Restriction map for
pF1KE0515
(Circular) (Six-base) MAPSORT of: pF1KE0515.seq Check: 6443 from: 1 to: 3764
pF1KE0515 3764 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 802 802
Size: 3764
Acc65I G'GTAC_C
Cuts at: 780 780
Size: 3764
AclI AA'CG_TT
Cuts at: 3604 3604
Size: 3764
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1589 2021 2768 3036 1589
Size: 432 747 268 2317
Fragments arranged by size:
2317 747 432 268
AflIII A'CryG_T
Cuts at: 401 1116 2220 3197 401
Size: 715 1104 977 968
Fragments arranged by size:
1104 977 968 715
AgeI A'CCGG_T
Cuts at: 2152 2152
Size: 3764
AlwNI CAG_nnn'CTG
Cuts at: 565 1069 2636 565
Size: 504 1567 1693
Fragments arranged by size:
1693 1567 504
ApaLI G'TGCA_C
Cuts at: 2534 2534
Size: 3764
ApoI r'AATT_y
Cuts at: 616 768 3117 3373 616
Size: 152 2349 256 1007
Fragments arranged by size:
2349 1007 256 152
AseI AT'TA_AT
Cuts at: 20 20
Size: 3764
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
AvaI C'yCGr_G
Cuts at: 186 784 186
Size: 598 3166
BamHI G'GATC_C
Cuts at: 789 789
Size: 3764
BanI G'GyrC_C
Cuts at: 158 780 1194 1442 1477 158
Size: 622 414 248 35 2445
Fragments arranged by size:
2445 622 414 248 35
BanII G_rGCy'C
Cuts at: 778 1808 778
Size: 1030 2734
BbeI G_GCGC'C
Cuts at: 162 1198 1446 162
Size: 1036 248 2480
Fragments arranged by size:
2480 1036 248
BbsI GAAGACnn'nnnn_
Cuts at: 713 713
Size: 3764
BciVI GTATCCnnnnn_n'
Cuts at: 1662 2423 3764 1662
Size: 761 1341 1662
Fragments arranged by size:
1662 1341 761
BclI T'GATC_A
Cuts at: 1284 1284
Size: 3764
BglI GCCn_nnn'nGGC
Cuts at: 418 418
Size: 3764
BglII A'GATC_T
Cuts at: 1279 3316 1279
Size: 2037 1727
BlpI GC'TnA_GC
Cuts at: 876 876
Size: 3764
Bme1580I G_kGCm'C
Cuts at: 1389 1482 2538 1389
Size: 93 1056 2615
Fragments arranged by size:
2615 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 158 976 1057 1392 3536 158
Size: 818 81 335 2144 386
Fragments arranged by size:
2144 818 386 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 3764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 542 542
Size: 3764
Bpu10I CC'TnA_GC
Cuts at: 566 566
Size: 3764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 431 940 1819 2311 2609 431
Size: 509 879 492 298 1586
Fragments arranged by size:
1586 879 509 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3468 3468
Size: 3764
BsaAI yAC'GTr
Cuts at: 1117 1117
Size: 3764
BsaBI GATnn'nnATC
Cuts at: 6 1278 3315 6
Size: 1272 2037 455
Fragments arranged by size:
2037 1272 455
BsaHI Gr'CG_yC
Cuts at: 159 1195 1443 2145 3641 159
Size: 1036 248 702 1496 282
Fragments arranged by size:
1496 1036 702 282 248
BsaWI w'CCGG_w
Cuts at: 953 1474 2152 2426 2573 2984 953
Size: 521 678 274 147 411 1733
Fragments arranged by size:
1733 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 467 497 467
Size: 30 3734
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 290 290
Size: 3764
BseYI C'CCAG_C
Cuts at: 1189 1947 2524 3020 1189
Size: 758 577 496 1933
Fragments arranged by size:
1933 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 527 318
Size: 209 3555
BsiEI CG_ry'CG
Cuts at: 104 1306 1352 2560 104
Size: 1202 46 1208 1308
Fragments arranged by size:
1308 1208 1202 46
BsiHKAI G_wGCw'C
Cuts at: 778 1556 1746 2538 778
Size: 778 190 792 2004
Fragments arranged by size:
2004 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 778 1389 1482 1556 1746 1808 2538 778
Size: 611 93 74 190 62 730 2004
Fragments arranged by size:
2004 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2984 2984
Size: 3764
BspHI T'CATG_A
Cuts at: 266 2940 266
Size: 2674 1090
BspMI ACCTGCnnnn'nnnn_
Cuts at: 202 365 814 1028 1330 1711 202
Size: 163 449 214 302 381 2255
Fragments arranged by size:
2255 449 381 302 214 163
BsrBI CCG'CTC
Cuts at: 50 2056 2110 3594 3759 50
Size: 2006 54 1484 165 55
Fragments arranged by size:
2006 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1676 1676
Size: 3764
BsrFI r'CCGG_y
Cuts at: 1762 1943 2152 1762
Size: 181 209 3374
Fragments arranged by size:
3374 209 181
BssSI C'ACGA_G
Cuts at: 2035 2393 2035
Size: 358 3406
BstAPI GCAn_nnn'nTGC
Cuts at: 1141 1141
Size: 3764
BstBI TT'CG_AA
Cuts at: 2125 2125
Size: 3764
BstXI CCAn_nnnn'nTGG
Cuts at: 462 462
Size: 3764
BstYI r'GATC_y
Cuts at: 1 789 1256 1279 1614 1860 2872 2958 2970
Size: 788 467 23 335 246 1012 86 12
Cuts at: 2970 3316 1
Size: 346 449
Fragments arranged by size:
1012 788 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3764
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1714 1895 2058 1714
Size: 181 163 3420
Fragments arranged by size:
3420 181 163
BtsI GCAGTG_nn'
Cuts at: 1007 1007
Size: 3764
DraI TTT'AAA
Cuts at: 763 763
Size: 3764
DrdI GACnn_nn'nnGTC
Cuts at: 1470 2328 1470
Size: 858 2906
EaeI y'GGCC_r
Cuts at: 1349 1523 1914 1941 3286 1349
Size: 174 391 27 1345 1827
Fragments arranged by size:
1827 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1349 1349
Size: 3764
EarI CTCTTCn'nnn_
Cuts at: 578 1787 1997 578
Size: 1209 210 2345
Fragments arranged by size:
2345 1209 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1972 2282 2428 3574 1972
Size: 310 146 1146 2162
Fragments arranged by size:
2162 1146 310 146
EcoICRI GAG'CTC
Cuts at: 776 776
Size: 3764
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 542 1589 2021 2768 3036 542
Size: 1047 432 747 268 1270
Fragments arranged by size:
1270 1047 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 643 643
Size: 3764
EcoO109I rG'GnC_Cy
Cuts at: 191 419 534 636 903 191
Size: 228 115 102 267 3052
Fragments arranged by size:
3052 267 228 115 102
EcoRI G'AATT_C
Cuts at: 616 768 616
Size: 152 3612
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1783 1783
Size: 3764
FspI TGC'GCA
Cuts at: 1545 1545
Size: 3764
HaeII r_GCGC'y
Cuts at: 162 252 1198 1446 2468 3447 162
Size: 90 946 248 1022 979 479
Fragments arranged by size:
1022 979 946 479 248 90
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 653 685 1606 1636 1638 1668 653
Size: 32 921 30 2 30 2749
Fragments arranged by size:
2749 921 32 30 30 2
HincII GTy'rAC
Cuts at: 803 803
Size: 3764
KasI G'GCGC_C
Cuts at: 158 1194 1442 158
Size: 1036 248 2480
Fragments arranged by size:
2480 1036 248
KpnI G_GTAC'C
Cuts at: 784 784
Size: 3764
MluI A'CGCG_T
Cuts at: 3197 3197
Size: 3764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2435 2619 2435
Size: 184 3580
MscI TGG'CCA
Cuts at: 1525 1525
Size: 3764
MslI CAynn'nnrTG
Cuts at: 1753 1880 1753
Size: 127 3637
MspA1I CmG'CkG
Cuts at: 875 1189 1549 2562 2807 875
Size: 314 360 1013 245 1832
Fragments arranged by size:
1832 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1945 1945
Size: 3764
NarI GG'CG_CC
Cuts at: 159 1195 1443 159
Size: 1036 248 2480
Fragments arranged by size:
2480 1036 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3764
NgoMIV G'CCGG_C
Cuts at: 1943 1943
Size: 3764
NspI r_CATG'y
Cuts at: 405 817 1317 2224 405
Size: 412 500 907 1945
Fragments arranged by size:
1945 907 500 412
PasI CC'CwG_GG
Cuts at: 639 639
Size: 3764
PciI A'CATG_T
Cuts at: 401 2220 401
Size: 1819 1945
PflMI CCAn_nnn'nTGG
Cuts at: 258 258
Size: 3764
PfoI T'CCnGG_A
Cuts at: 602 695 602
Size: 93 3671
PmeI GTTT'AAAC
Cuts at: 763 763
Size: 3764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2903 2935 2903
Size: 32 3732
PpuMI rG'GwC_Cy
Cuts at: 191 191
Size: 3764
PstI C_TGCA'G
Cuts at: 811 1496 811
Size: 685 3079
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3764
PvuII CAG'CTG
Cuts at: 1189 1549 1189
Size: 360 3404
RsrII CG'GwC_CG
Cuts at: 1959 1959
Size: 3764
SacI G_AGCT'C
Cuts at: 778 778
Size: 3764
SalI G'TCGA_C
Cuts at: 801 801
Size: 3764
SapI GCTCTTCn'nnn_
Cuts at: 578 1787 1997 578
Size: 1209 210 2345
Fragments arranged by size:
2345 1209 210
SbfI CC_TGCA'GG
Cuts at: 811 811
Size: 3764
ScaI AGT'ACT
Cuts at: 3281 3281
Size: 3764
SfcI C'TryA_G
Cuts at: 33 292 423 807 1492 2485 2676 33
Size: 259 131 384 685 993 191 1121
Fragments arranged by size:
1121 993 685 384 259 191 131
SfoI GGC'GCC
Cuts at: 160 1196 1444 160
Size: 1036 248 2480
Fragments arranged by size:
2480 1036 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
SmaI CCC'GGG
Cuts at: 188 786 188
Size: 598 3166
SmlI C'TyrA_G
Cuts at: 446 919 1834 2326 2588 446
Size: 473 915 492 262 1622
Fragments arranged by size:
1622 915 492 473 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3764
SphI G_CATG'C
Cuts at: 817 1317 817
Size: 500 3264
StyI C'CwwG_G
Cuts at: 107 898 1252 107
Size: 791 354 2619
Fragments arranged by size:
2619 791 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 590 1347 1480 1939 2148 590
Size: 757 133 459 209 2206
Fragments arranged by size:
2206 757 459 209 133
TatI w'GTAC_w
Cuts at: 3279 3279
Size: 3764
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3281 3313 3281
Size: 32 3732
Tth111I GACn'n_nGTC
Cuts at: 1561 1561
Size: 3764
XbaI T'CTAG_A
Cuts at: 795 795
Size: 3764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 459 1259 459
Size: 800 2964
XmaI C'CCGG_G
Cuts at: 186 784 186
Size: 598 3166
XmnI GAAnn'nnTTC
Cuts at: 616 616
Size: 3764
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI