Restriction map for
pF1KE0537
(Circular) (Six-base) MAPSORT of: pF1KE0537.seq Check: 5870 from: 1 to: 3899
pF1KE0537 3899 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AatII G_ACGT'C
Cuts at: 680 680
Size: 3899
AccI GT'mk_AC
Cuts at: 937 937
Size: 3899
Acc65I G'GTAC_C
Cuts at: 915 915
Size: 3899
AclI AA'CG_TT
Cuts at: 3739 3739
Size: 3899
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1724 2156 2903 3171 1724
Size: 432 747 268 2452
Fragments arranged by size:
2452 747 432 268
AfeI AGC'GCT
Cuts at: 703 703
Size: 3899
AflIII A'CryG_T
Cuts at: 1251 2355 3332 1251
Size: 1104 977 1818
Fragments arranged by size:
1818 1104 977
AgeI A'CCGG_T
Cuts at: 2287 2287
Size: 3899
AlwNI CAG_nnn'CTG
Cuts at: 1204 2771 1204
Size: 1567 2332
ApaLI G'TGCA_C
Cuts at: 2669 2669
Size: 3899
ApoI r'AATT_y
Cuts at: 903 3252 3508 903
Size: 2349 256 1294
Fragments arranged by size:
2349 1294 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3899
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3899
AvaI C'yCGr_G
Cuts at: 653 685 919 653
Size: 32 234 3633
Fragments arranged by size:
3633 234 32
BamHI G'GATC_C
Cuts at: 924 924
Size: 3899
BanI G'GyrC_C
Cuts at: 915 1329 1577 1612 915
Size: 414 248 35 3202
Fragments arranged by size:
3202 414 248 35
BanII G_rGCy'C
Cuts at: 913 1943 913
Size: 1030 2869
BbeI G_GCGC'C
Cuts at: 1333 1581 1333
Size: 248 3651
BbsI GAAGACnn'nnnn_
Cuts at: 672 672
Size: 3899
BbvCI CC'TCA_GC
Cuts at: 445 643 445
Size: 198 3701
BciVI GTATCCnnnnn_n'
Cuts at: 1797 2558 3899 1797
Size: 761 1341 1797
Fragments arranged by size:
1797 1341 761
BclI T'GATC_A
Cuts at: 431 665 1419 431
Size: 234 754 2911
Fragments arranged by size:
2911 754 234
BglII A'GATC_T
Cuts at: 791 1414 3451 791
Size: 623 2037 1239
Fragments arranged by size:
2037 1239 623
BlpI GC'TnA_GC
Cuts at: 287 1011 287
Size: 724 3175
Bme1580I G_kGCm'C
Cuts at: 1524 1617 2673 1524
Size: 93 1056 2750
Fragments arranged by size:
2750 1056 93
BmgBI CAC'GTC
Cuts at: 165 165
Size: 3899
BmrI ACTGGGnnnn_n'
Cuts at: 1111 1192 1527 3671 1111
Size: 81 335 2144 1339
Fragments arranged by size:
2144 1339 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 668 2266 668
Size: 1598 2301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 568
Size: 3899
Bpu10I CC'TnA_GC
Cuts at: 445 463 643 445
Size: 18 180 3701
Fragments arranged by size:
3701 180 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1075 1954 2446 2744 1075
Size: 879 492 298 2230
Fragments arranged by size:
2230 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3603 3603
Size: 3899
BsaAI yAC'GTr
Cuts at: 1252 1252
Size: 3899
BsaBI GATnn'nnATC
Cuts at: 6 1413 3450 6
Size: 1407 2037 455
Fragments arranged by size:
2037 1407 455
BsaHI Gr'CG_yC
Cuts at: 677 1330 1578 2280 3776 677
Size: 653 248 702 1496 800
Fragments arranged by size:
1496 800 702 653 248
BsaWI w'CCGG_w
Cuts at: 500 1088 1609 2287 2561 2708 3119 500
Size: 588 521 678 274 147 411 1280
Fragments arranged by size:
1280 678 588 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 427 565 671 824 427
Size: 138 106 153 3502
Fragments arranged by size:
3502 153 138 106
BseYI C'CCAG_C
Cuts at: 294 692 1324 2082 2659 3155 294
Size: 398 632 758 577 496 1038
Fragments arranged by size:
1038 758 632 577 496 398
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 376 525 868 265
Size: 111 149 343 3296
Fragments arranged by size:
3296 343 149 111
BsiEI CG_ry'CG
Cuts at: 104 534 1441 1487 2695 104
Size: 430 907 46 1208 1308
Fragments arranged by size:
1308 1208 907 430 46
BsiHKAI G_wGCw'C
Cuts at: 913 1691 1881 2673 913
Size: 778 190 792 2139
Fragments arranged by size:
2139 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 913 1524 1617 1691 1881 1943 2673 913
Size: 611 93 74 190 62 730 2139
Fragments arranged by size:
2139 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 500 3119 500
Size: 2619 1280
BspHI T'CATG_A
Cuts at: 885 3075 885
Size: 2190 1709
BspMI ACCTGCnnnn'nnnn_
Cuts at: 949 1163 1465 1846 949
Size: 214 302 381 3002
Fragments arranged by size:
3002 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2191 2245 3729 3894 50
Size: 2141 54 1484 165 55
Fragments arranged by size:
2141 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1811 1811
Size: 3899
BsrFI r'CCGG_y
Cuts at: 183 1897 2078 2287 183
Size: 1714 181 209 1795
Fragments arranged by size:
1795 1714 209 181
BssSI C'ACGA_G
Cuts at: 2170 2528 2170
Size: 358 3541
BstAPI GCAn_nnn'nTGC
Cuts at: 1276 1276
Size: 3899
BstBI TT'CG_AA
Cuts at: 2260 2260
Size: 3899
BstXI CCAn_nnnn'nTGG
Cuts at: 754 754
Size: 3899
BstYI r'GATC_y
Cuts at: 1 590 791 924 1391 1414 1749 1995 3007
Size: 589 201 133 467 23 335 246 1012
Cuts at: 3007 3093 3105 3451 1
Size: 86 12 346 449
Fragments arranged by size:
1012 589 467 449 346 335 246 201
133 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 206 836 206
Size: 630 3269
BtgI C'CryG_G
Cuts at: 107 107
Size: 3899
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 867 1849 2030 2193 867
Size: 982 181 163 2573
Fragments arranged by size:
2573 982 181 163
BtsI GCAGTG_nn'
Cuts at: 1142 1142
Size: 3899
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 462 497 462
Size: 35 3864
DraI TTT'AAA
Cuts at: 898 898
Size: 3899
DraIII CAC_nnn'GTG
Cuts at: 825 825
Size: 3899
DrdI GACnn_nn'nnGTC
Cuts at: 1605 2463 1605
Size: 858 3041
EaeI y'GGCC_r
Cuts at: 212 531 816 1484 1658 2049 2076 3421 212
Size: 319 285 668 174 391 27 1345 690
Fragments arranged by size:
1345 690 668 391 319 285 174 27
EagI C'GGCC_G
Cuts at: 531 1484 531
Size: 953 2946
EarI CTCTTCn'nnn_
Cuts at: 1922 2132 1922
Size: 210 3689
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2107 2417 2563 3709 2107
Size: 310 146 1146 2297
Fragments arranged by size:
2297 1146 310 146
EcoICRI GAG'CTC
Cuts at: 911 911
Size: 3899
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 1724 2156 2903 3171 568
Size: 1156 432 747 268 1296
Fragments arranged by size:
1296 1156 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 409 724 409
Size: 315 3584
EcoO109I rG'GnC_Cy
Cuts at: 173 1038 173
Size: 865 3034
EcoRI G'AATT_C
Cuts at: 903 903
Size: 3899
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1918 1918
Size: 3899
FspI TGC'GCA
Cuts at: 1680 1680
Size: 3899
HaeII r_GCGC'y
Cuts at: 705 1333 1581 2603 3582 705
Size: 628 248 1022 979 1022
Fragments arranged by size:
1022 1022 979 628 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 233 265 362 394 650 682 1741 1771 1773
Size: 32 97 32 256 32 1059 30 2
Cuts at: 1773 1803 233
Size: 30 2329
Fragments arranged by size:
2329 1059 256 97 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 938 938
Size: 3899
KasI G'GCGC_C
Cuts at: 1329 1577 1329
Size: 248 3651
KpnI G_GTAC'C
Cuts at: 919 919
Size: 3899
MluI A'CGCG_T
Cuts at: 3332 3332
Size: 3899
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2570 2754 2570
Size: 184 3715
MscI TGG'CCA
Cuts at: 818 1660 818
Size: 842 3057
MslI CAynn'nnrTG
Cuts at: 1888 2015 1888
Size: 127 3772
MspA1I CmG'CkG
Cuts at: 294 1010 1324 1684 2697 2942 294
Size: 716 314 360 1013 245 1251
Fragments arranged by size:
1251 1013 716 360 314 245
NaeI GCC'GGC
Cuts at: 185 2080 185
Size: 1895 2004
NarI GG'CG_CC
Cuts at: 1330 1578 1330
Size: 248 3651
NcoI C'CATG_G
Cuts at: 107 107
Size: 3899
NgoMIV G'CCGG_C
Cuts at: 183 2078 183
Size: 1895 2004
NspI r_CATG'y
Cuts at: 513 952 1452 2359 513
Size: 439 500 907 2053
Fragments arranged by size:
2053 907 500 439
PciI A'CATG_T
Cuts at: 2355 2355
Size: 3899
PflMI CCAn_nnn'nTGG
Cuts at: 825 825
Size: 3899
PfoI T'CCnGG_A
Cuts at: 593 593
Size: 3899
PmeI GTTT'AAAC
Cuts at: 898 898
Size: 3899
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3038 3070 3038
Size: 32 3867
PpuMI rG'GwC_Cy
Cuts at: 173 173
Size: 3899
PstI C_TGCA'G
Cuts at: 227 425 946 1631 227
Size: 198 521 685 2495
Fragments arranged by size:
2495 685 521 198
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3899
PvuII CAG'CTG
Cuts at: 294 1324 1684 294
Size: 1030 360 2509
Fragments arranged by size:
2509 1030 360
RsrII CG'GwC_CG
Cuts at: 2094 2094
Size: 3899
SacI G_AGCT'C
Cuts at: 913 913
Size: 3899
SalI G'TCGA_C
Cuts at: 936 936
Size: 3899
SanDI GG'GwC_CC
Cuts at: 173 173
Size: 3899
SapI GCTCTTCn'nnn_
Cuts at: 1922 2132 1922
Size: 210 3689
SbfI CC_TGCA'GG
Cuts at: 946 946
Size: 3899
ScaI AGT'ACT
Cuts at: 3416 3416
Size: 3899
SfcI C'TryA_G
Cuts at: 33 223 421 942 1627 2620 2811 33
Size: 190 198 521 685 993 191 1121
Fragments arranged by size:
1121 993 685 521 198 191 190
SfoI GGC'GCC
Cuts at: 1331 1579 1331
Size: 248 3651
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3899
SmaI CCC'GGG
Cuts at: 921 921
Size: 3899
SmlI C'TyrA_G
Cuts at: 653 1054 1969 2461 2723 653
Size: 401 915 492 262 1829
Fragments arranged by size:
1829 915 492 401 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3899
SphI G_CATG'C
Cuts at: 513 952 1452 513
Size: 439 500 2960
Fragments arranged by size:
2960 500 439
StyI C'CwwG_G
Cuts at: 107 1033 1387 107
Size: 926 354 2619
Fragments arranged by size:
2619 926 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 286 1482 1615 2074 2283 286
Size: 1196 133 459 209 1902
Fragments arranged by size:
1902 1196 459 209 133
TatI w'GTAC_w
Cuts at: 3414 3414
Size: 3899
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3416 3448 3416
Size: 32 3867
Tth111I GACn'n_nGTC
Cuts at: 1696 1696
Size: 3899
XbaI T'CTAG_A
Cuts at: 930 930
Size: 3899
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1394 1394
Size: 3899
XhoI C'TCGA_G
Cuts at: 653 653
Size: 3899
XmaI C'CCGG_G
Cuts at: 919 919
Size: 3899
ZraI GAC'GTC
Cuts at: 678 678
Size: 3899
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmtI BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XmnI