Restriction map for
pF1KE0492
(Circular) (Six-base) MAPSORT of: pF1KE0492.seq Check: 1030 from: 1 to: 3953
pF1KE0492 3953 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 991 991
Size: 3953
Acc65I G'GTAC_C
Cuts at: 969 969
Size: 3953
AclI AA'CG_TT
Cuts at: 3793 3793
Size: 3953
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 1778 2210 2957 3225 406
Size: 1372 432 747 268 1134
Fragments arranged by size:
1372 1134 747 432 268
AfeI AGC'GCT
Cuts at: 279 279
Size: 3953
AflIII A'CryG_T
Cuts at: 1305 2409 3386 1305
Size: 1104 977 1872
Fragments arranged by size:
1872 1104 977
AgeI A'CCGG_T
Cuts at: 2341 2341
Size: 3953
AhdI GACnn_n'nnGTC
Cuts at: 372 372
Size: 3953
AlwNI CAG_nnn'CTG
Cuts at: 243 261 1258 2825 243
Size: 18 997 1567 1371
Fragments arranged by size:
1567 1371 997 18
ApaI G_GGCC'C
Cuts at: 153 153
Size: 3953
ApaLI G'TGCA_C
Cuts at: 2723 2723
Size: 3953
ApoI r'AATT_y
Cuts at: 957 3306 3562 957
Size: 2349 256 1348
Fragments arranged by size:
2349 1348 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3953
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3953
AvaI C'yCGr_G
Cuts at: 411 432 973 411
Size: 21 541 3391
Fragments arranged by size:
3391 541 21
AvrII C'CTAG_G
Cuts at: 294 294
Size: 3953
BamHI G'GATC_C
Cuts at: 978 978
Size: 3953
BanI G'GyrC_C
Cuts at: 860 969 1383 1631 1666 860
Size: 109 414 248 35 3147
Fragments arranged by size:
3147 414 248 109 35
BanII G_rGCy'C
Cuts at: 153 400 447 489 940 967 1997 153
Size: 247 47 42 451 27 1030 2109
Fragments arranged by size:
2109 1030 451 247 47 42 27
BbeI G_GCGC'C
Cuts at: 1387 1635 1387
Size: 248 3705
BbsI GAAGACnn'nnnn_
Cuts at: 612 681 844 612
Size: 69 163 3721
Fragments arranged by size:
3721 163 69
BbvCI CC'TCA_GC
Cuts at: 749 749
Size: 3953
BciVI GTATCCnnnnn_n'
Cuts at: 1851 2612 3953 1851
Size: 761 1341 1851
Fragments arranged by size:
1851 1341 761
BclI T'GATC_A
Cuts at: 1473 1473
Size: 3953
BglII A'GATC_T
Cuts at: 1468 3505 1468
Size: 2037 1916
BlpI GC'TnA_GC
Cuts at: 446 1065 446
Size: 619 3334
Bme1580I G_kGCm'C
Cuts at: 153 1578 1671 2727 153
Size: 1425 93 1056 1379
Fragments arranged by size:
1425 1379 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1165 1246 1581 3725 1165
Size: 81 335 2144 1393
Fragments arranged by size:
2144 1393 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2320 2320
Size: 3953
Bpu10I CC'TnA_GC
Cuts at: 488 749 488
Size: 261 3692
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 1129 2008 2500 2798 266
Size: 863 879 492 298 1421
Fragments arranged by size:
1421 879 863 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 588 3657 588
Size: 3069 884
BsaAI yAC'GTr
Cuts at: 1306 1306
Size: 3953
BsaBI GATnn'nnATC
Cuts at: 6 1467 3504 6
Size: 1461 2037 455
Fragments arranged by size:
2037 1461 455
BsaHI Gr'CG_yC
Cuts at: 1384 1632 2334 3830 1384
Size: 248 702 1496 1507
Fragments arranged by size:
1507 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1142 1663 2341 2615 2762 3173 1142
Size: 521 678 274 147 411 1922
Fragments arranged by size:
1922 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 399 513 604 672 766 399
Size: 114 91 68 94 3586
Fragments arranged by size:
3586 114 94 91 68
BseYI C'CCAG_C
Cuts at: 114 475 652 1378 2136 2713 3209 114
Size: 361 177 726 758 577 496 858
Fragments arranged by size:
858 758 726 577 496 361 177
BsiEI CG_ry'CG
Cuts at: 104 1495 1541 2749 104
Size: 1391 46 1208 1308
Fragments arranged by size:
1391 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 505 940 967 1745 1935 2727 505
Size: 435 27 778 190 792 1731
Fragments arranged by size:
1731 792 778 435 190 27
BsmI GAATG_Cn'
Cuts at: 396 396
Size: 3953
Bsp1286I G_dGCh'C
Cuts at: 153 400 447 489 505 940 967 1578 1671
Size: 247 47 42 16 435 27 611 93
Cuts at: 1671 1745 1935 1997 2727 153
Size: 74 190 62 730 1379
Fragments arranged by size:
1379 730 611 435 247 190 93 74
62 47 42 27 16
BspEI T'CCGG_A
Cuts at: 3173 3173
Size: 3953
BspHI T'CATG_A
Cuts at: 767 3129 767
Size: 2362 1591
BspMI ACCTGCnnnn'nnnn_
Cuts at: 118 1003 1217 1519 1900 118
Size: 885 214 302 381 2171
Fragments arranged by size:
2171 885 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2245 2299 3783 3948 50
Size: 2195 54 1484 165 55
Fragments arranged by size:
2195 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 570 1865 570
Size: 1295 2658
BsrFI r'CCGG_y
Cuts at: 1951 2132 2341 1951
Size: 181 209 3563
Fragments arranged by size:
3563 209 181
BssSI C'ACGA_G
Cuts at: 2224 2582 2224
Size: 358 3595
BstAPI GCAn_nnn'nTGC
Cuts at: 1330 1330
Size: 3953
BstBI TT'CG_AA
Cuts at: 2314 2314
Size: 3953
BstXI CCAn_nnnn'nTGG
Cuts at: 114 556 114
Size: 442 3511
BstYI r'GATC_y
Cuts at: 1 978 1445 1468 1803 2049 3061 3147 3159
Size: 977 467 23 335 246 1012 86 12
Cuts at: 3159 3505 1
Size: 346 449
Fragments arranged by size:
1012 977 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 120 107
Size: 13 3940
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1903 2084 2247 1903
Size: 181 163 3609
Fragments arranged by size:
3609 181 163
BtsI GCAGTG_nn'
Cuts at: 307 1196 307
Size: 889 3064
DraI TTT'AAA
Cuts at: 952 952
Size: 3953
DrdI GACnn_nn'nnGTC
Cuts at: 580 1659 2517 580
Size: 1079 858 2016
Fragments arranged by size:
2016 1079 858
EaeI y'GGCC_r
Cuts at: 1538 1712 2103 2130 3475 1538
Size: 174 391 27 1345 2016
Fragments arranged by size:
2016 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1538 1538
Size: 3953
EarI CTCTTCn'nnn_
Cuts at: 899 1976 2186 899
Size: 1077 210 2666
Fragments arranged by size:
2666 1077 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2161 2471 2617 3763 2161
Size: 310 146 1146 2351
Fragments arranged by size:
2351 1146 310 146
EcoICRI GAG'CTC
Cuts at: 938 965 938
Size: 27 3926
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 1778 2210 2957 3225 406
Size: 1372 432 747 268 1134
Fragments arranged by size:
1372 1134 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 769 769
Size: 3953
EcoO109I rG'GnC_Cy
Cuts at: 149 380 659 1092 149
Size: 231 279 433 3010
Fragments arranged by size:
3010 433 279 231
EcoRI G'AATT_C
Cuts at: 957 957
Size: 3953
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1972 1972
Size: 3953
FspI TGC'GCA
Cuts at: 1734 1734
Size: 3953
HaeII r_GCGC'y
Cuts at: 281 1387 1635 2657 3636 281
Size: 1106 248 1022 979 598
Fragments arranged by size:
1106 1022 979 598 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 309 341 358 390 651 683 831 863 1795
Size: 32 17 32 261 32 148 32 932
Cuts at: 1795 1825 1827 1857 309
Size: 30 2 30 2405
Fragments arranged by size:
2405 932 261 148 32 32 32 32
30 30 17 2
HincII GTy'rAC
Cuts at: 992 992
Size: 3953
KasI G'GCGC_C
Cuts at: 1383 1631 1383
Size: 248 3705
KpnI G_GTAC'C
Cuts at: 973 973
Size: 3953
MluI A'CGCG_T
Cuts at: 3386 3386
Size: 3953
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2624 2808 2624
Size: 184 3769
MscI TGG'CCA
Cuts at: 1714 1714
Size: 3953
MslI CAynn'nnrTG
Cuts at: 158 827 1942 2069 158
Size: 669 1115 127 2042
Fragments arranged by size:
2042 1115 669 127
MspA1I CmG'CkG
Cuts at: 579 1064 1378 1738 2751 2996 579
Size: 485 314 360 1013 245 1536
Fragments arranged by size:
1536 1013 485 360 314 245
NaeI GCC'GGC
Cuts at: 2134 2134
Size: 3953
NarI GG'CG_CC
Cuts at: 1384 1632 1384
Size: 248 3705
NcoI C'CATG_G
Cuts at: 107 107
Size: 3953
NgoMIV G'CCGG_C
Cuts at: 2132 2132
Size: 3953
NspI r_CATG'y
Cuts at: 723 1006 1506 2413 723
Size: 283 500 907 2263
Fragments arranged by size:
2263 907 500 283
PasI CC'CwG_GG
Cuts at: 267 267
Size: 3953
PciI A'CATG_T
Cuts at: 2409 2409
Size: 3953
PmeI GTTT'AAAC
Cuts at: 952 952
Size: 3953
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3092 3124 3092
Size: 32 3921
PpuMI rG'GwC_Cy
Cuts at: 380 659 380
Size: 279 3674
PspOMI G'GGCC_C
Cuts at: 149 149
Size: 3953
PstI C_TGCA'G
Cuts at: 1000 1685 1000
Size: 685 3268
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3953
PvuII CAG'CTG
Cuts at: 579 1378 1738 579
Size: 799 360 2794
Fragments arranged by size:
2794 799 360
RsrII CG'GwC_CG
Cuts at: 2148 2148
Size: 3953
SacI G_AGCT'C
Cuts at: 940 967 940
Size: 27 3926
SalI G'TCGA_C
Cuts at: 990 990
Size: 3953
SapI GCTCTTCn'nnn_
Cuts at: 1976 2186 1976
Size: 210 3743
SbfI CC_TGCA'GG
Cuts at: 1000 1000
Size: 3953
ScaI AGT'ACT
Cuts at: 3470 3470
Size: 3953
SfcI C'TryA_G
Cuts at: 33 204 996 1681 2674 2865 33
Size: 171 792 685 993 191 1121
Fragments arranged by size:
1121 993 792 685 191 171
SfoI GGC'GCC
Cuts at: 1385 1633 1385
Size: 248 3705
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3953
SmaI CCC'GGG
Cuts at: 975 975
Size: 3953
SmlI C'TyrA_G
Cuts at: 281 411 1108 2023 2515 2777 281
Size: 130 697 915 492 262 1457
Fragments arranged by size:
1457 915 697 492 262 130
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3953
SphI G_CATG'C
Cuts at: 1006 1506 1006
Size: 500 3453
StyI C'CwwG_G
Cuts at: 107 212 294 1087 1441 107
Size: 105 82 793 354 2619
Fragments arranged by size:
2619 793 354 105 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1536 1669 2128 2337 1536
Size: 133 459 209 3152
Fragments arranged by size:
3152 459 209 133
TatI w'GTAC_w
Cuts at: 3468 3468
Size: 3953
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3470 3502 3470
Size: 32 3921
Tth111I GACn'n_nGTC
Cuts at: 1750 1750
Size: 3953
XbaI T'CTAG_A
Cuts at: 984 984
Size: 3953
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1448 1448
Size: 3953
XhoI C'TCGA_G
Cuts at: 411 411
Size: 3953
XmaI C'CCGG_G
Cuts at: 973 973
Size: 3953
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI AloI AscI BaeI
BcgI BfrBI BglI BmgBI BmtI BpmI BsaXI BsgI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PfoI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XmnI
ZraI