Restriction map for
pF1KE0510
(Circular) (Six-base) MAPSORT of: pF1KE0510.seq Check: 2555 from: 1 to: 3818
pF1KE0510 3818 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 856 856
Size: 3818
Acc65I G'GTAC_C
Cuts at: 386 834 386
Size: 448 3370
AclI AA'CG_TT
Cuts at: 3658 3658
Size: 3818
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 312 339 408 1643 2075 2822 3090 312
Size: 27 69 1235 432 747 268 1040
Fragments arranged by size:
1235 1040 747 432 268 69 27
AfeI AGC'GCT
Cuts at: 296 296
Size: 3818
AflIII A'CryG_T
Cuts at: 1170 2274 3251 1170
Size: 1104 977 1737
Fragments arranged by size:
1737 1104 977
AgeI A'CCGG_T
Cuts at: 2206 2206
Size: 3818
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 256 288 256
Size: 32 3786
AlwNI CAG_nnn'CTG
Cuts at: 396 631 1123 2690 396
Size: 235 492 1567 1524
Fragments arranged by size:
1567 1524 492 235
ApaI G_GGCC'C
Cuts at: 256 256
Size: 3818
ApaLI G'TGCA_C
Cuts at: 2588 2588
Size: 3818
ApoI r'AATT_y
Cuts at: 688 746 822 3171 3427 688
Size: 58 76 2349 256 1079
Fragments arranged by size:
2349 1079 256 76 58
AseI AT'TA_AT
Cuts at: 20 20
Size: 3818
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3818
AvaI C'yCGr_G
Cuts at: 256 317 838 256
Size: 61 521 3236
Fragments arranged by size:
3236 521 61
BamHI G'GATC_C
Cuts at: 843 843
Size: 3818
BanI G'GyrC_C
Cuts at: 303 386 834 1248 1496 1531 303
Size: 83 448 414 248 35 2590
Fragments arranged by size:
2590 448 414 248 83 35
BanII G_rGCy'C
Cuts at: 256 832 1862 256
Size: 576 1030 2212
Fragments arranged by size:
2212 1030 576
BbeI G_GCGC'C
Cuts at: 1252 1500 1252
Size: 248 3570
BciVI GTATCCnnnnn_n'
Cuts at: 1716 2477 3818 1716
Size: 761 1341 1716
Fragments arranged by size:
1716 1341 761
BclI T'GATC_A
Cuts at: 1338 1338
Size: 3818
BglI GCCn_nnn'nGGC
Cuts at: 251 251
Size: 3818
BglII A'GATC_T
Cuts at: 1333 3370 1333
Size: 2037 1781
BlpI GC'TnA_GC
Cuts at: 184 930 184
Size: 746 3072
Bme1580I G_kGCm'C
Cuts at: 256 1443 1536 2592 256
Size: 1187 93 1056 1482
Fragments arranged by size:
1482 1187 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1030 1111 1446 3590 1030
Size: 81 335 2144 1258
Fragments arranged by size:
2144 1258 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2185 2185
Size: 3818
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 361
Size: 3818
Bpu10I CC'TnA_GC
Cuts at: 511 511
Size: 3818
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 994 1873 2365 2663 994
Size: 879 492 298 2149
Fragments arranged by size:
2149 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 171 440 3522 171
Size: 269 3082 467
Fragments arranged by size:
3082 467 269
BsaAI yAC'GTr
Cuts at: 1171 1171
Size: 3818
BsaBI GATnn'nnATC
Cuts at: 6 1332 3369 6
Size: 1326 2037 455
Fragments arranged by size:
2037 1326 455
BsaHI Gr'CG_yC
Cuts at: 1249 1497 2199 3695 1249
Size: 248 702 1496 1372
Fragments arranged by size:
1496 1372 702 248
BsaWI w'CCGG_w
Cuts at: 1007 1528 2206 2480 2627 3038 1007
Size: 521 678 274 147 411 1787
Fragments arranged by size:
1787 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 256 286 256
Size: 30 3788
BseYI C'CCAG_C
Cuts at: 1243 2001 2578 3074 1243
Size: 758 577 496 1987
Fragments arranged by size:
1987 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1360 1406 2614 104
Size: 1256 46 1208 1308
Fragments arranged by size:
1308 1256 1208 46
BsiHKAI G_wGCw'C
Cuts at: 832 1610 1800 2592 832
Size: 778 190 792 2058
Fragments arranged by size:
2058 792 778 190
BsmI GAATG_Cn'
Cuts at: 465 465
Size: 3818
Bsp1286I G_dGCh'C
Cuts at: 256 832 1443 1536 1610 1800 1862 2592 256
Size: 576 611 93 74 190 62 730 1482
Fragments arranged by size:
1482 730 611 576 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3038 3038
Size: 3818
BspHI T'CATG_A
Cuts at: 2994 2994
Size: 3818
BspMI ACCTGCnnnn'nnnn_
Cuts at: 868 1082 1384 1765 868
Size: 214 302 381 2921
Fragments arranged by size:
2921 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2110 2164 3648 3813 50
Size: 2060 54 1484 165 55
Fragments arranged by size:
2060 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1730 1730
Size: 3818
BsrFI r'CCGG_y
Cuts at: 1816 1997 2206 1816
Size: 181 209 3428
Fragments arranged by size:
3428 209 181
BssSI C'ACGA_G
Cuts at: 2089 2447 2089
Size: 358 3460
BstAPI GCAn_nnn'nTGC
Cuts at: 1195 1195
Size: 3818
BstBI TT'CG_AA
Cuts at: 2179 2179
Size: 3818
BstYI r'GATC_y
Cuts at: 1 843 1310 1333 1668 1914 2926 3012 3024
Size: 842 467 23 335 246 1012 86 12
Cuts at: 3024 3370 1
Size: 346 449
Fragments arranged by size:
1012 842 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 247 540 247
Size: 293 3525
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 206 1768 1949 2112 206
Size: 1562 181 163 1912
Fragments arranged by size:
1912 1562 181 163
BtsI GCAGTG_nn'
Cuts at: 714 1061 714
Size: 347 3471
DraI TTT'AAA
Cuts at: 817 817
Size: 3818
DrdI GACnn_nn'nnGTC
Cuts at: 1524 2382 1524
Size: 858 2960
EaeI y'GGCC_r
Cuts at: 154 1403 1577 1968 1995 3340 154
Size: 1249 174 391 27 1345 632
Fragments arranged by size:
1345 1249 632 391 174 27
EagI C'GGCC_G
Cuts at: 1403 1403
Size: 3818
EarI CTCTTCn'nnn_
Cuts at: 1841 2051 1841
Size: 210 3608
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2026 2336 2482 3628 2026
Size: 310 146 1146 2216
Fragments arranged by size:
2216 1146 310 146
EcoICRI GAG'CTC
Cuts at: 830 830
Size: 3818
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 312 339 361 408 1643 2075 2822 3090 312
Size: 27 22 47 1235 432 747 268 1040
Fragments arranged by size:
1235 1040 747 432 268 47 27 22
EcoO109I rG'GnC_Cy
Cuts at: 252 253 957 252
Size: 1 704 3113
Fragments arranged by size:
3113 704 1
EcoRI G'AATT_C
Cuts at: 822 822
Size: 3818
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1837 1837
Size: 3818
FspI TGC'GCA
Cuts at: 1599 1599
Size: 3818
HaeII r_GCGC'y
Cuts at: 298 1252 1500 2522 3501 298
Size: 954 248 1022 979 615
Fragments arranged by size:
1022 979 954 615 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 184 216 646 678 1660 1690 1692 1722 184
Size: 32 430 32 982 30 2 30 2280
Fragments arranged by size:
2280 982 430 32 32 30 30 2
HincII GTy'rAC
Cuts at: 857 857
Size: 3818
KasI G'GCGC_C
Cuts at: 1248 1496 1248
Size: 248 3570
KpnI G_GTAC'C
Cuts at: 390 838 390
Size: 448 3370
MluI A'CGCG_T
Cuts at: 3251 3251
Size: 3818
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 211 2489 2673 211
Size: 2278 184 1356
Fragments arranged by size:
2278 1356 184
MscI TGG'CCA
Cuts at: 1579 1579
Size: 3818
MslI CAynn'nnrTG
Cuts at: 1807 1934 1807
Size: 127 3691
MspA1I CmG'CkG
Cuts at: 227 929 1243 1603 2616 2861 227
Size: 702 314 360 1013 245 1184
Fragments arranged by size:
1184 1013 702 360 314 245
NaeI GCC'GGC
Cuts at: 1999 1999
Size: 3818
NarI GG'CG_CC
Cuts at: 1249 1497 1249
Size: 248 3570
NcoI C'CATG_G
Cuts at: 247 540 247
Size: 293 3525
NgoMIV G'CCGG_C
Cuts at: 1997 1997
Size: 3818
NspI r_CATG'y
Cuts at: 871 1371 2278 871
Size: 500 907 2411
Fragments arranged by size:
2411 907 500
PciI A'CATG_T
Cuts at: 2274 2274
Size: 3818
PmeI GTTT'AAAC
Cuts at: 817 817
Size: 3818
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 256 288 2957 2989 256
Size: 32 2669 32 1085
Fragments arranged by size:
2669 1085 32 32
PspOMI G'GGCC_C
Cuts at: 252 252
Size: 3818
PstI C_TGCA'G
Cuts at: 302 401 865 1550 302
Size: 99 464 685 2570
Fragments arranged by size:
2570 685 464 99
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3818
PvuII CAG'CTG
Cuts at: 1243 1603 1243
Size: 360 3458
RsrII CG'GwC_CG
Cuts at: 2013 2013
Size: 3818
SacI G_AGCT'C
Cuts at: 832 832
Size: 3818
SalI G'TCGA_C
Cuts at: 855 855
Size: 3818
SapI GCTCTTCn'nnn_
Cuts at: 1841 2051 1841
Size: 210 3608
SbfI CC_TGCA'GG
Cuts at: 865 865
Size: 3818
ScaI AGT'ACT
Cuts at: 3335 3335
Size: 3818
SfcI C'TryA_G
Cuts at: 33 298 397 861 1546 2539 2730 33
Size: 265 99 464 685 993 191 1121
Fragments arranged by size:
1121 993 685 464 265 191 99
SfiI GGCCn_nnn'nGGCC
Cuts at: 251 251
Size: 3818
SfoI GGC'GCC
Cuts at: 1250 1498 1250
Size: 248 3570
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3818
SmaI CCC'GGG
Cuts at: 258 840 258
Size: 582 3236
SmlI C'TyrA_G
Cuts at: 317 973 1888 2380 2642 317
Size: 656 915 492 262 1493
Fragments arranged by size:
1493 915 656 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3818
SphI G_CATG'C
Cuts at: 871 1371 871
Size: 500 3318
StyI C'CwwG_G
Cuts at: 247 540 740 952 1306 247
Size: 293 200 212 354 2759
Fragments arranged by size:
2759 354 293 212 200
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 428 1401 1534 1993 2202 428
Size: 973 133 459 209 2044
Fragments arranged by size:
2044 973 459 209 133
TatI w'GTAC_w
Cuts at: 3333 3333
Size: 3818
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3335 3367 3335
Size: 32 3786
Tth111I GACn'n_nGTC
Cuts at: 1615 1615
Size: 3818
XbaI T'CTAG_A
Cuts at: 849 849
Size: 3818
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1313 1313
Size: 3818
XhoI C'TCGA_G
Cuts at: 317 317
Size: 3818
XmaI C'CCGG_G
Cuts at: 256 838 256
Size: 582 3236
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI BseRI
BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SgrAI SnaBI SrfI
SspI StuI SwaI XmnI ZraI