Restriction map for
pF1KE0501
(Circular) (Six-base) MAPSORT of: pF1KE0501.seq Check: 4321 from: 1 to: 3728
pF1KE0501 3728 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 766 766
Size: 3728
Acc65I G'GTAC_C
Cuts at: 744 744
Size: 3728
AclI AA'CG_TT
Cuts at: 722 3568 722
Size: 2846 882
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 1553 1985 2732 3000 250
Size: 1303 432 747 268 978
Fragments arranged by size:
1303 978 747 432 268
AflIII A'CryG_T
Cuts at: 1080 2184 3161 1080
Size: 1104 977 1647
Fragments arranged by size:
1647 1104 977
AgeI A'CCGG_T
Cuts at: 2116 2116
Size: 3728
AleI CACnn'nnGTG
Cuts at: 698 698
Size: 3728
AlwNI CAG_nnn'CTG
Cuts at: 259 600 1033 2600 259
Size: 341 433 1567 1387
Fragments arranged by size:
1567 1387 433 341
ApaI G_GGCC'C
Cuts at: 218 645 218
Size: 427 3301
ApaLI G'TGCA_C
Cuts at: 2498 2498
Size: 3728
ApoI r'AATT_y
Cuts at: 732 3081 3337 732
Size: 2349 256 1123
Fragments arranged by size:
2349 1123 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3728
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3728
AvaI C'yCGr_G
Cuts at: 748 748
Size: 3728
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 580 613 580
Size: 33 3695
BamHI G'GATC_C
Cuts at: 753 753
Size: 3728
BanI G'GyrC_C
Cuts at: 692 703 744 1158 1406 1441 692
Size: 11 41 414 248 35 2979
Fragments arranged by size:
2979 414 248 41 35 11
BanII G_rGCy'C
Cuts at: 209 218 645 742 1772 209
Size: 9 427 97 1030 2165
Fragments arranged by size:
2165 1030 427 97 9
BbeI G_GCGC'C
Cuts at: 1162 1410 1162
Size: 248 3480
BbvCI CC'TCA_GC
Cuts at: 388 388
Size: 3728
BciVI GTATCCnnnnn_n'
Cuts at: 685 1626 2387 3728 685
Size: 941 761 1341 685
Fragments arranged by size:
1341 941 761 685
BclI T'GATC_A
Cuts at: 1248 1248
Size: 3728
BglII A'GATC_T
Cuts at: 1243 3280 1243
Size: 2037 1691
BlpI GC'TnA_GC
Cuts at: 840 840
Size: 3728
Bme1580I G_kGCm'C
Cuts at: 218 645 1353 1446 2502 218
Size: 427 708 93 1056 1444
Fragments arranged by size:
1444 1056 708 427 93
BmrI ACTGGGnnnn_n'
Cuts at: 940 1021 1356 3500 940
Size: 81 335 2144 1168
Fragments arranged by size:
2144 1168 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2095 2095
Size: 3728
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 625
Size: 3728
Bpu10I CC'TnA_GC
Cuts at: 388 388
Size: 3728
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 904 1783 2275 2573 904
Size: 879 492 298 2059
Fragments arranged by size:
2059 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3432 3432
Size: 3728
BsaAI yAC'GTr
Cuts at: 557 1081 557
Size: 524 3204
BsaBI GATnn'nnATC
Cuts at: 6 166 475 1242 3279 6
Size: 160 309 767 2037 455
Fragments arranged by size:
2037 767 455 309 160
BsaHI Gr'CG_yC
Cuts at: 1159 1407 2109 3605 1159
Size: 248 702 1496 1282
Fragments arranged by size:
1496 1282 702 248
BsaWI w'CCGG_w
Cuts at: 917 1438 2116 2390 2537 2948 917
Size: 521 678 274 147 411 1697
Fragments arranged by size:
1697 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 514 544 514
Size: 30 3698
BseYI C'CCAG_C
Cuts at: 350 496 1153 1911 2488 2984 350
Size: 146 657 758 577 496 1094
Fragments arranged by size:
1094 758 657 577 496 146
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 565 679 289
Size: 276 114 3338
Fragments arranged by size:
3338 276 114
BsiEI CG_ry'CG
Cuts at: 104 1270 1316 2524 104
Size: 1166 46 1208 1308
Fragments arranged by size:
1308 1208 1166 46
BsiHKAI G_wGCw'C
Cuts at: 209 428 742 1520 1710 2502 209
Size: 219 314 778 190 792 1435
Fragments arranged by size:
1435 792 778 314 219 190
Bsp1286I G_dGCh'C
Cuts at: 209 218 428 645 742 1353 1446 1520 1710
Size: 9 210 217 97 611 93 74 190
Cuts at: 1710 1772 2502 209
Size: 62 730 1435
Fragments arranged by size:
1435 730 611 217 210 190 97 93
74 62 9
BspEI T'CCGG_A
Cuts at: 2948 2948
Size: 3728
BspHI T'CATG_A
Cuts at: 2904 2904
Size: 3728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 244 608 778 992 1294 1675 244
Size: 364 170 214 302 381 2297
Fragments arranged by size:
2297 381 364 302 214 170
BsrBI CCG'CTC
Cuts at: 50 2020 2074 3558 3723 50
Size: 1970 54 1484 165 55
Fragments arranged by size:
1970 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1640 1640
Size: 3728
BsrFI r'CCGG_y
Cuts at: 1726 1907 2116 1726
Size: 181 209 3338
Fragments arranged by size:
3338 209 181
BssSI C'ACGA_G
Cuts at: 1999 2357 1999
Size: 358 3370
BstAPI GCAn_nnn'nTGC
Cuts at: 600 666 1105 600
Size: 66 439 3223
Fragments arranged by size:
3223 439 66
BstBI TT'CG_AA
Cuts at: 2089 2089
Size: 3728
BstXI CCAn_nnnn'nTGG
Cuts at: 411 511 411
Size: 100 3628
BstYI r'GATC_y
Cuts at: 1 753 1220 1243 1578 1824 2836 2922 2934
Size: 752 467 23 335 246 1012 86 12
Cuts at: 2934 3280 1
Size: 346 449
Fragments arranged by size:
1012 752 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 397 397
Size: 3728
BtgI C'CryG_G
Cuts at: 107 510 585 107
Size: 403 75 3250
Fragments arranged by size:
3250 403 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1678 1859 2022 1678
Size: 181 163 3384
Fragments arranged by size:
3384 181 163
BtsI GCAGTG_nn'
Cuts at: 694 971 694
Size: 277 3451
DraI TTT'AAA
Cuts at: 727 727
Size: 3728
DrdI GACnn_nn'nnGTC
Cuts at: 1434 2292 1434
Size: 858 2870
EaeI y'GGCC_r
Cuts at: 1313 1487 1878 1905 3250 1313
Size: 174 391 27 1345 1791
Fragments arranged by size:
1791 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1313 1313
Size: 3728
EarI CTCTTCn'nnn_
Cuts at: 1751 1961 1751
Size: 210 3518
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1936 2246 2392 3538 1936
Size: 310 146 1146 2126
Fragments arranged by size:
2126 1146 310 146
EcoICRI GAG'CTC
Cuts at: 207 740 207
Size: 533 3195
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 625 1553 1985 2732 3000 250
Size: 375 928 432 747 268 978
Fragments arranged by size:
978 928 747 432 375 268
EcoNI CCTnn'n_nnAGG
Cuts at: 268 268
Size: 3728
EcoO109I rG'GnC_Cy
Cuts at: 215 641 642 867 215
Size: 426 1 225 3076
Fragments arranged by size:
3076 426 225 1
EcoRI G'AATT_C
Cuts at: 732 732
Size: 3728
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1747 1747
Size: 3728
FspI TGC'GCA
Cuts at: 1509 1509
Size: 3728
HaeII r_GCGC'y
Cuts at: 1162 1410 2432 3411 1162
Size: 248 1022 979 1479
Fragments arranged by size:
1479 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 514 546 1570 1600 1602 1632 514
Size: 32 1024 30 2 30 2610
Fragments arranged by size:
2610 1024 32 30 30 2
HincII GTy'rAC
Cuts at: 767 767
Size: 3728
KasI G'GCGC_C
Cuts at: 1158 1406 1158
Size: 248 3480
KpnI G_GTAC'C
Cuts at: 748 748
Size: 3728
MluI A'CGCG_T
Cuts at: 3161 3161
Size: 3728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 743 2399 2583 743
Size: 1656 184 1888
Fragments arranged by size:
1888 1656 184
MscI TGG'CCA
Cuts at: 1489 1489
Size: 3728
MslI CAynn'nnrTG
Cuts at: 200 409 509 698 1717 1844 200
Size: 209 100 189 1019 127 2084
Fragments arranged by size:
2084 1019 209 189 127 100
MspA1I CmG'CkG
Cuts at: 350 839 1153 1513 2526 2771 350
Size: 489 314 360 1013 245 1307
Fragments arranged by size:
1307 1013 489 360 314 245
NaeI GCC'GGC
Cuts at: 1909 1909
Size: 3728
NarI GG'CG_CC
Cuts at: 1159 1407 1159
Size: 248 3480
NcoI C'CATG_G
Cuts at: 107 510 107
Size: 403 3325
NgoMIV G'CCGG_C
Cuts at: 1907 1907
Size: 3728
NspI r_CATG'y
Cuts at: 687 781 1281 2188 687
Size: 94 500 907 2227
Fragments arranged by size:
2227 907 500 94
PasI CC'CwG_GG
Cuts at: 281 281
Size: 3728
PciI A'CATG_T
Cuts at: 2184 2184
Size: 3728
PmeI GTTT'AAAC
Cuts at: 727 727
Size: 3728
PmlI CAC'GTG
Cuts at: 557 557
Size: 3728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2867 2899 2867
Size: 32 3696
PspOMI G'GGCC_C
Cuts at: 214 641 214
Size: 427 3301
PstI C_TGCA'G
Cuts at: 596 775 1460 596
Size: 179 685 2864
Fragments arranged by size:
2864 685 179
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3728
PvuII CAG'CTG
Cuts at: 1153 1513 1153
Size: 360 3368
RsrII CG'GwC_CG
Cuts at: 1923 1923
Size: 3728
SacI G_AGCT'C
Cuts at: 209 742 209
Size: 533 3195
SalI G'TCGA_C
Cuts at: 765 765
Size: 3728
SapI GCTCTTCn'nnn_
Cuts at: 1751 1961 1751
Size: 210 3518
SbfI CC_TGCA'GG
Cuts at: 775 775
Size: 3728
ScaI AGT'ACT
Cuts at: 3245 3245
Size: 3728
SfcI C'TryA_G
Cuts at: 33 592 771 1456 2449 2640 33
Size: 559 179 685 993 191 1121
Fragments arranged by size:
1121 993 685 559 191 179
SfoI GGC'GCC
Cuts at: 1160 1408 1160
Size: 248 3480
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3728
SmaI CCC'GGG
Cuts at: 750 750
Size: 3728
SmlI C'TyrA_G
Cuts at: 883 1798 2290 2552 883
Size: 915 492 262 2059
Fragments arranged by size:
2059 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3728
SphI G_CATG'C
Cuts at: 781 1281 781
Size: 500 3228
StyI C'CwwG_G
Cuts at: 107 510 862 1216 107
Size: 403 352 354 2619
Fragments arranged by size:
2619 403 354 352
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 273 1311 1444 1903 2112 273
Size: 1038 133 459 209 1889
Fragments arranged by size:
1889 1038 459 209 133
TatI w'GTAC_w
Cuts at: 3243 3243
Size: 3728
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 269 301 3245 3277 269
Size: 32 2944 32 720
Fragments arranged by size:
2944 720 32 32
Tth111I GACn'n_nGTC
Cuts at: 1525 1525
Size: 3728
XbaI T'CTAG_A
Cuts at: 759 759
Size: 3728
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1223 1223
Size: 3728
XmaI C'CCGG_G
Cuts at: 748 748
Size: 3728
XmnI GAAnn'nnTTC
Cuts at: 185 185
Size: 3728
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PmeI PmlI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BbsI BcgI BfrBI BglI BmgBI BmtI BseRI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PfoI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI