Restriction map for
pF1KE0521
(Circular) (Six-base) MAPSORT of: pF1KE0521.seq Check: 330 from: 1 to: 3941
pF1KE0521 3941 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AatII G_ACGT'C
Cuts at: 506 506
Size: 3941
AccI GT'mk_AC
Cuts at: 979 979
Size: 3941
Acc65I G'GTAC_C
Cuts at: 957 957
Size: 3941
AclI AA'CG_TT
Cuts at: 3781 3781
Size: 3941
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 1766 2198 2945 3213 445
Size: 1321 432 747 268 1173
Fragments arranged by size:
1321 1173 747 432 268
AflIII A'CryG_T
Cuts at: 155 304 1293 2397 3374 155
Size: 149 989 1104 977 722
Fragments arranged by size:
1104 989 977 722 149
AgeI A'CCGG_T
Cuts at: 2329 2329
Size: 3941
AhdI GACnn_n'nnGTC
Cuts at: 414 414
Size: 3941
AleI CACnn'nnGTG
Cuts at: 403 403
Size: 3941
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 598 598
Size: 3941
AlwNI CAG_nnn'CTG
Cuts at: 657 1246 2813 657
Size: 589 1567 1785
Fragments arranged by size:
1785 1567 589
ApaLI G'TGCA_C
Cuts at: 2711 2711
Size: 3941
ApoI r'AATT_y
Cuts at: 190 945 3294 3550 190
Size: 755 2349 256 581
Fragments arranged by size:
2349 755 581 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3941
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3941
AvaI C'yCGr_G
Cuts at: 362 961 362
Size: 599 3342
BamHI G'GATC_C
Cuts at: 966 966
Size: 3941
BanI G'GyrC_C
Cuts at: 559 811 957 1371 1619 1654 559
Size: 252 146 414 248 35 2846
Fragments arranged by size:
2846 414 252 248 146 35
BanII G_rGCy'C
Cuts at: 955 1985 955
Size: 1030 2911
BbeI G_GCGC'C
Cuts at: 1375 1623 1375
Size: 248 3693
BciVI GTATCCnnnnn_n'
Cuts at: 1839 2600 3941 1839
Size: 761 1341 1839
Fragments arranged by size:
1839 1341 761
BclI T'GATC_A
Cuts at: 1461 1461
Size: 3941
BglII A'GATC_T
Cuts at: 1456 3493 1456
Size: 2037 1904
BlpI GC'TnA_GC
Cuts at: 571 769 1053 571
Size: 198 284 3459
Fragments arranged by size:
3459 284 198
Bme1580I G_kGCm'C
Cuts at: 1566 1659 2715 1566
Size: 93 1056 2792
Fragments arranged by size:
2792 1056 93
BmgBI CAC'GTC
Cuts at: 405 405
Size: 3941
BmrI ACTGGGnnnn_n'
Cuts at: 909 1153 1234 1569 3713 909
Size: 244 81 335 2144 1137
Fragments arranged by size:
2144 1137 335 244 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2308 2308
Size: 3941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 618 652 607
Size: 11 34 3896
Fragments arranged by size:
3896 34 11
Bpu10I CC'TnA_GC
Cuts at: 484 484
Size: 3941
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1117 1996 2488 2786 1117
Size: 879 492 298 2272
Fragments arranged by size:
2272 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 438 898 3645 438
Size: 460 2747 734
Fragments arranged by size:
2747 734 460
BsaAI yAC'GTr
Cuts at: 307 756 855 1294 307
Size: 449 99 439 2954
Fragments arranged by size:
2954 449 439 99
BsaBI GATnn'nnATC
Cuts at: 6 1455 3492 6
Size: 1449 2037 455
Fragments arranged by size:
2037 1449 455
BsaHI Gr'CG_yC
Cuts at: 503 553 1372 1620 2322 3818 503
Size: 50 819 248 702 1496 626
Fragments arranged by size:
1496 819 702 626 248 50
BsaWI w'CCGG_w
Cuts at: 1130 1651 2329 2603 2750 3161 1130
Size: 521 678 274 147 411 1910
Fragments arranged by size:
1910 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 291 1366 2124 2701 3197 291
Size: 1075 758 577 496 1035
Fragments arranged by size:
1075 1035 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 778 778
Size: 3941
BsiEI CG_ry'CG
Cuts at: 104 1483 1529 2737 104
Size: 1379 46 1208 1308
Fragments arranged by size:
1379 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 490 674 698 955 1733 1923 2715 490
Size: 184 24 257 778 190 792 1716
Fragments arranged by size:
1716 792 778 257 190 184 24
BsmBI CGTCTCn'nnnn_
Cuts at: 494 494
Size: 3941
Bsp1286I G_dGCh'C
Cuts at: 490 674 698 955 1566 1659 1733 1923 1985
Size: 184 24 257 611 93 74 190 62
Cuts at: 1985 2715 490
Size: 730 1716
Fragments arranged by size:
1716 730 611 257 190 184 93 74
62 24
BspEI T'CCGG_A
Cuts at: 3161 3161
Size: 3941
BspHI T'CATG_A
Cuts at: 3117 3117
Size: 3941
BspMI ACCTGCnnnn'nnnn_
Cuts at: 344 991 1205 1507 1888 344
Size: 647 214 302 381 2397
Fragments arranged by size:
2397 647 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2233 2287 3771 3936 50
Size: 2183 54 1484 165 55
Fragments arranged by size:
2183 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1853 1853
Size: 3941
BsrFI r'CCGG_y
Cuts at: 1939 2120 2329 1939
Size: 181 209 3551
Fragments arranged by size:
3551 209 181
BsrGI T'GTAC_A
Cuts at: 452 452
Size: 3941
BssSI C'ACGA_G
Cuts at: 2212 2570 2212
Size: 358 3583
BstAPI GCAn_nnn'nTGC
Cuts at: 765 1318 765
Size: 553 3388
BstBI TT'CG_AA
Cuts at: 2302 2302
Size: 3941
BstXI CCAn_nnnn'nTGG
Cuts at: 405 687 405
Size: 282 3659
BstYI r'GATC_y
Cuts at: 1 242 966 1433 1456 1791 2037 3049 3135
Size: 241 724 467 23 335 246 1012 86
Cuts at: 3135 3147 3493 1
Size: 12 346 449
Fragments arranged by size:
1012 724 467 449 346 335 246 241
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 660 1891 2072 2235 660
Size: 1231 181 163 2366
Fragments arranged by size:
2366 1231 181 163
BtsI GCAGTG_nn'
Cuts at: 925 1184 925
Size: 259 3682
DraI TTT'AAA
Cuts at: 940 940
Size: 3941
DrdI GACnn_nn'nnGTC
Cuts at: 1647 2505 1647
Size: 858 3083
EaeI y'GGCC_r
Cuts at: 728 849 1526 1700 2091 2118 3463 728
Size: 121 677 174 391 27 1345 1206
Fragments arranged by size:
1345 1206 677 391 174 121 27
EagI C'GGCC_G
Cuts at: 1526 1526
Size: 3941
EarI CTCTTCn'nnn_
Cuts at: 631 701 1964 2174 631
Size: 70 1263 210 2398
Fragments arranged by size:
2398 1263 210 70
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2149 2459 2605 3751 2149
Size: 310 146 1146 2339
Fragments arranged by size:
2339 1146 310 146
EcoICRI GAG'CTC
Cuts at: 953 953
Size: 3941
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 607 618 652 1766 2198 2945 3213 445
Size: 162 11 34 1114 432 747 268 1173
Fragments arranged by size:
1173 1114 747 432 268 162 34 11
EcoNI CCTnn'n_nnAGG
Cuts at: 427 427
Size: 3941
EcoO109I rG'GnC_Cy
Cuts at: 888 1080 888
Size: 192 3749
EcoRI G'AATT_C
Cuts at: 190 945 190
Size: 755 3186
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 627 642 1960 627
Size: 15 1318 2608
Fragments arranged by size:
2608 1318 15
FspI TGC'GCA
Cuts at: 1722 1722
Size: 3941
HaeII r_GCGC'y
Cuts at: 658 1375 1623 2645 3624 658
Size: 717 248 1022 979 975
Fragments arranged by size:
1022 979 975 717 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 400 432 1783 1813 1815 1845 400
Size: 32 1351 30 2 30 2496
Fragments arranged by size:
