Restriction map for
pF1KE0574
(Circular) (Six-base) MAPSORT of: pF1KE0574.seq Check: 594 from: 1 to: 3983
pF1KE0574 3983 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 547 547
Size: 3983
AccI GT'mk_AC
Cuts at: 1021 1021
Size: 3983
Acc65I G'GTAC_C
Cuts at: 999 999
Size: 3983
AclI AA'CG_TT
Cuts at: 3823 3823
Size: 3983
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 707 937 943 1808 2240 2987 3255 214
Size: 493 230 6 865 432 747 268 942
Fragments arranged by size:
942 865 747 493 432 268 230 6
AflIII A'CryG_T
Cuts at: 1335 2439 3416 1335
Size: 1104 977 1902
Fragments arranged by size:
1902 1104 977
AgeI A'CCGG_T
Cuts at: 2371 2371
Size: 3983
AleI CACnn'nnGTG
Cuts at: 819 819
Size: 3983
AlwNI CAG_nnn'CTG
Cuts at: 254 1288 2855 254
Size: 1034 1567 1382
Fragments arranged by size:
1567 1382 1034
ApaLI G'TGCA_C
Cuts at: 2753 2753
Size: 3983
ApoI r'AATT_y
Cuts at: 511 613 746 987 3336 3592 511
Size: 102 133 241 2349 256 902
Fragments arranged by size:
2349 902 256 241 133 102
AseI AT'TA_AT
Cuts at: 20 20
Size: 3983
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3983
AvaI C'yCGr_G
Cuts at: 1003 1003
Size: 3983
BamHI G'GATC_C
Cuts at: 1008 1008
Size: 3983
BanI G'GyrC_C
Cuts at: 999 1413 1661 1696 999
Size: 414 248 35 3286
Fragments arranged by size:
3286 414 248 35
BanII G_rGCy'C
Cuts at: 997 2027 997
Size: 1030 2953
BbeI G_GCGC'C
Cuts at: 1417 1665 1417
Size: 248 3735
BbsI GAAGACnn'nnnn_
Cuts at: 943 943
Size: 3983
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3949
BciVI GTATCCnnnnn_n'
Cuts at: 1881 2642 3983 1881
Size: 761 1341 1881
Fragments arranged by size:
1881 1341 761
BclI T'GATC_A
Cuts at: 1503 1503
Size: 3983
BglII A'GATC_T
Cuts at: 1498 3535 1498
Size: 2037 1946
BlpI GC'TnA_GC
Cuts at: 1095 1095
Size: 3983
Bme1580I G_kGCm'C
Cuts at: 1608 1701 2757 1608
Size: 93 1056 2834
Fragments arranged by size:
2834 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1195 1276 1611 3755 1195
Size: 81 335 2144 1423
Fragments arranged by size:
2144 1423 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 843 2350 843
Size: 1507 2476
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1159 2038 2530 2828 1159
Size: 879 492 298 2314
Fragments arranged by size:
2314 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3687 3687
Size: 3983
BsaAI yAC'GTr
Cuts at: 1336 1336
Size: 3983
BsaBI GATnn'nnATC
Cuts at: 6 1497 3534 6
Size: 1491 2037 455
Fragments arranged by size:
2037 1491 455
BsaHI Gr'CG_yC
Cuts at: 431 1414 1662 2364 3860 431
Size: 983 248 702 1496 554
Fragments arranged by size:
1496 983 702 554 248
BsaWI w'CCGG_w
Cuts at: 155 554 1172 1693 2371 2645 2792 3203 155
Size: 399 618 521 678 274 147 411 935
Fragments arranged by size:
935 678 618 521 411 399 274 147
BseYI C'CCAG_C
Cuts at: 1408 2166 2743 3239 1408
Size: 758 577 496 2152
Fragments arranged by size:
2152 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1525 1571 2779 104
Size: 1421 46 1208 1308
Fragments arranged by size:
1421 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 997 1775 1965 2757 997
Size: 778 190 792 2223
Fragments arranged by size:
2223 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 997 1608 1701 1775 1965 2027 2757 997
Size: 611 93 74 190 62 730 2223
Fragments arranged by size:
2223 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 554 3203 554
Size: 2649 1334
BspHI T'CATG_A
Cuts at: 3159 3159
Size: 3983
BspMI ACCTGCnnnn'nnnn_
Cuts at: 547 1033 1247 1549 1930 547
Size: 486 214 302 381 2600
Fragments arranged by size:
2600 486 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2275 2329 3813 3978 50
Size: 2225 54 1484 165 55
Fragments arranged by size:
2225 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 945 1895 945
Size: 950 3033
BsrFI r'CCGG_y
Cuts at: 1981 2162 2371 1981
Size: 181 209 3593
Fragments arranged by size:
3593 209 181
BssSI C'ACGA_G
Cuts at: 2254 2612 2254
Size: 358 3625
BstAPI GCAn_nnn'nTGC
Cuts at: 364 1360 364
Size: 996 2987
BstBI TT'CG_AA
Cuts at: 891 2344 891
Size: 1453 2530
BstXI CCAn_nnnn'nTGG
Cuts at: 730 730
Size: 3983
BstYI r'GATC_y
Cuts at: 1 1008 1475 1498 1833 2079 3091 3177 3189
Size: 1007 467 23 335 246 1012 86 12
Cuts at: 3189 3535 1
Size: 346 449
Fragments arranged by size:
1012 1007 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 145 867 145
Size: 722 3261
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 589 1933 2114 2277 589
Size: 1344 181 163 2295
Fragments arranged by size:
2295 1344 181 163
BtsI GCAGTG_nn'
Cuts at: 1226 1226
Size: 3983
DraI TTT'AAA
Cuts at: 982 982
Size: 3983
DrdI GACnn_nn'nnGTC
Cuts at: 1689 2547 1689
Size: 858 3125
EaeI y'GGCC_r
Cuts at: 297 453 720 1568 1742 2133 2160 3505 297
Size: 156 267 848 174 391 27 1345 775
Fragments arranged by size:
1345 848 775 391 267 174 156 27
EagI C'GGCC_G
Cuts at: 1568 1568
Size: 3983
EarI CTCTTCn'nnn_
Cuts at: 387 614 2006 2216 387
Size: 227 1392 210 2154
Fragments arranged by size:
2154 1392 227 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2191 2501 2647 3793 2191
Size: 310 146 1146 2381
Fragments arranged by size:
2381 1146 310 146
EcoICRI GAG'CTC
Cuts at: 995 995
Size: 3983
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 707 937 943 1808 2240 2987 3255 214
Size: 493 230 6 865 432 747 268 942
Fragments arranged by size:
942 865 747 493 432 268 230 6
EcoO109I rG'GnC_Cy
Cuts at: 1122 1122
Size: 3983
EcoRI G'AATT_C
Cuts at: 987 987
Size: 3983
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2002 2002
Size: 3983
FspI TGC'GCA
Cuts at: 1764 1764
Size: 3983
HaeII r_GCGC'y
Cuts at: 1417 1665 2687 3666 1417
Size: 248 1022 979 1734
Fragments arranged by size:
1734 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1825 1855 1857 1887 1825
Size: 30 2 30 3921
Fragments arranged by size:
3921 30 30 2
HincII GTy'rAC
Cuts at: 399 1022 399
Size: 623 3360
KasI G'GCGC_C
Cuts at: 1413 1661 1413
Size: 248 3735
KpnI G_GTAC'C
Cuts at: 1003 1003
Size: 3983
MluI A'CGCG_T
Cuts at: 3416 3416
Size: 3983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2654 2838 2654
Size: 184 3799
MscI TGG'CCA
Cuts at: 299 455 722 1744 299
Size: 156 267 1022 2538
Fragments arranged by size:
2538 1022 267 156
MslI CAynn'nnrTG
Cuts at: 815 819 1972 2099 815
Size: 4 1153 127 2699
Fragments arranged by size:
2699 1153 127 4
MspA1I CmG'CkG
Cuts at: 1094 1408 1768 2781 3026 1094
Size: 314 360 1013 245 2051
Fragments arranged by size:
2051 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2164 2164
Size: 3983
NarI GG'CG_CC
Cuts at: 1414 1662 1414
Size: 248 3735
NcoI C'CATG_G
Cuts at: 867 867
Size: 3983
NgoMIV G'CCGG_C
Cuts at: 2162 2162
Size: 3983
NspI r_CATG'y
Cuts at: 1036 1536 2443 1036
Size: 500 907 2576
Fragments arranged by size:
2576 907 500
PciI A'CATG_T
Cuts at: 2439 2439
Size: 3983
PmeI GTTT'AAAC
Cuts at: 982 982
Size: 3983
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3122 3154 3122
Size: 32 3951
PstI C_TGCA'G
Cuts at: 1030 1715 1030
Size: 685 3298
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3983
PvuII CAG'CTG
Cuts at: 1408 1768 1408
Size: 360 3623
RsrII CG'GwC_CG
Cuts at: 2178 2178
Size: 3983
SacI G_AGCT'C
Cuts at: 997 997
Size: 3983
SalI G'TCGA_C
Cuts at: 1020 1020
Size: 3983
SapI GCTCTTCn'nnn_
Cuts at: 2006 2216 2006
Size: 210 3773
SbfI CC_TGCA'GG
Cuts at: 1030 1030
Size: 3983
ScaI AGT'ACT
Cuts at: 3500 3500
Size: 3983
SfcI C'TryA_G
Cuts at: 33 1026 1711 2704 2895 33
Size: 993 685 993 191 1121
Fragments arranged by size:
1121 993 993 685 191
SfoI GGC'GCC
Cuts at: 1415 1663 1415
Size: 248 3735
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3983
SmaI CCC'GGG
Cuts at: 1005 1005
Size: 3983
SmlI C'TyrA_G
Cuts at: 1138 2053 2545 2807 1138
Size: 915 492 262 2314
Fragments arranged by size:
2314 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3983
SphI G_CATG'C
Cuts at: 1036 1536 1036
Size: 500 3483
StyI C'CwwG_G
Cuts at: 729 867 1117 1471 729
Size: 138 250 354 3241
Fragments arranged by size:
3241 354 250 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1566 1699 2158 2367 1566
Size: 133 459 209 3182
Fragments arranged by size:
3182 459 209 133
TatI w'GTAC_w
Cuts at: 804 3498 804
Size: 2694 1289
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 137 169 3500 3532 137
Size: 32 3331 32 588
Fragments arranged by size:
3331 588 32 32
Tth111I GACn'n_nGTC
Cuts at: 1780 1780
Size: 3983
XbaI T'CTAG_A
Cuts at: 507 1014 507
Size: 507 3476
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1478 1478
Size: 3983
XmaI C'CCGG_G
Cuts at: 1003 1003
Size: 3983
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI BpmI
Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI