Restriction map for
pF1KE0554
(Circular) (Six-base) MAPSORT of: pF1KE0554.seq Check: 8728 from: 1 to: 3833
pF1KE0554 3833 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 365 365
Size: 3833
AccI GT'mk_AC
Cuts at: 871 871
Size: 3833
Acc65I G'GTAC_C
Cuts at: 849 849
Size: 3833
AclI AA'CG_TT
Cuts at: 3673 3673
Size: 3833
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1658 2090 2837 3105 1658
Size: 432 747 268 2386
Fragments arranged by size:
2386 747 432 268
AflIII A'CryG_T
Cuts at: 582 1185 2289 3266 582
Size: 603 1104 977 1149
Fragments arranged by size:
1149 1104 977 603
AgeI A'CCGG_T
Cuts at: 2221 2221
Size: 3833
AleI CACnn'nnGTG
Cuts at: 521 521
Size: 3833
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 208 616 208
Size: 408 3425
AlwNI CAG_nnn'CTG
Cuts at: 134 414 479 1138 2705 134
Size: 280 65 659 1567 1262
Fragments arranged by size:
1567 1262 659 280 65
ApaI G_GGCC'C
Cuts at: 336 336
Size: 3833
ApaLI G'TGCA_C
Cuts at: 2603 2603
Size: 3833
ApoI r'AATT_y
Cuts at: 837 3186 3442 837
Size: 2349 256 1228
Fragments arranged by size:
2349 1228 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3833
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3833
AvaI C'yCGr_G
Cuts at: 158 449 853 158
Size: 291 404 3138
Fragments arranged by size:
3138 404 291
BamHI G'GATC_C
Cuts at: 858 858
Size: 3833
BanI G'GyrC_C
Cuts at: 849 1263 1511 1546 849
Size: 414 248 35 3136
Fragments arranged by size:
3136 414 248 35
BanII G_rGCy'C
Cuts at: 336 675 847 1877 336
Size: 339 172 1030 2292
Fragments arranged by size:
2292 1030 339 172
BbeI G_GCGC'C
Cuts at: 1267 1515 1267
Size: 248 3585
BbsI GAAGACnn'nnnn_
Cuts at: 476 476
Size: 3833
BciVI GTATCCnnnnn_n'
Cuts at: 825 1731 2492 3833 825
Size: 906 761 1341 825
Fragments arranged by size:
1341 906 825 761
BclI T'GATC_A
Cuts at: 1353 1353
Size: 3833
BglI GCCn_nnn'nGGC
Cuts at: 180 787 180
Size: 607 3226
BglII A'GATC_T
Cuts at: 1348 3385 1348
Size: 2037 1796
BlpI GC'TnA_GC
Cuts at: 945 945
Size: 3833
Bme1580I G_kGCm'C
Cuts at: 336 1458 1551 2607 336
Size: 1122 93 1056 1562
Fragments arranged by size:
1562 1122 1056 93
BmgBI CAC'GTC
Cuts at: 583 583
Size: 3833
BmrI ACTGGGnnnn_n'
Cuts at: 669 811 1045 1126 1461 3605 669
Size: 142 234 81 335 2144 897
Fragments arranged by size:
2144 897 335 234 142 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2200 2200
Size: 3833
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 221 436 457 221
Size: 215 21 3597
Fragments arranged by size:
3597 215 21
Bpu10I CC'TnA_GC
Cuts at: 674 674
Size: 3833
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1009 1888 2380 2678 1009
Size: 879 492 298 2164
Fragments arranged by size:
2164 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3537 3537
Size: 3833
BsaAI yAC'GTr
Cuts at: 1186 1186
Size: 3833
BsaBI GATnn'nnATC
Cuts at: 6 1347 3384 6
Size: 1341 2037 455
Fragments arranged by size:
2037 1341 455
BsaHI Gr'CG_yC
Cuts at: 1264 1512 2214 3710 1264
Size: 248 702 1496 1387
Fragments arranged by size:
1496 1387 702 248
BsaWI w'CCGG_w
Cuts at: 1022 1543 2221 2495 2642 3053 1022
Size: 521 678 274 147 411 1802
Fragments arranged by size:
1802 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 683 713 683
Size: 30 3803
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 541 837 541
Size: 296 3537
BseYI C'CCAG_C
Cuts at: 302 381 404 576 1258 2016 2593 3089 302
Size: 79 23 172 682 758 577 496 1046
Fragments arranged by size:
1046 758 682 577 496 172 79 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 413
Size: 3833
BsiEI CG_ry'CG
Cuts at: 104 446 1375 1421 2629 104
Size: 342 929 46 1208 1308
Fragments arranged by size:
1308 1208 929 342 46
BsiHKAI G_wGCw'C
Cuts at: 675 847 1625 1815 2607 675
Size: 172 778 190 792 1901
Fragments arranged by size:
1901 792 778 190 172
BsmI GAATG_Cn'
Cuts at: 331 331
Size: 3833
Bsp1286I G_dGCh'C
Cuts at: 336 675 847 1458 1551 1625 1815 1877 2607
Size: 339 172 611 93 74 190 62 730
Cuts at: 2607 336
Size: 1562
Fragments arranged by size:
1562 730 611 339 190 172 93 74
62
BspEI T'CCGG_A
Cuts at: 3053 3053
Size: 3833
BspHI T'CATG_A
Cuts at: 321 3009 321
Size: 2688 1145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 205 365 883 1097 1399 1780 205
Size: 160 518 214 302 381 2258
Fragments arranged by size:
2258 518 381 302 214 160
BsrBI CCG'CTC
Cuts at: 50 774 2125 2179 3663 3828 50
Size: 724 1351 54 1484 165 55
Fragments arranged by size:
1484 1351 724 165 55 54
BsrDI GCAATG_nn'
Cuts at: 593 1745 593
Size: 1152 2681
BsrFI r'CCGG_y
Cuts at: 441 1831 2012 2221 441
Size: 1390 181 209 2053
Fragments arranged by size:
2053 1390 209 181
BsrGI T'GTAC_A
Cuts at: 585 585
Size: 3833
BssSI C'ACGA_G
Cuts at: 2104 2462 2104
Size: 358 3475
BstAPI GCAn_nnn'nTGC
Cuts at: 1210 1210
Size: 3833
BstBI TT'CG_AA
Cuts at: 2194 2194
Size: 3833
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3833
BstYI r'GATC_y
Cuts at: 1 308 858 1325 1348 1683 1929 2941 3027
Size: 307 550 467 23 335 246 1012 86
Cuts at: 3027 3039 3385 1
Size: 12 346 449
Fragments arranged by size:
1012 550 467 449 346 335 307 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 637 767 637
Size: 130 3703
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1783 1964 2127 1783
Size: 181 163 3489
Fragments arranged by size:
3489 181 163
BtsI GCAGTG_nn'
Cuts at: 430 792 1076 430
Size: 362 284 3187
Fragments arranged by size:
3187 362 284
DraI TTT'AAA
Cuts at: 832 832
Size: 3833
DrdI GACnn_nn'nnGTC
Cuts at: 1539 2397 1539
Size: 858 2975
EaeI y'GGCC_r
Cuts at: 125 1418 1592 1983 2010 3355 125
Size: 1293 174 391 27 1345 603
Fragments arranged by size:
1345 1293 603 391 174 27
EagI C'GGCC_G
Cuts at: 1418 1418
Size: 3833
EarI CTCTTCn'nnn_
Cuts at: 1856 2066 1856
Size: 210 3623
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2041 2351 2497 3643 2041
Size: 310 146 1146 2231
Fragments arranged by size:
2231 1146 310 146
EcoICRI GAG'CTC
Cuts at: 673 845 673
Size: 172 3661
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 221 436 457 1658 2090 2837 3105 221
Size: 215 21 1201 432 747 268 949
Fragments arranged by size:
1201 949 747 432 268 215 21
EcoO109I rG'GnC_Cy
Cuts at: 263 332 531 779 972 263
Size: 69 199 248 193 3124
Fragments arranged by size:
3124 248 199 193 69
EcoRI G'AATT_C
Cuts at: 837 837
Size: 3833
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1852 1852
Size: 3833
FspI TGC'GCA
Cuts at: 1614 1614
Size: 3833
HaeII r_GCGC'y
Cuts at: 175 1267 1515 2537 3516 175
Size: 1092 248 1022 979 492
Fragments arranged by size:
1092 1022 979 492 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 136 168 300 332 733 765 1675 1705 1707
Size: 32 132 32 401 32 910 30 2
Cuts at: 1707 1737 136
Size: 30 2232
Fragments arranged by size:
2232 910 401 132 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 872 872
Size: 3833
KasI G'GCGC_C
Cuts at: 1263 1511 1263
Size: 248 3585
KpnI G_GTAC'C
Cuts at: 853 853
Size: 3833
MluI A'CGCG_T
Cuts at: 3266 3266
Size: 3833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 435 2504 2688 435
Size: 2069 184 1580
Fragments arranged by size:
2069 1580 184
MscI TGG'CCA
Cuts at: 127 1594 127
Size: 1467 2366
MslI CAynn'nnrTG
Cuts at: 112 320 521 662 1822 1949 112
Size: 208 201 141 1160 127 1996
Fragments arranged by size:
1996 1160 208 201 141 127
MspA1I CmG'CkG
Cuts at: 479 521 944 1258 1618 2631 2876 479
Size: 42 423 314 360 1013 245 1436
Fragments arranged by size:
1436 1013 423 360 314 245 42
NaeI GCC'GGC
Cuts at: 2014 2014
Size: 3833
NarI GG'CG_CC
Cuts at: 1264 1512 1264
Size: 248 3585
NgoMIV G'CCGG_C
Cuts at: 2012 2012
Size: 3833
NspI r_CATG'y
Cuts at: 886 1386 2293 886
Size: 500 907 2426
Fragments arranged by size:
2426 907 500
PciI A'CATG_T
Cuts at: 2289 2289
Size: 3833
PflMI CCAn_nnn'nTGG
Cuts at: 494 494
Size: 3833
PfoI T'CCnGG_A
Cuts at: 817 817
Size: 3833
PmeI GTTT'AAAC
Cuts at: 832 832
Size: 3833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2972 3004 2972
Size: 32 3801
PpuMI rG'GwC_Cy
Cuts at: 263 263
Size: 3833
PspOMI G'GGCC_C
Cuts at: 332 332
Size: 3833
PstI C_TGCA'G
Cuts at: 623 880 1565 623
Size: 257 685 2891
Fragments arranged by size:
2891 685 257
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3833
PvuII CAG'CTG
Cuts at: 1258 1618 1258
Size: 360 3473
RsrII CG'GwC_CG
Cuts at: 2028 2028
Size: 3833
SacI G_AGCT'C
Cuts at: 675 847 675
Size: 172 3661
SalI G'TCGA_C
Cuts at: 870 870
Size: 3833
SanDI GG'GwC_CC
Cuts at: 263 263
Size: 3833
SapI GCTCTTCn'nnn_
Cuts at: 1856 2066 1856
Size: 210 3623
SbfI CC_TGCA'GG
Cuts at: 880 880
Size: 3833
ScaI AGT'ACT
Cuts at: 3350 3350
Size: 3833
SfcI C'TryA_G
Cuts at: 33 619 876 1561 2554 2745 33
Size: 586 257 685 993 191 1121
Fragments arranged by size:
1121 993 685 586 257 191
SfoI GGC'GCC
Cuts at: 1265 1513 1265
Size: 248 3585
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3833
SmaI CCC'GGG
Cuts at: 160 451 855 160
Size: 291 404 3138
Fragments arranged by size:
3138 404 291
SmlI C'TyrA_G
Cuts at: 988 1903 2395 2657 988
Size: 915 492 262 2164
Fragments arranged by size:
2164 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3833
SphI G_CATG'C
Cuts at: 886 1386 886
Size: 500 3333
StuI AGG'CCT
Cuts at: 372 372
Size: 3833
StyI C'CwwG_G
Cuts at: 336 785 967 1321 336
Size: 449 182 354 2848
Fragments arranged by size:
2848 449 354 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 407 418 1416 1549 2008 2217 407
Size: 11 998 133 459 209 2023
Fragments arranged by size:
2023 998 459 209 133 11
TatI w'GTAC_w
Cuts at: 585 701 3348 585
Size: 116 2647 1070
Fragments arranged by size:
2647 1070 116
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 683 715 3350 3382 683
Size: 32 2635 32 1134
Fragments arranged by size:
2635 1134 32 32
Tth111I GACn'n_nGTC
Cuts at: 1630 1630
Size: 3833
XbaI T'CTAG_A
Cuts at: 864 864
Size: 3833
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1328 1328
Size: 3833
XmaI C'CCGG_G
Cuts at: 158 449 853 158
Size: 291 404 3138
Fragments arranged by size:
3138 404 291
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmtI BsiWI BsmBI BssHII BstEII
BstZ17I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI