Restriction map for
pF1KE0548
(Circular) (Six-base) MAPSORT of: pF1KE0548.seq Check: 3154 from: 1 to: 3611
pF1KE0548 3611 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 649 649
Size: 3611
Acc65I G'GTAC_C
Cuts at: 627 627
Size: 3611
AclI AA'CG_TT
Cuts at: 3451 3451
Size: 3611
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 610 1436 1868 2615 2883 610
Size: 826 432 747 268 1338
Fragments arranged by size:
1338 826 747 432 268
AflIII A'CryG_T
Cuts at: 963 2067 3044 963
Size: 1104 977 1530
Fragments arranged by size:
1530 1104 977
AgeI A'CCGG_T
Cuts at: 1999 1999
Size: 3611
AleI CACnn'nnGTG
Cuts at: 372 372
Size: 3611
AlwNI CAG_nnn'CTG
Cuts at: 916 2483 916
Size: 1567 2044
ApaLI G'TGCA_C
Cuts at: 2381 2381
Size: 3611
ApoI r'AATT_y
Cuts at: 615 2964 3220 615
Size: 2349 256 1006
Fragments arranged by size:
2349 1006 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3611
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3611
AvaI C'yCGr_G
Cuts at: 631 631
Size: 3611
BamHI G'GATC_C
Cuts at: 636 636
Size: 3611
BanI G'GyrC_C
Cuts at: 504 627 1041 1289 1324 504
Size: 123 414 248 35 2791
Fragments arranged by size:
2791 414 248 123 35
BanII G_rGCy'C
Cuts at: 201 625 1655 201
Size: 424 1030 2157
Fragments arranged by size:
2157 1030 424
BbeI G_GCGC'C
Cuts at: 1045 1293 1045
Size: 248 3363
BciVI GTATCCnnnnn_n'
Cuts at: 1509 2270 3611 1509
Size: 761 1341 1509
Fragments arranged by size:
1509 1341 761
BclI T'GATC_A
Cuts at: 1131 1131
Size: 3611
BglII A'GATC_T
Cuts at: 1126 3163 1126
Size: 2037 1574
BlpI GC'TnA_GC
Cuts at: 113 723 113
Size: 610 3001
Bme1580I G_kGCm'C
Cuts at: 509 1236 1329 2385 509
Size: 727 93 1056 1735
Fragments arranged by size:
1735 1056 727 93
BmgBI CAC'GTC
Cuts at: 537 537
Size: 3611
BmrI ACTGGGnnnn_n'
Cuts at: 823 904 1239 3383 823
Size: 81 335 2144 1051
Fragments arranged by size:
2144 1051 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1978 1978
Size: 3611
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 138 138
Size: 3611
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 787 1666 2158 2456 787
Size: 879 492 298 1942
Fragments arranged by size:
1942 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3315 3315
Size: 3611
BsaAI yAC'GTr
Cuts at: 964 964
Size: 3611
BsaBI GATnn'nnATC
Cuts at: 6 569 1125 3162 6
Size: 563 556 2037 455
Fragments arranged by size:
2037 563 556 455
BsaHI Gr'CG_yC
Cuts at: 1042 1290 1992 3488 1042
Size: 248 702 1496 1165
Fragments arranged by size:
1496 1165 702 248
BsaWI w'CCGG_w
Cuts at: 800 1321 1999 2273 2420 2831 800
Size: 521 678 274 147 411 1580
Fragments arranged by size:
1580 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 144 144
Size: 3611
BseYI C'CCAG_C
Cuts at: 249 1036 1794 2371 2867 249
Size: 787 758 577 496 993
Fragments arranged by size:
993 787 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1153 1199 2407 104
Size: 1049 46 1208 1308
Fragments arranged by size:
1308 1208 1049 46
BsiHKAI G_wGCw'C
Cuts at: 625 1403 1593 2385 625
Size: 778 190 792 1851
Fragments arranged by size:
1851 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 201 509 625 1236 1329 1403 1593 1655 2385
Size: 308 116 611 93 74 190 62 730
Cuts at: 2385 201
Size: 1427
Fragments arranged by size:
1427 730 611 308 190 116 93 74
62
BspEI T'CCGG_A
Cuts at: 2831 2831
Size: 3611
BspHI T'CATG_A
Cuts at: 2787 2787
Size: 3611
BspMI ACCTGCnnnn'nnnn_
Cuts at: 661 875 1177 1558 661
Size: 214 302 381 2714
Fragments arranged by size:
2714 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1903 1957 3441 3606 50
Size: 1853 54 1484 165 55
Fragments arranged by size:
1853 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1523 1523
Size: 3611
BsrFI r'CCGG_y
Cuts at: 1609 1790 1999 1609
Size: 181 209 3221
Fragments arranged by size:
3221 209 181
BssSI C'ACGA_G
Cuts at: 270 1882 2240 270
Size: 1612 358 1641
Fragments arranged by size:
1641 1612 358
BstAPI GCAn_nnn'nTGC
Cuts at: 988 988
Size: 3611
BstBI TT'CG_AA
Cuts at: 1972 1972
Size: 3611
BstXI CCAn_nnnn'nTGG
Cuts at: 516 516
Size: 3611
BstYI r'GATC_y
Cuts at: 1 275 389 636 1103 1126 1461 1707 2719
Size: 274 114 247 467 23 335 246 1012
Cuts at: 2719 2805 2817 3163 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 274 247 246
114 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3611
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1561 1742 1905 1561
Size: 181 163 3267
Fragments arranged by size:
3267 181 163
BtsI GCAGTG_nn'
Cuts at: 854 854
Size: 3611
DraI TTT'AAA
Cuts at: 610 610
Size: 3611
DrdI GACnn_nn'nnGTC
Cuts at: 328 1317 2175 328
Size: 989 858 1764
Fragments arranged by size:
1764 989 858
EaeI y'GGCC_r
Cuts at: 178 419 446 518 1196 1370 1761 1788 3133
Size: 241 27 72 678 174 391 27 1345
Cuts at: 3133 178
Size: 656
Fragments arranged by size:
1345 678 656 391 241 174 72 27
27
EagI C'GGCC_G
Cuts at: 1196 1196
Size: 3611
EarI CTCTTCn'nnn_
Cuts at: 1634 1844 1634
Size: 210 3401
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1819 2129 2275 3421 1819
Size: 310 146 1146 2009
Fragments arranged by size:
2009 1146 310 146
EcoICRI GAG'CTC
Cuts at: 623 623
Size: 3611
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 138 610 1436 1868 2615 2883 138
Size: 472 826 432 747 268 866
Fragments arranged by size:
866 826 747 472 432 268
EcoO109I rG'GnC_Cy
Cuts at: 750 750
Size: 3611
EcoRI G'AATT_C
Cuts at: 615 615
Size: 3611
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1630 1630
Size: 3611
FspI TGC'GCA
Cuts at: 1392 1392
Size: 3611
HaeII r_GCGC'y
Cuts at: 1045 1293 2315 3294 1045
Size: 248 1022 979 1362
Fragments arranged by size:
1362 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1453 1483 1485 1515 1453
Size: 30 2 30 3549
Fragments arranged by size:
3549 30 30 2
HincII GTy'rAC
Cuts at: 333 650 333
Size: 317 3294
KasI G'GCGC_C
Cuts at: 1041 1289 1041
Size: 248 3363
KpnI G_GTAC'C
Cuts at: 631 631
Size: 3611
MluI A'CGCG_T
Cuts at: 3044 3044
Size: 3611
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 557 2282 2466 557
Size: 1725 184 1702
Fragments arranged by size:
1725 1702 184
MscI TGG'CCA
Cuts at: 180 421 448 520 1372 180
Size: 241 27 72 852 2419
Fragments arranged by size:
2419 852 241 72 27
MslI CAynn'nnrTG
Cuts at: 372 1600 1727 372
Size: 1228 127 2256
Fragments arranged by size:
2256 1228 127
MspA1I CmG'CkG
Cuts at: 502 722 1036 1396 2409 2654 502
Size: 220 314 360 1013 245 1459
Fragments arranged by size:
1459 1013 360 314 245 220
NaeI GCC'GGC
Cuts at: 1792 1792
Size: 3611
NarI GG'CG_CC
Cuts at: 1042 1290 1042
Size: 248 3363
NcoI C'CATG_G
Cuts at: 107 107
Size: 3611
NgoMIV G'CCGG_C
Cuts at: 1790 1790
Size: 3611
NspI r_CATG'y
Cuts at: 664 1164 2071 664
Size: 500 907 2204
Fragments arranged by size:
2204 907 500
PciI A'CATG_T
Cuts at: 2067 2067
Size: 3611
PfoI T'CCnGG_A
Cuts at: 154 299 154
Size: 145 3466
PmeI GTTT'AAAC
Cuts at: 610 610
Size: 3611
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2750 2782 2750
Size: 32 3579
PstI C_TGCA'G
Cuts at: 206 658 1343 206
Size: 452 685 2474
Fragments arranged by size:
2474 685 452
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3611
PvuII CAG'CTG
Cuts at: 1036 1396 1036
Size: 360 3251
RsrII CG'GwC_CG
Cuts at: 1806 1806
Size: 3611
SacI G_AGCT'C
Cuts at: 625 625
Size: 3611
SalI G'TCGA_C
Cuts at: 648 648
Size: 3611
SapI GCTCTTCn'nnn_
Cuts at: 1634 1844 1634
Size: 210 3401
SbfI CC_TGCA'GG
Cuts at: 658 658
Size: 3611
ScaI AGT'ACT
Cuts at: 3128 3128
Size: 3611
SfcI C'TryA_G
Cuts at: 33 202 654 1339 2332 2523 33
Size: 169 452 685 993 191 1121
Fragments arranged by size:
1121 993 685 452 191 169
SfoI GGC'GCC
Cuts at: 1043 1291 1043
Size: 248 3363
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3611
SmaI CCC'GGG
Cuts at: 633 633
Size: 3611
SmlI C'TyrA_G
Cuts at: 766 1681 2173 2435 766
Size: 915 492 262 1942
Fragments arranged by size:
1942 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3611
SphI G_CATG'C
Cuts at: 664 1164 664
Size: 500 3111
StuI AGG'CCT
Cuts at: 583 583
Size: 3611
StyI C'CwwG_G
Cuts at: 107 449 745 1099 107
Size: 342 296 354 2619
Fragments arranged by size:
2619 354 342 296
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 258 338 1194 1327 1786 1995 258
Size: 80 856 133 459 209 1874
Fragments arranged by size:
1874 856 459 209 133 80
TatI w'GTAC_w
Cuts at: 3126 3126
Size: 3611
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3128 3160 3128
Size: 32 3579
Tth111I GACn'n_nGTC
Cuts at: 1408 1408
Size: 3611
XbaI T'CTAG_A
Cuts at: 642 642
Size: 3611
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1106 1106
Size: 3611
XmaI C'CCGG_G
Cuts at: 631 631
Size: 3611
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI