Restriction map for
pF1KE0558
(Circular) (Six-base) MAPSORT of: pF1KE0558.seq Check: 4197 from: 1 to: 4316
pF1KE0558 4316 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1294 1294
Size: 4316
AatII G_ACGT'C
Cuts at: 836 1091 836
Size: 255 4061
AccI GT'mk_AC
Cuts at: 131 509 1354 131
Size: 378 845 3093
Fragments arranged by size:
3093 845 378
Acc65I G'GTAC_C
Cuts at: 1332 1332
Size: 4316
AclI AA'CG_TT
Cuts at: 4156 4156
Size: 4316
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2141 2573 3320 3588 2141
Size: 432 747 268 2869
Fragments arranged by size:
2869 747 432 268
AflIII A'CryG_T
Cuts at: 1668 2772 3749 1668
Size: 1104 977 2235
Fragments arranged by size:
2235 1104 977
AgeI A'CCGG_T
Cuts at: 2704 2704
Size: 4316
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 412 444 880 912 412
Size: 32 436 32 3816
Fragments arranged by size:
3816 436 32 32
AlwNI CAG_nnn'CTG
Cuts at: 210 417 681 684 1621 3188 210
Size: 207 264 3 937 1567 1338
Fragments arranged by size:
1567 1338 937 264 207 3
ApaI G_GGCC'C
Cuts at: 344 969 344
Size: 625 3691
ApaLI G'TGCA_C
Cuts at: 1225 1270 3086 1225
Size: 45 1816 2455
Fragments arranged by size:
2455 1816 45
ApoI r'AATT_y
Cuts at: 862 1320 3669 3925 862
Size: 458 2349 256 1253
Fragments arranged by size:
2349 1253 458 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4316
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4316
AvaI C'yCGr_G
Cuts at: 960 1336 960
Size: 376 3940
BamHI G'GATC_C
Cuts at: 1341 1341
Size: 4316
BanI G'GyrC_C
Cuts at: 613 1040 1332 1746 1994 2029 613
Size: 427 292 414 248 35 2900
Fragments arranged by size:
2900 427 414 292 248 35
BanII G_rGCy'C
Cuts at: 344 969 1073 1330 2360 344
Size: 625 104 257 1030 2300
Fragments arranged by size:
2300 1030 625 257 104
BbeI G_GCGC'C
Cuts at: 617 1044 1750 1998 617
Size: 427 706 248 2935
Fragments arranged by size:
2935 706 427 248
BbsI GAAGACnn'nnnn_
Cuts at: 701 828 701
Size: 127 4189
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 904 938 904
Size: 34 4282
BciVI GTATCCnnnnn_n'
Cuts at: 2214 2975 4316 2214
Size: 761 1341 2214
Fragments arranged by size:
2214 1341 761
BclI T'GATC_A
Cuts at: 1836 1836
Size: 4316
BglI GCCn_nnn'nGGC
Cuts at: 707 707
Size: 4316
BglII A'GATC_T
Cuts at: 1831 3868 1831
Size: 2037 2279
BlpI GC'TnA_GC
Cuts at: 375 1428 375
Size: 1053 3263
Bme1580I G_kGCm'C
Cuts at: 344 643 969 1229 1274 1941 2034 3090 344
Size: 299 326 260 45 667 93 1056 1570
Fragments arranged by size:
1570 1056 667 326 299 260 93 45
BmgBI CAC'GTC
Cuts at: 357 804 357
Size: 447 3869
BmrI ACTGGGnnnn_n'
Cuts at: 1528 1609 1944 4088 1528
Size: 81 335 2144 1756
Fragments arranged by size:
2144 1756 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2683 2683
Size: 4316
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 439 1087 1129 1285 256
Size: 183 648 42 156 3287
Fragments arranged by size:
3287 648 183 156 42
Bpu10I CC'TnA_GC
Cuts at: 1244 1244
Size: 4316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 742 760 1492 2371 2863 3161 742
Size: 18 732 879 492 298 1897
Fragments arranged by size:
1897 879 732 492 298 18
BsaI GGTCTCn'nnnn_
Cuts at: 4020 4020
Size: 4316
BsaAI yAC'GTr
Cuts at: 1669 1669
Size: 4316
BsaBI GATnn'nnATC
Cuts at: 6 1830 3867 6
Size: 1824 2037 455
Fragments arranged by size:
2037 1824 455
BsaHI Gr'CG_yC
Cuts at: 614 833 1041 1088 1747 1995 2697 4193 614
Size: 219 208 47 659 248 702 1496 737
Fragments arranged by size:
1496 737 702 659 248 219 208 47
BsaWI w'CCGG_w
Cuts at: 134 1505 2026 2704 2978 3125 3536 134
Size: 1371 521 678 274 147 411 914
Fragments arranged by size:
1371 914 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 412 442 412
Size: 30 4286
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 673 673
Size: 4316
BseYI C'CCAG_C
Cuts at: 636 1116 1741 2499 3076 3572 636
Size: 480 625 758 577 496 1380
Fragments arranged by size:
1380 758 625 577 496 480
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 516 1030 1144 516
Size: 514 114 3688
Fragments arranged by size:
3688 514 114
BsiEI CG_ry'CG
Cuts at: 104 552 1082 1858 1904 3112 104
Size: 448 530 776 46 1208 1308
Fragments arranged by size:
1308 1208 776 530 448 46
BsiHKAI G_wGCw'C
Cuts at: 1073 1229 1274 1310 1330 2108 2298 3090 1073
Size: 156 45 36 20 778 190 792 2299
Fragments arranged by size:
2299 792 778 190 156 45 36 20
Bsp1286I G_dGCh'C
Cuts at: 344 643 969 1073 1229 1274 1310 1330 1941
Size: 299 326 104 156 45 36 20 611
Cuts at: 1941 2034 2108 2298 2360 3090 344
Size: 93 74 190 62 730 1570
Fragments arranged by size:
1570 730 611 326 299 190 156 104
93 74 62 45 36 20
BspEI T'CCGG_A
Cuts at: 3536 3536
Size: 4316
BspHI T'CATG_A
Cuts at: 3492 3492
Size: 4316
BspMI ACCTGCnnnn'nnnn_
Cuts at: 521 863 1294 1366 1580 1882 2263 521
Size: 342 431 72 214 302 381 2574
Fragments arranged by size:
2574 431 381 342 302 214 72
BsrBI CCG'CTC
Cuts at: 50 588 2608 2662 4146 4311 50
Size: 538 2020 54 1484 165 55
Fragments arranged by size:
2020 1484 538 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2228 2228
Size: 4316
BsrFI r'CCGG_y
Cuts at: 875 1024 2314 2495 2704 875
Size: 149 1290 181 209 2487
Fragments arranged by size:
2487 1290 209 181 149
BssSI C'ACGA_G
Cuts at: 163 1138 1219 2587 2945 163
Size: 975 81 1368 358 1534
Fragments arranged by size:
1534 1368 975 358 81
BstAPI GCAn_nnn'nTGC
Cuts at: 1233 1693 1233
Size: 460 3856
BstBI TT'CG_AA
Cuts at: 2677 2677
Size: 4316
BstXI CCAn_nnnn'nTGG
Cuts at: 327 1221 327
Size: 894 3422
BstYI r'GATC_y
Cuts at: 1 764 1341 1808 1831 2166 2412 3424 3510
Size: 763 577 467 23 335 246 1012 86
Cuts at: 3510 3522 3868 1
Size: 12 346 449
Fragments arranged by size:
1012 763 577 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 326 596 1130 326
Size: 270 534 3512
Fragments arranged by size:
3512 534 270
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 518 920 2266 2447 2610 518
Size: 402 1346 181 163 2224
Fragments arranged by size:
2224 1346 402 181 163
BtsI GCAGTG_nn'
Cuts at: 453 727 1559 453
Size: 274 832 3210
Fragments arranged by size:
3210 832 274
DraI TTT'AAA
Cuts at: 1315 1315
Size: 4316
DrdI GACnn_nn'nnGTC
Cuts at: 832 838 2022 2880 832
Size: 6 1184 858 2268
Fragments arranged by size:
2268 1184 858 6
EaeI y'GGCC_r
Cuts at: 167 1022 1901 2075 2466 2493 3838 167
Size: 855 879 174 391 27 1345 645
Fragments arranged by size:
1345 879 855 645 391 174 27
EagI C'GGCC_G
Cuts at: 1901 1901
Size: 4316
EarI CTCTTCn'nnn_
Cuts at: 448 634 2339 2549 448
Size: 186 1705 210 2215
Fragments arranged by size:
2215 1705 210 186
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 212 556 980 2524 2834 2980 4126 212
Size: 344 424 1544 310 146 1146 402
Fragments arranged by size:
1544 1146 424 402 344 310 146
EcoICRI GAG'CTC
Cuts at: 1071 1328 1071
Size: 257 4059
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 439 1087 1129 1285 2141 2573 3320 3588
Size: 183 648 42 156 856 432 747 268
Cuts at: 3588 256
Size: 984
Fragments arranged by size:
984 856 747 648 432 268 183 156
42
EcoO109I rG'GnC_Cy
Cuts at: 340 341 414 965 966 1455 340
Size: 1 73 551 1 489 3201
Fragments arranged by size:
3201 551 489 73 1 1
EcoRI G'AATT_C
Cuts at: 1320 1320
Size: 4316
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2335 2335
Size: 4316
FspI TGC'GCA
Cuts at: 622 2097 622
Size: 1475 2841
FspAI rTGC'GCAy
Cuts at: 622 622
Size: 4316
HaeII r_GCGC'y
Cuts at: 617 1044 1750 1998 3020 3999 617
Size: 427 706 248 1022 979 934
Fragments arranged by size:
1022 979 934 706 427 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 577 609 2158 2188 2190 2220 577
Size: 32 1549 30 2 30 2673
Fragments arranged by size:
2673 1549 32 30 30 2
HincII GTy'rAC
Cuts at: 843 1355 843
Size: 512 3804
KasI G'GCGC_C
Cuts at: 613 1040 1746 1994 613
Size: 427 706 248 2935
Fragments arranged by size:
2935 706 427 248
KpnI G_GTAC'C
Cuts at: 1336 1336
Size: 4316
MluI A'CGCG_T
Cuts at: 3749 3749
Size: 4316
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 207 2987 3171 207
Size: 2780 184 1352
Fragments arranged by size:
2780 1352 184
MscI TGG'CCA
Cuts at: 2077 2077
Size: 4316
MslI CAynn'nnrTG
Cuts at: 325 1219 2305 2432 325
Size: 894 1086 127 2209
Fragments arranged by size:
2209 1086 894 127
MspA1I CmG'CkG
Cuts at: 270 598 681 1427 1741 2101 3114 3359 270
Size: 328 83 746 314 360 1013 245 1227
Fragments arranged by size:
1227 1013 746 360 328 314 245 83
NaeI GCC'GGC
Cuts at: 1026 2497 1026
Size: 1471 2845
NarI GG'CG_CC
Cuts at: 614 1041 1747 1995 614
Size: 427 706 248 2935
Fragments arranged by size:
2935 706 427 248
NcoI C'CATG_G
Cuts at: 326 326
Size: 4316
NgoMIV G'CCGG_C
Cuts at: 1024 2495 1024
Size: 1471 2845
NspI r_CATG'y
Cuts at: 1104 1369 1869 2776 1104
Size: 265 500 907 2644
Fragments arranged by size:
2644 907 500 265
PciI A'CATG_T
Cuts at: 2772 2772
Size: 4316
PflMI CCAn_nnn'nTGG
Cuts at: 417 417
Size: 4316
PfoI T'CCnGG_A
Cuts at: 416 1165 416
Size: 749 3567
PmeI GTTT'AAAC
Cuts at: 1315 1315
Size: 4316
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 412 444 3455 3487 412
Size: 32 3011 32 1241
Fragments arranged by size:
3011 1241 32 32
PpuMI rG'GwC_Cy
Cuts at: 414 414
Size: 4316
PspOMI G'GGCC_C
Cuts at: 340 965 340
Size: 625 3691
PstI C_TGCA'G
Cuts at: 215 722 917 1363 2048 215
Size: 507 195 446 685 2483
Fragments arranged by size:
2483 685 507 446 195
PvuI CG_AT'CG
Cuts at: 104 1082 104
Size: 978 3338
PvuII CAG'CTG
Cuts at: 270 681 1741 2101 270
Size: 411 1060 360 2485
Fragments arranged by size:
2485 1060 411 360
RsrII CG'GwC_CG
Cuts at: 2511 2511
Size: 4316
SacI G_AGCT'C
Cuts at: 1073 1330 1073
Size: 257 4059
SacII CC_GC'GG
Cuts at: 599 599
Size: 4316
SalI G'TCGA_C
Cuts at: 1353 1353
Size: 4316
SapI GCTCTTCn'nnn_
Cuts at: 2339 2549 2339
Size: 210 4106
SbfI CC_TGCA'GG
Cuts at: 1363 1363
Size: 4316
ScaI AGT'ACT
Cuts at: 3833 3833
Size: 4316
SfcI C'TryA_G
Cuts at: 33 211 718 913 1359 2044 3037 3228 33
Size: 178 507 195 446 685 993 191 1121
Fragments arranged by size:
1121 993 685 507 446 195 191 178
SfoI GGC'GCC
Cuts at: 615 1042 1748 1996 615
Size: 427 706 248 2935
Fragments arranged by size:
2935 706 427 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4316
SmaI CCC'GGG
Cuts at: 962 1338 962
Size: 376 3940
SmlI C'TyrA_G
Cuts at: 757 775 1471 2386 2878 3140 757
Size: 18 696 915 492 262 1933
Fragments arranged by size:
1933 915 696 492 262 18
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4316
SphI G_CATG'C
Cuts at: 1369 1869 1369
Size: 500 3816
StuI AGG'CCT
Cuts at: 349 349
Size: 4316
StyI C'CwwG_G
Cuts at: 326 1450 1804 326
Size: 1124 354 2838
Fragments arranged by size:
2838 1124 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 243 522 1899 2032 2491 2700 243
Size: 279 1377 133 459 209 1859
Fragments arranged by size:
1859 1377 459 279 209 133
TatI w'GTAC_w
Cuts at: 3831 3831
Size: 4316
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 257 289 3833 3865 257
Size: 32 3544 32 708
Fragments arranged by size:
3544 708 32 32
Tth111I GACn'n_nGTC
Cuts at: 1087 2113 1087
Size: 1026 3290
XbaI T'CTAG_A
Cuts at: 1347 1347
Size: 4316
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 259 1263 1811 259
Size: 1004 548 2764
Fragments arranged by size:
2764 1004 548
XmaI C'CCGG_G
Cuts at: 960 1336 960
Size: 376 3940
ZraI GAC'GTC
Cuts at: 834 1089 834
Size: 255 4061
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AscI AvrII BaeI
BbvCI BfrBI BmtI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PshAI PsiI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI