Restriction map for
pF1KE0532
(Circular) (Six-base) MAPSORT of: pF1KE0532.seq Check: 7065 from: 1 to: 3575
pF1KE0532 3575 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 613 613
Size: 3575
Acc65I G'GTAC_C
Cuts at: 591 591
Size: 3575
AclI AA'CG_TT
Cuts at: 3415 3415
Size: 3575
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1400 1832 2579 2847 1400
Size: 432 747 268 2128
Fragments arranged by size:
2128 747 432 268
AflIII A'CryG_T
Cuts at: 489 927 2031 3008 489
Size: 438 1104 977 1056
Fragments arranged by size:
1104 1056 977 438
AgeI A'CCGG_T
Cuts at: 1963 1963
Size: 3575
AleI CACnn'nnGTG
Cuts at: 232 232
Size: 3575
AlwNI CAG_nnn'CTG
Cuts at: 880 2447 880
Size: 1567 2008
ApaLI G'TGCA_C
Cuts at: 325 2345 325
Size: 2020 1555
ApoI r'AATT_y
Cuts at: 579 2928 3184 579
Size: 2349 256 970
Fragments arranged by size:
2349 970 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3575
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3575
AvaI C'yCGr_G
Cuts at: 595 595
Size: 3575
BamHI G'GATC_C
Cuts at: 600 600
Size: 3575
BanI G'GyrC_C
Cuts at: 312 591 1005 1253 1288 312
Size: 279 414 248 35 2599
Fragments arranged by size:
2599 414 279 248 35
BanII G_rGCy'C
Cuts at: 290 589 1619 290
Size: 299 1030 2246
Fragments arranged by size:
2246 1030 299
BbeI G_GCGC'C
Cuts at: 1009 1257 1009
Size: 248 3327
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 306 340 306
Size: 34 3541
BciVI GTATCCnnnnn_n'
Cuts at: 1473 2234 3575 1473
Size: 761 1341 1473
Fragments arranged by size:
1473 1341 761
BclI T'GATC_A
Cuts at: 1095 1095
Size: 3575
BglII A'GATC_T
Cuts at: 1090 3127 1090
Size: 2037 1538
BlpI GC'TnA_GC
Cuts at: 687 687
Size: 3575
Bme1580I G_kGCm'C
Cuts at: 329 1200 1293 2349 329
Size: 871 93 1056 1555
Fragments arranged by size:
1555 1056 871 93
BmrI ACTGGGnnnn_n'
Cuts at: 787 868 1203 3347 787
Size: 81 335 2144 1015
Fragments arranged by size:
2144 1015 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1942 1942
Size: 3575
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 1630 2122 2420 751
Size: 879 492 298 1906
Fragments arranged by size:
1906 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3279 3279
Size: 3575
BsaAI yAC'GTr
Cuts at: 928 928
Size: 3575
BsaBI GATnn'nnATC
Cuts at: 6 1089 3126 6
Size: 1083 2037 455
Fragments arranged by size:
2037 1083 455
BsaHI Gr'CG_yC
Cuts at: 200 1006 1254 1956 3452 200
Size: 806 248 702 1496 323
Fragments arranged by size:
1496 806 702 323 248
BsaWI w'CCGG_w
Cuts at: 764 1285 1963 2237 2384 2795 764
Size: 521 678 274 147 411 1544
Fragments arranged by size:
1544 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1000 1758 2335 2831 1000
Size: 758 577 496 1744
Fragments arranged by size:
1744 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1117 1163 2371 104
Size: 1013 46 1208 1308
Fragments arranged by size:
1308 1208 1013 46
BsiHKAI G_wGCw'C
Cuts at: 290 329 589 1367 1557 2349 290
Size: 39 260 778 190 792 1516
Fragments arranged by size:
1516 792 778 260 190 39
BsmI GAATG_Cn'
Cuts at: 399 399
Size: 3575
Bsp1286I G_dGCh'C
Cuts at: 290 329 589 1200 1293 1367 1557 1619 2349
Size: 39 260 611 93 74 190 62 730
Cuts at: 2349 290
Size: 1516
Fragments arranged by size:
1516 730 611 260 190 93 74 62
39
BspEI T'CCGG_A
Cuts at: 2795 2795
Size: 3575
BspHI T'CATG_A
Cuts at: 2751 2751
Size: 3575
BspMI ACCTGCnnnn'nnnn_
Cuts at: 625 839 1141 1522 625
Size: 214 302 381 2678
Fragments arranged by size:
2678 381 302 214
BsrBI CCG'CTC
Cuts at: 50 197 1867 1921 3405 3570 50
Size: 147 1670 54 1484 165 55
Fragments arranged by size:
1670 1484 165 147 55 54
BsrDI GCAATG_nn'
Cuts at: 1487 1487
Size: 3575
BsrFI r'CCGG_y
Cuts at: 1573 1754 1963 1573
Size: 181 209 3185
Fragments arranged by size:
3185 209 181
BssSI C'ACGA_G
Cuts at: 1846 2204 1846
Size: 358 3217
BstAPI GCAn_nnn'nTGC
Cuts at: 142 952 142
Size: 810 2765
BstBI TT'CG_AA
Cuts at: 1936 1936
Size: 3575
BstYI r'GATC_y
Cuts at: 1 600 1067 1090 1425 1671 2683 2769 2781
Size: 599 467 23 335 246 1012 86 12
Cuts at: 2781 3127 1
Size: 346 449
Fragments arranged by size:
1012 599 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 127 107
Size: 20 3555
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1525 1706 1869 1525
Size: 181 163 3231
Fragments arranged by size:
3231 181 163
BtsI GCAGTG_nn'
Cuts at: 818 818
Size: 3575
DraI TTT'AAA
Cuts at: 574 574
Size: 3575
DrdI GACnn_nn'nnGTC
Cuts at: 1281 2139 1281
Size: 858 2717
EaeI y'GGCC_r
Cuts at: 558 1160 1334 1725 1752 3097 558
Size: 602 174 391 27 1345 1036
Fragments arranged by size:
1345 1036 602 391 174 27
EagI C'GGCC_G
Cuts at: 1160 1160
Size: 3575
EarI CTCTTCn'nnn_
Cuts at: 1598 1808 1598
Size: 210 3365
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 193 1783 2093 2239 3385 193
Size: 1590 310 146 1146 383
Fragments arranged by size:
1590 1146 383 310 146
EcoICRI GAG'CTC
Cuts at: 288 587 288
Size: 299 3276
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1400 1832 2579 2847 1400
Size: 432 747 268 2128
Fragments arranged by size:
2128 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 714 714
Size: 3575
EcoRI G'AATT_C
Cuts at: 579 579
Size: 3575
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1594 1594
Size: 3575
FspI TGC'GCA
Cuts at: 1356 1356
Size: 3575
HaeII r_GCGC'y
Cuts at: 523 1009 1257 2279 3258 523
Size: 486 248 1022 979 840
Fragments arranged by size:
1022 979 840 486 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 526 558 1417 1447 1449 1479 526
Size: 32 859 30 2 30 2622
Fragments arranged by size:
2622 859 32 30 30 2
HincII GTy'rAC
Cuts at: 614 614
Size: 3575
KasI G'GCGC_C
Cuts at: 1005 1253 1005
Size: 248 3327
KpnI G_GTAC'C
Cuts at: 595 595
Size: 3575
MluI A'CGCG_T
Cuts at: 3008 3008
Size: 3575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2246 2430 2246
Size: 184 3391
MscI TGG'CCA
Cuts at: 560 1336 560
Size: 776 2799
MslI CAynn'nnrTG
Cuts at: 232 349 1564 1691 232
Size: 117 1215 127 2116
Fragments arranged by size:
2116 1215 127 117
MspA1I CmG'CkG
Cuts at: 129 339 483 686 1000 1360 2373 2618 129
Size: 210 144 203 314 360 1013 245 1086
Fragments arranged by size:
1086 1013 360 314 245 210 203 144
NaeI GCC'GGC
Cuts at: 1756 1756
Size: 3575
NarI GG'CG_CC
Cuts at: 1006 1254 1006
Size: 248 3327
NcoI C'CATG_G
Cuts at: 107 107
Size: 3575
NgoMIV G'CCGG_C
Cuts at: 1754 1754
Size: 3575
NspI r_CATG'y
Cuts at: 493 628 1128 2035 493
Size: 135 500 907 2033
Fragments arranged by size:
2033 907 500 135
PasI CC'CwG_GG
Cuts at: 413 413
Size: 3575
PciI A'CATG_T
Cuts at: 489 2031 489
Size: 1542 2033
PmeI GTTT'AAAC
Cuts at: 574 574
Size: 3575
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2714 2746 2714
Size: 32 3543
PstI C_TGCA'G
Cuts at: 622 1307 622
Size: 685 2890
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3575
PvuII CAG'CTG
Cuts at: 339 1000 1360 339
Size: 661 360 2554
Fragments arranged by size:
2554 661 360
RsrII CG'GwC_CG
Cuts at: 131 1770 131
Size: 1639 1936
SacI G_AGCT'C
Cuts at: 290 589 290
Size: 299 3276
SacII CC_GC'GG
Cuts at: 130 130
Size: 3575
SalI G'TCGA_C
Cuts at: 612 612
Size: 3575
SapI GCTCTTCn'nnn_
Cuts at: 1598 1808 1598
Size: 210 3365
SbfI CC_TGCA'GG
Cuts at: 622 622
Size: 3575
ScaI AGT'ACT
Cuts at: 3092 3092
Size: 3575
SfcI C'TryA_G
Cuts at: 33 618 1303 2296 2487 33
Size: 585 685 993 191 1121
Fragments arranged by size:
1121 993 685 585 191
SfoI GGC'GCC
Cuts at: 1007 1255 1007
Size: 248 3327
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3575
SmaI CCC'GGG
Cuts at: 597 597
Size: 3575
SmlI C'TyrA_G
Cuts at: 730 1645 2137 2399 730
Size: 915 492 262 1906
Fragments arranged by size:
1906 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3575
SphI G_CATG'C
Cuts at: 628 1128 628
Size: 500 3075
StyI C'CwwG_G
Cuts at: 107 523 709 1063 107
Size: 416 186 354 2619
Fragments arranged by size:
2619 416 354 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1158 1291 1750 1959 1158
Size: 133 459 209 2774
Fragments arranged by size:
2774 459 209 133
TatI w'GTAC_w
Cuts at: 3090 3090
Size: 3575
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3092 3124 3092
Size: 32 3543
Tth111I GACn'n_nGTC
Cuts at: 1372 1372
Size: 3575
XbaI T'CTAG_A
Cuts at: 606 606
Size: 3575
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1070 1070
Size: 3575
XmaI C'CCGG_G
Cuts at: 595 595
Size: 3575
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI