Restriction map for
pF1KE0514
(Circular) (Six-base) MAPSORT of: pF1KE0514.seq Check: 3194 from: 1 to: 3800
pF1KE0514 3800 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 838 838
Size: 3800
Acc65I G'GTAC_C
Cuts at: 816 816
Size: 3800
AclI AA'CG_TT
Cuts at: 3640 3640
Size: 3800
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 430 1625 2057 2804 3072 277
Size: 153 1195 432 747 268 1005
Fragments arranged by size:
1195 1005 747 432 268 153
AflIII A'CryG_T
Cuts at: 1152 2256 3233 1152
Size: 1104 977 1719
Fragments arranged by size:
1719 1104 977
AgeI A'CCGG_T
Cuts at: 2188 2188
Size: 3800
AlwNI CAG_nnn'CTG
Cuts at: 545 612 1105 2672 545
Size: 67 493 1567 1673
Fragments arranged by size:
1673 1567 493 67
ApaI G_GGCC'C
Cuts at: 389 578 389
Size: 189 3611
ApaLI G'TGCA_C
Cuts at: 484 2570 484
Size: 2086 1714
ApoI r'AATT_y
Cuts at: 804 3153 3409 804
Size: 2349 256 1195
Fragments arranged by size:
2349 1195 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3800
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3800
AvaI C'yCGr_G
Cuts at: 474 820 474
Size: 346 3454
BamHI G'GATC_C
Cuts at: 825 825
Size: 3800
BanI G'GyrC_C
Cuts at: 172 816 1230 1478 1513 172
Size: 644 414 248 35 2459
Fragments arranged by size:
2459 644 414 248 35
BanII G_rGCy'C
Cuts at: 326 389 578 814 1844 326
Size: 63 189 236 1030 2282
Fragments arranged by size:
2282 1030 236 189 63
BbeI G_GCGC'C
Cuts at: 1234 1482 1234
Size: 248 3552
BbsI GAAGACnn'nnnn_
Cuts at: 555 555
Size: 3800
BbvCI CC'TCA_GC
Cuts at: 592 592
Size: 3800
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 465 499 465
Size: 34 3766
BciVI GTATCCnnnnn_n'
Cuts at: 1698 2459 3800 1698
Size: 761 1341 1698
Fragments arranged by size:
1698 1341 761
BclI T'GATC_A
Cuts at: 1320 1320
Size: 3800
BglII A'GATC_T
Cuts at: 206 1315 3352 206
Size: 1109 2037 654
Fragments arranged by size:
2037 1109 654
BlpI GC'TnA_GC
Cuts at: 912 912
Size: 3800
Bme1580I G_kGCm'C
Cuts at: 389 488 578 1425 1518 2574 389
Size: 99 90 847 93 1056 1615
Fragments arranged by size:
1615 1056 847 99 93 90
BmrI ACTGGGnnnn_n'
Cuts at: 777 1012 1093 1428 3572 777
Size: 235 81 335 2144 1005
Fragments arranged by size:
2144 1005 335 235 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 648 2167 648
Size: 1519 2281
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 358 759 223
Size: 135 401 3264
Fragments arranged by size:
3264 401 135
Bpu10I CC'TnA_GC
Cuts at: 592 592
Size: 3800
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 301 976 1855 2347 2645 198
Size: 103 675 879 492 298 1353
Fragments arranged by size:
1353 879 675 492 298 103
BsaI GGTCTCn'nnnn_
Cuts at: 3504 3504
Size: 3800
BsaAI yAC'GTr
Cuts at: 429 1153 429
Size: 724 3076
BsaBI GATnn'nnATC
Cuts at: 6 1314 3351 6
Size: 1308 2037 455
Fragments arranged by size:
2037 1308 455
BsaHI Gr'CG_yC
Cuts at: 560 1231 1479 2181 3677 560
Size: 671 248 702 1496 683
Fragments arranged by size:
1496 702 683 671 248
BsaWI w'CCGG_w
Cuts at: 989 1510 2188 2462 2609 3020 989
Size: 521 678 274 147 411 1769
Fragments arranged by size:
1769 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 232 262 490 520 232
Size: 30 228 30 3512
Fragments arranged by size:
3512 228 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 511 751 511
Size: 240 3560
BseYI C'CCAG_C
Cuts at: 530 612 679 1225 1983 2560 3056 530
Size: 82 67 546 758 577 496 1274
Fragments arranged by size:
1274 758 577 546 496 82 67
BsiEI CG_ry'CG
Cuts at: 104 663 1342 1388 2596 104
Size: 559 679 46 1208 1308
Fragments arranged by size:
1308 1208 679 559 46
BsiHKAI G_wGCw'C
Cuts at: 359 377 488 814 1592 1782 2574 359
Size: 18 111 326 778 190 792 1585
Fragments arranged by size:
1585 792 778 326 190 111 18
Bsp1286I G_dGCh'C
Cuts at: 326 359 377 389 488 578 814 1425 1518
Size: 33 18 12 99 90 236 611 93
Cuts at: 1518 1592 1782 1844 2574 326
Size: 74 190 62 730 1552
Fragments arranged by size:
1552 730 611 236 190 99 93 90
74 62 33 18 12
BspEI T'CCGG_A
Cuts at: 3020 3020
Size: 3800
BspHI T'CATG_A
Cuts at: 2976 2976
Size: 3800
BspMI ACCTGCnnnn'nnnn_
Cuts at: 850 1064 1366 1747 850
Size: 214 302 381 2903
Fragments arranged by size:
2903 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2092 2146 3630 3795 50
Size: 2042 54 1484 165 55
Fragments arranged by size:
2042 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1712 1712
Size: 3800
BsrFI r'CCGG_y
Cuts at: 1798 1979 2188 1798
Size: 181 209 3410
Fragments arranged by size:
3410 209 181
BssSI C'ACGA_G
Cuts at: 2071 2429 2071
Size: 358 3442
BstAPI GCAn_nnn'nTGC
Cuts at: 1177 1177
Size: 3800
BstBI TT'CG_AA
Cuts at: 2161 2161
Size: 3800
BstYI r'GATC_y
Cuts at: 1 206 299 752 770 778 825 1292 1315
Size: 205 93 453 18 8 47 467 23
Cuts at: 1315 1650 1896 2908 2994 3006 3352 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 453 449 346 335 246 205
93 86 47 23 18 12 8
BtgI C'CryG_G
Cuts at: 107 107
Size: 3800
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 470 1750 1931 2094 470
Size: 1280 181 163 2176
Fragments arranged by size:
2176 1280 181 163
BtsI GCAGTG_nn'
Cuts at: 1043 1043
Size: 3800
DraI TTT'AAA
Cuts at: 654 799 654
Size: 145 3655
DraIII CAC_nnn'GTG
Cuts at: 432 432
Size: 3800
DrdI GACnn_nn'nnGTC
Cuts at: 1506 2364 1506
Size: 858 2942
EaeI y'GGCC_r
Cuts at: 1385 1559 1950 1977 3322 1385
Size: 174 391 27 1345 1863
Fragments arranged by size:
1863 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1385 1385
Size: 3800
EarI CTCTTCn'nnn_
Cuts at: 710 1823 2033 710
Size: 1113 210 2477
Fragments arranged by size:
2477 1113 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2008 2318 2464 3610 2008
Size: 310 146 1146 2198
Fragments arranged by size:
2198 1146 310 146
EcoICRI GAG'CTC
Cuts at: 812 812
Size: 3800
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 277 358 430 759 1625 2057 2804 3072
Size: 54 81 72 329 866 432 747 268
Cuts at: 3072 223
Size: 951
Fragments arranged by size:
951 866 747 432 329 268 81 72
54
EcoO109I rG'GnC_Cy
Cuts at: 182 386 574 575 702 939 182
Size: 204 188 1 127 237 3043
Fragments arranged by size:
3043 237 204 188 127 1
EcoRI G'AATT_C
Cuts at: 804 804
Size: 3800
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 342 1819 342
Size: 1477 2323
FspI TGC'GCA
Cuts at: 1581 1581
Size: 3800
HaeII r_GCGC'y
Cuts at: 1234 1482 2504 3483 1234
Size: 248 1022 979 1551
Fragments arranged by size:
1551 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 406 438 490 522 1642 1672 1674 1704 406
Size: 32 52 32 1120 30 2 30 2502
Fragments arranged by size:
2502 1120 52 32 32 30 30 2
HincII GTy'rAC
Cuts at: 839 839
Size: 3800
KasI G'GCGC_C
Cuts at: 1230 1478 1230
Size: 248 3552
KpnI G_GTAC'C
Cuts at: 820 820
Size: 3800
MluI A'CGCG_T
Cuts at: 3233 3233
Size: 3800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 533 659 2471 2655 533
Size: 126 1812 184 1678
Fragments arranged by size:
1812 1678 184 126
MscI TGG'CCA
Cuts at: 1561 1561
Size: 3800
MslI CAynn'nnrTG
Cuts at: 1789 1916 1789
Size: 127 3673
MspA1I CmG'CkG
Cuts at: 246 273 364 530 911 1225 1585 2598 2843
Size: 27 91 166 381 314 360 1013 245
Cuts at: 2843 246
Size: 1203
Fragments arranged by size:
1203 1013 381 360 314 245 166 91
27
NaeI GCC'GGC
Cuts at: 1981 1981
Size: 3800
NarI GG'CG_CC
Cuts at: 1231 1479 1231
Size: 248 3552
NcoI C'CATG_G
Cuts at: 107 107
Size: 3800
NgoMIV G'CCGG_C
Cuts at: 1979 1979
Size: 3800
NspI r_CATG'y
Cuts at: 853 1353 2260 853
Size: 500 907 2393
Fragments arranged by size:
2393 907 500
PciI A'CATG_T
Cuts at: 2256 2256
Size: 3800
PflMI CCAn_nnn'nTGG
Cuts at: 432 612 432
Size: 180 3620
PfoI T'CCnGG_A
Cuts at: 346 346
Size: 3800
PmeI GTTT'AAAC
Cuts at: 799 799
Size: 3800
PmlI CAC'GTG
Cuts at: 429 429
Size: 3800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2939 2971 2939
Size: 32 3768
PpuMI rG'GwC_Cy
Cuts at: 182 182
Size: 3800
PshAI GACnn'nnGTC
Cuts at: 559 559
Size: 3800
PspOMI G'GGCC_C
Cuts at: 385 574 385
Size: 189 3611
PstI C_TGCA'G
Cuts at: 847 1532 847
Size: 685 3115
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3800
PvuII CAG'CTG
Cuts at: 246 273 1225 1585 246
Size: 27 952 360 2461
Fragments arranged by size:
2461 952 360 27
RsrII CG'GwC_CG
Cuts at: 1995 1995
Size: 3800
SacI G_AGCT'C
Cuts at: 814 814
Size: 3800
SalI G'TCGA_C
Cuts at: 837 837
Size: 3800
SapI GCTCTTCn'nnn_
Cuts at: 1823 2033 1823
Size: 210 3590
SbfI CC_TGCA'GG
Cuts at: 847 847
Size: 3800
ScaI AGT'ACT
Cuts at: 3317 3317
Size: 3800
SfcI C'TryA_G
Cuts at: 33 843 1528 2521 2712 33
Size: 810 685 993 191 1121
Fragments arranged by size:
1121 993 810 685 191
SfoI GGC'GCC
Cuts at: 1232 1480 1232
Size: 248 3552
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3800
SmaI CCC'GGG
Cuts at: 822 822
Size: 3800
SmlI C'TyrA_G
Cuts at: 177 316 955 1870 2362 2624 177
Size: 139 639 915 492 262 1353
Fragments arranged by size:
1353 915 639 492 262 139
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3800
SphI G_CATG'C
Cuts at: 853 1353 853
Size: 500 3300
StyI C'CwwG_G
Cuts at: 107 766 934 1288 107
Size: 659 168 354 2619
Fragments arranged by size:
2619 659 354 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1383 1516 1975 2184 1383
Size: 133 459 209 2999
Fragments arranged by size:
2999 459 209 133
TatI w'GTAC_w
Cuts at: 293 3315 293
Size: 3022 778
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3317 3349 3317
Size: 32 3768
Tth111I GACn'n_nGTC
Cuts at: 1597 1597
Size: 3800
XbaI T'CTAG_A
Cuts at: 831 831
Size: 3800
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1295 1295
Size: 3800
XmaI C'CCGG_G
Cuts at: 820 820
Size: 3800
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BfrBI BglI BmgBI BmtI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI