Restriction map for
pF1KE0516
(Circular) (Six-base) MAPSORT of: pF1KE0516.seq Check: 4969 from: 1 to: 4034
pF1KE0516 4034 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 1072 1072
Size: 4034
Acc65I G'GTAC_C
Cuts at: 1050 1050
Size: 4034
AclI AA'CG_TT
Cuts at: 3874 3874
Size: 4034
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1859 2291 3038 3306 1859
Size: 432 747 268 2587
Fragments arranged by size:
2587 747 432 268
AflIII A'CryG_T
Cuts at: 1386 2490 3467 1386
Size: 1104 977 1953
Fragments arranged by size:
1953 1104 977
AgeI A'CCGG_T
Cuts at: 2422 2422
Size: 4034
AlwNI CAG_nnn'CTG
Cuts at: 1339 2906 1339
Size: 1567 2467
ApaLI G'TGCA_C
Cuts at: 2804 2804
Size: 4034
ApoI r'AATT_y
Cuts at: 475 720 939 1038 3387 3643 475
Size: 245 219 99 2349 256 866
Fragments arranged by size:
2349 866 256 245 219 99
AseI AT'TA_AT
Cuts at: 20 20
Size: 4034
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4034
AvaI C'yCGr_G
Cuts at: 340 1054 340
Size: 714 3320
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 390 423 390
Size: 33 4001
BamHI G'GATC_C
Cuts at: 1059 1059
Size: 4034
BanI G'GyrC_C
Cuts at: 1050 1464 1712 1747 1050
Size: 414 248 35 3337
Fragments arranged by size:
3337 414 248 35
BanII G_rGCy'C
Cuts at: 277 1048 2078 277
Size: 771 1030 2233
Fragments arranged by size:
2233 1030 771
BbeI G_GCGC'C
Cuts at: 1468 1716 1468
Size: 248 3786
BbsI GAAGACnn'nnnn_
Cuts at: 1002 1002
Size: 4034
BciVI GTATCCnnnnn_n'
Cuts at: 1932 2693 4034 1932
Size: 761 1341 1932
Fragments arranged by size:
1932 1341 761
BclI T'GATC_A
Cuts at: 1554 1554
Size: 4034
BglII A'GATC_T
Cuts at: 1549 3586 1549
Size: 2037 1997
BlpI GC'TnA_GC
Cuts at: 119 1146 119
Size: 1027 3007
Bme1580I G_kGCm'C
Cuts at: 1659 1752 2808 1659
Size: 93 1056 2885
Fragments arranged by size:
2885 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1246 1327 1662 3806 1246
Size: 81 335 2144 1474
Fragments arranged by size:
2144 1474 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2401 2401
Size: 4034
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 957 957
Size: 4034
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 1210 2089 2581 2879 640
Size: 570 879 492 298 1795
Fragments arranged by size:
1795 879 570 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3738 3738
Size: 4034
BsaAI yAC'GTr
Cuts at: 406 1387 406
Size: 981 3053
BsaBI GATnn'nnATC
Cuts at: 6 1548 3585 6
Size: 1542 2037 455
Fragments arranged by size:
2037 1542 455
BsaHI Gr'CG_yC
Cuts at: 1465 1713 2415 3911 1465
Size: 248 702 1496 1588
Fragments arranged by size:
1588 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1223 1744 2422 2696 2843 3254 1223
Size: 521 678 274 147 411 2003
Fragments arranged by size:
2003 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1459 2217 2794 3290 1459
Size: 758 577 496 2203
Fragments arranged by size:
2203 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 969 969
Size: 4034
BsiEI CG_ry'CG
Cuts at: 104 1576 1622 2830 104
Size: 1472 46 1208 1308
Fragments arranged by size:
1472 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 277 973 1048 1826 2016 2808 277
Size: 696 75 778 190 792 1503
Fragments arranged by size:
1503 792 778 696 190 75
BsmI GAATG_Cn'
Cuts at: 919 919
Size: 4034
Bsp1286I G_dGCh'C
Cuts at: 277 973 1048 1659 1752 1826 2016 2078 2808
Size: 696 75 611 93 74 190 62 730
Cuts at: 2808 277
Size: 1503
Fragments arranged by size:
1503 730 696 611 190 93 75 74
62
BspEI T'CCGG_A
Cuts at: 3254 3254
Size: 4034
BspHI T'CATG_A
Cuts at: 809 3210 809
Size: 2401 1633
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1084 1298 1600 1981 1084
Size: 214 302 381 3137
Fragments arranged by size:
3137 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2326 2380 3864 4029 50
Size: 2276 54 1484 165 55
Fragments arranged by size:
2276 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 924 1946 924
Size: 1022 3012
BsrFI r'CCGG_y
Cuts at: 2032 2213 2422 2032
Size: 181 209 3644
Fragments arranged by size:
3644 209 181
BssSI C'ACGA_G
Cuts at: 2305 2663 2305
Size: 358 3676
BstAPI GCAn_nnn'nTGC
Cuts at: 1411 1411
Size: 4034
BstBI TT'CG_AA
Cuts at: 2395 2395
Size: 4034
BstYI r'GATC_y
Cuts at: 1 432 442 1059 1526 1549 1884 2130 3142
Size: 431 10 617 467 23 335 246 1012
Cuts at: 3142 3228 3240 3586 1
Size: 86 12 346 449
Fragments arranged by size:
1012 617 467 449 431 346 335 246
86 23 12 10
Bsu36I CC'TnA_GG
Cuts at: 253 253
Size: 4034
BtgI C'CryG_G
Cuts at: 107 437 107
Size: 330 3704
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1984 2165 2328 1984
Size: 181 163 3690
Fragments arranged by size:
3690 181 163
BtsI GCAGTG_nn'
Cuts at: 113 129 346 680 1277 113
Size: 16 217 334 597 2870
Fragments arranged by size:
2870 597 334 217 16
DraI TTT'AAA
Cuts at: 311 708 1033 311
Size: 397 325 3312
Fragments arranged by size:
3312 397 325
DrdI GACnn_nn'nnGTC
Cuts at: 1740 2598 1740
Size: 858 3176
EaeI y'GGCC_r
Cuts at: 110 1619 1793 2184 2211 3556 110
Size: 1509 174 391 27 1345 588
Fragments arranged by size:
1509 1345 588 391 174 27
EagI C'GGCC_G
Cuts at: 1619 1619
Size: 4034
EarI CTCTTCn'nnn_
Cuts at: 976 2057 2267 976
Size: 1081 210 2743
Fragments arranged by size:
2743 1081 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2242 2552 2698 3844 2242
Size: 310 146 1146 2432
Fragments arranged by size:
2432 1146 310 146
EcoICRI GAG'CTC
Cuts at: 275 1046 275
Size: 771 3263
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 957 1859 2291 3038 3306 957
Size: 902 432 747 268 1685
Fragments arranged by size:
1685 902 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 258 615 1173 258
Size: 357 558 3119
Fragments arranged by size:
3119 558 357
EcoRI G'AATT_C
Cuts at: 1038 1038
Size: 4034
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2053 2053
Size: 4034
FspI TGC'GCA
Cuts at: 1815 1815
Size: 4034
HaeII r_GCGC'y
Cuts at: 1468 1716 2738 3717 1468
Size: 248 1022 979 1785
Fragments arranged by size:
1785 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 181 213 867 899 1876 1906 1908 1938 181
Size: 32 654 32 977 30 2 30 2277
Fragments arranged by size:
2277 977 654 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1073 1073
Size: 4034
HindIII A'AGCT_T
Cuts at: 534 534
Size: 4034
KasI G'GCGC_C
Cuts at: 1464 1712 1464
Size: 248 3786
KpnI G_GTAC'C
Cuts at: 1054 1054
Size: 4034
MluI A'CGCG_T
Cuts at: 3467 3467
Size: 4034
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2705 2889 2705
Size: 184 3850
MscI TGG'CCA
Cuts at: 112 1795 112
Size: 1683 2351
MslI CAynn'nnrTG
Cuts at: 296 2023 2150 296
Size: 1727 127 2180
Fragments arranged by size:
2180 1727 127
MspA1I CmG'CkG
Cuts at: 1145 1459 1819 2832 3077 1145
Size: 314 360 1013 245 2102
Fragments arranged by size:
2102 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2215 2215
Size: 4034
NarI GG'CG_CC
Cuts at: 1465 1713 1465
Size: 248 3786
NcoI C'CATG_G
Cuts at: 107 437 107
Size: 330 3704
NgoMIV G'CCGG_C
Cuts at: 2213 2213
Size: 4034
NspI r_CATG'y
Cuts at: 1087 1587 2494 1087
Size: 500 907 2627
Fragments arranged by size:
2627 907 500
PciI A'CATG_T
Cuts at: 2490 2490
Size: 4034
PfoI T'CCnGG_A
Cuts at: 973 973
Size: 4034
PmeI GTTT'AAAC
Cuts at: 1033 1033
Size: 4034
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3173 3205 3173
Size: 32 4002
PpuMI rG'GwC_Cy
Cuts at: 615 615
Size: 4034
PstI C_TGCA'G
Cuts at: 1081 1766 1081
Size: 685 3349
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4034
PvuII CAG'CTG
Cuts at: 1459 1819 1459
Size: 360 3674
RsrII CG'GwC_CG
Cuts at: 2229 2229
Size: 4034
SacI G_AGCT'C
Cuts at: 277 1048 277
Size: 771 3263
SalI G'TCGA_C
Cuts at: 1071 1071
Size: 4034
SapI GCTCTTCn'nnn_
Cuts at: 2057 2267 2057
Size: 210 3824
SbfI CC_TGCA'GG
Cuts at: 1081 1081
Size: 4034
ScaI AGT'ACT
Cuts at: 416 3551 416
Size: 3135 899
SfcI C'TryA_G
Cuts at: 33 1077 1762 2755 2946 33
Size: 1044 685 993 191 1121
Fragments arranged by size:
1121 1044 993 685 191
SfoI GGC'GCC
Cuts at: 1466 1714 1466
Size: 248 3786
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4034
SmaI CCC'GGG
Cuts at: 342 1056 342
Size: 714 3320
SmlI C'TyrA_G
Cuts at: 619 1189 2104 2596 2858 619
Size: 570 915 492 262 1795
Fragments arranged by size:
1795 915 570 492 262
SnaBI TAC'GTA
Cuts at: 406 406
Size: 4034
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4034
SphI G_CATG'C
Cuts at: 1087 1587 1087
Size: 500 3534
StyI C'CwwG_G
Cuts at: 107 437 1168 1522 107
Size: 330 731 354 2619
Fragments arranged by size:
2619 731 354 330
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1617 1750 2209 2418 1617
Size: 133 459 209 3233
Fragments arranged by size:
3233 459 209 133
TatI w'GTAC_w
Cuts at: 414 529 642 3549 414
Size: 115 113 2907 899
Fragments arranged by size:
2907 899 115 113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 978 1010 3551 3583 978
Size: 32 2541 32 1429
Fragments arranged by size:
2541 1429 32 32
Tth111I GACn'n_nGTC
Cuts at: 1831 1831
Size: 4034
XbaI T'CTAG_A
Cuts at: 891 1065 891
Size: 174 3860
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1529 1529
Size: 4034
XmaI C'CCGG_G
Cuts at: 340 1054 340
Size: 714 3320
XmnI GAAnn'nnTTC
Cuts at: 1009 1009
Size: 4034
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI
SspI StuI SwaI XhoI ZraI