Restriction map for
pF1KE0522
(Circular) (Six-base) MAPSORT of: pF1KE0522.seq Check: 6826 from: 1 to: 3593
pF1KE0522 3593 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 631 631
Size: 3593
Acc65I G'GTAC_C
Cuts at: 609 609
Size: 3593
AclI AA'CG_TT
Cuts at: 3433 3433
Size: 3593
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1418 1850 2597 2865 1418
Size: 432 747 268 2146
Fragments arranged by size:
2146 747 432 268
AflIII A'CryG_T
Cuts at: 945 2049 3026 945
Size: 1104 977 1512
Fragments arranged by size:
1512 1104 977
AgeI A'CCGG_T
Cuts at: 1981 1981
Size: 3593
AlwNI CAG_nnn'CTG
Cuts at: 376 898 2465 376
Size: 522 1567 1504
Fragments arranged by size:
1567 1504 522
ApaLI G'TGCA_C
Cuts at: 2363 2363
Size: 3593
ApoI r'AATT_y
Cuts at: 519 597 2946 3202 519
Size: 78 2349 256 910
Fragments arranged by size:
2349 910 256 78
AseI AT'TA_AT
Cuts at: 20 20
Size: 3593
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3593
AvaI C'yCGr_G
Cuts at: 613 613
Size: 3593
BamHI G'GATC_C
Cuts at: 618 618
Size: 3593
BanI G'GyrC_C
Cuts at: 609 1023 1271 1306 609
Size: 414 248 35 2896
Fragments arranged by size:
2896 414 248 35
BanII G_rGCy'C
Cuts at: 607 1637 607
Size: 1030 2563
BbeI G_GCGC'C
Cuts at: 1027 1275 1027
Size: 248 3345
BciVI GTATCCnnnnn_n'
Cuts at: 1491 2252 3593 1491
Size: 761 1341 1491
Fragments arranged by size:
1491 1341 761
BclI T'GATC_A
Cuts at: 1113 1113
Size: 3593
BglII A'GATC_T
Cuts at: 1108 3145 1108
Size: 2037 1556
BlpI GC'TnA_GC
Cuts at: 705 705
Size: 3593
Bme1580I G_kGCm'C
Cuts at: 1218 1311 2367 1218
Size: 93 1056 2444
Fragments arranged by size:
2444 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 805 886 1221 3365 805
Size: 81 335 2144 1033
Fragments arranged by size:
2144 1033 335 81
BmtI G_CTAG'C
Cuts at: 334 334
Size: 3593
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1960 1960
Size: 3593
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 339 339
Size: 3593
Bpu10I CC'TnA_GC
Cuts at: 287 287
Size: 3593
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 769 1648 2140 2438 325
Size: 444 879 492 298 1480
Fragments arranged by size:
1480 879 492 444 298
BsaI GGTCTCn'nnnn_
Cuts at: 3297 3297
Size: 3593
BsaAI yAC'GTr
Cuts at: 946 946
Size: 3593
BsaBI GATnn'nnATC
Cuts at: 6 1107 3144 6
Size: 1101 2037 455
Fragments arranged by size:
2037 1101 455
BsaHI Gr'CG_yC
Cuts at: 1024 1272 1974 3470 1024
Size: 248 702 1496 1147
Fragments arranged by size:
1496 1147 702 248
BsaWI w'CCGG_w
Cuts at: 782 1303 1981 2255 2402 2813 782
Size: 521 678 274 147 411 1562
Fragments arranged by size:
1562 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1018 1776 2353 2849 1018
Size: 758 577 496 1762
Fragments arranged by size:
1762 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1135 1181 2389 104
Size: 1031 46 1208 1308
Fragments arranged by size:
1308 1208 1031 46
BsiHKAI G_wGCw'C
Cuts at: 607 1385 1575 2367 607
Size: 778 190 792 1833
Fragments arranged by size:
1833 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 607 1218 1311 1385 1575 1637 2367 607
Size: 611 93 74 190 62 730 1833
Fragments arranged by size:
1833 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2813 2813
Size: 3593
BspHI T'CATG_A
Cuts at: 2769 2769
Size: 3593
BspMI ACCTGCnnnn'nnnn_
Cuts at: 384 643 857 1159 1540 384
Size: 259 214 302 381 2437
Fragments arranged by size:
2437 381 302 259 214
BsrBI CCG'CTC
Cuts at: 50 1885 1939 3423 3588 50
Size: 1835 54 1484 165 55
Fragments arranged by size:
1835 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 438 1505 438
Size: 1067 2526
BsrFI r'CCGG_y
Cuts at: 1591 1772 1981 1591
Size: 181 209 3203
Fragments arranged by size:
3203 209 181
BssSI C'ACGA_G
Cuts at: 1864 2222 1864
Size: 358 3235
BstAPI GCAn_nnn'nTGC
Cuts at: 376 970 376
Size: 594 2999
BstBI TT'CG_AA
Cuts at: 1954 1954
Size: 3593
BstYI r'GATC_y
Cuts at: 1 468 618 1085 1108 1443 1689 2701 2787
Size: 467 150 467 23 335 246 1012 86
Cuts at: 2787 2799 3145 1
Size: 12 346 449
Fragments arranged by size:
1012 467 467 449 346 335 246 150
86 23 12
BtgI C'CryG_G
Cuts at: 170 170
Size: 3593
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1543 1724 1887 1543
Size: 181 163 3249
Fragments arranged by size:
3249 181 163
BtsI GCAGTG_nn'
Cuts at: 836 836
Size: 3593
DraI TTT'AAA
Cuts at: 267 592 267
Size: 325 3268
DrdI GACnn_nn'nnGTC
Cuts at: 1299 2157 1299
Size: 858 2735
EaeI y'GGCC_r
Cuts at: 1178 1352 1743 1770 3115 1178
Size: 174 391 27 1345 1656
Fragments arranged by size:
1656 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1178 1178
Size: 3593
EarI CTCTTCn'nnn_
Cuts at: 1616 1826 1616
Size: 210 3383
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 307 1801 2111 2257 3403 307
Size: 1494 310 146 1146 497
Fragments arranged by size:
1494 1146 497 310 146
EcoICRI GAG'CTC
Cuts at: 605 605
Size: 3593
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 339 1418 1850 2597 2865 339
Size: 1079 432 747 268 1067
Fragments arranged by size:
1079 1067 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 373 732 373
Size: 359 3234
EcoRI G'AATT_C
Cuts at: 597 597
Size: 3593
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 319 1612 319
Size: 1293 2300
FspI TGC'GCA
Cuts at: 1374 1374
Size: 3593
HaeII r_GCGC'y
Cuts at: 1027 1275 2297 3276 1027
Size: 248 1022 979 1344
Fragments arranged by size:
1344 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1435 1465 1467 1497 1435
Size: 30 2 30 3531
Fragments arranged by size:
3531 30 30 2
HincII GTy'rAC
Cuts at: 632 632
Size: 3593
KasI G'GCGC_C
Cuts at: 1023 1271 1023
Size: 248 3345
KpnI G_GTAC'C
Cuts at: 613 613
Size: 3593
MluI A'CGCG_T
Cuts at: 3026 3026
Size: 3593
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2264 2448 2264
Size: 184 3409
MscI TGG'CCA
Cuts at: 1354 1354
Size: 3593
MslI CAynn'nnrTG
Cuts at: 1582 1709 1582
Size: 127 3466
MspA1I CmG'CkG
Cuts at: 704 1018 1378 2391 2636 704
Size: 314 360 1013 245 1661
Fragments arranged by size:
1661 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1774 1774
Size: 3593
NarI GG'CG_CC
Cuts at: 1024 1272 1024
Size: 248 3345
NcoI C'CATG_G
Cuts at: 170 170
Size: 3593
NgoMIV G'CCGG_C
Cuts at: 1772 1772
Size: 3593
NheI G'CTAG_C
Cuts at: 330 330
Size: 3593
NspI r_CATG'y
Cuts at: 646 1146 2053 646
Size: 500 907 2186
Fragments arranged by size:
2186 907 500
PciI A'CATG_T
Cuts at: 2049 2049
Size: 3593
PmeI GTTT'AAAC
Cuts at: 592 592
Size: 3593
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2732 2764 2732
Size: 32 3561
PpuMI rG'GwC_Cy
Cuts at: 373 373
Size: 3593
PstI C_TGCA'G
Cuts at: 329 640 1325 329
Size: 311 685 2597
Fragments arranged by size:
2597 685 311
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3593
PvuII CAG'CTG
Cuts at: 1018 1378 1018
Size: 360 3233
RsrII CG'GwC_CG
Cuts at: 1788 1788
Size: 3593
SacI G_AGCT'C
Cuts at: 607 607
Size: 3593
SalI G'TCGA_C
Cuts at: 630 630
Size: 3593
SapI GCTCTTCn'nnn_
Cuts at: 1616 1826 1616
Size: 210 3383
SbfI CC_TGCA'GG
Cuts at: 640 640
Size: 3593
ScaI AGT'ACT
Cuts at: 3110 3110
Size: 3593
SfcI C'TryA_G
Cuts at: 33 325 416 636 1321 2314 2505 33
Size: 292 91 220 685 993 191 1121
Fragments arranged by size:
1121 993 685 292 220 191 91
SfoI GGC'GCC
Cuts at: 1025 1273 1025
Size: 248 3345
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3593
SmaI CCC'GGG
Cuts at: 615 615
Size: 3593
SmlI C'TyrA_G
Cuts at: 304 748 1663 2155 2417 304
Size: 444 915 492 262 1480
Fragments arranged by size:
1480 915 492 444 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3593
SphI G_CATG'C
Cuts at: 646 1146 646
Size: 500 3093
StyI C'CwwG_G
Cuts at: 170 727 1081 170
Size: 557 354 2682
Fragments arranged by size:
2682 557 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1176 1309 1768 1977 1176
Size: 133 459 209 2792
Fragments arranged by size:
2792 459 209 133
TatI w'GTAC_w
Cuts at: 350 3108 350
Size: 2758 835
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3110 3142 3110
Size: 32 3561
Tth111I GACn'n_nGTC
Cuts at: 1390 1390
Size: 3593
XbaI T'CTAG_A
Cuts at: 624 624
Size: 3593
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1088 1088
Size: 3593
XmaI C'CCGG_G
Cuts at: 613 613
Size: 3593
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PciI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI