Restriction map for
pF1KE0509
(Circular) (Six-base) MAPSORT of: pF1KE0509.seq Check: 7254 from: 1 to: 3623
pF1KE0509 3623 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 661 661
Size: 3623
Acc65I G'GTAC_C
Cuts at: 639 639
Size: 3623
AclI AA'CG_TT
Cuts at: 3463 3463
Size: 3623
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 114 211 1448 1880 2627 2895 114
Size: 97 1237 432 747 268 842
Fragments arranged by size:
1237 842 747 432 268 97
AflIII A'CryG_T
Cuts at: 975 2079 3056 975
Size: 1104 977 1542
Fragments arranged by size:
1542 1104 977
AgeI A'CCGG_T
Cuts at: 2011 2011
Size: 3623
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 518 518
Size: 3623
AlwNI CAG_nnn'CTG
Cuts at: 928 2495 928
Size: 1567 2056
ApaLI G'TGCA_C
Cuts at: 2393 2393
Size: 3623
ApoI r'AATT_y
Cuts at: 343 627 2976 3232 343
Size: 284 2349 256 734
Fragments arranged by size:
2349 734 284 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3623
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3623
AvaI C'yCGr_G
Cuts at: 643 643
Size: 3623
BamHI G'GATC_C
Cuts at: 648 648
Size: 3623
BanI G'GyrC_C
Cuts at: 639 1053 1301 1336 639
Size: 414 248 35 2926
Fragments arranged by size:
2926 414 248 35
BanII G_rGCy'C
Cuts at: 637 1667 637
Size: 1030 2593
BbeI G_GCGC'C
Cuts at: 1057 1305 1057
Size: 248 3375
BciVI GTATCCnnnnn_n'
Cuts at: 1521 2282 3623 1521
Size: 761 1341 1521
Fragments arranged by size:
1521 1341 761
BclI T'GATC_A
Cuts at: 1143 1143
Size: 3623
BglII A'GATC_T
Cuts at: 1138 3175 1138
Size: 2037 1586
BlpI GC'TnA_GC
Cuts at: 491 735 491
Size: 244 3379
Bme1580I G_kGCm'C
Cuts at: 1248 1341 2397 1248
Size: 93 1056 2474
Fragments arranged by size:
2474 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 293 835 916 1251 3395 293
Size: 542 81 335 2144 521
Fragments arranged by size:
2144 542 521 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1990 1990
Size: 3623
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 212
Size: 3623
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 799 1678 2170 2468 799
Size: 879 492 298 1954
Fragments arranged by size:
1954 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3327 3327
Size: 3623
BsaAI yAC'GTr
Cuts at: 976 976
Size: 3623
BsaBI GATnn'nnATC
Cuts at: 6 1137 3174 6
Size: 1131 2037 455
Fragments arranged by size:
2037 1131 455
BsaHI Gr'CG_yC
Cuts at: 1054 1302 2004 3500 1054
Size: 248 702 1496 1177
Fragments arranged by size:
1496 1177 702 248
BsaWI w'CCGG_w
Cuts at: 812 1333 2011 2285 2432 2843 812
Size: 521 678 274 147 411 1592
Fragments arranged by size:
1592 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1048 1806 2383 2879 1048
Size: 758 577 496 1792
Fragments arranged by size:
1792 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 123 1165 1211 2419 104
Size: 19 1042 46 1208 1308
Fragments arranged by size:
1308 1208 1042 46 19
BsiHKAI G_wGCw'C
Cuts at: 637 1415 1605 2397 637
Size: 778 190 792 1863
Fragments arranged by size:
1863 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 637 1248 1341 1415 1605 1667 2397 637
Size: 611 93 74 190 62 730 1863
Fragments arranged by size:
1863 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2843 2843
Size: 3623
BspHI T'CATG_A
Cuts at: 2799 2799
Size: 3623
BspMI ACCTGCnnnn'nnnn_
Cuts at: 673 887 1189 1570 673
Size: 214 302 381 2726
Fragments arranged by size:
2726 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1915 1969 3453 3618 50
Size: 1865 54 1484 165 55
Fragments arranged by size:
1865 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1535 1535
Size: 3623
BsrFI r'CCGG_y
Cuts at: 122 1621 1802 2011 122
Size: 1499 181 209 1734
Fragments arranged by size:
1734 1499 209 181
BssSI C'ACGA_G
Cuts at: 1894 2252 1894
Size: 358 3265
BstAPI GCAn_nnn'nTGC
Cuts at: 1000 1000
Size: 3623
BstBI TT'CG_AA
Cuts at: 1984 1984
Size: 3623
BstYI r'GATC_y
Cuts at: 1 648 1115 1138 1473 1719 2731 2817 2829
Size: 647 467 23 335 246 1012 86 12
Cuts at: 2829 3175 1
Size: 346 449
Fragments arranged by size:
1012 647 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 395 107
Size: 288 3335
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1573 1754 1917 1573
Size: 181 163 3279
Fragments arranged by size:
3279 181 163
BtsI GCAGTG_nn'
Cuts at: 509 866 509
Size: 357 3266
DraI TTT'AAA
Cuts at: 622 622
Size: 3623
DraIII CAC_nnn'GTG
Cuts at: 205 205
Size: 3623
DrdI GACnn_nn'nnGTC
Cuts at: 1329 2187 1329
Size: 858 2765
EaeI y'GGCC_r
Cuts at: 120 550 1208 1382 1773 1800 3145 120
Size: 430 658 174 391 27 1345 598
Fragments arranged by size:
1345 658 598 430 391 174 27
EagI C'GGCC_G
Cuts at: 120 1208 120
Size: 1088 2535
EarI CTCTTCn'nnn_
Cuts at: 331 599 1646 1856 331
Size: 268 1047 210 2098
Fragments arranged by size:
2098 1047 268 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1831 2141 2287 3433 1831
Size: 310 146 1146 2021
Fragments arranged by size:
2021 1146 310 146
EcoICRI GAG'CTC
Cuts at: 635 635
Size: 3623
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 114 211 212 1448 1880 2627 2895 114
Size: 97 1 1236 432 747 268 842
Fragments arranged by size:
1236 842 747 432 268 97 1
EcoO109I rG'GnC_Cy
Cuts at: 762 762
Size: 3623
EcoRI G'AATT_C
Cuts at: 627 627
Size: 3623
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1642 1642
Size: 3623
FseI GG_CCGG'CC
Cuts at: 126 126
Size: 3623
FspI TGC'GCA
Cuts at: 1404 1404
Size: 3623
HaeII r_GCGC'y
Cuts at: 1057 1305 2327 3306 1057
Size: 248 1022 979 1374
Fragments arranged by size:
1374 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1465 1495 1497 1527 1465
Size: 30 2 30 3561
Fragments arranged by size:
3561 30 30 2
HincII GTy'rAC
Cuts at: 288 662 288
Size: 374 3249
HindIII A'AGCT_T
Cuts at: 223 223
Size: 3623
HpaI GTT'AAC
Cuts at: 288 288
Size: 3623
KasI G'GCGC_C
Cuts at: 1053 1301 1053
Size: 248 3375
KpnI G_GTAC'C
Cuts at: 643 643
Size: 3623
MluI A'CGCG_T
Cuts at: 3056 3056
Size: 3623
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2294 2478 2294
Size: 184 3439
MscI TGG'CCA
Cuts at: 1384 1384
Size: 3623
MslI CAynn'nnrTG
Cuts at: 1612 1739 1612
Size: 127 3496
MspA1I CmG'CkG
Cuts at: 734 1048 1408 2421 2666 734
Size: 314 360 1013 245 1691
Fragments arranged by size:
1691 1013 360 314 245
NaeI GCC'GGC
Cuts at: 124 1804 124
Size: 1680 1943
NarI GG'CG_CC
Cuts at: 1054 1302 1054
Size: 248 3375
NcoI C'CATG_G
Cuts at: 107 395 107
Size: 288 3335
NgoMIV G'CCGG_C
Cuts at: 122 1802 122
Size: 1680 1943
NspI r_CATG'y
Cuts at: 499 676 1176 2083 499
Size: 177 500 907 2039
Fragments arranged by size:
2039 907 500 177
PciI A'CATG_T
Cuts at: 2079 2079
Size: 3623
PmeI GTTT'AAAC
Cuts at: 622 622
Size: 3623
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2762 2794 2762
Size: 32 3591
PsiI TTA'TAA
Cuts at: 572 572
Size: 3623
PstI C_TGCA'G
Cuts at: 670 1355 670
Size: 685 2938
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3623
PvuII CAG'CTG
Cuts at: 1048 1408 1048
Size: 360 3263
RsrII CG'GwC_CG
Cuts at: 1818 1818
Size: 3623
SacI G_AGCT'C
Cuts at: 637 637
Size: 3623
SalI G'TCGA_C
Cuts at: 660 660
Size: 3623
SapI GCTCTTCn'nnn_
Cuts at: 1646 1856 1646
Size: 210 3413
SbfI CC_TGCA'GG
Cuts at: 670 670
Size: 3623
ScaI AGT'ACT
Cuts at: 3140 3140
Size: 3623
SfcI C'TryA_G
Cuts at: 33 666 1351 2344 2535 33
Size: 633 685 993 191 1121
Fragments arranged by size:
1121 993 685 633 191
SfoI GGC'GCC
Cuts at: 1055 1303 1055
Size: 248 3375
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3623
SmaI CCC'GGG
Cuts at: 645 645
Size: 3623
SmlI C'TyrA_G
Cuts at: 778 1693 2185 2447 778
Size: 915 492 262 1954
Fragments arranged by size:
1954 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3623
SphI G_CATG'C
Cuts at: 676 1176 676
Size: 500 3123
StyI C'CwwG_G
Cuts at: 107 395 757 1111 107
Size: 288 362 354 2619
Fragments arranged by size:
2619 362 354 288
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1206 1339 1798 2007 1206
Size: 133 459 209 2822
Fragments arranged by size:
2822 459 209 133
TatI w'GTAC_w
Cuts at: 3138 3138
Size: 3623
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3140 3172 3140
Size: 32 3591
Tth111I GACn'n_nGTC
Cuts at: 1420 1420
Size: 3623
XbaI T'CTAG_A
Cuts at: 654 654
Size: 3623
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1118 1118
Size: 3623
XmaI C'CCGG_G
Cuts at: 643 643
Size: 3623
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FspAI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI