Restriction map for
pF1KE0566
(Circular) (Six-base) MAPSORT of: pF1KE0566.seq Check: 5103 from: 1 to: 4334
pF1KE0566 4334 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 269 475 1372 269
Size: 206 897 3231
Fragments arranged by size:
3231 897 206
Acc65I G'GTAC_C
Cuts at: 1350 1350
Size: 4334
AclI AA'CG_TT
Cuts at: 4174 4174
Size: 4334
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2159 2591 3338 3606 2159
Size: 432 747 268 2887
Fragments arranged by size:
2887 747 432 268
AfeI AGC'GCT
Cuts at: 430 430
Size: 4334
AflIII A'CryG_T
Cuts at: 824 1686 2790 3767 824
Size: 862 1104 977 1391
Fragments arranged by size:
1391 1104 977 862
AgeI A'CCGG_T
Cuts at: 2722 2722
Size: 4334
AlwNI CAG_nnn'CTG
Cuts at: 1639 3206 1639
Size: 1567 2767
ApaI G_GGCC'C
Cuts at: 342 342
Size: 4334
ApaLI G'TGCA_C
Cuts at: 1204 3104 1204
Size: 1900 2434
ApoI r'AATT_y
Cuts at: 562 872 952 985 1162 1184 1258 1338 3687
Size: 310 80 33 177 22 74 80 2349
Cuts at: 3687 3943 562
Size: 256 953
Fragments arranged by size:
2349 953 310 256 177 80 80 74
33 22
AseI AT'TA_AT
Cuts at: 20 20
Size: 4334
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4334
AvaI C'yCGr_G
Cuts at: 222 314 380 387 1354 222
Size: 92 66 7 967 3202
Fragments arranged by size:
3202 967 92 66 7
BamHI G'GATC_C
Cuts at: 1359 1359
Size: 4334
BanI G'GyrC_C
Cuts at: 163 283 404 417 1350 1764 2012 2047 163
Size: 120 121 13 933 414 248 35 2450
Fragments arranged by size:
2450 933 414 248 121 120 35 13
BanII G_rGCy'C
Cuts at: 342 1348 2378 342
Size: 1006 1030 2298
Fragments arranged by size:
2298 1030 1006
BbeI G_GCGC'C
Cuts at: 167 287 408 1768 2016 167
Size: 120 121 1360 248 2485
Fragments arranged by size:
2485 1360 248 121 120
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4300
BciVI GTATCCnnnnn_n'
Cuts at: 2232 2993 4334 2232
Size: 761 1341 2232
Fragments arranged by size:
2232 1341 761
BclI T'GATC_A
Cuts at: 1854 1854
Size: 4334
BfrBI ATG'CAT
Cuts at: 603 603
Size: 4334
BglII A'GATC_T
Cuts at: 1849 3886 1849
Size: 2037 2297
BlpI GC'TnA_GC
Cuts at: 1446 1446
Size: 4334
Bme1580I G_kGCm'C
Cuts at: 342 422 1208 1959 2052 3108 342
Size: 80 786 751 93 1056 1568
Fragments arranged by size:
1568 1056 786 751 93 80
BmrI ACTGGGnnnn_n'
Cuts at: 251 499 547 1546 1627 1962 4106 251
Size: 248 48 999 81 335 2144 479
Fragments arranged by size:
2144 999 479 335 248 81 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2701 2701
Size: 4334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 161
Size: 4334
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1510 2389 2881 3179 1510
Size: 879 492 298 2665
Fragments arranged by size:
2665 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 390 569 4038 390
Size: 179 3469 686
Fragments arranged by size:
3469 686 179
BsaAI yAC'GTr
Cuts at: 1687 1687
Size: 4334
BsaBI GATnn'nnATC
Cuts at: 6 1848 3885 6
Size: 1842 2037 455
Fragments arranged by size:
2037 1842 455
BsaHI Gr'CG_yC
Cuts at: 164 211 284 405 1765 2013 2715 4211 164
Size: 47 73 121 1360 248 702 1496 287
Fragments arranged by size:
1496 1360 702 287 248 121 73 47
BsaWI w'CCGG_w
Cuts at: 232 1523 2044 2722 2996 3143 3554 232
Size: 1291 521 678 274 147 411 1012
Fragments arranged by size:
1291 1012 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 190 190
Size: 4334
BseYI C'CCAG_C
Cuts at: 335 1759 2517 3094 3590 335
Size: 1424 758 577 496 1079
Fragments arranged by size:
1424 1079 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 267 267
Size: 4334
BsiEI CG_ry'CG
Cuts at: 104 1876 1922 3130 104
Size: 1772 46 1208 1308
Fragments arranged by size:
1772 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1208 1348 2126 2316 3108 1208
Size: 140 778 190 792 2434
Fragments arranged by size:
2434 792 778 190 140
Bsp1286I G_dGCh'C
Cuts at: 342 422 1208 1348 1959 2052 2126 2316 2378
Size: 80 786 140 611 93 74 190 62
Cuts at: 2378 3108 342
Size: 730 1568
Fragments arranged by size:
1568 786 730 611 190 140 93 80
74 62
BspEI T'CCGG_A
Cuts at: 232 3554 232
Size: 3322 1012
BspHI T'CATG_A
Cuts at: 3510 3510
Size: 4334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 128 1384 1598 1900 2281 128
Size: 1256 214 302 381 2181
Fragments arranged by size:
2181 1256 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2626 2680 4164 4329 50
Size: 2576 54 1484 165 55
Fragments arranged by size:
2576 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2246 2246
Size: 4334
BsrFI r'CCGG_y
Cuts at: 207 414 2332 2513 2722 207
Size: 207 1918 181 209 1819
Fragments arranged by size:
1918 1819 209 207 181
BsrGI T'GTAC_A
Cuts at: 685 685
Size: 4334
BssSI C'ACGA_G
Cuts at: 2605 2963 2605
Size: 358 3976
BstAPI GCAn_nnn'nTGC
Cuts at: 1711 1711
Size: 4334
BstBI TT'CG_AA
Cuts at: 2695 2695
Size: 4334
BstYI r'GATC_y
Cuts at: 1 1359 1826 1849 2184 2430 3442 3528 3540
Size: 1358 467 23 335 246 1012 86 12
Cuts at: 3540 3886 1
Size: 346 449
Fragments arranged by size:
1358 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 476 476
Size: 4334
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2284 2465 2628 2284
Size: 181 163 3990
Fragments arranged by size:
3990 181 163
BtsI GCAGTG_nn'
Cuts at: 1202 1577 1202
Size: 375 3959
DraI TTT'AAA
Cuts at: 567 1333 567
Size: 766 3568
DrdI GACnn_nn'nnGTC
Cuts at: 2040 2898 2040
Size: 858 3476
EaeI y'GGCC_r
Cuts at: 412 1919 2093 2484 2511 3856 412
Size: 1507 174 391 27 1345 890
Fragments arranged by size:
1507 1345 890 391 174 27
EagI C'GGCC_G
Cuts at: 1919 1919
Size: 4334
EarI CTCTTCn'nnn_
Cuts at: 2357 2567 2357
Size: 210 4124
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2542 2852 2998 4144 2542
Size: 310 146 1146 2732
Fragments arranged by size:
2732 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1346 1346
Size: 4334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 2159 2591 3338 3606 161
Size: 1998 432 747 268 889
Fragments arranged by size:
1998 889 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 339 965 1473 339
Size: 626 508 3200
Fragments arranged by size:
3200 626 508
EcoRI G'AATT_C
Cuts at: 1338 1338
Size: 4334
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2353 2353
Size: 4334
FspI TGC'GCA
Cuts at: 2115 2115
Size: 4334
HaeII r_GCGC'y
Cuts at: 167 287 349 408 432 743 1768 2016 3038
Size: 120 62 59 24 311 1025 248 1022
Cuts at: 3038 4017 167
Size: 979 484
Fragments arranged by size:
1025 1022 979 484 311 248 120 62
59 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2176 2206 2208 2238 2176
Size: 30 2 30 4272
Fragments arranged by size:
4272 30 30 2
HincII GTy'rAC
Cuts at: 1373 1373
Size: 4334
KasI G'GCGC_C
Cuts at: 163 283 404 1764 2012 163
Size: 120 121 1360 248 2485
Fragments arranged by size:
2485 1360 248 121 120
KpnI G_GTAC'C
Cuts at: 1354 1354
Size: 4334
MluI A'CGCG_T
Cuts at: 3767 3767
Size: 4334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 511 696 726 3005 3189 511
Size: 185 30 2279 184 1656
Fragments arranged by size:
2279 1656 185 184 30
MscI TGG'CCA
Cuts at: 2095 2095
Size: 4334
MslI CAynn'nnrTG
Cuts at: 2323 2450 2323
Size: 127 4207
MspA1I CmG'CkG
Cuts at: 1445 1759 2119 3132 3377 1445
Size: 314 360 1013 245 2402
Fragments arranged by size:
2402 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2515 2515
Size: 4334
NarI GG'CG_CC
Cuts at: 164 284 405 1765 2013 164
Size: 120 121 1360 248 2485
Fragments arranged by size:
2485 1360 248 121 120
NdeI CA'TA_TG
Cuts at: 1281 1281
Size: 4334
NgoMIV G'CCGG_C
Cuts at: 2513 2513
Size: 4334
NsiI A_TGCA'T
Cuts at: 605 605
Size: 4334
NspI r_CATG'y
Cuts at: 198 828 1387 1887 2794 198
Size: 630 559 500 907 1738
Fragments arranged by size:
1738 907 630 559 500
PciI A'CATG_T
Cuts at: 824 2790 824
Size: 1966 2368
PflMI CCAn_nnn'nTGG
Cuts at: 139 139
Size: 4334
PfoI T'CCnGG_A
Cuts at: 174 174
Size: 4334
PmeI GTTT'AAAC
Cuts at: 1333 1333
Size: 4334
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3473 3505 3473
Size: 32 4302
PpuMI rG'GwC_Cy
Cuts at: 965 965
Size: 4334
PshAI GACnn'nnGTC
Cuts at: 440 440
Size: 4334
PsiI TTA'TAA
Cuts at: 1229 1229
Size: 4334
PspOMI G'GGCC_C
Cuts at: 338 338
Size: 4334
PstI C_TGCA'G
Cuts at: 1381 2066 1381
Size: 685 3649
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4334
PvuII CAG'CTG
Cuts at: 1759 2119 1759
Size: 360 3974
RsrII CG'GwC_CG
Cuts at: 2529 2529
Size: 4334
SacI G_AGCT'C
Cuts at: 1348 1348
Size: 4334
SalI G'TCGA_C
Cuts at: 1371 1371
Size: 4334
SapI GCTCTTCn'nnn_
Cuts at: 2357 2567 2357
Size: 210 4124
SbfI CC_TGCA'GG
Cuts at: 1381 1381
Size: 4334
ScaI AGT'ACT
Cuts at: 240 3851 240
Size: 3611 723
SfcI C'TryA_G
Cuts at: 33 1377 2062 3055 3246 33
Size: 1344 685 993 191 1121
Fragments arranged by size:
1344 1121 993 685 191
SfoI GGC'GCC
Cuts at: 165 285 406 1766 2014 165
Size: 120 121 1360 248 2485
Fragments arranged by size:
2485 1360 248 121 120
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4334
SgrAI Cr'CCGG_yG
Cuts at: 207 207
Size: 4334
SmaI CCC'GGG
Cuts at: 382 1356 382
Size: 974 3360
SmlI C'TyrA_G
Cuts at: 222 1489 2404 2896 3158 222
Size: 1267 915 492 262 1398
Fragments arranged by size:
1398 1267 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4334
SphI G_CATG'C
Cuts at: 198 1387 1887 198
Size: 1189 500 2645
Fragments arranged by size:
2645 1189 500
SspI AAT'ATT
Cuts at: 1031 1240 1031
Size: 209 4125
StuI AGG'CCT
Cuts at: 220 220
Size: 4334
StyI C'CwwG_G
Cuts at: 1468 1822 1468
Size: 354 3980
SwaI ATTT'AAAT
Cuts at: 567 567
Size: 4334
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 233 837 1917 2050 2509 2718 233
Size: 604 1080 133 459 209 1849
Fragments arranged by size:
1849 1080 604 459 209 133
TatI w'GTAC_w
Cuts at: 238 625 685 3849 238
Size: 387 60 3164 723
Fragments arranged by size:
3164 723 387 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3851 3883 3851
Size: 32 4302
Tth111I GACn'n_nGTC
Cuts at: 2131 2131
Size: 4334
XbaI T'CTAG_A
Cuts at: 1365 1365
Size: 4334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1829 1829
Size: 4334
XhoI C'TCGA_G
Cuts at: 222 222
Size: 4334
XmaI C'CCGG_G
Cuts at: 380 1354 380
Size: 974 3360
XmnI GAAnn'nnTTC
Cuts at: 536 536
Size: 4334
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BstZ17I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BglI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmI BsmBI BssHII BstEII BstXI Bsu36I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NheI NotI NruI PacI
PasI PmlI PspXI PsrI SacII SanDI SexAI SfiI
SnaBI SrfI ZraI