2496 1351 32 30 30 2
HincII GTy'rAC
Cuts at: 980 980
Size: 3941
KasI G'GCGC_C
Cuts at: 1371 1619 1371
Size: 248 3693
KpnI G_GTAC'C
Cuts at: 961 961
Size: 3941
MluI A'CGCG_T
Cuts at: 155 3374 155
Size: 3219 722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2612 2796 2612
Size: 184 3757
MscI TGG'CCA
Cuts at: 1702 1702
Size: 3941
MslI CAynn'nnrTG
Cuts at: 403 1930 2057 403
Size: 1527 127 2287
Fragments arranged by size:
2287 1527 127
MspA1I CmG'CkG
Cuts at: 348 717 1052 1366 1726 2739 2984 348
Size: 369 335 314 360 1013 245 1305
Fragments arranged by size:
1305 1013 369 360 335 314 245
NaeI GCC'GGC
Cuts at: 2122 2122
Size: 3941
NarI GG'CG_CC
Cuts at: 1372 1620 1372
Size: 248 3693
NgoMIV G'CCGG_C
Cuts at: 2120 2120
Size: 3941
NspI r_CATG'y
Cuts at: 994 1494 2401 994
Size: 500 907 2534
Fragments arranged by size:
2534 907 500
PciI A'CATG_T
Cuts at: 2397 2397
Size: 3941
PfoI T'CCnGG_A
Cuts at: 629 686 629
Size: 57 3884
PmeI GTTT'AAAC
Cuts at: 940 940
Size: 3941
PmlI CAC'GTG
Cuts at: 307 855 307
Size: 548 3393
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3080 3112 3080
Size: 32 3909
PpuMI rG'GwC_Cy
Cuts at: 888 888
Size: 3941
PstI C_TGCA'G
Cuts at: 341 932 988 1673 341
Size: 591 56 685 2609
Fragments arranged by size:
2609 685 591 56
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3941
PvuII CAG'CTG
Cuts at: 348 717 1366 1726 348
Size: 369 649 360 2563
Fragments arranged by size:
2563 649 369 360
RsrII CG'GwC_CG
Cuts at: 2136 2136
Size: 3941
SacI G_AGCT'C
Cuts at: 955 955
Size: 3941
SalI G'TCGA_C
Cuts at: 978 978
Size: 3941
SapI GCTCTTCn'nnn_
Cuts at: 631 1964 2174 631
Size: 1333 210 2398
Fragments arranged by size:
2398 1333 210
SbfI CC_TGCA'GG
Cuts at: 988 988
Size: 3941
ScaI AGT'ACT
Cuts at: 3458 3458
Size: 3941
SfcI C'TryA_G
Cuts at: 33 195 337 928 984 1669 2662 2853 33
Size: 162 142 591 56 685 993 191 1121
Fragments arranged by size:
1121 993 685 591 191 162 142 56
SfoI GGC'GCC
Cuts at: 1373 1621 1373
Size: 248 3693
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3941
SmaI CCC'GGG
Cuts at: 963 963
Size: 3941
SmlI C'TyrA_G
Cuts at: 1096 2011 2503 2765 1096
Size: 915 492 262 2272
Fragments arranged by size:
2272 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3941
SphI G_CATG'C
Cuts at: 994 1494 994
Size: 500 3441
StyI C'CwwG_G
Cuts at: 662 1075 1429 662
Size: 413 354 3174
Fragments arranged by size:
3174 413 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 400 1524 1657 2116 2325 400
Size: 1124 133 459 209 2016
Fragments arranged by size:
2016 1124 459 209 133
TatI w'GTAC_w
Cuts at: 452 3456 452
Size: 3004 937
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3458 3490 3458
Size: 32 3909
Tth111I GACn'n_nGTC
Cuts at: 1738 1738
Size: 3941
XbaI T'CTAG_A
Cuts at: 972 972
Size: 3941
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 454 1436 454
Size: 982 2959
XmaI C'CCGG_G
Cuts at: 961 961
Size: 3941
ZraI GAC'GTC
Cuts at: 504 504
Size: 3941
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PfoI PmeI PmlI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AloI ApaI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BglI BmtI BsaXI BseRI
BsiWI BsmI BssHII BstEII BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI