Restriction map for
pF1KE0564
(Circular) (Six-base) MAPSORT of: pF1KE0564.seq Check: 9739 from: 1 to: 4457
pF1KE0564 4457 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AatII G_ACGT'C
Cuts at: 1262 1262
Size: 4457
AccI GT'mk_AC
Cuts at: 1495 1495
Size: 4457
Acc65I G'GTAC_C
Cuts at: 1473 1473
Size: 4457
AclI AA'CG_TT
Cuts at: 1187 4297 1187
Size: 3110 1347
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 633 796 1006 2282 2714 3461 3729 633
Size: 163 210 1276 432 747 268 1361
Fragments arranged by size:
1361 1276 747 432 268 210 163
AflIII A'CryG_T
Cuts at: 1289 1809 2913 3890 1289
Size: 520 1104 977 1856
Fragments arranged by size:
1856 1104 977 520
AgeI A'CCGG_T
Cuts at: 2845 2845
Size: 4457
AhdI GACnn_n'nnGTC
Cuts at: 537 1060 537
Size: 523 3934
AleI CACnn'nnGTG
Cuts at: 1366 1366
Size: 4457
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 485 485
Size: 4457
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 949 981 949
Size: 32 4425
AlwNI CAG_nnn'CTG
Cuts at: 317 1251 1762 3329 317
Size: 934 511 1567 1445
Fragments arranged by size:
1567 1445 934 511
ApaI G_GGCC'C
Cuts at: 355 1269 355
Size: 914 3543
ApaLI G'TGCA_C
Cuts at: 3227 3227
Size: 4457
ApoI r'AATT_y
Cuts at: 1461 3810 4066 1461
Size: 2349 256 1852
Fragments arranged by size:
2349 1852 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4457
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4457
AvaI C'yCGr_G
Cuts at: 1477 1477
Size: 4457
BamHI G'GATC_C
Cuts at: 488 719 1482 488
Size: 231 763 3463
Fragments arranged by size:
3463 763 231
BanI G'GyrC_C
Cuts at: 1473 1887 2135 2170 1473
Size: 414 248 35 3760
Fragments arranged by size:
3760 414 248 35
BanII G_rGCy'C
Cuts at: 355 566 1269 1471 2501 355
Size: 211 703 202 1030 2311
Fragments arranged by size:
2311 1030 703 211 202
BbeI G_GCGC'C
Cuts at: 1891 2139 1891
Size: 248 4209
BciVI GTATCCnnnnn_n'
Cuts at: 2355 3116 4457 2355
Size: 761 1341 2355
Fragments arranged by size:
2355 1341 761
BclI T'GATC_A
Cuts at: 1178 1977 1178
Size: 799 3658
BglI GCCn_nnn'nGGC
Cuts at: 634 1201 1442 634
Size: 567 241 3649
Fragments arranged by size:
3649 567 241
BglII A'GATC_T
Cuts at: 1972 4009 1972
Size: 2037 2420
BlpI GC'TnA_GC
Cuts at: 1569 1569
Size: 4457
Bme1580I G_kGCm'C
Cuts at: 355 1269 1373 1388 2082 2175 3231 355
Size: 914 104 15 694 93 1056 1581
Fragments arranged by size:
1581 1056 914 694 104 93 15
BmgBI CAC'GTC
Cuts at: 954 954
Size: 4457
BmrI ACTGGGnnnn_n'
Cuts at: 1669 1750 2085 4229 1669
Size: 81 335 2144 1897
Fragments arranged by size:
2144 1897 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2824 2824
Size: 4457
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 499 550 775 922 1093 1243 1294 1327
Size: 21 51 225 147 171 150 51 33
Cuts at: 1327 478
Size: 3608
Fragments arranged by size:
3608 225 171 150 147 51 51 33
21
Bpu10I CC'TnA_GC
Cuts at: 206 206
Size: 4457
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 1633 2512 3004 3302 136
Size: 1497 879 492 298 1291
Fragments arranged by size:
1497 1291 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4161 4161
Size: 4457
BsaAI yAC'GTr
Cuts at: 1292 1810 1292
Size: 518 3939
BsaBI GATnn'nnATC
Cuts at: 6 1971 4008 6
Size: 1965 2037 455
Fragments arranged by size:
2037 1965 455
BsaHI Gr'CG_yC
Cuts at: 1259 1888 2136 2838 4334 1259
Size: 629 248 702 1496 1382
Fragments arranged by size:
1496 1382 702 629 248
BsaWI w'CCGG_w
Cuts at: 1295 1646 2167 2845 3119 3266 3677 1295
Size: 351 521 678 274 147 411 2075
Fragments arranged by size:
2075 678 521 411 351 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 510 540 775 805 898 928 949 979 1327
Size: 30 235 30 93 30 21 30 348
Cuts at: 1327 1357 510
Size: 30 3610
Fragments arranged by size:
3610 348 235 93 30 30 30 30
30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 574 796 826 919 997 574
Size: 222 30 93 78 4034
Fragments arranged by size:
4034 222 93 78 30
BseYI C'CCAG_C
Cuts at: 1091 1154 1196 1882 2640 3217 3713 1091
Size: 63 42 686 758 577 496 1835
Fragments arranged by size:
1835 758 686 577 496 63 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 857 1341 857
Size: 484 3973
BsiEI CG_ry'CG
Cuts at: 104 423 1999 2045 3253 104
Size: 319 1576 46 1208 1308
Fragments arranged by size:
1576 1308 1208 319 46
BsiHKAI G_wGCw'C
Cuts at: 518 566 1471 2249 2439 3231 518
Size: 48 905 778 190 792 1744
Fragments arranged by size:
1744 905 792 778 190 48
BsmI GAATG_Cn'
Cuts at: 474 603 474
Size: 129 4328
BsmBI CGTCTCn'nnnn_
Cuts at: 1047 1047
Size: 4457
Bsp1286I G_dGCh'C
Cuts at: 355 518 566 1269 1373 1388 1471 2082 2175
Size: 163 48 703 104 15 83 611 93
Cuts at: 2175 2249 2439 2501 3231 355
Size: 74 190 62 730 1581
Fragments arranged by size:
1581 730 703 611 190 163 104 93
83 74 62 48 15
BspEI T'CCGG_A
Cuts at: 3677 3677
Size: 4457
BspHI T'CATG_A
Cuts at: 3633 3633
Size: 4457
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1507 1721 2023 2404 1507
Size: 214 302 381 3560
Fragments arranged by size:
3560 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1227 2749 2803 4287 4452 50
Size: 1177 1522 54 1484 165 55
Fragments arranged by size:
1522 1484 1177 165 55 54
BsrDI GCAATG_nn'
Cuts at: 396 2369 396
Size: 1973 2484
BsrFI r'CCGG_y
Cuts at: 200 2455 2636 2845 200
Size: 2255 181 209 1812
Fragments arranged by size:
2255 1812 209 181
BssSI C'ACGA_G
Cuts at: 2728 3086 2728
Size: 358 4099
BstAPI GCAn_nnn'nTGC
Cuts at: 598 1424 1834 598
Size: 826 410 3221
Fragments arranged by size:
3221 826 410
BstBI TT'CG_AA
Cuts at: 2818 2818
Size: 4457
BstXI CCAn_nnnn'nTGG
Cuts at: 900 900
Size: 4457
BstYI r'GATC_y
Cuts at: 1 488 719 1208 1482 1949 1972 2307 2553
Size: 487 231 489 274 467 23 335 246
Cuts at: 2553 3565 3651 3663 4009 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 489 487 467 449 346 335 274
246 231 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1102 1102
Size: 4457
BtgI C'CryG_G
Cuts at: 236 347 929 236
Size: 111 582 3764
Fragments arranged by size:
3764 582 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2407 2588 2751 2407
Size: 181 163 4113
Fragments arranged by size:
4113 181 163
BtsI GCAGTG_nn'
Cuts at: 1177 1700 1177
Size: 523 3934
DraI TTT'AAA
Cuts at: 1456 1456
Size: 4457
DraIII CAC_nnn'GTG
Cuts at: 740 740
Size: 4457
DrdI GACnn_nn'nnGTC
Cuts at: 2163 3021 2163
Size: 858 3599
EaeI y'GGCC_r
Cuts at: 277 428 674 1001 2042 2216 2607 2634 3979
Size: 151 246 327 1041 174 391 27 1345
Cuts at: 3979 277
Size: 755
Fragments arranged by size:
1345 1041 755 391 327 246 174 151
27
EagI C'GGCC_G
Cuts at: 2042 2042
Size: 4457
EarI CTCTTCn'nnn_
Cuts at: 2480 2690 2480
Size: 210 4247
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2665 2975 3121 4267 2665
Size: 310 146 1146 2855
Fragments arranged by size:
2855 1146 310 146
EcoICRI GAG'CTC
Cuts at: 564 1469 564
Size: 905 3552
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 499 550 633 775 796 922 1006 1093
Size: 21 51 83 142 21 126 84 87
Cuts at: 1093 1243 1294 1327 2282 2714 3461 3729 478
Size: 150 51 33 955 432 747 268 1206
Fragments arranged by size:
1206 955 747 432 268 150 142 126
87 84 83 51 51 33 21 21
EcoNI CCTnn'n_nnAGG
Cuts at: 778 778
Size: 4457
EcoO109I rG'GnC_Cy
Cuts at: 352 377 1596 352
Size: 25 1219 3213
Fragments arranged by size:
3213 1219 25
EcoRI G'AATT_C
Cuts at: 1461 1461
Size: 4457
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2476 2476
Size: 4457
FspI TGC'GCA
Cuts at: 715 2238 715
Size: 1523 2934
HaeII r_GCGC'y
Cuts at: 1891 2139 3161 4140 1891
Size: 248 1022 979 2208
Fragments arranged by size:
2208 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 523 555 900 932 1046 1078 2299 2329 2331
Size: 32 345 32 114 32 1221 30 2
Cuts at: 2331 2361 523
Size: 30 2619
Fragments arranged by size:
2619 1221 345 114 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1065 1496 1065
Size: 431 4026
KasI G'GCGC_C
Cuts at: 1887 2135 1887
Size: 248 4209
KpnI G_GTAC'C
Cuts at: 1477 1477
Size: 4457
MluI A'CGCG_T
Cuts at: 3890 3890
Size: 4457
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3128 3312 3128
Size: 184 4273
MscI TGG'CCA
Cuts at: 430 676 1003 2218 430
Size: 246 327 1215 2669
Fragments arranged by size:
2669 1215 327 246
MslI CAynn'nnrTG
Cuts at: 862 1366 2446 2573 862
Size: 504 1080 127 2746
Fragments arranged by size:
2746 1080 504 127
MspA1I CmG'CkG
Cuts at: 188 287 362 456 687 1158 1200 1568 1882
Size: 99 75 94 231 471 42 368 314
Cuts at: 1882 2242 3255 3500 188
Size: 360 1013 245 1145
Fragments arranged by size:
1145 1013 471 368 360 314 245 231
99 94 75 42
NaeI GCC'GGC
Cuts at: 202 2638 202
Size: 2436 2021
NarI GG'CG_CC
Cuts at: 1888 2136 1888
Size: 248 4209
NcoI C'CATG_G
Cuts at: 236 347 929 236
Size: 111 582 3764
Fragments arranged by size:
3764 582 111
NgoMIV G'CCGG_C
Cuts at: 200 2636 200
Size: 2436 2021
NspI r_CATG'y
Cuts at: 245 1422 1510 2010 2917 245
Size: 1177 88 500 907 1785
Fragments arranged by size:
1785 1177 907 500 88
PasI CC'CwG_GG
Cuts at: 1440 1440
Size: 4457
PciI A'CATG_T
Cuts at: 2913 2913
Size: 4457
PflMI CCAn_nnn'nTGG
Cuts at: 237 999 237
Size: 762 3695
PfoI T'CCnGG_A
Cuts at: 722 722
Size: 4457
PmeI GTTT'AAAC
Cuts at: 1456 1456
Size: 4457
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 949 981 3596 3628 949
Size: 32 2615 32 1778
Fragments arranged by size:
2615 1778 32 32
PspOMI G'GGCC_C
Cuts at: 351 1265 351
Size: 914 3543
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1225 1257 1225
Size: 32 4425
PstI C_TGCA'G
Cuts at: 1088 1504 2189 1088
Size: 416 685 3356
Fragments arranged by size:
3356 685 416
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4457
PvuII CAG'CTG
Cuts at: 287 362 456 687 1158 1200 1882 2242 287
Size: 75 94 231 471 42 682 360 2502
Fragments arranged by size:
2502 682 471 360 231 94 75 42
RsrII CG'GwC_CG
Cuts at: 2652 2652
Size: 4457
SacI G_AGCT'C
Cuts at: 566 1471 566
Size: 905 3552
SalI G'TCGA_C
Cuts at: 1494 1494
Size: 4457
SapI GCTCTTCn'nnn_
Cuts at: 2480 2690 2480
Size: 210 4247
SbfI CC_TGCA'GG
Cuts at: 1504 1504
Size: 4457
ScaI AGT'ACT
Cuts at: 3974 3974
Size: 4457
SexAI A'CCwGG_T
Cuts at: 1244 1244
Size: 4457
SfcI C'TryA_G
Cuts at: 33 1084 1146 1500 2185 3178 3369 33
Size: 1051 62 354 685 993 191 1121
Fragments arranged by size:
1121 1051 993 685 354 191 62
SfoI GGC'GCC
Cuts at: 1889 2137 1889
Size: 248 4209
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4457
SmaI CCC'GGG
Cuts at: 1479 1479
Size: 4457
SmlI C'TyrA_G
Cuts at: 151 1612 2527 3019 3281 151
Size: 1461 915 492 262 1327
Fragments arranged by size:
1461 1327 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4457
SphI G_CATG'C
Cuts at: 245 1422 1510 2010 245
Size: 1177 88 500 2692
Fragments arranged by size:
2692 1177 500 88
StuI AGG'CCT
Cuts at: 499 499
Size: 4457
StyI C'CwwG_G
Cuts at: 236 347 587 929 1591 1945 236
Size: 111 240 342 662 354 2748
Fragments arranged by size:
2748 662 354 342 240 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 165 243 2040 2173 2632 2841 165
Size: 78 1797 133 459 209 1781
Fragments arranged by size:
1797 1781 459 209 133 78
TatI w'GTAC_w
Cuts at: 3972 3972
Size: 4457
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 510 542 775 807 898 930 3974 4006 510
Size: 32 233 32 91 32 3044 32 961
Fragments arranged by size:
3044 961 233 91 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2254 2254
Size: 4457
XbaI T'CTAG_A
Cuts at: 1488 1488
Size: 4457
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 328 684 1952 328
Size: 356 1268 2833
Fragments arranged by size:
2833 1268 356
XmaI C'CCGG_G
Cuts at: 1477 1477
Size: 4457
ZraI GAC'GTC
Cuts at: 1260 1260
Size: 4457
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AscI AvrII BaeI BbsI BbvCI
BcgI BfrBI BmtI BsiWI BsrGI BssHII BstEII BstZ17I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PpuMI
PshAI PsiI PspXI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI