Restriction map for
pF1KE0527
(Circular) (Six-base) MAPSORT of: pF1KE0527.seq Check: 6003 from: 1 to: 3845
pF1KE0527 3845 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 579 579
Size: 3845
AccI GT'mk_AC
Cuts at: 158 441 883 158
Size: 283 442 3120
Fragments arranged by size:
3120 442 283
Acc65I G'GTAC_C
Cuts at: 861 861
Size: 3845
AclI AA'CG_TT
Cuts at: 3685 3685
Size: 3845
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1670 2102 2849 3117 1670
Size: 432 747 268 2398
Fragments arranged by size:
2398 747 432 268
AflIII A'CryG_T
Cuts at: 1197 2301 3278 1197
Size: 1104 977 1764
Fragments arranged by size:
1764 1104 977
AgeI A'CCGG_T
Cuts at: 2233 2233
Size: 3845
AhdI GACnn_n'nnGTC
Cuts at: 135 135
Size: 3845
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 398 398
Size: 3845
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 538 570 538
Size: 32 3813
AlwNI CAG_nnn'CTG
Cuts at: 534 1150 2717 534
Size: 616 1567 1662
Fragments arranged by size:
1662 1567 616
ApaLI G'TGCA_C
Cuts at: 2615 2615
Size: 3845
ApoI r'AATT_y
Cuts at: 849 3198 3454 849
Size: 2349 256 1240
Fragments arranged by size:
2349 1240 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3845
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3845
AvaI C'yCGr_G
Cuts at: 490 865 490
Size: 375 3470
BamHI G'GATC_C
Cuts at: 833 870 833
Size: 37 3808
BanI G'GyrC_C
Cuts at: 430 486 861 1275 1523 1558 430
Size: 56 375 414 248 35 2717
Fragments arranged by size:
2717 414 375 248 56 35
BanII G_rGCy'C
Cuts at: 859 1889 859
Size: 1030 2815
BbeI G_GCGC'C
Cuts at: 1279 1527 1279
Size: 248 3597
BciVI GTATCCnnnnn_n'
Cuts at: 371 1743 2504 3845 371
Size: 1372 761 1341 371
Fragments arranged by size:
1372 1341 761 371
BclI T'GATC_A
Cuts at: 1365 1365
Size: 3845
BglI GCCn_nnn'nGGC
Cuts at: 808 808
Size: 3845
BglII A'GATC_T
Cuts at: 1360 3397 1360
Size: 2037 1808
BlpI GC'TnA_GC
Cuts at: 716 957 716
Size: 241 3604
Bme1580I G_kGCm'C
Cuts at: 387 491 1470 1563 2619 387
Size: 104 979 93 1056 1613
Fragments arranged by size:
1613 1056 979 104 93
BmrI ACTGGGnnnn_n'
Cuts at: 1057 1138 1473 3617 1057
Size: 81 335 2144 1285
Fragments arranged by size:
2144 1285 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2212 2212
Size: 3845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 637 565
Size: 72 3773
Bpu10I CC'TnA_GC
Cuts at: 328 328
Size: 3845
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 768 1021 1900 2392 2690 262
Size: 506 253 879 492 298 1417
Fragments arranged by size:
1417 879 506 492 298 253
BsaI GGTCTCn'nnnn_
Cuts at: 3549 3549
Size: 3845
BsaAI yAC'GTr
Cuts at: 1198 1198
Size: 3845
BsaBI GATnn'nnATC
Cuts at: 6 1359 3396 6
Size: 1353 2037 455
Fragments arranged by size:
2037 1353 455
BsaHI Gr'CG_yC
Cuts at: 346 1276 1524 2226 3722 346
Size: 930 248 702 1496 469
Fragments arranged by size:
1496 930 702 469 248
BsaWI w'CCGG_w
Cuts at: 1034 1555 2233 2507 2654 3065 1034
Size: 521 678 274 147 411 1814
Fragments arranged by size:
1814 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 538 568 538
Size: 30 3815
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 508 634 508
Size: 126 3719
BseYI C'CCAG_C
Cuts at: 140 145 1270 2028 2605 3101 140
Size: 5 1125 758 577 496 884
Fragments arranged by size:
1125 884 758 577 496 5
BsiEI CG_ry'CG
Cuts at: 104 1387 1433 2641 104
Size: 1283 46 1208 1308
Fragments arranged by size:
1308 1283 1208 46
BsiHKAI G_wGCw'C
Cuts at: 859 1637 1827 2619 859
Size: 778 190 792 2085
Fragments arranged by size:
2085 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 387 491 859 1470 1563 1637 1827 1889 2619
Size: 104 368 611 93 74 190 62 730
Cuts at: 2619 387
Size: 1613
Fragments arranged by size:
1613 730 611 368 190 104 93 74
62
BspEI T'CCGG_A
Cuts at: 3065 3065
Size: 3845
BspHI T'CATG_A
Cuts at: 393 3021 393
Size: 2628 1217
BspMI ACCTGCnnnn'nnnn_
Cuts at: 380 579 895 1109 1411 1792 380
Size: 199 316 214 302 381 2433
Fragments arranged by size:
2433 381 316 302 214 199
BsrBI CCG'CTC
Cuts at: 50 2137 2191 3675 3840 50
Size: 2087 54 1484 165 55
Fragments arranged by size:
2087 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 544 1757 544
Size: 1213 2632
BsrFI r'CCGG_y
Cuts at: 633 1843 2024 2233 633
Size: 1210 181 209 2245
Fragments arranged by size:
2245 1210 209 181
BssSI C'ACGA_G
Cuts at: 380 2116 2474 380
Size: 1736 358 1751
Fragments arranged by size:
1751 1736 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1222 1222
Size: 3845
BstBI TT'CG_AA
Cuts at: 822 2206 822
Size: 1384 2461
BstYI r'GATC_y
Cuts at: 1 833 870 1337 1360 1695 1941 2953 3039
Size: 832 37 467 23 335 246 1012 86
Cuts at: 3039 3051 3397 1
Size: 12 346 449
Fragments arranged by size:
1012 832 467 449 346 335 246 86
37 23 12
BstZ17I GTA'TAC
Cuts at: 442 442
Size: 3845
BtgI C'CryG_G
Cuts at: 107 203 426 521 107
Size: 96 223 95 3431
Fragments arranged by size:
3431 223 96 95
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1795 1976 2139 1795
Size: 181 163 3501
Fragments arranged by size:
3501 181 163
BtsI GCAGTG_nn'
Cuts at: 1088 1088
Size: 3845
DraI TTT'AAA
Cuts at: 844 844
Size: 3845
DrdI GACnn_nn'nnGTC
Cuts at: 1551 2409 1551
Size: 858 2987
EaeI y'GGCC_r
Cuts at: 311 582 667 767 1430 1604 1995 2022 3367
Size: 271 85 100 663 174 391 27 1345
Cuts at: 3367 311
Size: 789
Fragments arranged by size:
1345 789 663 391 271 174 100 85
27
EagI C'GGCC_G
Cuts at: 1430 1430
Size: 3845
EarI CTCTTCn'nnn_
Cuts at: 1868 2078 1868
Size: 210 3635
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2053 2363 2509 3655 2053
Size: 310 146 1146 2243
Fragments arranged by size:
2243 1146 310 146
EcoICRI GAG'CTC
Cuts at: 857 857
Size: 3845
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 637 1670 2102 2849 3117 565
Size: 72 1033 432 747 268 1293
Fragments arranged by size:
1293 1033 747 432 268 72
EcoNI CCTnn'n_nnAGG
Cuts at: 760 760
Size: 3845
EcoO109I rG'GnC_Cy
Cuts at: 531 569 984 531
Size: 38 415 3392
Fragments arranged by size:
3392 415 38
EcoRI G'AATT_C
Cuts at: 849 849
Size: 3845
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 258 1864 258
Size: 1606 2239
FspI TGC'GCA
Cuts at: 199 1626 199
Size: 1427 2418
HaeII r_GCGC'y
Cuts at: 1279 1527 2549 3528 1279
Size: 248 1022 979 1596
Fragments arranged by size:
1596 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 121 153 1687 1717 1719 1749 121
Size: 32 1534 30 2 30 2217
Fragments arranged by size:
2217 1534 32 30 30 2
HincII GTy'rAC
Cuts at: 884 884
Size: 3845
KasI G'GCGC_C
Cuts at: 1275 1523 1275
Size: 248 3597
KpnI G_GTAC'C
Cuts at: 865 865
Size: 3845
MluI A'CGCG_T
Cuts at: 3278 3278
Size: 3845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2516 2700 2516
Size: 184 3661
MscI TGG'CCA
Cuts at: 584 1606 584
Size: 1022 2823
MslI CAynn'nnrTG
Cuts at: 1834 1961 1834
Size: 127 3718
MspA1I CmG'CkG
Cuts at: 956 1270 1630 2643 2888 956
Size: 314 360 1013 245 1913
Fragments arranged by size:
1913 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2026 2026
Size: 3845
NarI GG'CG_CC
Cuts at: 1276 1524 1276
Size: 248 3597
NcoI C'CATG_G
Cuts at: 107 521 107
Size: 414 3431
NgoMIV G'CCGG_C
Cuts at: 2024 2024
Size: 3845
NspI r_CATG'y
Cuts at: 415 898 1398 2305 415
Size: 483 500 907 1955
Fragments arranged by size:
1955 907 500 483
PciI A'CATG_T
Cuts at: 2301 2301
Size: 3845
PflMI CCAn_nnn'nTGG
Cuts at: 462 475 462
Size: 13 3832
PfoI T'CCnGG_A
Cuts at: 755 755
Size: 3845
PmeI GTTT'AAAC
Cuts at: 844 844
Size: 3845
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 538 570 2984 3016 538
Size: 32 2414 32 1367
Fragments arranged by size:
2414 1367 32 32
PstI C_TGCA'G
Cuts at: 377 892 1577 377
Size: 515 685 2645
Fragments arranged by size:
2645 685 515
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3845
PvuII CAG'CTG
Cuts at: 1270 1630 1270
Size: 360 3485
RsrII CG'GwC_CG
Cuts at: 2040 2040
Size: 3845
SacI G_AGCT'C
Cuts at: 859 859
Size: 3845
SalI G'TCGA_C
Cuts at: 882 882
Size: 3845
SapI GCTCTTCn'nnn_
Cuts at: 1868 2078 1868
Size: 210 3635
SbfI CC_TGCA'GG
Cuts at: 377 892 377
Size: 515 3330
ScaI AGT'ACT
Cuts at: 3362 3362
Size: 3845
SexAI A'CCwGG_T
Cuts at: 131 131
Size: 3845
SfcI C'TryA_G
Cuts at: 33 373 888 1573 2566 2757 33
Size: 340 515 685 993 191 1121
Fragments arranged by size:
1121 993 685 515 340 191
SfoI GGC'GCC
Cuts at: 1277 1525 1277
Size: 248 3597
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3845
SmaI CCC'GGG
Cuts at: 867 867
Size: 3845
SmlI C'TyrA_G
Cuts at: 277 747 1000 1915 2407 2669 277
Size: 470 253 915 492 262 1453
Fragments arranged by size:
1453 915 492 470 262 253
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3845
SphI G_CATG'C
Cuts at: 415 898 1398 415
Size: 483 500 2862
Fragments arranged by size:
2862 500 483
StuI AGG'CCT
Cuts at: 802 802
Size: 3845
StyI C'CwwG_G
Cuts at: 107 521 979 1333 107
Size: 414 458 354 2619
Fragments arranged by size:
2619 458 414 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 316 700 1428 1561 2020 2229 316
Size: 384 728 133 459 209 1932
Fragments arranged by size:
1932 728 459 384 209 133
TatI w'GTAC_w
Cuts at: 3360 3360
Size: 3845
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3362 3394 3362
Size: 32 3813
Tth111I GACn'n_nGTC
Cuts at: 1642 1642
Size: 3845
XbaI T'CTAG_A
Cuts at: 876 876
Size: 3845
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1340 1340
Size: 3845
XmaI C'CCGG_G
Cuts at: 865 865
Size: 3845
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BstZ17I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI ApaI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BmgBI BmtI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI