Restriction map for
pF1KE0535
(Circular) (Six-base) MAPSORT of: pF1KE0535.seq Check: 4666 from: 1 to: 4340
pF1KE0535 4340 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 693 693
Size: 4340
AatII G_ACGT'C
Cuts at: 118 118
Size: 4340
AccI GT'mk_AC
Cuts at: 524 1378 524
Size: 854 3486
Acc65I G'GTAC_C
Cuts at: 344 1356 344
Size: 1012 3328
AclI AA'CG_TT
Cuts at: 4180 4180
Size: 4340
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 1146 2165 2597 3344 3612 646
Size: 500 1019 432 747 268 1374
Fragments arranged by size:
1374 1019 747 500 432 268
AflIII A'CryG_T
Cuts at: 1692 2796 3773 1692
Size: 1104 977 2259
Fragments arranged by size:
2259 1104 977
AgeI A'CCGG_T
Cuts at: 2728 2728
Size: 4340
AleI CACnn'nnGTG
Cuts at: 1081 1246 1081
Size: 165 4175
AlwNI CAG_nnn'CTG
Cuts at: 1645 3212 1645
Size: 1567 2773
ApaI G_GGCC'C
Cuts at: 769 769
Size: 4340
ApaLI G'TGCA_C
Cuts at: 777 3110 777
Size: 2333 2007
ApoI r'AATT_y
Cuts at: 1344 3693 3949 1344
Size: 2349 256 1735
Fragments arranged by size:
2349 1735 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4340
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4340
AvaI C'yCGr_G
Cuts at: 842 1360 842
Size: 518 3822
BamHI G'GATC_C
Cuts at: 686 1365 686
Size: 679 3661
BanI G'GyrC_C
Cuts at: 125 344 938 1356 1770 2018 2053 125
Size: 219 594 418 414 248 35 2412
Fragments arranged by size:
2412 594 418 414 248 219 35
BanII G_rGCy'C
Cuts at: 769 860 1001 1090 1150 1240 1330 1354 2384
Size: 91 141 89 60 90 90 24 1030
Cuts at: 2384 769
Size: 2725
Fragments arranged by size:
2725 1030 141 91 90 90 89 60
24
BbeI G_GCGC'C
Cuts at: 942 1774 2022 942
Size: 832 248 3260
Fragments arranged by size:
3260 832 248
BbvCI CC'TCA_GC
Cuts at: 601 601
Size: 4340
BciVI GTATCCnnnnn_n'
Cuts at: 2238 2999 4340 2238
Size: 761 1341 2238
Fragments arranged by size:
2238 1341 761
BclI T'GATC_A
Cuts at: 1860 1860
Size: 4340
BglI GCCn_nnn'nGGC
Cuts at: 507 507
Size: 4340
BglII A'GATC_T
Cuts at: 1855 3892 1855
Size: 2037 2303
BlpI GC'TnA_GC
Cuts at: 1452 1452
Size: 4340
Bme1580I G_kGCm'C
Cuts at: 128 769 781 1965 2058 3114 128
Size: 641 12 1184 93 1056 1354
Fragments arranged by size:
1354 1184 1056 641 93 12
BmrI ACTGGGnnnn_n'
Cuts at: 432 1552 1633 1968 4112 432
Size: 1120 81 335 2144 660
Fragments arranged by size:
2144 1120 660 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2707 2707
Size: 4340
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 772 400
Size: 372 3968
Bpu10I CC'TnA_GC
Cuts at: 199 601 199
Size: 402 3938
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1516 2395 2887 3185 1516
Size: 879 492 298 2671
Fragments arranged by size:
2671 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4044 4044
Size: 4340
BsaAI yAC'GTr
Cuts at: 1693 1693
Size: 4340
BsaBI GATnn'nnATC
Cuts at: 6 1854 3891 6
Size: 1848 2037 455
Fragments arranged by size:
2037 1848 455
BsaHI Gr'CG_yC
Cuts at: 115 939 1193 1771 2019 2721 4217 115
Size: 824 254 578 248 702 1496 238
Fragments arranged by size:
1496 824 702 578 254 248 238
BsaWI w'CCGG_w
Cuts at: 1529 2050 2728 3002 3149 3560 1529
Size: 521 678 274 147 411 2309
Fragments arranged by size:
2309 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1072 1102 1222 1252 1072
Size: 30 120 30 4160
Fragments arranged by size:
4160 120 30 30
BseYI C'CCAG_C
Cuts at: 387 1765 2523 3100 3596 387
Size: 1378 758 577 496 1131
Fragments arranged by size:
1378 1131 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 130 640 1102 130
Size: 510 462 3368
Fragments arranged by size:
3368 510 462
BsiEI CG_ry'CG
Cuts at: 104 1882 1928 3136 104
Size: 1778 46 1208 1308
Fragments arranged by size:
1778 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 781 860 1001 1217 1253 1354 2132 2322 3114
Size: 79 141 216 36 101 778 190 792
Cuts at: 3114 781
Size: 2007
Fragments arranged by size:
2007 792 778 216 190 141 101 79
36
Bsp1286I G_dGCh'C
Cuts at: 128 769 781 860 1001 1090 1150 1217 1240
Size: 641 12 79 141 89 60 67 23
Cuts at: 1240 1253 1330 1354 1965 2058 2132 2322 2384
Size: 13 77 24 611 93 74 190 62
Cuts at: 2384 3114 128
Size: 730 1354
Fragments arranged by size:
1354 730 641 611 190 141 93 89
79 77 74 67 62 60 24 23
13 12
BspEI T'CCGG_A
Cuts at: 3560 3560
Size: 4340
BspHI T'CATG_A
Cuts at: 3516 3516
Size: 4340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 590 693 717 797 1124 1390 1604 1906 2287
Size: 103 24 80 327 266 214 302 381
Cuts at: 2287 590
Size: 2643
Fragments arranged by size:
2643 381 327 302 266 214 103 80
24
BsrBI CCG'CTC
Cuts at: 50 1017 2632 2686 4170 4335 50
Size: 967 1615 54 1484 165 55
Fragments arranged by size:
1615 1484 967 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2252 2252
Size: 4340
BsrFI r'CCGG_y
Cuts at: 347 935 2338 2519 2728 347
Size: 588 1403 181 209 1959
Fragments arranged by size:
1959 1403 588 209 181
BsrGI T'GTAC_A
Cuts at: 437 533 437
Size: 96 4244
BssSI C'ACGA_G
Cuts at: 2611 2969 2611
Size: 358 3982
BstAPI GCAn_nnn'nTGC
Cuts at: 1717 1717
Size: 4340
BstBI TT'CG_AA
Cuts at: 2701 2701
Size: 4340
BstEII G'GTnAC_C
Cuts at: 738 837 738
Size: 99 4241
BstXI CCAn_nnnn'nTGG
Cuts at: 318 1083 318
Size: 765 3575
BstYI r'GATC_y
Cuts at: 1 686 1365 1832 1855 2190 2436 3448 3534
Size: 685 679 467 23 335 246 1012 86
Cuts at: 3534 3546 3892 1
Size: 12 346 449
Fragments arranged by size:
1012 685 679 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 119 160 107
Size: 12 41 4287
Fragments arranged by size:
4287 41 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2290 2471 2634 2290
Size: 181 163 3996
Fragments arranged by size:
3996 181 163
BtsI GCAGTG_nn'
Cuts at: 191 269 897 1583 191
Size: 78 628 686 2948
Fragments arranged by size:
2948 686 628 78
DraI TTT'AAA
Cuts at: 1339 1339
Size: 4340
DraIII CAC_nnn'GTG
Cuts at: 663 663
Size: 4340
DrdI GACnn_nn'nnGTC
Cuts at: 2046 2904 2046
Size: 858 3482
EaeI y'GGCC_r
Cuts at: 1925 2099 2490 2517 3862 1925
Size: 174 391 27 1345 2403
Fragments arranged by size:
2403 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1925 1925
Size: 4340
EarI CTCTTCn'nnn_
Cuts at: 1006 2363 2573 1006
Size: 1357 210 2773
Fragments arranged by size:
2773 1357 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 180 2548 2858 3004 4150 180
Size: 2368 310 146 1146 370
Fragments arranged by size:
2368 1146 370 310 146
EcoICRI GAG'CTC
Cuts at: 858 999 1352 858
Size: 141 353 3846
Fragments arranged by size:
3846 353 141
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 646 772 1146 2165 2597 3344 3612 400
Size: 246 126 374 1019 432 747 268 1128
Fragments arranged by size:
1128 1019 747 432 374 268 246 126
EcoNI CCTnn'n_nnAGG
Cuts at: 487 487
Size: 4340
EcoO109I rG'GnC_Cy
Cuts at: 579 786 1113 1263 1479 579
Size: 207 327 150 216 3440
Fragments arranged by size:
3440 327 216 207 150
EcoRI G'AATT_C
Cuts at: 1344 1344
Size: 4340
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2359 2359
Size: 4340
FspI TGC'GCA
Cuts at: 514 2121 514
Size: 1607 2733
HaeII r_GCGC'y
Cuts at: 942 1774 2022 3044 4023 942
Size: 832 248 1022 979 1259
Fragments arranged by size:
1259 1022 979 832 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1178 1210 1289 1321 2182 2212 2214 2244 1178
Size: 32 79 32 861 30 2 30 3274
Fragments arranged by size:
3274 861 79 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1379 1379
Size: 4340
KasI G'GCGC_C
Cuts at: 938 1770 2018 938
Size: 832 248 3260
Fragments arranged by size:
3260 832 248
KpnI G_GTAC'C
Cuts at: 348 1360 348
Size: 1012 3328
MluI A'CGCG_T
Cuts at: 3773 3773
Size: 4340
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3011 3195 3011
Size: 184 4156
MscI TGG'CCA
Cuts at: 2101 2101
Size: 4340
MslI CAynn'nnrTG
Cuts at: 182 1081 1246 2329 2456 182
Size: 899 165 1083 127 2066
Fragments arranged by size:
2066 1083 899 165 127
MspA1I CmG'CkG
Cuts at: 571 834 1066 1287 1451 1765 2125 3138 3383
Size: 263 232 221 164 314 360 1013 245
Cuts at: 3383 571
Size: 1528
Fragments arranged by size:
1528 1013 360 314 263 245 232 221
164
NaeI GCC'GGC
Cuts at: 2521 2521
Size: 4340
NarI GG'CG_CC
Cuts at: 939 1771 2019 939
Size: 832 248 3260
Fragments arranged by size:
3260 832 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4340
NgoMIV G'CCGG_C
Cuts at: 2519 2519
Size: 4340
NspI r_CATG'y
Cuts at: 1393 1893 2800 1393
Size: 500 907 2933
Fragments arranged by size:
2933 907 500
PasI CC'CwG_GG
Cuts at: 886 1323 886
Size: 437 3903
PciI A'CATG_T
Cuts at: 2796 2796
Size: 4340
PflMI CCAn_nnn'nTGG
Cuts at: 679 920 679
Size: 241 4099
PmeI GTTT'AAAC
Cuts at: 1339 1339
Size: 4340
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3479 3511 3479
Size: 32 4308
PpuMI rG'GwC_Cy
Cuts at: 579 786 1113 579
Size: 207 327 3806
Fragments arranged by size:
3806 327 207
PspOMI G'GGCC_C
Cuts at: 765 765
Size: 4340
PstI C_TGCA'G
Cuts at: 1387 2072 1387
Size: 685 3655
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4340
PvuII CAG'CTG
Cuts at: 834 1066 1287 1765 2125 834
Size: 232 221 478 360 3049
Fragments arranged by size:
3049 478 360 232 221
RsrII CG'GwC_CG
Cuts at: 2535 2535
Size: 4340
SacI G_AGCT'C
Cuts at: 860 1001 1354 860
Size: 141 353 3846
Fragments arranged by size:
3846 353 141
SalI G'TCGA_C
Cuts at: 1377 1377
Size: 4340
SapI GCTCTTCn'nnn_
Cuts at: 1006 2363 2573 1006
Size: 1357 210 2773
Fragments arranged by size:
2773 1357 210
SbfI CC_TGCA'GG
Cuts at: 1387 1387
Size: 4340
ScaI AGT'ACT
Cuts at: 3857 3857
Size: 4340
SfcI C'TryA_G
Cuts at: 33 211 525 1383 2068 3061 3252 33
Size: 178 314 858 685 993 191 1121
Fragments arranged by size:
1121 993 858 685 314 191 178
SfoI GGC'GCC
Cuts at: 940 1772 2020 940
Size: 832 248 3260
Fragments arranged by size:
3260 832 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4340
SgrAI Cr'CCGG_yG
Cuts at: 935 935
Size: 4340
SmaI CCC'GGG
Cuts at: 844 1362 844
Size: 518 3822
SmlI C'TyrA_G
Cuts at: 1495 2410 2902 3164 1495
Size: 915 492 262 2671
Fragments arranged by size:
2671 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4340
SphI G_CATG'C
Cuts at: 1393 1893 1393
Size: 500 3840
StyI C'CwwG_G
Cuts at: 107 1132 1268 1474 1828 107
Size: 1025 136 206 354 2619
Fragments arranged by size:
2619 1025 354 206 136
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 420 1923 2056 2515 2724 420
Size: 1503 133 459 209 2036
Fragments arranged by size:
2036 1503 459 209 133
TatI w'GTAC_w
Cuts at: 362 437 533 3855 362
Size: 75 96 3322 847
Fragments arranged by size:
3322 847 96 75
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3857 3889 3857
Size: 32 4308
Tth111I GACn'n_nGTC
Cuts at: 927 2137 927
Size: 1210 3130
XbaI T'CTAG_A
Cuts at: 1371 1371
Size: 4340
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1835 1835
Size: 4340
XmaI C'CCGG_G
Cuts at: 842 1360 842
Size: 518 3822
ZraI GAC'GTC
Cuts at: 116 116
Size: 4340
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AloI AscI AvrII BaeI
BbsI BcgI BfrBI BmgBI BmtI BseRI BsiWI BsmI
BsmBI BssHII BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SnaBI SrfI SspI StuI
SwaI XhoI XmnI