: 3926
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1102 1981 2473 2771 1102
Size: 879 492 298 2257
Fragments arranged by size:
2257 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 730 3630 730
Size: 2900 1026
BsaAI yAC'GTr
Cuts at: 1279 1279
Size: 3926
BsaBI GATnn'nnATC
Cuts at: 6 1440 3477 6
Size: 1434 2037 455
Fragments arranged by size:
2037 1434 455
BsaHI Gr'CG_yC
Cuts at: 1357 1605 2307 3803 1357
Size: 248 702 1496 1480
Fragments arranged by size:
1496 1480 702 248
BsaWI w'CCGG_w
Cuts at: 326 1115 1636 2314 2588 2735 3146 326
Size: 789 521 678 274 147 411 1106
Fragments arranged by size:
1106 789 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 127 157 127
Size: 30 3896
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 538 538
Size: 3926
BseYI C'CCAG_C
Cuts at: 673 818 1351 2109 2686 3182 673
Size: 145 533 758 577 496 1417
Fragments arranged by size:
1417 758 577 533 496 145
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 627 877 627
Size: 250 3676
BsiEI CG_ry'CG
Cuts at: 104 1468 1514 2722 104
Size: 1364 46 1208 1308
Fragments arranged by size:
1364 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 476 548 940 1718 1908 2700 476
Size: 72 392 778 190 792 1702
Fragments arranged by size:
1702 792 778 392 190 72
Bsp1286I G_dGCh'C
Cuts at: 476 548 573 940 1551 1644 1718 1908 1970
Size: 72 25 367 611 93 74 190 62
Cuts at: 1970 2700 476
Size: 730 1702
Fragments arranged by size:
1702 730 611 367 190 93 74 72
62 25
BspEI T'CCGG_A
Cuts at: 3146 3146
Size: 3926
BspHI T'CATG_A
Cuts at: 3102 3102
Size: 3926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 976 1190 1492 1873 976
Size: 214 302 381 3029
Fragments arranged by size:
3029 381 302 214
BsrBI CCG'CTC
Cuts at: 50 138 242 2218 2272 3756 3921 50
Size: 88 104 1976 54 1484 165 55
Fragments arranged by size:
1976 1484 165 104 88 55 54
BsrDI GCAATG_nn'
Cuts at: 669 843 1838 669
Size: 174 995 2757
Fragments arranged by size:
2757 995 174
BsrFI r'CCGG_y
Cuts at: 1924 2105 2314 1924
Size: 181 209 3536
Fragments arranged by size:
3536 209 181
BssSI C'ACGA_G
Cuts at: 2197 2555 2197
Size: 358 3568
BstAPI GCAn_nnn'nTGC
Cuts at: 718 1303 718
Size: 585 3341
BstBI TT'CG_AA
Cuts at: 2287 2287
Size: 3926
BstEII G'GTnAC_C
Cuts at: 453 453
Size: 3926
BstXI CCAn_nnnn'nTGG
Cuts at: 465 465
Size: 3926
BstYI r'GATC_y
Cuts at: 1 656 951 1418 1441 1776 2022 3034 3120
Size: 655 295 467 23 335 246 1012 86
Cuts at: 3120 3132 3478 1
Size: 12 346 449
Fragments arranged by size:
1012 655 467 449 346 335 295 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 550 550
Size: 3926
BtgI C'CryG_G
Cuts at: 668 668
Size: 3926
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1876 2057 2220 1876
Size: 181 163 3582
Fragments arranged by size:
3582 181 163
BtsI GCAGTG_nn'
Cuts at: 1169 1169
Size: 3926
DraI TTT'AAA
Cuts at: 925 925
Size: 3926
DrdI GACnn_nn'nnGTC
Cuts at: 265 1632 2490 265
Size: 1367 858 1701
Fragments arranged by size:
1701 1367 858
EaeI y'GGCC_r
Cuts at: 1511 1685 2076 2103 3448 1511
Size: 174 391 27 1345 1989
Fragments arranged by size:
1989 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1511 1511
Size: 3926
EarI CTCTTCn'nnn_
Cuts at: 799 1949 2159 799
Size: 1150 210 2566
Fragments arranged by size:
2566 1150 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2134 2444 2590 3736 2134
Size: 310 146 1146 2324
Fragments arranged by size:
2324 1146 310 146
EcoICRI GAG'CTC
Cuts at: 938 938
Size: 3926
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1751 2183 2930 3198 1751
Size: 432 747 268 2479
Fragments arranged by size:
2479 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 569 1065 569
Size: 496 3430
EcoRI G'AATT_C
Cuts at: 930 930
Size: 3926
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1945 1945
Size: 3926
FspI TGC'GCA
Cuts at: 1707 1707
Size: 3926
HaeII r_GCGC'y
Cuts at: 399 1360 1608 2630 3609 399
Size: 961 248 1022 979 716
Fragments arranged by size:
1022 979 961 716 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 111 143 1768 1798 1800 1830 111
Size: 32 1625 30 2 30 2207
Fragments arranged by size:
2207 1625 32 30 30 2
HincII GTy'rAC
Cuts at: 965 965
Size: 3926
KasI G'GCGC_C
Cuts at: 1356 1604 1356
Size: 248 3678
KpnI G_GTAC'C
Cuts at: 946 946
Size: 3926
MluI A'CGCG_T
Cuts at: 3359 3359
Size: 3926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 175 2597 2781 175
Size: 2422 184 1320
Fragments arranged by size:
2422 1320 184
MscI TGG'CCA
Cuts at: 1687 1687
Size: 3926
MslI CAynn'nnrTG
Cuts at: 479 1915 2042 479
Size: 1436 127 2363
Fragments arranged by size:
2363 1436 127
MspA1I CmG'CkG
Cuts at: 1037 1351 1711 2724 2969 1037
Size: 314 360 1013 245 1994
Fragments arranged by size:
1994 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2107 2107
Size: 3926
NarI GG'CG_CC
Cuts at: 1357 1605 1357
Size: 248 3678
NcoI C'CATG_G
Cuts at: 668 668
Size: 3926
NgoMIV G'CCGG_C
Cuts at: 2105 2105
Size: 3926
NsiI A_TGCA'T
Cuts at: 888 888
Size: 3926
NspI r_CATG'y
Cuts at: 979 1479 2386 979
Size: 500 907 2519
Fragments arranged by size:
2519 907 500
PasI CC'CwG_GG
Cuts at: 760 760
Size: 3926
PciI A'CATG_T
Cuts at: 2382 2382
Size: 3926
PmeI GTTT'AAAC
Cuts at: 925 925
Size: 3926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 170 202 3065 3097 170
Size: 32 2863 32 999
Fragments arranged by size:
2863 999 32 32
PspOMI G'GGCC_C
Cuts at: 569 569
Size: 3926
PstI C_TGCA'G
Cuts at: 699 973 1658 699
Size: 274 685 2967
Fragments arranged by size:
2967 685 274
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3926
PvuII CAG'CTG
Cuts at: 1351 1711 1351
Size: 360 3566
RsrII CG'GwC_CG
Cuts at: 2121 2121
Size: 3926
SacI G_AGCT'C
Cuts at: 940 940
Size: 3926
SalI G'TCGA_C
Cuts at: 963 963
Size: 3926
SapI GCTCTTCn'nnn_
Cuts at: 1949 2159 1949
Size: 210 3716
SbfI CC_TGCA'GG
Cuts at: 699 973 699
Size: 274 3652
ScaI AGT'ACT
Cuts at: 3443 3443
Size: 3926
SexAI A'CCwGG_T
Cuts at: 409 409
Size: 3926
SfcI C'TryA_G
Cuts at: 33 695 969 1654 2647 2838 33
Size: 662 274 685 993 191 1121
Fragments arranged by size:
1121 993 685 662 274 191
SfoI GGC'GCC
Cuts at: 1358 1606 1358
Size: 248 3678
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3926
SmaI CCC'GGG
Cuts at: 948 948
Size: 3926
SmlI C'TyrA_G
Cuts at: 1081 1996 2488 2750 1081
Size: 915 492 262 2257
Fragments arranged by size:
2257 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3926
SphI G_CATG'C
Cuts at: 979 1479 979
Size: 500 3426
StuI AGG'CCT
Cuts at: 744 744
Size: 3926
StyI C'CwwG_G
Cuts at: 668 859 1060 1414 668
Size: 191 201 354 3180
Fragments arranged by size:
3180 354 201 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 531 1509 1642 2101 2310 531
Size: 978 133 459 209 2147
Fragments arranged by size:
2147 978 459 209 133
TatI w'GTAC_w
Cuts at: 3441 3441
Size: 3926
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3443 3475 3443
Size: 32 3894
Tth111I GACn'n_nGTC
Cuts at: 1723 1723
Size: 3926
XbaI T'CTAG_A
Cuts at: 957 957
Size: 3926
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 857 1421 857
Size: 564 3362
XmaI C'CCGG_G
Cuts at: 946 946
Size: 3926
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BglI BmgBI BmtI
BpmI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PflMI
PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI