Restriction map for
pF1KE0493
(Circular) (Six-base) MAPSORT of: pF1KE0493.seq Check: 2419 from: 1 to: 4298
pF1KE0493 4298 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 386 386
Size: 4298
AccI GT'mk_AC
Cuts at: 799 1336 799
Size: 537 3761
Acc65I G'GTAC_C
Cuts at: 689 1314 689
Size: 625 3673
AclI AA'CG_TT
Cuts at: 4138 4138
Size: 4298
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 781 2123 2555 3302 3570 781
Size: 1342 432 747 268 1509
Fragments arranged by size:
1509 1342 747 432 268
AflIII A'CryG_T
Cuts at: 1650 2754 3731 1650
Size: 1104 977 2217
Fragments arranged by size:
2217 1104 977
AgeI A'CCGG_T
Cuts at: 2686 2686
Size: 4298
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 658 658
Size: 4298
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 533 565 533
Size: 32 4266
AlwNI CAG_nnn'CTG
Cuts at: 711 1603 3170 711
Size: 892 1567 1839
Fragments arranged by size:
1839 1567 892
ApaI G_GGCC'C
Cuts at: 506 506
Size: 4298
ApaLI G'TGCA_C
Cuts at: 3068 3068
Size: 4298
ApoI r'AATT_y
Cuts at: 1207 1302 3651 3907 1207
Size: 95 2349 256 1598
Fragments arranged by size:
2349 1598 256 95
AscI GG'CGCG_CC
Cuts at: 216 216
Size: 4298
AseI AT'TA_AT
Cuts at: 20 20
Size: 4298
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4298
AvaI C'yCGr_G
Cuts at: 304 401 1318 304
Size: 97 917 3284
Fragments arranged by size:
3284 917 97
BamHI G'GATC_C
Cuts at: 1323 1323
Size: 4298
BanI G'GyrC_C
Cuts at: 263 290 689 1314 1728 1976 2011 263
Size: 27 399 625 414 248 35 2550
Fragments arranged by size:
2550 625 414 399 248 35 27
BanII G_rGCy'C
Cuts at: 401 506 662 1312 2342 401
Size: 105 156 650 1030 2357
Fragments arranged by size:
2357 1030 650 156 105
BbeI G_GCGC'C
Cuts at: 294 1732 1980 294
Size: 1438 248 2612
Fragments arranged by size:
2612 1438 248
BbsI GAAGACnn'nnnn_
Cuts at: 513 810 513
Size: 297 4001
BbvCI CC'TCA_GC
Cuts at: 313 340 313
Size: 27 4271
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1027 1061 1027
Size: 34 4264
BciVI GTATCCnnnnn_n'
Cuts at: 2196 2957 4298 2196
Size: 761 1341 2196
Fragments arranged by size:
2196 1341 761
BclI T'GATC_A
Cuts at: 1818 1818
Size: 4298
BglI GCCn_nnn'nGGC
Cuts at: 112 666 112
Size: 554 3744
BglII A'GATC_T
Cuts at: 1813 3850 1813
Size: 2037 2261
BlpI GC'TnA_GC
Cuts at: 1410 1410
Size: 4298
Bme1580I G_kGCm'C
Cuts at: 193 506 1923 2016 3072 193
Size: 313 1417 93 1056 1419
Fragments arranged by size:
1419 1417 1056 313 93
BmrI ACTGGGnnnn_n'
Cuts at: 1224 1510 1591 1926 4070 1224
Size: 286 81 335 2144 1452
Fragments arranged by size:
2144 1452 335 286 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1101 2665 1101
Size: 1564 2734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 1168 766
Size: 402 3896
Bpu10I CC'TnA_GC
Cuts at: 313 340 313
Size: 27 4271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 557 1007 1474 2353 2845 3143 557
Size: 450 467 879 492 298 1712
Fragments arranged by size:
1712 879 492 467 450 298
BsaI GGTCTCn'nnnn_
Cuts at: 1072 4002 1072
Size: 2930 1368
BsaAI yAC'GTr
Cuts at: 1651 1651
Size: 4298
BsaBI GATnn'nnATC
Cuts at: 6 1812 3849 6
Size: 1806 2037 455
Fragments arranged by size:
2037 1806 455
BsaHI Gr'CG_yC
Cuts at: 239 291 1729 1977 2679 4175 239
Size: 52 1438 248 702 1496 362
Fragments arranged by size:
1496 1438 702 362 248 52
BsaWI w'CCGG_w
Cuts at: 1487 2008 2686 2960 3107 3518 1487
Size: 521 678 274 147 411 2267
Fragments arranged by size:
2267 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 322 409 1108 1275 322
Size: 87 699 167 3345
Fragments arranged by size:
3345 699 167 87
BseYI C'CCAG_C
Cuts at: 382 498 506 1723 2481 3058 3554 382
Size: 116 8 1217 758 577 496 1126
Fragments arranged by size:
1217 1126 758 577 496 116 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 319
Size: 4298
BsiEI CG_ry'CG
Cuts at: 104 116 496 1840 1886 3094 104
Size: 12 380 1344 46 1208 1308
Fragments arranged by size:
1344 1308 1208 380 46 12
BsiHKAI G_wGCw'C
Cuts at: 1312 2090 2280 3072 1312
Size: 778 190 792 2538
Fragments arranged by size:
2538 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 193 401 506 662 1312 1923 2016 2090 2280
Size: 208 105 156 650 611 93 74 190
Cuts at: 2280 2342 3072 193
Size: 62 730 1419
Fragments arranged by size:
1419 730 650 611 208 190 156 105
93 74 62
BspEI T'CCGG_A
Cuts at: 3518 3518
Size: 4298
BspHI T'CATG_A
Cuts at: 3474 3474
Size: 4298
BspMI ACCTGCnnnn'nnnn_
Cuts at: 356 386 719 749 1348 1562 1864 2245 356
Size: 30 333 30 599 214 302 381 2409
Fragments arranged by size:
2409 599 381 333 302 214 30 30
BsrBI CCG'CTC
Cuts at: 50 332 492 687 2590 2644 4128 4293 50
Size: 282 160 195 1903 54 1484 165 55
Fragments arranged by size:
1903 1484 282 195 165 160 55 54
BsrDI GCAATG_nn'
Cuts at: 605 2210 605
Size: 1605 2693
BsrFI r'CCGG_y
Cuts at: 533 2296 2477 2686 533
Size: 1763 181 209 2145
Fragments arranged by size:
2145 1763 209 181
BsrGI T'GTAC_A
Cuts at: 752 752
Size: 4298
BssHII G'CGCG_C
Cuts at: 216 216
Size: 4298
BssSI C'ACGA_G
Cuts at: 976 2569 2927 976
Size: 1593 358 2347
Fragments arranged by size:
2347 1593 358
BstAPI GCAn_nnn'nTGC
Cuts at: 607 1675 607
Size: 1068 3230
BstBI TT'CG_AA
Cuts at: 2659 2659
Size: 4298
BstXI CCAn_nnnn'nTGG
Cuts at: 618 618
Size: 4298
BstYI r'GATC_y
Cuts at: 1 701 1323 1790 1813 2148 2394 3406 3492
Size: 700 622 467 23 335 246 1012 86
Cuts at: 3492 3504 3850 1
Size: 12 346 449
Fragments arranged by size:
1012 700 622 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 781 781
Size: 4298
BtgI C'CryG_G
Cuts at: 107 680 107
Size: 573 3725
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 791 2248 2429 2592 791
Size: 1457 181 163 2497
Fragments arranged by size:
2497 1457 181 163
BtsI GCAGTG_nn'
Cuts at: 1541 1541
Size: 4298
DraI TTT'AAA
Cuts at: 1297 1297
Size: 4298
DrdI GACnn_nn'nnGTC
Cuts at: 2004 2862 2004
Size: 858 3440
EaeI y'GGCC_r
Cuts at: 113 131 161 559 1883 2057 2448 2475 3820
Size: 18 30 398 1324 174 391 27 1345
Cuts at: 3820 113
Size: 591
Fragments arranged by size:
1345 1324 591 398 391 174 30 27
18
EagI C'GGCC_G
Cuts at: 113 1883 113
Size: 1770 2528
EarI CTCTTCn'nnn_
Cuts at: 358 2321 2531 358
Size: 1963 210 2125
Fragments arranged by size:
2125 1963 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2506 2816 2962 4108 2506
Size: 310 146 1146 2696
Fragments arranged by size:
2696 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1310 1310
Size: 4298
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 781 1168 2123 2555 3302 3570 766
Size: 15 387 955 432 747 268 1494
Fragments arranged by size:
1494 955 747 432 387 268 15
EcoNI CCTnn'n_nnAGG
Cuts at: 1060 1060
Size: 4298
EcoO109I rG'GnC_Cy
Cuts at: 284 502 503 708 1437 284
Size: 218 1 205 729 3145
Fragments arranged by size:
3145 729 218 205 1
EcoRI G'AATT_C
Cuts at: 1302 1302
Size: 4298
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2317 2317
Size: 4298
FspI TGC'GCA
Cuts at: 2079 2079
Size: 4298
HaeII r_GCGC'y
Cuts at: 249 294 1732 1980 3002 3981 249
Size: 45 1438 248 1022 979 566
Fragments arranged by size:
1438 1022 979 566 248 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2140 2170 2172 2202 2140
Size: 30 2 30 4236
Fragments arranged by size:
4236 30 30 2
HincII GTy'rAC
Cuts at: 1337 1337
Size: 4298
KasI G'GCGC_C
Cuts at: 290 1728 1976 290
Size: 1438 248 2612
Fragments arranged by size:
2612 1438 248
KpnI G_GTAC'C
Cuts at: 693 1318 693
Size: 625 3673
MluI A'CGCG_T
Cuts at: 3731 3731
Size: 4298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2969 3153 2969
Size: 184 4114
MscI TGG'CCA
Cuts at: 163 2059 163
Size: 1896 2402
MslI CAynn'nnrTG
Cuts at: 616 640 892 950 2287 2414 616
Size: 24 252 58 1337 127 2500
Fragments arranged by size:
2500 1337 252 127 58 24
MspA1I CmG'CkG
Cuts at: 127 359 510 1269 1409 1723 2083 3096 3341
Size: 232 151 759 140 314 360 1013 245
Cuts at: 3341 127
Size: 1084
Fragments arranged by size:
1084 1013 759 360 314 245 232 151
140
NaeI GCC'GGC
Cuts at: 535 2479 535
Size: 1944 2354
NarI GG'CG_CC
Cuts at: 291 1729 1977 291
Size: 1438 248 2612
Fragments arranged by size:
2612 1438 248
NcoI C'CATG_G
Cuts at: 107 680 107
Size: 573 3725
NgoMIV G'CCGG_C
Cuts at: 533 2477 533
Size: 1944 2354
NspI r_CATG'y
Cuts at: 1351 1851 2758 1351
Size: 500 907 2891
Fragments arranged by size:
2891 907 500
PciI A'CATG_T
Cuts at: 2754 2754
Size: 4298
PfoI T'CCnGG_A
Cuts at: 368 704 836 368
Size: 336 132 3830
Fragments arranged by size:
3830 336 132
PmeI GTTT'AAAC
Cuts at: 1297 1297
Size: 4298
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3437 3469 3437
Size: 32 4266
PpuMI rG'GwC_Cy
Cuts at: 708 708
Size: 4298
PshAI GACnn'nnGTC
Cuts at: 1276 1276
Size: 4298
PsiI TTA'TAA
Cuts at: 917 917
Size: 4298
PspOMI G'GGCC_C
Cuts at: 502 502
Size: 4298
PstI C_TGCA'G
Cuts at: 1345 2030 1345
Size: 685 3613
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4298
PvuII CAG'CTG
Cuts at: 127 510 1269 1723 2083 127
Size: 383 759 454 360 2342
Fragments arranged by size:
2342 759 454 383 360
RsrII CG'GwC_CG
Cuts at: 2493 2493
Size: 4298
SacI G_AGCT'C
Cuts at: 1312 1312
Size: 4298
SalI G'TCGA_C
Cuts at: 1335 1335
Size: 4298
SapI GCTCTTCn'nnn_
Cuts at: 358 2321 2531 358
Size: 1963 210 2125
Fragments arranged by size:
2125 1963 210
SbfI CC_TGCA'GG
Cuts at: 1345 1345
Size: 4298
ScaI AGT'ACT
Cuts at: 274 3815 274
Size: 3541 757
SfcI C'TryA_G
Cuts at: 33 921 1341 2026 3019 3210 33
Size: 888 420 685 993 191 1121
Fragments arranged by size:
1121 993 888 685 420 191
SfoI GGC'GCC
Cuts at: 292 1730 1978 292
Size: 1438 248 2612
Fragments arranged by size:
2612 1438 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4298
SmaI CCC'GGG
Cuts at: 403 1320 403
Size: 917 3381
SmlI C'TyrA_G
Cuts at: 572 1022 1453 2368 2860 3122 572
Size: 450 431 915 492 262 1748
Fragments arranged by size:
1748 915 492 450 431 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4298
SphI G_CATG'C
Cuts at: 1351 1851 1351
Size: 500 3798
StuI AGG'CCT
Cuts at: 338 338
Size: 4298
StyI C'CwwG_G
Cuts at: 107 653 680 1432 1786 107
Size: 546 27 752 354 2619
Fragments arranged by size:
2619 752 546 354 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1881 2014 2473 2682 1881
Size: 133 459 209 3497
Fragments arranged by size:
3497 459 209 133
TatI w'GTAC_w
Cuts at: 272 545 752 905 3813 272
Size: 273 207 153 2908 757
Fragments arranged by size:
2908 757 273 207 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1273 1305 3815 3847 1273
Size: 32 2510 32 1724
Fragments arranged by size:
2510 1724 32 32
Tth111I GACn'n_nGTC
Cuts at: 2095 2095
Size: 4298
XbaI T'CTAG_A
Cuts at: 1329 1329
Size: 4298
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 670 1793 670
Size: 1123 3175
XmaI C'CCGG_G
Cuts at: 401 1318 401
Size: 917 3381
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AvrII BaeI BfrBI
BmgBI BmtI BsaXI BsiWI BsmI BsmBI BstEII BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI