Restriction map for
pF1KE0557
(Circular) (Six-base) MAPSORT of: pF1KE0557.seq Check: 2879 from: 1 to: 4463
pF1KE0557 4463 bp
With 182 enzymes: SgfI *
August 18, 2008 14:48 ..
AccI GT'mk_AC
Cuts at: 1394 1501 1394
Size: 107 4356
Acc65I G'GTAC_C
Cuts at: 1479 1479
Size: 4463
AclI AA'CG_TT
Cuts at: 4303 4303
Size: 4463
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1030 2288 2720 3467 3735 1030
Size: 1258 432 747 268 1758
Fragments arranged by size:
1758 1258 747 432 268
AflIII A'CryG_T
Cuts at: 1815 2919 3896 1815
Size: 1104 977 2382
Fragments arranged by size:
2382 1104 977
AgeI A'CCGG_T
Cuts at: 2851 2851
Size: 4463
AhdI GACnn_n'nnGTC
Cuts at: 1256 1256
Size: 4463
AleI CACnn'nnGTG
Cuts at: 647 647
Size: 4463
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 910 942 910
Size: 32 4431
AlwNI CAG_nnn'CTG
Cuts at: 1197 1215 1768 3335 1197
Size: 18 553 1567 2325
Fragments arranged by size:
2325 1567 553 18
ApaI G_GGCC'C
Cuts at: 1149 1149
Size: 4463
ApaLI G'TGCA_C
Cuts at: 3233 3233
Size: 4463
ApoI r'AATT_y
Cuts at: 207 457 1467 3816 4072 207
Size: 250 1010 2349 256 598
Fragments arranged by size:
2349 1010 598 256 250
AseI AT'TA_AT
Cuts at: 20 20
Size: 4463
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4463
AvaI C'yCGr_G
Cuts at: 838 1483 838
Size: 645 3818
BamHI G'GATC_C
Cuts at: 1488 1488
Size: 4463
BanI G'GyrC_C
Cuts at: 1479 1893 2141 2176 1479
Size: 414 248 35 3766
Fragments arranged by size:
3766 414 248 35
BanII G_rGCy'C
Cuts at: 329 1149 1477 2507 329
Size: 820 328 1030 2285
Fragments arranged by size:
2285 1030 820 328
BbeI G_GCGC'C
Cuts at: 1897 2145 1897
Size: 248 4215
BbvCI CC'TCA_GC
Cuts at: 987 987
Size: 4463
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 874 908 874
Size: 34 4429
BciVI GTATCCnnnnn_n'
Cuts at: 2361 3122 4463 2361
Size: 761 1341 2361
Fragments arranged by size:
2361 1341 761
BclI T'GATC_A
Cuts at: 486 1983 486
Size: 1497 2966
BglII A'GATC_T
Cuts at: 711 959 1978 4015 711
Size: 248 1019 2037 1159
Fragments arranged by size:
2037 1159 1019 248
BlpI GC'TnA_GC
Cuts at: 1575 1575
Size: 4463
Bme1580I G_kGCm'C
Cuts at: 1149 2088 2181 3237 1149
Size: 939 93 1056 2375
Fragments arranged by size:
2375 1056 939 93
BmrI ACTGGGnnnn_n'
Cuts at: 1675 1756 2091 4235 1675
Size: 81 335 2144 1903
Fragments arranged by size:
2144 1903 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2830 2830
Size: 4463
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 448 736 824 1096 1171 1219 427
Size: 21 288 88 272 75 48 3671
Fragments arranged by size:
3671 288 272 88 75 48 21
Bpu10I CC'TnA_GC
Cuts at: 987 987
Size: 4463
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 1405 1639 2518 3010 3308 601
Size: 804 234 879 492 298 1756
Fragments arranged by size:
1756 879 804 492 298 234
BsaI GGTCTCn'nnnn_
Cuts at: 1087 1174 1243 4167 1087
Size: 87 69 2924 1383
Fragments arranged by size:
2924 1383 87 69
BsaAI yAC'GTr
Cuts at: 1816 1816
Size: 4463
BsaBI GATnn'nnATC
Cuts at: 6 1977 4014 6
Size: 1971 2037 455
Fragments arranged by size:
2037 1971 455
BsaHI Gr'CG_yC
Cuts at: 1894 2142 2844 4340 1894
Size: 248 702 1496 2017
Fragments arranged by size:
2017 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1652 2173 2851 3125 3272 3683 1652
Size: 521 678 274 147 411 2432
Fragments arranged by size:
2432 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 129 159 709 739 797 827 910 940 129
Size: 30 550 30 58 30 83 30 3652
Fragments arranged by size:
3652 550 83 58 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 424 757 1168 1286 1402 424
Size: 333 411 118 116 3485
Fragments arranged by size:
3485 411 333 118 116
BseYI C'CCAG_C
Cuts at: 1353 1888 2646 3223 3719 1353
Size: 535 758 577 496 2097
Fragments arranged by size:
2097 758 577 535 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 723 1147 723
Size: 424 4039
BsiEI CG_ry'CG
Cuts at: 104 2005 2051 3259 104
Size: 1901 46 1208 1308
Fragments arranged by size:
1901 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1084 1477 2255 2445 3237 1084
Size: 393 778 190 792 2310
Fragments arranged by size:
2310 792 778 393 190
Bsp1286I G_dGCh'C
Cuts at: 329 1084 1149 1477 2088 2181 2255 2445 2507
Size: 755 65 328 611 93 74 190 62
Cuts at: 2507 3237 329
Size: 730 1555
Fragments arranged by size:
1555 755 730 611 328 190 93 74
65 62
BspEI T'CCGG_A
Cuts at: 3683 3683
Size: 4463
BspHI T'CATG_A
Cuts at: 489 3639 489
Size: 3150 1313
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1513 1727 2029 2410 1513
Size: 214 302 381 3566
Fragments arranged by size:
3566 381 302 214
BsrBI CCG'CTC
Cuts at: 50 321 2755 2809 4293 4458 50
Size: 271 2434 54 1484 165 55
Fragments arranged by size:
2434 1484 271 165 55 54
BsrDI GCAATG_nn'
Cuts at: 345 564 1461 2375 345
Size: 219 897 914 2433
Fragments arranged by size:
2433 914 897 219
BsrFI r'CCGG_y
Cuts at: 2461 2642 2851 2461
Size: 181 209 4073
Fragments arranged by size:
4073 209 181
BssSI C'ACGA_G
Cuts at: 2734 3092 2734
Size: 358 4105
BstAPI GCAn_nnn'nTGC
Cuts at: 789 1840 789
Size: 1051 3412
BstBI TT'CG_AA
Cuts at: 2824 2824
Size: 4463
BstEII G'GTnAC_C
Cuts at: 348 348
Size: 4463
BstYI r'GATC_y
Cuts at: 1 711 959 1127 1321 1488 1955 1978 2313
Size: 710 248 168 194 167 467 23 335
Cuts at: 2313 2559 3571 3657 3669 4015 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 710 467 449 346 335 248 246
194 168 167 86 23 12
BtgI C'CryG_G
Cuts at: 477 477
Size: 4463
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 525 2413 2594 2757 525
Size: 1888 181 163 2231
Fragments arranged by size:
2231 1888 181 163
BtsI GCAGTG_nn'
Cuts at: 799 1706 799
Size: 907 3556
ClaI AT'CG_AT
Cuts at: 581 581
Size: 4463
DraI TTT'AAA
Cuts at: 1462 1462
Size: 4463
DrdI GACnn_nn'nnGTC
Cuts at: 2169 3027 2169
Size: 858 3605
EaeI y'GGCC_r
Cuts at: 2048 2222 2613 2640 3985 2048
Size: 174 391 27 1345 2526
Fragments arranged by size:
2526 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2048 2048
Size: 4463
EarI CTCTTCn'nnn_
Cuts at: 119 644 672 931 1028 1433 2486 2696 119
Size: 525 28 259 97 405 1053 210 1886
Fragments arranged by size:
1886 1053 525 405 259 210 97 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2671 2981 3127 4273 2671
Size: 310 146 1146 2861
Fragments arranged by size:
2861 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1475 1475
Size: 4463
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 448 736 824 1030 1096 1171 1219 2288
Size: 21 288 88 206 66 75 48 1069
Cuts at: 2288 2720 3467 3735 427
Size: 432 747 268 1155
Fragments arranged by size:
1155 1069 747 432 288 268 206 88
75 66 48 21
EcoNI CCTnn'n_nnAGG
Cuts at: 739 739
Size: 4463
EcoO109I rG'GnC_Cy
Cuts at: 140 1145 1146 1602 140
Size: 1005 1 456 3001
Fragments arranged by size:
3001 1005 456 1
EcoRI G'AATT_C
Cuts at: 1467 1467
Size: 4463
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2482 2482
Size: 4463
FspI TGC'GCA
Cuts at: 798 1115 2244 798
Size: 317 1129 3017
Fragments arranged by size:
3017 1129 317
HaeII r_GCGC'y
Cuts at: 944 1897 2145 3167 4146 944
Size: 953 248 1022 979 1261
Fragments arranged by size:
1261 1022 979 953 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 129 161 1119 1151 1242 1274 1306 1338 2305
Size: 32 958 32 91 32 32 32 967
Cuts at: 2305 2335 2337 2367 129
Size: 30 2 30 2225
Fragments arranged by size:
2225 967 958 91 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1502 1502
Size: 4463
KasI G'GCGC_C
Cuts at: 1893 2141 1893
Size: 248 4215
KpnI G_GTAC'C
Cuts at: 1483 1483
Size: 4463
MluI A'CGCG_T
Cuts at: 3896 3896
Size: 4463
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 954 3134 3318 954
Size: 2180 184 2099
Fragments arranged by size:
2180 2099 184
MscI TGG'CCA
Cuts at: 2224 2224
Size: 4463
MslI CAynn'nnrTG
Cuts at: 647 2452 2579 647
Size: 1805 127 2531
Fragments arranged by size:
2531 1805 127
MspA1I CmG'CkG
Cuts at: 601 802 1119 1161 1574 1888 2248 3261 3506
Size: 201 317 42 413 314 360 1013 245
Cuts at: 3506 601
Size: 1558
Fragments arranged by size:
1558 1013 413 360 317 314 245 201
42
NaeI GCC'GGC
Cuts at: 2644 2644
Size: 4463
NarI GG'CG_CC
Cuts at: 1894 2142 1894
Size: 248 4215
NgoMIV G'CCGG_C
Cuts at: 2642 2642
Size: 4463
NspI r_CATG'y
Cuts at: 403 873 1516 2016 2923 403
Size: 470 643 500 907 1943
Fragments arranged by size:
1943 907 643 500 470
PciI A'CATG_T
Cuts at: 2919 2919
Size: 4463
PflMI CCAn_nnn'nTGG
Cuts at: 1197 1434 1197
Size: 237 4226
PfoI T'CCnGG_A
Cuts at: 133 1214 133
Size: 1081 3382
PmeI GTTT'AAAC
Cuts at: 1462 1462
Size: 4463
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 910 942 3602 3634 910
Size: 32 2660 32 1739
Fragments arranged by size:
2660 1739 32 32
PpuMI rG'GwC_Cy
Cuts at: 140 140
Size: 4463
PspOMI G'GGCC_C
Cuts at: 1145 1145
Size: 4463
PstI C_TGCA'G
Cuts at: 578 794 953 1510 2195 578
Size: 216 159 557 685 2846
Fragments arranged by size:
2846 685 557 216 159
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4463
PvuII CAG'CTG
Cuts at: 601 802 1119 1161 1888 2248 601
Size: 201 317 42 727 360 2816
Fragments arranged by size:
2816 727 360 317 201 42
RsrII CG'GwC_CG
Cuts at: 2658 2658
Size: 4463
SacI G_AGCT'C
Cuts at: 1477 1477
Size: 4463
SalI G'TCGA_C
Cuts at: 1500 1500
Size: 4463
SapI GCTCTTCn'nnn_
Cuts at: 644 931 2486 2696 644
Size: 287 1555 210 2411
Fragments arranged by size:
2411 1555 287 210
SbfI CC_TGCA'GG
Cuts at: 1510 1510
Size: 4463
ScaI AGT'ACT
Cuts at: 3980 3980
Size: 4463
SexAI A'CCwGG_T
Cuts at: 1166 1166
Size: 4463
SfcI C'TryA_G
Cuts at: 33 495 574 790 949 1506 2191 3184 3375
Size: 462 79 216 159 557 685 993 191
Cuts at: 3375 33
Size: 1121
Fragments arranged by size:
1121 993 685 557 462 216 191 159
79
SfoI GGC'GCC
Cuts at: 1895 2143 1895
Size: 248 4215
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4463
SmaI CCC'GGG
Cuts at: 840 1485 840
Size: 645 3818
SmlI C'TyrA_G
Cuts at: 616 1384 1618 2533 3025 3287 616
Size: 768 234 915 492 262 1792
Fragments arranged by size:
1792 915 768 492 262 234
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4463
SphI G_CATG'C
Cuts at: 403 1516 2016 403
Size: 1113 500 2850
Fragments arranged by size:
2850 1113 500
StuI AGG'CCT
Cuts at: 922 922
Size: 4463
StyI C'CwwG_G
Cuts at: 1597 1951 1597
Size: 354 4109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1111 1198 2046 2179 2638 2847 1111
Size: 87 848 133 459 209 2727
Fragments arranged by size:
2727 848 459 209 133 87
TatI w'GTAC_w
Cuts at: 3978 3978
Size: 4463
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 219 251 797 829 3980 4012 219
Size: 32 546 32 3151 32 670
Fragments arranged by size:
3151 670 546 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2260 2260
Size: 4463
XbaI T'CTAG_A
Cuts at: 1494 1494
Size: 4463
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 423 1365 1958 423
Size: 942 593 2928
Fragments arranged by size:
2928 942 593
XmaI C'CCGG_G
Cuts at: 838 1483 838
Size: 645 3818
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AscI AvrII BaeI
BbsI BfrBI BglI BmgBI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstXI BstZ17I Bsu36I CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI