Restriction map for
pF1KE0530
(Circular) (Six-base) MAPSORT of: pF1KE0530.seq Check: 1749 from: 1 to: 3575
pF1KE0530 3575 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 613 613
Size: 3575
Acc65I G'GTAC_C
Cuts at: 591 591
Size: 3575
AclI AA'CG_TT
Cuts at: 3415 3415
Size: 3575
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 1400 1832 2579 2847 277
Size: 1123 432 747 268 1005
Fragments arranged by size:
1123 1005 747 432 268
AflII C'TTAA_G
Cuts at: 337 337
Size: 3575
AflIII A'CryG_T
Cuts at: 927 2031 3008 927
Size: 1104 977 1494
Fragments arranged by size:
1494 1104 977
AgeI A'CCGG_T
Cuts at: 1963 1963
Size: 3575
AlwNI CAG_nnn'CTG
Cuts at: 453 880 2447 453
Size: 427 1567 1581
Fragments arranged by size:
1581 1567 427
ApaLI G'TGCA_C
Cuts at: 2345 2345
Size: 3575
ApoI r'AATT_y
Cuts at: 579 2928 3184 579
Size: 2349 256 970
Fragments arranged by size:
2349 970 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3575
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3575
AvaI C'yCGr_G
Cuts at: 469 595 469
Size: 126 3449
BamHI G'GATC_C
Cuts at: 600 600
Size: 3575
BanI G'GyrC_C
Cuts at: 304 591 1005 1253 1288 304
Size: 287 414 248 35 2591
Fragments arranged by size:
2591 414 287 248 35
BanII G_rGCy'C
Cuts at: 116 589 1619 116
Size: 473 1030 2072
Fragments arranged by size:
2072 1030 473
BbeI G_GCGC'C
Cuts at: 1009 1257 1009
Size: 248 3327
BciVI GTATCCnnnnn_n'
Cuts at: 1473 2234 3575 1473
Size: 761 1341 1473
Fragments arranged by size:
1473 1341 761
BclI T'GATC_A
Cuts at: 1095 1095
Size: 3575
BfrBI ATG'CAT
Cuts at: 440 440
Size: 3575
BglI GCCn_nnn'nGGC
Cuts at: 496 496
Size: 3575
BglII A'GATC_T
Cuts at: 1090 3127 1090
Size: 2037 1538
BlpI GC'TnA_GC
Cuts at: 115 687 115
Size: 572 3003
Bme1580I G_kGCm'C
Cuts at: 1200 1293 2349 1200
Size: 93 1056 2426
Fragments arranged by size:
2426 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 787 868 1203 3347 787
Size: 81 335 2144 1015
Fragments arranged by size:
2144 1015 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 447 1942 447
Size: 1495 2080
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 442 535 250
Size: 192 93 3290
Fragments arranged by size:
3290 192 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 1630 2122 2420 751
Size: 879 492 298 1906
Fragments arranged by size:
1906 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3279 3279
Size: 3575
BsaAI yAC'GTr
Cuts at: 928 928
Size: 3575
BsaBI GATnn'nnATC
Cuts at: 6 1089 3126 6
Size: 1083 2037 455
Fragments arranged by size:
2037 1083 455
BsaHI Gr'CG_yC
Cuts at: 1006 1254 1956 3452 1006
Size: 248 702 1496 1129
Fragments arranged by size:
1496 1129 702 248
BsaWI w'CCGG_w
Cuts at: 524 764 1285 1963 2237 2384 2795 524
Size: 240 521 678 274 147 411 1304
Fragments arranged by size:
1304 678 521 411 274 240 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 523 553 523
Size: 30 3545
BseYI C'CCAG_C
Cuts at: 555 1000 1758 2335 2831 555
Size: 445 758 577 496 1299
Fragments arranged by size:
1299 758 577 496 445
BsiEI CG_ry'CG
Cuts at: 104 1117 1163 2371 104
Size: 1013 46 1208 1308
Fragments arranged by size:
1308 1208 1013 46
BsiHKAI G_wGCw'C
Cuts at: 317 589 1367 1557 2349 317
Size: 272 778 190 792 1543
Fragments arranged by size:
1543 792 778 272 190
BsmI GAATG_Cn'
Cuts at: 442 442
Size: 3575
Bsp1286I G_dGCh'C
Cuts at: 116 317 589 1200 1293 1367 1557 1619 2349
Size: 201 272 611 93 74 190 62 730
Cuts at: 2349 116
Size: 1342
Fragments arranged by size:
1342 730 611 272 201 190 93 74
62
BspEI T'CCGG_A
Cuts at: 524 2795 524
Size: 2271 1304
BspHI T'CATG_A
Cuts at: 354 2751 354
Size: 2397 1178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 625 839 1141 1522 625
Size: 214 302 381 2678
Fragments arranged by size:
2678 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1867 1921 3405 3570 50
Size: 1817 54 1484 165 55
Fragments arranged by size:
1817 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1487 1487
Size: 3575
BsrFI r'CCGG_y
Cuts at: 1573 1754 1963 1573
Size: 181 209 3185
Fragments arranged by size:
3185 209 181
BssSI C'ACGA_G
Cuts at: 1846 2204 1846
Size: 358 3217
BstAPI GCAn_nnn'nTGC
Cuts at: 138 952 138
Size: 814 2761
BstBI TT'CG_AA
Cuts at: 1936 1936
Size: 3575
BstEII G'GTnAC_C
Cuts at: 214 214
Size: 3575
BstYI r'GATC_y
Cuts at: 1 404 600 1067 1090 1425 1671 2683 2769
Size: 403 196 467 23 335 246 1012 86
Cuts at: 2769 2781 3127 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 403 346 335 246 196
86 23 12
BtgI C'CryG_G
Cuts at: 107 180 107
Size: 73 3502
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1525 1706 1869 1525
Size: 181 163 3231
Fragments arranged by size:
3231 181 163
BtsI GCAGTG_nn'
Cuts at: 818 818
Size: 3575
DraI TTT'AAA
Cuts at: 574 574
Size: 3575
DrdI GACnn_nn'nnGTC
Cuts at: 1281 2139 1281
Size: 858 2717
EaeI y'GGCC_r
Cuts at: 1160 1334 1725 1752 3097 1160
Size: 174 391 27 1345 1638
Fragments arranged by size:
1638 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1160 1160
Size: 3575
EarI CTCTTCn'nnn_
Cuts at: 1598 1808 1598
Size: 210 3365
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1783 2093 2239 3385 1783
Size: 310 146 1146 1973
Fragments arranged by size:
1973 1146 310 146
EcoICRI GAG'CTC
Cuts at: 587 587
Size: 3575
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 277 442 535 1400 1832 2579 2847 250
Size: 27 165 93 865 432 747 268 978
Fragments arranged by size:
978 865 747 432 268 165 93 27
EcoO109I rG'GnC_Cy
Cuts at: 510 714 510
Size: 204 3371
EcoRI G'AATT_C
Cuts at: 579 579
Size: 3575
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1594 1594
Size: 3575
FspI TGC'GCA
Cuts at: 1356 1356
Size: 3575
HaeII r_GCGC'y
Cuts at: 1009 1257 2279 3258 1009
Size: 248 1022 979 1326
Fragments arranged by size:
1326 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 450 482 523 555 1417 1447 1449 1479 450
Size: 32 41 32 862 30 2 30 2546
Fragments arranged by size:
2546 862 41 32 32 30 30 2
HincII GTy'rAC
Cuts at: 614 614
Size: 3575
KasI G'GCGC_C
Cuts at: 1005 1253 1005
Size: 248 3327
KpnI G_GTAC'C
Cuts at: 595 595
Size: 3575
MluI A'CGCG_T
Cuts at: 3008 3008
Size: 3575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 566 2246 2430 566
Size: 1680 184 1711
Fragments arranged by size:
1711 1680 184
MscI TGG'CCA
Cuts at: 1336 1336
Size: 3575
MslI CAynn'nnrTG
Cuts at: 1564 1691 1564
Size: 127 3448
MspA1I CmG'CkG
Cuts at: 686 1000 1360 2373 2618 686
Size: 314 360 1013 245 1643
Fragments arranged by size:
1643 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1756 1756
Size: 3575
NarI GG'CG_CC
Cuts at: 1006 1254 1006
Size: 248 3327
NcoI C'CATG_G
Cuts at: 107 180 107
Size: 73 3502
NgoMIV G'CCGG_C
Cuts at: 1754 1754
Size: 3575
NsiI A_TGCA'T
Cuts at: 442 442
Size: 3575
NspI r_CATG'y
Cuts at: 391 628 1128 2035 391
Size: 237 500 907 1931
Fragments arranged by size:
1931 907 500 237
PasI CC'CwG_GG
Cuts at: 324 492 324
Size: 168 3407
PciI A'CATG_T
Cuts at: 2031 2031
Size: 3575
PflMI CCAn_nnn'nTGG
Cuts at: 181 181
Size: 3575
PmeI GTTT'AAAC
Cuts at: 574 574
Size: 3575
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2714 2746 2714
Size: 32 3543
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 409 441 409
Size: 32 3543
PstI C_TGCA'G
Cuts at: 458 622 1307 458
Size: 164 685 2726
Fragments arranged by size:
2726 685 164
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3575
PvuII CAG'CTG
Cuts at: 1000 1360 1000
Size: 360 3215
RsrII CG'GwC_CG
Cuts at: 1770 1770
Size: 3575
SacI G_AGCT'C
Cuts at: 589 589
Size: 3575
SalI G'TCGA_C
Cuts at: 612 612
Size: 3575
SapI GCTCTTCn'nnn_
Cuts at: 1598 1808 1598
Size: 210 3365
SbfI CC_TGCA'GG
Cuts at: 622 622
Size: 3575
ScaI AGT'ACT
Cuts at: 3092 3092
Size: 3575
SfcI C'TryA_G
Cuts at: 33 454 618 1303 2296 2487 33
Size: 421 164 685 993 191 1121
Fragments arranged by size:
1121 993 685 421 191 164
SfoI GGC'GCC
Cuts at: 1007 1255 1007
Size: 248 3327
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3575
SmaI CCC'GGG
Cuts at: 471 597 471
Size: 126 3449
SmlI C'TyrA_G
Cuts at: 337 730 1645 2137 2399 337
Size: 393 915 492 262 1513
Fragments arranged by size:
1513 915 492 393 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3575
SphI G_CATG'C
Cuts at: 391 628 1128 391
Size: 237 500 2838
Fragments arranged by size:
2838 500 237
StyI C'CwwG_G
Cuts at: 107 180 709 1063 107
Size: 73 529 354 2619
Fragments arranged by size:
2619 529 354 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 233 316 1158 1291 1750 1959 233
Size: 83 842 133 459 209 1849
Fragments arranged by size:
1849 842 459 209 133 83
TatI w'GTAC_w
Cuts at: 3090 3090
Size: 3575
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3092 3124 3092
Size: 32 3543
Tth111I GACn'n_nGTC
Cuts at: 1372 1372
Size: 3575
XbaI T'CTAG_A
Cuts at: 606 606
Size: 3575
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1070 1070
Size: 3575
XmaI C'CCGG_G
Cuts at: 469 595 469
Size: 126 3449
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PmeI PpiI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BmgBI BmtI
Bpu10I BseRI BsgI BsiWI BsmBI BsrGI BssHII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI