Restriction map for
pF1KE0570
(Circular) (Six-base) MAPSORT of: pF1KE0570.seq Check: 3865 from: 1 to: 3851
pF1KE0570 3851 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 889 889
Size: 3851
Acc65I G'GTAC_C
Cuts at: 867 867
Size: 3851
AclI AA'CG_TT
Cuts at: 3691 3691
Size: 3851
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 521 1676 2108 2855 3123 521
Size: 1155 432 747 268 1249
Fragments arranged by size:
1249 1155 747 432 268
AflIII A'CryG_T
Cuts at: 1203 2307 3284 1203
Size: 1104 977 1770
Fragments arranged by size:
1770 1104 977
AgeI A'CCGG_T
Cuts at: 2239 2239
Size: 3851
AleI CACnn'nnGTG
Cuts at: 252 512 252
Size: 260 3591
AlwNI CAG_nnn'CTG
Cuts at: 1156 2723 1156
Size: 1567 2284
ApaLI G'TGCA_C
Cuts at: 2621 2621
Size: 3851
ApoI r'AATT_y
Cuts at: 855 3204 3460 855
Size: 2349 256 1246
Fragments arranged by size:
2349 1246 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3851
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3851
AvaI C'yCGr_G
Cuts at: 176 871 176
Size: 695 3156
BamHI G'GATC_C
Cuts at: 876 876
Size: 3851
BanI G'GyrC_C
Cuts at: 867 1281 1529 1564 867
Size: 414 248 35 3154
Fragments arranged by size:
3154 414 248 35
BanII G_rGCy'C
Cuts at: 865 1895 865
Size: 1030 2821
BbeI G_GCGC'C
Cuts at: 1285 1533 1285
Size: 248 3603
BciVI GTATCCnnnnn_n'
Cuts at: 1749 2510 3851 1749
Size: 761 1341 1749
Fragments arranged by size:
1749 1341 761
BclI T'GATC_A
Cuts at: 1371 1371
Size: 3851
BglII A'GATC_T
Cuts at: 1366 3403 1366
Size: 2037 1814
BlpI GC'TnA_GC
Cuts at: 963 963
Size: 3851
Bme1580I G_kGCm'C
Cuts at: 1476 1569 2625 1476
Size: 93 1056 2702
Fragments arranged by size:
2702 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 376 1063 1144 1479 3623 376
Size: 687 81 335 2144 604
Fragments arranged by size:
2144 687 604 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 760 2218 760
Size: 1458 2393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 617 742 457
Size: 160 125 3566
Fragments arranged by size:
3566 160 125
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 1027 1906 2398 2696 709
Size: 318 879 492 298 1864
Fragments arranged by size:
1864 879 492 318 298
BsaI GGTCTCn'nnnn_
Cuts at: 3555 3555
Size: 3851
BsaAI yAC'GTr
Cuts at: 1204 1204
Size: 3851
BsaBI GATnn'nnATC
Cuts at: 6 1365 3402 6
Size: 1359 2037 455
Fragments arranged by size:
2037 1359 455
BsaHI Gr'CG_yC
Cuts at: 1282 1530 2232 3728 1282
Size: 248 702 1496 1405
Fragments arranged by size:
1496 1405 702 248
BsaWI w'CCGG_w
Cuts at: 1040 1561 2239 2513 2660 3071 1040
Size: 521 678 274 147 411 1820
Fragments arranged by size:
1820 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 799 799
Size: 3851
BseYI C'CCAG_C
Cuts at: 126 1276 2034 2611 3107 126
Size: 1150 758 577 496 870
Fragments arranged by size:
1150 870 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 752 752
Size: 3851
BsiEI CG_ry'CG
Cuts at: 104 1393 1439 2647 104
Size: 1289 46 1208 1308
Fragments arranged by size:
1308 1289 1208 46
BsiHKAI G_wGCw'C
Cuts at: 741 865 1643 1833 2625 741
Size: 124 778 190 792 1967
Fragments arranged by size:
1967 792 778 190 124
BsmBI CGTCTCn'nnnn_
Cuts at: 182 182
Size: 3851
Bsp1286I G_dGCh'C
Cuts at: 741 865 1476 1569 1643 1833 1895 2625 741
Size: 124 611 93 74 190 62 730 1967
Fragments arranged by size:
1967 730 611 190 124 93 74 62
BspEI T'CCGG_A
Cuts at: 3071 3071
Size: 3851
BspHI T'CATG_A
Cuts at: 3027 3027
Size: 3851
BspMI ACCTGCnnnn'nnnn_
Cuts at: 901 1115 1417 1798 901
Size: 214 302 381 2954
Fragments arranged by size:
2954 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2143 2197 3681 3846 50
Size: 2093 54 1484 165 55
Fragments arranged by size:
2093 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1763 1763
Size: 3851
BsrFI r'CCGG_y
Cuts at: 1849 2030 2239 1849
Size: 181 209 3461
Fragments arranged by size:
3461 209 181
BssSI C'ACGA_G
Cuts at: 2122 2480 2122
Size: 358 3493
BstAPI GCAn_nnn'nTGC
Cuts at: 1228 1228
Size: 3851
BstBI TT'CG_AA
Cuts at: 2212 2212
Size: 3851
BstYI r'GATC_y
Cuts at: 1 517 876 1343 1366 1701 1947 2959 3045
Size: 516 359 467 23 335 246 1012 86
Cuts at: 3045 3057 3403 1
Size: 12 346 449
Fragments arranged by size:
1012 516 467 449 359 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 756 756
Size: 3851
BtgI C'CryG_G
Cuts at: 107 247 107
Size: 140 3711
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1801 1982 2145 1801
Size: 181 163 3507
Fragments arranged by size:
3507 181 163
BtsI GCAGTG_nn'
Cuts at: 519 1094 519
Size: 575 3276
ClaI AT'CG_AT
Cuts at: 536 536
Size: 3851
DraI TTT'AAA
Cuts at: 850 850
Size: 3851
DrdI GACnn_nn'nnGTC
Cuts at: 220 1557 2415 220
Size: 1337 858 1656
Fragments arranged by size:
1656 1337 858
EaeI y'GGCC_r
Cuts at: 641 1436 1610 2001 2028 3373 641
Size: 795 174 391 27 1345 1119
Fragments arranged by size:
1345 1119 795 391 174 27
EagI C'GGCC_G
Cuts at: 1436 1436
Size: 3851
EarI CTCTTCn'nnn_
Cuts at: 565 1874 2084 565
Size: 1309 210 2332
Fragments arranged by size:
2332 1309 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2059 2369 2515 3661 2059
Size: 310 146 1146 2249
Fragments arranged by size:
2249 1146 310 146
EcoICRI GAG'CTC
Cuts at: 863 863
Size: 3851
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 521 617 742 1676 2108 2855 3123 457
Size: 64 96 125 934 432 747 268 1185
Fragments arranged by size:
1185 934 747 432 268 125 96 64
EcoO109I rG'GnC_Cy
Cuts at: 990 990
Size: 3851
EcoRI G'AATT_C
Cuts at: 855 855
Size: 3851
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 500 1870 500
Size: 1370 2481
FspI TGC'GCA
Cuts at: 1632 1632
Size: 3851
HaeII r_GCGC'y
Cuts at: 1285 1533 2555 3534 1285
Size: 248 1022 979 1602
Fragments arranged by size:
1602 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 184 216 1693 1723 1725 1755 184
Size: 32 1477 30 2 30 2280
Fragments arranged by size:
2280 1477 32 30 30 2
HincII GTy'rAC
Cuts at: 890 890
Size: 3851
KasI G'GCGC_C
Cuts at: 1281 1529 1281
Size: 248 3603
KpnI G_GTAC'C
Cuts at: 871 871
Size: 3851
MluI A'CGCG_T
Cuts at: 3284 3284
Size: 3851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2522 2706 2522
Size: 184 3667
MscI TGG'CCA
Cuts at: 643 1612 643
Size: 969 2882
MslI CAynn'nnrTG
Cuts at: 252 512 664 1840 1967 252
Size: 260 152 1176 127 2136
Fragments arranged by size:
2136 1176 260 152 127
MspA1I CmG'CkG
Cuts at: 435 962 1276 1636 2649 2894 435
Size: 527 314 360 1013 245 1392
Fragments arranged by size:
1392 1013 527 360 314 245
NaeI GCC'GGC
Cuts at: 2032 2032
Size: 3851
NarI GG'CG_CC
Cuts at: 1282 1530 1282
Size: 248 3603
NcoI C'CATG_G
Cuts at: 107 107
Size: 3851
NgoMIV G'CCGG_C
Cuts at: 2030 2030
Size: 3851
NspI r_CATG'y
Cuts at: 904 1404 2311 904
Size: 500 907 2444
Fragments arranged by size:
2444 907 500
PasI CC'CwG_GG
Cuts at: 336 336
Size: 3851
PciI A'CATG_T
Cuts at: 2307 2307
Size: 3851
PfoI T'CCnGG_A
Cuts at: 594 594
Size: 3851
PmeI GTTT'AAAC
Cuts at: 850 850
Size: 3851
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2990 3022 2990
Size: 32 3819
PstI C_TGCA'G
Cuts at: 473 898 1583 473
Size: 425 685 2741
Fragments arranged by size:
2741 685 425
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3851
PvuII CAG'CTG
Cuts at: 435 1276 1636 435
Size: 841 360 2650
Fragments arranged by size:
2650 841 360
RsrII CG'GwC_CG
Cuts at: 2046 2046
Size: 3851
SacI G_AGCT'C
Cuts at: 865 865
Size: 3851
SalI G'TCGA_C
Cuts at: 888 888
Size: 3851
SapI GCTCTTCn'nnn_
Cuts at: 1874 2084 1874
Size: 210 3641
SbfI CC_TGCA'GG
Cuts at: 473 898 473
Size: 425 3426
ScaI AGT'ACT
Cuts at: 386 3368 386
Size: 2982 869
SfcI C'TryA_G
Cuts at: 33 469 894 1579 2572 2763 33
Size: 436 425 685 993 191 1121
Fragments arranged by size:
1121 993 685 436 425 191
SfoI GGC'GCC
Cuts at: 1283 1531 1283
Size: 248 3603
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3851
SmaI CCC'GGG
Cuts at: 178 873 178
Size: 695 3156
SmlI C'TyrA_G
Cuts at: 688 1006 1921 2413 2675 688
Size: 318 915 492 262 1864
Fragments arranged by size:
1864 915 492 318 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3851
SphI G_CATG'C
Cuts at: 904 1404 904
Size: 500 3351
StyI C'CwwG_G
Cuts at: 107 452 985 1339 107
Size: 345 533 354 2619
Fragments arranged by size:
2619 533 354 345
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 465 1434 1567 2026 2235 465
Size: 969 133 459 209 2081
Fragments arranged by size:
2081 969 459 209 133
TatI w'GTAC_w
Cuts at: 384 3366 384
Size: 2982 869
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 471 503 3368 3400 471
Size: 32 2865 32 922
Fragments arranged by size:
2865 922 32 32
Tth111I GACn'n_nGTC
Cuts at: 1648 1648
Size: 3851
XbaI T'CTAG_A
Cuts at: 882 882
Size: 3851
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1346 1346
Size: 3851
XmaI C'CCGG_G
Cuts at: 176 871 176
Size: 695 3156
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsrGI BssHII
BstEII BstXI BstZ17I CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI