Restriction map for
pF1KE0571
(Circular) (Six-base) MAPSORT of: pF1KE0571.seq Check: 2153 from: 1 to: 3677
pF1KE0571 3677 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 465 465
Size: 3677
AccI GT'mk_AC
Cuts at: 715 715
Size: 3677
Acc65I G'GTAC_C
Cuts at: 693 693
Size: 3677
AclI AA'CG_TT
Cuts at: 3517 3517
Size: 3677
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 1502 1934 2681 2949 415
Size: 1087 432 747 268 1143
Fragments arranged by size:
1143 1087 747 432 268
AflIII A'CryG_T
Cuts at: 1029 2133 3110 1029
Size: 1104 977 1596
Fragments arranged by size:
1596 1104 977
AgeI A'CCGG_T
Cuts at: 2065 2065
Size: 3677
AlwNI CAG_nnn'CTG
Cuts at: 982 2549 982
Size: 1567 2110
ApaLI G'TGCA_C
Cuts at: 2447 2447
Size: 3677
ApoI r'AATT_y
Cuts at: 681 3030 3286 681
Size: 2349 256 1072
Fragments arranged by size:
2349 1072 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3677
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3677
AvaI C'yCGr_G
Cuts at: 697 697
Size: 3677
BamHI G'GATC_C
Cuts at: 702 702
Size: 3677
BanI G'GyrC_C
Cuts at: 209 693 1107 1355 1390 209
Size: 484 414 248 35 2496
Fragments arranged by size:
2496 484 414 248 35
BanII G_rGCy'C
Cuts at: 192 691 1721 192
Size: 499 1030 2148
Fragments arranged by size:
2148 1030 499
BbeI G_GCGC'C
Cuts at: 1111 1359 1111
Size: 248 3429
BbsI GAAGACnn'nnnn_
Cuts at: 573 573
Size: 3677
BciVI GTATCCnnnnn_n'
Cuts at: 1575 2336 3677 1575
Size: 761 1341 1575
Fragments arranged by size:
1575 1341 761
BclI T'GATC_A
Cuts at: 1197 1197
Size: 3677
BglI GCCn_nnn'nGGC
Cuts at: 622 622
Size: 3677
BglII A'GATC_T
Cuts at: 1192 3229 1192
Size: 2037 1640
BlpI GC'TnA_GC
Cuts at: 789 789
Size: 3677
Bme1580I G_kGCm'C
Cuts at: 627 1302 1395 2451 627
Size: 675 93 1056 1853
Fragments arranged by size:
1853 1056 675 93
BmgBI CAC'GTC
Cuts at: 363 363
Size: 3677
BmrI ACTGGGnnnn_n'
Cuts at: 889 970 1305 3449 889
Size: 81 335 2144 1117
Fragments arranged by size:
2144 1117 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2044 2044
Size: 3677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 427 203
Size: 224 3453
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 642 853 1732 2224 2522 253
Size: 389 211 879 492 298 1408
Fragments arranged by size:
1408 879 492 389 298 211
BsaI GGTCTCn'nnnn_
Cuts at: 3381 3381
Size: 3677
BsaAI yAC'GTr
Cuts at: 1030 1030
Size: 3677
BsaBI GATnn'nnATC
Cuts at: 6 1191 3228 6
Size: 1185 2037 455
Fragments arranged by size:
2037 1185 455
BsaHI Gr'CG_yC
Cuts at: 1108 1356 2058 3554 1108
Size: 248 702 1496 1231
Fragments arranged by size:
1496 1231 702 248
BsaWI w'CCGG_w
Cuts at: 866 1387 2065 2339 2486 2897 866
Size: 521 678 274 147 411 1646
Fragments arranged by size:
1646 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 200 200
Size: 3677
BseYI C'CCAG_C
Cuts at: 1102 1860 2437 2933 1102
Size: 758 577 496 1846
Fragments arranged by size:
1846 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1219 1265 2473 104
Size: 1115 46 1208 1308
Fragments arranged by size:
1308 1208 1115 46
BsiHKAI G_wGCw'C
Cuts at: 138 691 1469 1659 2451 138
Size: 553 778 190 792 1364
Fragments arranged by size:
1364 792 778 553 190
Bsp1286I G_dGCh'C
Cuts at: 138 192 627 691 1302 1395 1469 1659 1721
Size: 54 435 64 611 93 74 190 62
Cuts at: 1721 2451 138
Size: 730 1364
Fragments arranged by size:
1364 730 611 435 190 93 74 64
62 54
BspEI T'CCGG_A
Cuts at: 2897 2897
Size: 3677
BspHI T'CATG_A
Cuts at: 2853 2853
Size: 3677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 103 465 727 941 1243 1624 103
Size: 362 262 214 302 381 2156
Fragments arranged by size:
2156 381 362 302 262 214
BsrBI CCG'CTC
Cuts at: 50 1969 2023 3507 3672 50
Size: 1919 54 1484 165 55
Fragments arranged by size:
1919 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 503 1589 503
Size: 1086 2591
BsrFI r'CCGG_y
Cuts at: 1675 1856 2065 1675
Size: 181 209 3287
Fragments arranged by size:
3287 209 181
BssSI C'ACGA_G
Cuts at: 496 1948 2306 496
Size: 1452 358 1867
Fragments arranged by size:
1867 1452 358
BstAPI GCAn_nnn'nTGC
Cuts at: 216 1054 216
Size: 838 2839
BstBI TT'CG_AA
Cuts at: 2038 2038
Size: 3677
BstEII G'GTnAC_C
Cuts at: 444 444
Size: 3677
BstXI CCAn_nnnn'nTGG
Cuts at: 576 576
Size: 3677
BstYI r'GATC_y
Cuts at: 1 702 1169 1192 1527 1773 2785 2871 2883
Size: 701 467 23 335 246 1012 86 12
Cuts at: 2883 3229 1
Size: 346 449
Fragments arranged by size:
1012 701 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3677
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1627 1808 1971 1627
Size: 181 163 3333
Fragments arranged by size:
3333 181 163
BtsI GCAGTG_nn'
Cuts at: 544 920 544
Size: 376 3301
DraI TTT'AAA
Cuts at: 676 676
Size: 3677
DraIII CAC_nnn'GTG
Cuts at: 216 216
Size: 3677
DrdI GACnn_nn'nnGTC
Cuts at: 1383 2241 1383
Size: 858 2819
EaeI y'GGCC_r
Cuts at: 1262 1436 1827 1854 3199 1262
Size: 174 391 27 1345 1740
Fragments arranged by size:
1740 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1262 1262
Size: 3677
EarI CTCTTCn'nnn_
Cuts at: 331 1700 1910 331
Size: 1369 210 2098
Fragments arranged by size:
2098 1369 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 190 361 1885 2195 2341 3487 190
Size: 171 1524 310 146 1146 380
Fragments arranged by size:
1524 1146 380 310 171 146
EcoICRI GAG'CTC
Cuts at: 689 689
Size: 3677
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 415 427 1502 1934 2681 2949 203
Size: 212 12 1075 432 747 268 931
Fragments arranged by size:
1075 931 747 432 268 212 12
EcoNI CCTnn'n_nnAGG
Cuts at: 523 523
Size: 3677
EcoO109I rG'GnC_Cy
Cuts at: 653 816 653
Size: 163 3514
EcoRI G'AATT_C
Cuts at: 681 681
Size: 3677
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1696 1696
Size: 3677
FspI TGC'GCA
Cuts at: 1458 1458
Size: 3677
HaeII r_GCGC'y
Cuts at: 1111 1359 2381 3360 1111
Size: 248 1022 979 1428
Fragments arranged by size:
1428 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 235 251 267 283 1519 1549 1551 1581 235
Size: 16 16 16 1236 30 2 30 2331
Fragments arranged by size:
2331 1236 30 30 16 16 16 2
HincII GTy'rAC
Cuts at: 716 716
Size: 3677
KasI G'GCGC_C
Cuts at: 1107 1355 1107
Size: 248 3429
KpnI G_GTAC'C
Cuts at: 697 697
Size: 3677
MluI A'CGCG_T
Cuts at: 3110 3110
Size: 3677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 314 404 2348 2532 314
Size: 90 1944 184 1459
Fragments arranged by size:
1944 1459 184 90
MscI TGG'CCA
Cuts at: 1438 1438
Size: 3677
MslI CAynn'nnrTG
Cuts at: 1666 1793 1666
Size: 127 3550
MspA1I CmG'CkG
Cuts at: 129 788 1102 1462 2475 2720 129
Size: 659 314 360 1013 245 1086
Fragments arranged by size:
1086 1013 659 360 314 245
NaeI GCC'GGC
Cuts at: 1858 1858
Size: 3677
NarI GG'CG_CC
Cuts at: 1108 1356 1108
Size: 248 3429
NcoI C'CATG_G
Cuts at: 107 107
Size: 3677
NgoMIV G'CCGG_C
Cuts at: 1856 1856
Size: 3677
NspI r_CATG'y
Cuts at: 357 730 1230 2137 357
Size: 373 500 907 1897
Fragments arranged by size:
1897 907 500 373
PciI A'CATG_T
Cuts at: 2133 2133
Size: 3677
PmeI GTTT'AAAC
Cuts at: 676 676
Size: 3677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2816 2848 2816
Size: 32 3645
PstI C_TGCA'G
Cuts at: 476 724 1409 476
Size: 248 685 2744
Fragments arranged by size:
2744 685 248
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3677
PvuII CAG'CTG
Cuts at: 129 1102 1462 129
Size: 973 360 2344
Fragments arranged by size:
2344 973 360
RsrII CG'GwC_CG
Cuts at: 1872 1872
Size: 3677
SacI G_AGCT'C
Cuts at: 691 691
Size: 3677
SalI G'TCGA_C
Cuts at: 714 714
Size: 3677
SapI GCTCTTCn'nnn_
Cuts at: 331 1700 1910 331
Size: 1369 210 2098
Fragments arranged by size:
2098 1369 210
SbfI CC_TGCA'GG
Cuts at: 476 724 476
Size: 248 3429
ScaI AGT'ACT
Cuts at: 3194 3194
Size: 3677
SfcI C'TryA_G
Cuts at: 33 472 720 1405 2398 2589 33
Size: 439 248 685 993 191 1121
Fragments arranged by size:
1121 993 685 439 248 191
SfoI GGC'GCC
Cuts at: 1109 1357 1109
Size: 248 3429
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3677
SmaI CCC'GGG
Cuts at: 699 699
Size: 3677
SmlI C'TyrA_G
Cuts at: 232 657 832 1747 2239 2501 232
Size: 425 175 915 492 262 1408
Fragments arranged by size:
1408 915 492 425 262 175
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3677
SphI G_CATG'C
Cuts at: 357 730 1230 357
Size: 373 500 2804
Fragments arranged by size:
2804 500 373
StyI C'CwwG_G
Cuts at: 107 811 1165 107
Size: 704 354 2619
Fragments arranged by size:
2619 704 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1260 1393 1852 2061 1260
Size: 133 459 209 2876
Fragments arranged by size:
2876 459 209 133
TatI w'GTAC_w
Cuts at: 3192 3192
Size: 3677
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3194 3226 3194
Size: 32 3645
Tth111I GACn'n_nGTC
Cuts at: 1474 1474
Size: 3677
XbaI T'CTAG_A
Cuts at: 708 708
Size: 3677
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 339 1172 339
Size: 833 2844
XmaI C'CCGG_G
Cuts at: 697 697
Size: 3677
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I
Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI