Restriction map for
pF1KE0569
(Circular) (Six-base) MAPSORT of: pF1KE0569.seq Check: 3702 from: 1 to: 3695
pF1KE0569 3695 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 234 733 234
Size: 499 3196
Acc65I G'GTAC_C
Cuts at: 711 711
Size: 3695
AclI AA'CG_TT
Cuts at: 3535 3535
Size: 3695
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 599 1520 1952 2699 2967 364
Size: 235 921 432 747 268 1092
Fragments arranged by size:
1092 921 747 432 268 235
AflIII A'CryG_T
Cuts at: 648 1047 2151 3128 648
Size: 399 1104 977 1215
Fragments arranged by size:
1215 1104 977 399
AgeI A'CCGG_T
Cuts at: 2083 2083
Size: 3695
AlwNI CAG_nnn'CTG
Cuts at: 264 481 1000 2567 264
Size: 217 519 1567 1392
Fragments arranged by size:
1567 1392 519 217
ApaLI G'TGCA_C
Cuts at: 2465 2465
Size: 3695
ApoI r'AATT_y
Cuts at: 583 699 3048 3304 583
Size: 116 2349 256 974
Fragments arranged by size:
2349 974 256 116
AseI AT'TA_AT
Cuts at: 20 20
Size: 3695
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3695
AvaI C'yCGr_G
Cuts at: 715 715
Size: 3695
BamHI G'GATC_C
Cuts at: 720 720
Size: 3695
BanI G'GyrC_C
Cuts at: 711 1125 1373 1408 711
Size: 414 248 35 2998
Fragments arranged by size:
2998 414 248 35
BanII G_rGCy'C
Cuts at: 709 1739 709
Size: 1030 2665
BbeI G_GCGC'C
Cuts at: 1129 1377 1129
Size: 248 3447
BbsI GAAGACnn'nnnn_
Cuts at: 104 104
Size: 3695
BciVI GTATCCnnnnn_n'
Cuts at: 1593 2354 3695 1593
Size: 761 1341 1593
Fragments arranged by size:
1593 1341 761
BclI T'GATC_A
Cuts at: 1215 1215
Size: 3695
BglII A'GATC_T
Cuts at: 1210 3247 1210
Size: 2037 1658
BlpI GC'TnA_GC
Cuts at: 807 807
Size: 3695
Bme1580I G_kGCm'C
Cuts at: 1320 1413 2469 1320
Size: 93 1056 2546
Fragments arranged by size:
2546 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 907 988 1323 3467 907
Size: 81 335 2144 1135
Fragments arranged by size:
2144 1135 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2062 2062
Size: 3695
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 871 1750 2242 2540 871
Size: 879 492 298 2026
Fragments arranged by size:
2026 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3399 3399
Size: 3695
BsaAI yAC'GTr
Cuts at: 1048 1048
Size: 3695
BsaBI GATnn'nnATC
Cuts at: 6 1209 3246 6
Size: 1203 2037 455
Fragments arranged by size:
2037 1203 455
BsaHI Gr'CG_yC
Cuts at: 1126 1374 2076 3572 1126
Size: 248 702 1496 1249
Fragments arranged by size:
1496 1249 702 248
BsaWI w'CCGG_w
Cuts at: 884 1405 2083 2357 2504 2915 884
Size: 521 678 274 147 411 1664
Fragments arranged by size:
1664 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 107 107
Size: 3695
BseYI C'CCAG_C
Cuts at: 481 592 1120 1878 2455 2951 481
Size: 111 528 758 577 496 1225
Fragments arranged by size:
1225 758 577 528 496 111
BsiEI CG_ry'CG
Cuts at: 104 1237 1283 2491 104
Size: 1133 46 1208 1308
Fragments arranged by size:
1308 1208 1133 46
BsiHKAI G_wGCw'C
Cuts at: 709 1487 1677 2469 709
Size: 778 190 792 1935
Fragments arranged by size:
1935 792 778 190
BsmI GAATG_Cn'
Cuts at: 170 170
Size: 3695
Bsp1286I G_dGCh'C
Cuts at: 709 1320 1413 1487 1677 1739 2469 709
Size: 611 93 74 190 62 730 1935
Fragments arranged by size:
1935 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2915 2915
Size: 3695
BspHI T'CATG_A
Cuts at: 2871 2871
Size: 3695
BspMI ACCTGCnnnn'nnnn_
Cuts at: 745 959 1261 1642 745
Size: 214 302 381 2798
Fragments arranged by size:
2798 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1987 2041 3525 3690 50
Size: 1937 54 1484 165 55
Fragments arranged by size:
1937 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1607 1607
Size: 3695
BsrFI r'CCGG_y
Cuts at: 1693 1874 2083 1693
Size: 181 209 3305
Fragments arranged by size:
3305 209 181
BssSI C'ACGA_G
Cuts at: 1966 2324 1966
Size: 358 3337
BstAPI GCAn_nnn'nTGC
Cuts at: 252 1072 252
Size: 820 2875
BstBI TT'CG_AA
Cuts at: 469 2056 469
Size: 1587 2108
BstYI r'GATC_y
Cuts at: 1 720 1187 1210 1545 1791 2803 2889 2901
Size: 719 467 23 335 246 1012 86 12
Cuts at: 2901 3247 1
Size: 346 449
Fragments arranged by size:
1012 719 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1645 1826 1989 1645
Size: 181 163 3351
Fragments arranged by size:
3351 181 163
BtsI GCAGTG_nn'
Cuts at: 514 938 514
Size: 424 3271
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 342 377 342
Size: 35 3660
DraI TTT'AAA
Cuts at: 694 694
Size: 3695
DrdI GACnn_nn'nnGTC
Cuts at: 1401 2259 1401
Size: 858 2837
EaeI y'GGCC_r
Cuts at: 1280 1454 1845 1872 3217 1280
Size: 174 391 27 1345 1758
Fragments arranged by size:
1758 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1280 1280
Size: 3695
EarI CTCTTCn'nnn_
Cuts at: 1718 1928 1718
Size: 210 3485
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1903 2213 2359 3505 1903
Size: 310 146 1146 2093
Fragments arranged by size:
2093 1146 310 146
EcoICRI GAG'CTC
Cuts at: 707 707
Size: 3695
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 599 1520 1952 2699 2967 364
Size: 235 921 432 747 268 1092
Fragments arranged by size:
1092 921 747 432 268 235
EcoO109I rG'GnC_Cy
Cuts at: 834 834
Size: 3695
EcoRI G'AATT_C
Cuts at: 699 699
Size: 3695
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1714 1714
Size: 3695
FspI TGC'GCA
Cuts at: 1476 1476
Size: 3695
HaeII r_GCGC'y
Cuts at: 1129 1377 2399 3378 1129
Size: 248 1022 979 1446
Fragments arranged by size:
1446 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 589 621 1537 1567 1569 1599 589
Size: 32 916 30 2 30 2685
Fragments arranged by size:
2685 916 32 30 30 2
HincII GTy'rAC
Cuts at: 734 734
Size: 3695
HindIII A'AGCT_T
Cuts at: 464 464
Size: 3695
KasI G'GCGC_C
Cuts at: 1125 1373 1125
Size: 248 3447
KpnI G_GTAC'C
Cuts at: 715 715
Size: 3695
MfeI C'AATT_G
Cuts at: 329 356 329
Size: 27 3668
MluI A'CGCG_T
Cuts at: 3128 3128
Size: 3695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2366 2550 2366
Size: 184 3511
MscI TGG'CCA
Cuts at: 1456 1456
Size: 3695
MslI CAynn'nnrTG
Cuts at: 371 1684 1811 371
Size: 1313 127 2255
Fragments arranged by size:
2255 1313 127
MspA1I CmG'CkG
Cuts at: 806 1120 1480 2493 2738 806
Size: 314 360 1013 245 1763
Fragments arranged by size:
1763 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1876 1876
Size: 3695
NarI GG'CG_CC
Cuts at: 1126 1374 1126
Size: 248 3447
NgoMIV G'CCGG_C
Cuts at: 1874 1874
Size: 3695
NspI r_CATG'y
Cuts at: 422 652 748 1248 2155 422
Size: 230 96 500 907 1962
Fragments arranged by size:
1962 907 500 230 96
PciI A'CATG_T
Cuts at: 648 2151 648
Size: 1503 2192
PmeI GTTT'AAAC
Cuts at: 694 694
Size: 3695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2834 2866 2834
Size: 32 3663
PstI C_TGCA'G
Cuts at: 742 1427 742
Size: 685 3010
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3695
PvuII CAG'CTG
Cuts at: 1120 1480 1120
Size: 360 3335
RsrII CG'GwC_CG
Cuts at: 1890 1890
Size: 3695
SacI G_AGCT'C
Cuts at: 709 709
Size: 3695
SalI G'TCGA_C
Cuts at: 732 732
Size: 3695
SapI GCTCTTCn'nnn_
Cuts at: 1718 1928 1718
Size: 210 3485
SbfI CC_TGCA'GG
Cuts at: 742 742
Size: 3695
ScaI AGT'ACT
Cuts at: 3212 3212
Size: 3695
SfcI C'TryA_G
Cuts at: 33 440 738 1423 2416 2607 33
Size: 407 298 685 993 191 1121
Fragments arranged by size:
1121 993 685 407 298 191
SfoI GGC'GCC
Cuts at: 1127 1375 1127
Size: 248 3447
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3695
SmaI CCC'GGG
Cuts at: 717 717
Size: 3695
SmlI C'TyrA_G
Cuts at: 850 1765 2257 2519 850
Size: 915 492 262 2026
Fragments arranged by size:
2026 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3695
SphI G_CATG'C
Cuts at: 748 1248 748
Size: 500 3195
StyI C'CwwG_G
Cuts at: 829 1183 829
Size: 354 3341
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1278 1411 1870 2079 1278
Size: 133 459 209 2894
Fragments arranged by size:
2894 459 209 133
TatI w'GTAC_w
Cuts at: 504 3210 504
Size: 2706 989
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3212 3244 3212
Size: 32 3663
Tth111I GACn'n_nGTC
Cuts at: 1492 1492
Size: 3695
XbaI T'CTAG_A
Cuts at: 726 726
Size: 3695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1190 1190
Size: 3695
XmaI C'CCGG_G
Cuts at: 715 715
Size: 3695
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI
DraIII EcoNI EcoRV FseI FspAI HpaI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI