Restriction map for
pF1KE0572
(Circular) (Six-base) MAPSORT of: pF1KE0572.seq Check: 916 from: 1 to: 3872
pF1KE0572 3872 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 910 910
Size: 3872
Acc65I G'GTAC_C
Cuts at: 888 888
Size: 3872
AclI AA'CG_TT
Cuts at: 3712 3712
Size: 3872
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1697 2129 2876 3144 1697
Size: 432 747 268 2425
Fragments arranged by size:
2425 747 432 268
AflIII A'CryG_T
Cuts at: 1224 2328 3305 1224
Size: 1104 977 1791
Fragments arranged by size:
1791 1104 977
AgeI A'CCGG_T
Cuts at: 2260 2260
Size: 3872
AlwNI CAG_nnn'CTG
Cuts at: 235 663 863 1177 2744 235
Size: 428 200 314 1567 1363
Fragments arranged by size:
1567 1363 428 314 200
ApaI G_GGCC'C
Cuts at: 679 679
Size: 3872
ApaLI G'TGCA_C
Cuts at: 2642 2642
Size: 3872
ApoI r'AATT_y
Cuts at: 876 3225 3481 876
Size: 2349 256 1267
Fragments arranged by size:
2349 1267 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3872
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3872
AvaI C'yCGr_G
Cuts at: 193 892 193
Size: 699 3173
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 402 435 402
Size: 33 3839
BamHI G'GATC_C
Cuts at: 897 897
Size: 3872
BanI G'GyrC_C
Cuts at: 111 888 1302 1550 1585 111
Size: 777 414 248 35 2398
Fragments arranged by size:
2398 777 414 248 35
BanII G_rGCy'C
Cuts at: 169 356 452 679 886 1916 169
Size: 187 96 227 207 1030 2125
Fragments arranged by size:
2125 1030 227 207 187 96
BbeI G_GCGC'C
Cuts at: 1306 1554 1306
Size: 248 3624
BbsI GAAGACnn'nnnn_
Cuts at: 792 792
Size: 3872
BciVI GTATCCnnnnn_n'
Cuts at: 1770 2531 3872 1770
Size: 761 1341 1770
Fragments arranged by size:
1770 1341 761
BclI T'GATC_A
Cuts at: 239 1392 239
Size: 1153 2719
BglI GCCn_nnn'nGGC
Cuts at: 437 437
Size: 3872
BglII A'GATC_T
Cuts at: 1387 3424 1387
Size: 2037 1835
BlpI GC'TnA_GC
Cuts at: 224 984 224
Size: 760 3112
Bme1580I G_kGCm'C
Cuts at: 679 1497 1590 2646 679
Size: 818 93 1056 1905
Fragments arranged by size:
1905 1056 818 93
BmrI ACTGGGnnnn_n'
Cuts at: 509 638 749 1084 1165 1500 3644 509
Size: 129 111 335 81 335 2144 737
Fragments arranged by size:
2144 737 335 335 129 111 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 249 2239 249
Size: 1990 1882
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 370 484 535 787 304
Size: 66 114 51 252 3389
Fragments arranged by size:
3389 252 114 66 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1048 1927 2419 2717 1048
Size: 879 492 298 2203
Fragments arranged by size:
2203 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 125 596 783 3576 125
Size: 471 187 2793 421
Fragments arranged by size:
2793 471 421 187
BsaAI yAC'GTr
Cuts at: 1225 1225
Size: 3872
BsaBI GATnn'nnATC
Cuts at: 6 1386 3423 6
Size: 1380 2037 455
Fragments arranged by size:
2037 1380 455
BsaHI Gr'CG_yC
Cuts at: 158 1303 1551 2253 3749 158
Size: 1145 248 702 1496 281
Fragments arranged by size:
1496 1145 702 281 248
BsaWI w'CCGG_w
Cuts at: 266 774 1061 1582 2260 2534 2681 3092 266
Size: 508 287 521 678 274 147 411 1046
Fragments arranged by size:
1046 678 521 508 411 287 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 146 176 527 557 598 628 146
Size: 30 351 30 41 30 3390
Fragments arranged by size:
3390 351 41 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 167 167
Size: 3872
BseYI C'CCAG_C
Cuts at: 451 688 1297 2055 2632 3128 451
Size: 237 609 758 577 496 1195
Fragments arranged by size:
1195 758 609 577 496 237
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 116 116
Size: 3872
BsiEI CG_ry'CG
Cuts at: 104 1414 1460 2668 104
Size: 1310 46 1208 1308
Fragments arranged by size:
1310 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 398 886 1664 1854 2646 398
Size: 488 778 190 792 1624
Fragments arranged by size:
1624 792 778 488 190
BsmBI CGTCTCn'nnnn_
Cuts at: 149 149
Size: 3872
Bsp1286I G_dGCh'C
Cuts at: 169 356 398 452 679 886 1497 1590 1664
Size: 187 42 54 227 207 611 93 74
Cuts at: 1664 1854 1916 2646 169
Size: 190 62 730 1395
Fragments arranged by size:
1395 730 611 227 207 190 187 93
74 62 54 42
BspEI T'CCGG_A
Cuts at: 3092 3092
Size: 3872
BspHI T'CATG_A
Cuts at: 3048 3048
Size: 3872
BspMI ACCTGCnnnn'nnnn_
Cuts at: 729 922 1136 1438 1819 729
Size: 193 214 302 381 2782
Fragments arranged by size:
2782 381 302 214 193
BsrBI CCG'CTC
Cuts at: 50 2164 2218 3702 3867 50
Size: 2114 54 1484 165 55
Fragments arranged by size:
2114 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1784 1784
Size: 3872
BsrFI r'CCGG_y
Cuts at: 1870 2051 2260 1870
Size: 181 209 3482
Fragments arranged by size:
3482 209 181
BssSI C'ACGA_G
Cuts at: 2143 2501 2143
Size: 358 3514
BstAPI GCAn_nnn'nTGC
Cuts at: 735 863 1249 735
Size: 128 386 3358
Fragments arranged by size:
3358 386 128
BstBI TT'CG_AA
Cuts at: 2233 2233
Size: 3872
BstEII G'GTnAC_C
Cuts at: 525 525
Size: 3872
BstXI CCAn_nnnn'nTGG
Cuts at: 329 522 329
Size: 193 3679
BstYI r'GATC_y
Cuts at: 1 897 1364 1387 1722 1968 2980 3066 3078
Size: 896 467 23 335 246 1012 86 12
Cuts at: 3078 3424 1
Size: 346 449
Fragments arranged by size:
1012 896 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 531 531
Size: 3872
BtgI C'CryG_G
Cuts at: 107 115 107
Size: 8 3864
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1822 2003 2166 1822
Size: 181 163 3528
Fragments arranged by size:
3528 181 163
BtsI GCAGTG_nn'
Cuts at: 1115 1115
Size: 3872
DraI TTT'AAA
Cuts at: 871 871
Size: 3872
DrdI GACnn_nn'nnGTC
Cuts at: 1578 2436 1578
Size: 858 3014
EaeI y'GGCC_r
Cuts at: 1457 1631 2022 2049 3394 1457
Size: 174 391 27 1345 1935
Fragments arranged by size:
1935 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1457 1457
Size: 3872
EarI CTCTTCn'nnn_
Cuts at: 1895 2105 1895
Size: 210 3662
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2080 2390 2536 3682 2080
Size: 310 146 1146 2270
Fragments arranged by size:
2270 1146 310 146
EcoICRI GAG'CTC
Cuts at: 884 884
Size: 3872
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 370 484 535 787 1697 2129 2876 3144
Size: 66 114 51 252 910 432 747 268
Cuts at: 3144 304
Size: 1032
Fragments arranged by size:
1032 910 747 432 268 252 114 66
51
EcoO109I rG'GnC_Cy
Cuts at: 278 660 676 1011 278
Size: 382 16 335 3139
Fragments arranged by size:
3139 382 335 16
EcoRI G'AATT_C
Cuts at: 876 876
Size: 3872
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1891 1891
Size: 3872
FspI TGC'GCA
Cuts at: 1653 1653
Size: 3872
HaeII r_GCGC'y
Cuts at: 1306 1554 2576 3555 1306
Size: 248 1022 979 1623
Fragments arranged by size:
1623 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 362 394 1714 1744 1746 1776 362
Size: 32 1320 30 2 30 2458
Fragments arranged by size:
2458 1320 32 30 30 2
HincII GTy'rAC
Cuts at: 911 911
Size: 3872
KasI G'GCGC_C
Cuts at: 1302 1550 1302
Size: 248 3624
KpnI G_GTAC'C
Cuts at: 892 892
Size: 3872
MluI A'CGCG_T
Cuts at: 3305 3305
Size: 3872
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2543 2727 2543
Size: 184 3688
MscI TGG'CCA
Cuts at: 1633 1633
Size: 3872
MslI CAynn'nnrTG
Cuts at: 1861 1988 1861
Size: 127 3745
MspA1I CmG'CkG
Cuts at: 138 416 636 983 1297 1657 2670 2915 138
Size: 278 220 347 314 360 1013 245 1095
Fragments arranged by size:
1095 1013 360 347 314 278 245 220
NaeI GCC'GGC
Cuts at: 2053 2053
Size: 3872
NarI GG'CG_CC
Cuts at: 1303 1551 1303
Size: 248 3624
NcoI C'CATG_G
Cuts at: 107 115 107
Size: 8 3864
NgoMIV G'CCGG_C
Cuts at: 2051 2051
Size: 3872
NspI r_CATG'y
Cuts at: 925 1425 2332 925
Size: 500 907 2465
Fragments arranged by size:
2465 907 500
PasI CC'CwG_GG
Cuts at: 329 329
Size: 3872
PciI A'CATG_T
Cuts at: 2328 2328
Size: 3872
PflMI CCAn_nnn'nTGG
Cuts at: 218 218
Size: 3872
PfoI T'CCnGG_A
Cuts at: 616 764 616
Size: 148 3724
PmeI GTTT'AAAC
Cuts at: 871 871
Size: 3872
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3011 3043 3011
Size: 32 3840
PpuMI rG'GwC_Cy
Cuts at: 278 278
Size: 3872
PspOMI G'GGCC_C
Cuts at: 675 675
Size: 3872
PstI C_TGCA'G
Cuts at: 143 438 740 919 1604 143
Size: 295 302 179 685 2411
Fragments arranged by size:
2411 685 302 295 179
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3872
PvuII CAG'CTG
Cuts at: 138 636 1297 1657 138
Size: 498 661 360 2353
Fragments arranged by size:
2353 661 498 360
RsrII CG'GwC_CG
Cuts at: 2067 2067
Size: 3872
SacI G_AGCT'C
Cuts at: 886 886
Size: 3872
SalI G'TCGA_C
Cuts at: 909 909
Size: 3872
SanDI GG'GwC_CC
Cuts at: 278 278
Size: 3872
SapI GCTCTTCn'nnn_
Cuts at: 1895 2105 1895
Size: 210 3662
SbfI CC_TGCA'GG
Cuts at: 438 740 919 438
Size: 302 179 3391
Fragments arranged by size:
3391 302 179
ScaI AGT'ACT
Cuts at: 3389 3389
Size: 3872
SfcI C'TryA_G
Cuts at: 33 139 426 434 736 915 1600 2593 2784
Size: 106 287 8 302 179 685 993 191
Cuts at: 2784 33
Size: 1121
Fragments arranged by size:
1121 993 685 302 287 191 179 106
8
SfoI GGC'GCC
Cuts at: 1304 1552 1304
Size: 248 3624
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3872
SmaI CCC'GGG
Cuts at: 894 894
Size: 3872
SmlI C'TyrA_G
Cuts at: 1027 1942 2434 2696 1027
Size: 915 492 262 2203
Fragments arranged by size:
2203 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3872
SphI G_CATG'C
Cuts at: 925 1425 925
Size: 500 3372
StyI C'CwwG_G
Cuts at: 107 115 623 1006 1360 107
Size: 8 508 383 354 2619
Fragments arranged by size:
2619 508 383 354 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1455 1588 2047 2256 1455
Size: 133 459 209 3071
Fragments arranged by size:
3071 459 209 133
TatI w'GTAC_w
Cuts at: 808 3387 808
Size: 2579 1293
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 146 178 527 559 598 630 3389 3421 146
Size: 32 349 32 39 32 2759 32 597
Fragments arranged by size:
2759 597 349 39 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1669 1669
Size: 3872
XbaI T'CTAG_A
Cuts at: 903 903
Size: 3872
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 459 762 832 1367 459
Size: 303 70 535 2964
Fragments arranged by size:
2964 535 303 70
XmaI C'CCGG_G
Cuts at: 892 892
Size: 3872
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsiWI BsmI BsrGI BssHII BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI