Restriction map for
pF1KE0494
(Circular) (Six-base) MAPSORT of: pF1KE0494.seq Check: 2045 from: 1 to: 3767
pF1KE0494 3767 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 189 433 189
Size: 244 3523
AccI GT'mk_AC
Cuts at: 805 805
Size: 3767
Acc65I G'GTAC_C
Cuts at: 783 783
Size: 3767
AclI AA'CG_TT
Cuts at: 3607 3607
Size: 3767
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1592 2024 2771 3039 1592
Size: 432 747 268 2320
Fragments arranged by size:
2320 747 432 268
AfeI AGC'GCT
Cuts at: 596 596
Size: 3767
AflIII A'CryG_T
Cuts at: 1119 2223 3200 1119
Size: 1104 977 1686
Fragments arranged by size:
1686 1104 977
AgeI A'CCGG_T
Cuts at: 2155 2155
Size: 3767
AleI CACnn'nnGTG
Cuts at: 334 427 334
Size: 93 3674
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 352 352
Size: 3767
AlwNI CAG_nnn'CTG
Cuts at: 1072 2639 1072
Size: 1567 2200
ApaI G_GGCC'C
Cuts at: 442 442
Size: 3767
ApaLI G'TGCA_C
Cuts at: 417 2537 417
Size: 2120 1647
ApoI r'AATT_y
Cuts at: 771 3120 3376 771
Size: 2349 256 1162
Fragments arranged by size:
2349 1162 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3767
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3767
AvaI C'yCGr_G
Cuts at: 435 787 435
Size: 352 3415
BamHI G'GATC_C
Cuts at: 792 792
Size: 3767
BanI G'GyrC_C
Cuts at: 658 783 1197 1445 1480 658
Size: 125 414 248 35 2945
Fragments arranged by size:
2945 414 248 125 35
BanII G_rGCy'C
Cuts at: 442 781 1811 442
Size: 339 1030 2398
Fragments arranged by size:
2398 1030 339
BbeI G_GCGC'C
Cuts at: 662 1201 1449 662
Size: 539 248 2980
Fragments arranged by size:
2980 539 248
BbvCI CC'TCA_GC
Cuts at: 688 688
Size: 3767
BciVI GTATCCnnnnn_n'
Cuts at: 1665 2426 3767 1665
Size: 761 1341 1665
Fragments arranged by size:
1665 1341 761
BclI T'GATC_A
Cuts at: 1287 1287
Size: 3767
BglI GCCn_nnn'nGGC
Cuts at: 155 195 720 155
Size: 40 525 3202
Fragments arranged by size:
3202 525 40
BglII A'GATC_T
Cuts at: 1282 3319 1282
Size: 2037 1730
BlpI GC'TnA_GC
Cuts at: 879 879
Size: 3767
Bme1580I G_kGCm'C
Cuts at: 290 421 442 566 1392 1485 2541 290
Size: 131 21 124 826 93 1056 1516
Fragments arranged by size:
1516 1056 826 131 124 93 21
BmrI ACTGGGnnnn_n'
Cuts at: 979 1060 1395 3539 979
Size: 81 335 2144 1207
Fragments arranged by size:
2144 1207 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 493 2134 493
Size: 1641 2126
Bpu10I CC'TnA_GC
Cuts at: 688 688
Size: 3767
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 943 1822 2314 2612 943
Size: 879 492 298 2098
Fragments arranged by size:
2098 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3471 3471
Size: 3767
BsaAI yAC'GTr
Cuts at: 1120 1120
Size: 3767
BsaBI GATnn'nnATC
Cuts at: 6 1281 3318 6
Size: 1275 2037 455
Fragments arranged by size:
2037 1275 455
BsaHI Gr'CG_yC
Cuts at: 659 1198 1446 2148 3644 659
Size: 539 248 702 1496 782
Fragments arranged by size:
1496 782 702 539 248
BsaWI w'CCGG_w
Cuts at: 956 1477 2155 2429 2576 2987 956
Size: 521 678 274 147 411 1736
Fragments arranged by size:
1736 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 158 585 608 1192 1950 2527 3023 158
Size: 427 23 584 758 577 496 902
Fragments arranged by size:
902 758 584 577 496 427 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 358 654 295
Size: 63 296 3408
Fragments arranged by size:
3408 296 63
BsiEI CG_ry'CG
Cuts at: 104 678 1309 1355 2563 104
Size: 574 631 46 1208 1308
Fragments arranged by size:
1308 1208 631 574 46
BsiHKAI G_wGCw'C
Cuts at: 283 331 421 479 781 1559 1749 2541 283
Size: 48 90 58 302 778 190 792 1509
Fragments arranged by size:
1509 792 778 302 190 90 58 48
Bsp1286I G_dGCh'C
Cuts at: 283 290 331 421 442 479 566 781 1392
Size: 7 41 90 21 37 87 215 611
Cuts at: 1392 1485 1559 1749 1811 2541 283
Size: 93 74 190 62 730 1509
Fragments arranged by size:
1509 730 611 215 190 93 90 87
74 62 41 37 21 7
BspEI T'CCGG_A
Cuts at: 2987 2987
Size: 3767
BspHI T'CATG_A
Cuts at: 2943 2943
Size: 3767
BspMI ACCTGCnnnn'nnnn_
Cuts at: 189 433 817 1031 1333 1714 189
Size: 244 384 214 302 381 2242
Fragments arranged by size:
2242 384 381 302 244 214
BsrBI CCG'CTC
Cuts at: 50 2059 2113 3597 3762 50
Size: 2009 54 1484 165 55
Fragments arranged by size:
2009 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1679 1679
Size: 3767
BsrFI r'CCGG_y
Cuts at: 673 733 1765 1946 2155 673
Size: 60 1032 181 209 2285
Fragments arranged by size:
2285 1032 209 181 60
BsrGI T'GTAC_A
Cuts at: 599 599
Size: 3767
BssHII G'CGCG_C
Cuts at: 410 410
Size: 3767
BssSI C'ACGA_G
Cuts at: 505 2038 2396 505
Size: 1533 358 1876
Fragments arranged by size:
1876 1533 358
BstAPI GCAn_nnn'nTGC
Cuts at: 425 1144 425
Size: 719 3048
BstBI TT'CG_AA
Cuts at: 2128 2128
Size: 3767
BstEII G'GTnAC_C
Cuts at: 201 201
Size: 3767
BstXI CCAn_nnnn'nTGG
Cuts at: 429 518 429
Size: 89 3678
BstYI r'GATC_y
Cuts at: 1 128 792 1259 1282 1617 1863 2875 2961
Size: 127 664 467 23 335 246 1012 86
Cuts at: 2961 2973 3319 1
Size: 12 346 449
Fragments arranged by size:
1012 664 467 449 346 335 246 127
86 23 12
BtgI C'CryG_G
Cuts at: 107 715 725 107
Size: 608 10 3149
Fragments arranged by size:
3149 608 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1717 1898 2061 1717
Size: 181 163 3423
Fragments arranged by size:
3423 181 163
BtsI GCAGTG_nn'
Cuts at: 301 421 1010 301
Size: 120 589 3058
Fragments arranged by size:
3058 589 120
DraI TTT'AAA
Cuts at: 766 766
Size: 3767
DrdI GACnn_nn'nnGTC
Cuts at: 1473 2331 1473
Size: 858 2909
EaeI y'GGCC_r
Cuts at: 443 614 675 728 1352 1526 1917 1944 3289
Size: 171 61 53 624 174 391 27 1345
Cuts at: 3289 443
Size: 921
Fragments arranged by size:
1345 921 624 391 174 171 61 53
27
EagI C'GGCC_G
Cuts at: 675 1352 675
Size: 677 3090
EarI CTCTTCn'nnn_
Cuts at: 397 1790 2000 397
Size: 1393 210 2164
Fragments arranged by size:
2164 1393 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1975 2285 2431 3577 1975
Size: 310 146 1146 2165
Fragments arranged by size:
2165 1146 310 146
EcoICRI GAG'CTC
Cuts at: 779 779
Size: 3767
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1592 2024 2771 3039 1592
Size: 432 747 268 2320
Fragments arranged by size:
2320 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 906 906
Size: 3767
EcoRI G'AATT_C
Cuts at: 771 771
Size: 3767
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1786 1786
Size: 3767
FspI TGC'GCA
Cuts at: 1548 1548
Size: 3767
HaeII r_GCGC'y
Cuts at: 355 368 505 598 662 715 1201 1449 2471
Size: 13 137 93 64 53 486 248 1022
Cuts at: 2471 3450 355
Size: 979 672
Fragments arranged by size:
1022 979 672 486 248 137 93 64
53 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1609 1639 1641 1671 1609
Size: 30 2 30 3705
Fragments arranged by size:
3705 30 30 2
HincII GTy'rAC
Cuts at: 806 806
Size: 3767
KasI G'GCGC_C
Cuts at: 658 1197 1445 658
Size: 539 248 2980
Fragments arranged by size:
2980 539 248
KpnI G_GTAC'C
Cuts at: 787 787
Size: 3767
MluI A'CGCG_T
Cuts at: 3200 3200
Size: 3767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 104 2438 2622 104
Size: 2334 184 1249
Fragments arranged by size:
2334 1249 184
MscI TGG'CCA
Cuts at: 1528 1528
Size: 3767
MslI CAynn'nnrTG
Cuts at: 334 427 1756 1883 334
Size: 93 1329 127 2218
Fragments arranged by size:
2218 1329 127 93
MspA1I CmG'CkG
Cuts at: 717 878 1192 1552 2565 2810 717
Size: 161 314 360 1013 245 1674
Fragments arranged by size:
1674 1013 360 314 245 161
NaeI GCC'GGC
Cuts at: 1948 1948
Size: 3767
NarI GG'CG_CC
Cuts at: 659 1198 1446 659
Size: 539 248 2980
Fragments arranged by size:
2980 539 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3767
NgoMIV G'CCGG_C
Cuts at: 1946 1946
Size: 3767
NspI r_CATG'y
Cuts at: 294 820 1320 2227 294
Size: 526 500 907 1834
Fragments arranged by size:
1834 907 526 500
PciI A'CATG_T
Cuts at: 2223 2223
Size: 3767
PfoI T'CCnGG_A
Cuts at: 212 212
Size: 3767
PmeI GTTT'AAAC
Cuts at: 766 766
Size: 3767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2906 2938 2906
Size: 32 3735
PspOMI G'GGCC_C
Cuts at: 438 438
Size: 3767
PstI C_TGCA'G
Cuts at: 814 1499 814
Size: 685 3082
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3767
PvuII CAG'CTG
Cuts at: 1192 1552 1192
Size: 360 3407
RsrII CG'GwC_CG
Cuts at: 1962 1962
Size: 3767
SacI G_AGCT'C
Cuts at: 781 781
Size: 3767
SacII CC_GC'GG
Cuts at: 718 718
Size: 3767
SalI G'TCGA_C
Cuts at: 804 804
Size: 3767
SapI GCTCTTCn'nnn_
Cuts at: 397 1790 2000 397
Size: 1393 210 2164
Fragments arranged by size:
2164 1393 210
SbfI CC_TGCA'GG
Cuts at: 814 814
Size: 3767
ScaI AGT'ACT
Cuts at: 706 3284 706
Size: 2578 1189
SfcI C'TryA_G
Cuts at: 33 810 1495 2488 2679 33
Size: 777 685 993 191 1121
Fragments arranged by size:
1121 993 777 685 191
SfoI GGC'GCC
Cuts at: 660 1199 1447 660
Size: 539 248 2980
Fragments arranged by size:
2980 539 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3767
SgrAI Cr'CCGG_yG
Cuts at: 733 733
Size: 3767
SmaI CCC'GGG
Cuts at: 437 789 437
Size: 352 3415
SmlI C'TyrA_G
Cuts at: 922 1837 2329 2591 922
Size: 915 492 262 2098
Fragments arranged by size:
2098 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3767
SphI G_CATG'C
Cuts at: 820 1320 820
Size: 500 3267
StyI C'CwwG_G
Cuts at: 107 446 901 1255 107
Size: 339 455 354 2619
Fragments arranged by size:
2619 455 354 339
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1350 1483 1942 2151 1350
Size: 133 459 209 2966
Fragments arranged by size:
2966 459 209 133
TatI w'GTAC_w
Cuts at: 599 704 3282 599
Size: 105 2578 1084
Fragments arranged by size:
2578 1084 105
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 663 695 3284 3316 663
Size: 32 2589 32 1114
Fragments arranged by size:
2589 1114 32 32
Tth111I GACn'n_nGTC
Cuts at: 1564 1564
Size: 3767
XbaI T'CTAG_A
Cuts at: 798 798
Size: 3767
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 453 1262 453
Size: 809 2958
XmaI C'CCGG_G
Cuts at: 435 787 435
Size: 352 3415
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BbsI
BcgI BfrBI BmgBI BmtI BpmI BsaXI BseRI BsiWI
BsmI BsmBI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PpuMI
PshAI PsiI PspXI PsrI SanDI SexAI SfiI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI