Restriction map for
pF1KE0573
(Circular) (Six-base) MAPSORT of: pF1KE0573.seq Check: 809 from: 1 to: 3980
pF1KE0573 3980 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 554 1018 554
Size: 464 3516
Acc65I G'GTAC_C
Cuts at: 996 996
Size: 3980
AclI AA'CG_TT
Cuts at: 3820 3820
Size: 3980
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 591 1805 2237 2984 3252 591
Size: 1214 432 747 268 1319
Fragments arranged by size:
1319 1214 747 432 268
AflIII A'CryG_T
Cuts at: 1332 2436 3413 1332
Size: 1104 977 1899
Fragments arranged by size:
1899 1104 977
AgeI A'CCGG_T
Cuts at: 2368 2368
Size: 3980
AlwNI CAG_nnn'CTG
Cuts at: 181 366 480 614 1285 2852 181
Size: 185 114 134 671 1567 1309
Fragments arranged by size:
1567 1309 671 185 134 114
ApaLI G'TGCA_C
Cuts at: 2750 2750
Size: 3980
ApoI r'AATT_y
Cuts at: 984 3333 3589 984
Size: 2349 256 1375
Fragments arranged by size:
2349 1375 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3980
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3980
AvaI C'yCGr_G
Cuts at: 120 520 1000 120
Size: 400 480 3100
Fragments arranged by size:
3100 480 400
BamHI G'GATC_C
Cuts at: 1005 1005
Size: 3980
BanI G'GyrC_C
Cuts at: 460 843 996 1410 1658 1693 460
Size: 383 153 414 248 35 2747
Fragments arranged by size:
2747 414 383 248 153 35
BanII G_rGCy'C
Cuts at: 487 583 706 936 994 2024 487
Size: 96 123 230 58 1030 2443
Fragments arranged by size:
2443 1030 230 123 96 58
BbeI G_GCGC'C
Cuts at: 1414 1662 1414
Size: 248 3732
BbsI GAAGACnn'nnnn_
Cuts at: 290 290
Size: 3980
BciVI GTATCCnnnnn_n'
Cuts at: 1878 2639 3980 1878
Size: 761 1341 1878
Fragments arranged by size:
1878 1341 761
BclI T'GATC_A
Cuts at: 1500 1500
Size: 3980
BglI GCCn_nnn'nGGC
Cuts at: 869 869
Size: 3980
BglII A'GATC_T
Cuts at: 1495 3532 1495
Size: 2037 1943
BlpI GC'TnA_GC
Cuts at: 481 1092 481
Size: 611 3369
Bme1580I G_kGCm'C
Cuts at: 349 463 846 1605 1698 2754 349
Size: 114 383 759 93 1056 1575
Fragments arranged by size:
1575 1056 759 383 114 93
BmgBI CAC'GTC
Cuts at: 966 966
Size: 3980
BmrI ACTGGGnnnn_n'
Cuts at: 1192 1273 1608 3752 1192
Size: 81 335 2144 1420
Fragments arranged by size:
2144 1420 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 146 2347 146
Size: 2201 1779
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 343 388 410 616 819 850 145
Size: 198 45 22 206 203 31 3275
Fragments arranged by size:
3275 206 203 198 45 31 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1156 2035 2527 2825 1156
Size: 879 492 298 2311
Fragments arranged by size:
2311 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 174 205 484 3684 174
Size: 31 279 3200 470
Fragments arranged by size:
3200 470 279 31
BsaAI yAC'GTr
Cuts at: 558 1333 558
Size: 775 3205
BsaBI GATnn'nnATC
Cuts at: 6 1494 3531 6
Size: 1488 2037 455
Fragments arranged by size:
2037 1488 455
BsaHI Gr'CG_yC
Cuts at: 415 907 922 1411 1659 2361 3857 415
Size: 492 15 489 248 702 1496 538
Fragments arranged by size:
1496 702 538 492 489 248 15
BsaWI w'CCGG_w
Cuts at: 902 1169 1690 2368 2642 2789 3200 902
Size: 267 521 678 274 147 411 1682
Fragments arranged by size:
1682 678 521 411 274 267 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 565 595 817 847 565
Size: 30 222 30 3698
Fragments arranged by size:
3698 222 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 162 268 277 469 538 604 162
Size: 106 9 192 69 66 3538
Fragments arranged by size:
3538 192 106 69 66 9
BseYI C'CCAG_C
Cuts at: 194 1405 2163 2740 3236 194
Size: 1211 758 577 496 938
Fragments arranged by size:
1211 938 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 730 454
Size: 276 3704
BsiEI CG_ry'CG
Cuts at: 104 470 940 1522 1568 2776 104
Size: 366 470 582 46 1208 1308
Fragments arranged by size:
1308 1208 582 470 366 46
BsiHKAI G_wGCw'C
Cuts at: 994 1772 1962 2754 994
Size: 778 190 792 2220
Fragments arranged by size:
2220 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 349 463 487 583 706 846 936 994 1605
Size: 114 24 96 123 140 90 58 611
Cuts at: 1605 1698 1772 1962 2024 2754 349
Size: 93 74 190 62 730 1575
Fragments arranged by size:
1575 730 611 190 140 123 114 96
93 90 74 62 58 24
BspEI T'CCGG_A
Cuts at: 3200 3200
Size: 3980
BspHI T'CATG_A
Cuts at: 3156 3156
Size: 3980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1030 1244 1546 1927 1030
Size: 214 302 381 3083
Fragments arranged by size:
3083 381 302 214
BsrBI CCG'CTC
Cuts at: 50 128 2272 2326 3810 3975 50
Size: 78 2144 54 1484 165 55
Fragments arranged by size:
2144 1484 165 78 55 54
BsrDI GCAATG_nn'
Cuts at: 349 1892 349
Size: 1543 2437
BsrFI r'CCGG_y
Cuts at: 909 1978 2159 2368 909
Size: 1069 181 209 2521
Fragments arranged by size:
2521 1069 209 181
BssSI C'ACGA_G
Cuts at: 2251 2609 2251
Size: 358 3622
BstAPI GCAn_nnn'nTGC
Cuts at: 1357 1357
Size: 3980
BstBI TT'CG_AA
Cuts at: 2341 2341
Size: 3980
BstYI r'GATC_y
Cuts at: 1 547 1005 1472 1495 1830 2076 3088 3174
Size: 546 458 467 23 335 246 1012 86
Cuts at: 3174 3186 3532 1
Size: 12 346 449
Fragments arranged by size:
1012 546 467 458 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 182 182
Size: 3980
BtgI C'CryG_G
Cuts at: 395 418 464 494 719 807 395
Size: 23 46 30 225 88 3568
Fragments arranged by size:
3568 225 88 46 30 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1930 2111 2274 1930
Size: 181 163 3636
Fragments arranged by size:
3636 181 163
BtsI GCAGTG_nn'
Cuts at: 1223 1223
Size: 3980
DraI TTT'AAA
Cuts at: 979 979
Size: 3980
DrdI GACnn_nn'nnGTC
Cuts at: 1686 2544 1686
Size: 858 3122
EaeI y'GGCC_r
Cuts at: 467 937 1565 1739 2130 2157 3502 467
Size: 470 628 174 391 27 1345 945
Fragments arranged by size:
1345 945 628 470 391 174 27
EagI C'GGCC_G
Cuts at: 467 937 1565 467
Size: 470 628 2882
Fragments arranged by size:
2882 628 470
EarI CTCTTCn'nnn_
Cuts at: 139 263 446 745 2003 2213 139
Size: 124 183 299 1258 210 1906
Fragments arranged by size:
1906 1258 299 210 183 124
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 573 2188 2498 2644 3790 573
Size: 1615 310 146 1146 763
Fragments arranged by size:
1615 1146 763 310 146
EcoICRI GAG'CTC
Cuts at: 992 992
Size: 3980
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 343 388 410 591 616 819 850 1805
Size: 198 45 22 181 25 203 31 955
Cuts at: 1805 2237 2984 3252 145
Size: 432 747 268 873
Fragments arranged by size:
955 873 747 432 268 203 198 181
45 31 25 22
EcoO109I rG'GnC_Cy
Cuts at: 200 515 813 875 1119 200
Size: 315 298 62 244 3061
Fragments arranged by size:
3061 315 298 244 62
EcoRI G'AATT_C
Cuts at: 984 984
Size: 3980
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1999 1999
Size: 3980
FspI TGC'GCA
Cuts at: 1761 1761
Size: 3980
HaeII r_GCGC'y
Cuts at: 1414 1662 2684 3663 1414
Size: 248 1022 979 1731
Fragments arranged by size:
1731 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 532 564 623 640 655 672 1822 1852 1854
Size: 32 59 17 15 17 1150 30 2
Cuts at: 1854 1884 532
Size: 30 2628
Fragments arranged by size:
2628 1150 59 32 30 30 17 17
15 2
HincII GTy'rAC
Cuts at: 1019 1019
Size: 3980
KasI G'GCGC_C
Cuts at: 1410 1658 1410
Size: 248 3732
KpnI G_GTAC'C
Cuts at: 1000 1000
Size: 3980
MluI A'CGCG_T
Cuts at: 3413 3413
Size: 3980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2651 2835 2651
Size: 184 3796
MscI TGG'CCA
Cuts at: 1741 1741
Size: 3980
MslI CAynn'nnrTG
Cuts at: 379 760 1969 2096 379
Size: 381 1209 127 2263
Fragments arranged by size:
2263 1209 381 127
MspA1I CmG'CkG
Cuts at: 466 477 614 809 860 942 1091 1405 1765
Size: 11 137 195 51 82 149 314 360
Cuts at: 1765 2778 3023 466
Size: 1013 245 1423
Fragments arranged by size:
1423 1013 360 314 245 195 149 137
82 51 11
NaeI GCC'GGC
Cuts at: 911 2161 911
Size: 1250 2730
NarI GG'CG_CC
Cuts at: 1411 1659 1411
Size: 248 3732
NcoI C'CATG_G
Cuts at: 395 395
Size: 3980
NgoMIV G'CCGG_C
Cuts at: 909 2159 909
Size: 1250 2730
NspI r_CATG'y
Cuts at: 1033 1533 2440 1033
Size: 500 907 2573
Fragments arranged by size:
2573 907 500
PasI CC'CwG_GG
Cuts at: 189 787 947 189
Size: 598 160 3222
Fragments arranged by size:
3222 598 160
PciI A'CATG_T
Cuts at: 2436 2436
Size: 3980
PflMI CCAn_nnn'nTGG
Cuts at: 238 366 238
Size: 128 3852
PfoI T'CCnGG_A
Cuts at: 426 426
Size: 3980
PmeI GTTT'AAAC
Cuts at: 979 979
Size: 3980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3119 3151 3119
Size: 32 3948
PpuMI rG'GwC_Cy
Cuts at: 515 515
Size: 3980
PstI C_TGCA'G
Cuts at: 1027 1712 1027
Size: 685 3295
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3980
PvuII CAG'CTG
Cuts at: 477 1405 1765 477
Size: 928 360 2692
Fragments arranged by size:
2692 928 360
RsrII CG'GwC_CG
Cuts at: 2175 2175
Size: 3980
SacI G_AGCT'C
Cuts at: 994 994
Size: 3980
SacII CC_GC'GG
Cuts at: 467 810 467
Size: 343 3637
SalI G'TCGA_C
Cuts at: 1017 1017
Size: 3980
SanDI GG'GwC_CC
Cuts at: 515 515
Size: 3980
SapI GCTCTTCn'nnn_
Cuts at: 2003 2213 2003
Size: 210 3770
SbfI CC_TGCA'GG
Cuts at: 1027 1027
Size: 3980
ScaI AGT'ACT
Cuts at: 3497 3497
Size: 3980
SfcI C'TryA_G
Cuts at: 33 1023 1708 2701 2892 33
Size: 990 685 993 191 1121
Fragments arranged by size:
1121 993 990 685 191
SfoI GGC'GCC
Cuts at: 1412 1660 1412
Size: 248 3732
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3980
SgrAI Cr'CCGG_yG
Cuts at: 909 909
Size: 3980
SmaI CCC'GGG
Cuts at: 1002 1002
Size: 3980
SmlI C'TyrA_G
Cuts at: 1135 2050 2542 2804 1135
Size: 915 492 262 2311
Fragments arranged by size:
2311 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3980
SphI G_CATG'C
Cuts at: 1033 1533 1033
Size: 500 3480
StuI AGG'CCT
Cuts at: 154 154
Size: 3980
StyI C'CwwG_G
Cuts at: 215 395 668 698 1114 1468 215
Size: 180 273 30 416 354 2727
Fragments arranged by size:
2727 416 354 273 180 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 842 939 1563 1696 2155 2364 842
Size: 97 624 133 459 209 2458
Fragments arranged by size:
2458 624 459 209 133 97
TatI w'GTAC_w
Cuts at: 3495 3495
Size: 3980
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 817 849 3497 3529 817
Size: 32 2648 32 1268
Fragments arranged by size:
2648 1268 32 32
Tth111I GACn'n_nGTC
Cuts at: 1777 1777
Size: 3980
XbaI T'CTAG_A
Cuts at: 1011 1011
Size: 3980
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 222 1475 222
Size: 1253 2727
XmaI C'CCGG_G
Cuts at: 1000 1000
Size: 3980
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmtI
Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspOMI PspXI PsrI SexAI
SfiI SnaBI SrfI SspI SwaI XhoI XmnI ZraI