Restriction map for
pF1KE0489
(Circular) (Six-base) MAPSORT of: pF1KE0489.seq Check: 2031 from: 1 to: 3620
pF1KE0489 3620 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 658 658
Size: 3620
Acc65I G'GTAC_C
Cuts at: 485 636 485
Size: 151 3469
AclI AA'CG_TT
Cuts at: 3460 3460
Size: 3620
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1445 1877 2624 2892 1445
Size: 432 747 268 2173
Fragments arranged by size:
2173 747 432 268
AflIII A'CryG_T
Cuts at: 972 2076 3053 972
Size: 1104 977 1539
Fragments arranged by size:
1539 1104 977
AgeI A'CCGG_T
Cuts at: 2008 2008
Size: 3620
AleI CACnn'nnGTG
Cuts at: 475 475
Size: 3620
AlwNI CAG_nnn'CTG
Cuts at: 925 2492 925
Size: 1567 2053
ApaI G_GGCC'C
Cuts at: 259 259
Size: 3620
ApaLI G'TGCA_C
Cuts at: 2390 2390
Size: 3620
ApoI r'AATT_y
Cuts at: 624 2973 3229 624
Size: 2349 256 1015
Fragments arranged by size:
2349 1015 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3620
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3620
AvaI C'yCGr_G
Cuts at: 640 640
Size: 3620
BamHI G'GATC_C
Cuts at: 645 645
Size: 3620
BanI G'GyrC_C
Cuts at: 123 147 233 409 485 636 1050 1298 1333
Size: 24 86 176 76 151 414 248 35
Cuts at: 1333 123
Size: 2410
Fragments arranged by size:
2410 414 248 176 151 86 76 35
24
BanII G_rGCy'C
Cuts at: 175 259 499 634 1664 175
Size: 84 240 135 1030 2131
Fragments arranged by size:
2131 1030 240 135 84
BbeI G_GCGC'C
Cuts at: 151 1054 1302 151
Size: 903 248 2469
Fragments arranged by size:
2469 903 248
BciVI GTATCCnnnnn_n'
Cuts at: 1518 2279 3620 1518
Size: 761 1341 1518
Fragments arranged by size:
1518 1341 761
BclI T'GATC_A
Cuts at: 1140 1140
Size: 3620
BglI GCCn_nnn'nGGC
Cuts at: 231 538 231
Size: 307 3313
BglII A'GATC_T
Cuts at: 1135 3172 1135
Size: 2037 1583
BlpI GC'TnA_GC
Cuts at: 732 732
Size: 3620
Bme1580I G_kGCm'C
Cuts at: 128 259 271 414 1245 1338 2394 128
Size: 131 12 143 831 93 1056 1354
Fragments arranged by size:
1354 1056 831 143 131 93 12
BmrI ACTGGGnnnn_n'
Cuts at: 255 374 393 832 913 1248 3392 255
Size: 119 19 439 81 335 2144 483
Fragments arranged by size:
2144 483 439 335 119 81 19
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1987 1987
Size: 3620
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 796 1675 2167 2465 796
Size: 879 492 298 1951
Fragments arranged by size:
1951 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 202 3324 202
Size: 3122 498
BsaAI yAC'GTr
Cuts at: 973 973
Size: 3620
BsaBI GATnn'nnATC
Cuts at: 6 1134 3171 6
Size: 1128 2037 455
Fragments arranged by size:
2037 1128 455
BsaHI Gr'CG_yC
Cuts at: 148 1051 1299 2001 3497 148
Size: 903 248 702 1496 271
Fragments arranged by size:
1496 903 702 271 248
BsaWI w'CCGG_w
Cuts at: 809 1330 2008 2282 2429 2840 809
Size: 521 678 274 147 411 1589
Fragments arranged by size:
1589 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 151 252 318 425 1045 1803 2380 2876 151
Size: 101 66 107 620 758 577 496 895
Fragments arranged by size:
895 758 620 577 496 107 101 66
BsiEI CG_ry'CG
Cuts at: 104 1162 1208 2416 104
Size: 1058 46 1208 1308
Fragments arranged by size:
1308 1208 1058 46
BsiHKAI G_wGCw'C
Cuts at: 634 1412 1602 2394 634
Size: 778 190 792 1860
Fragments arranged by size:
1860 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 128 175 259 271 414 499 634 1245 1338
Size: 47 84 12 143 85 135 611 93
Cuts at: 1338 1412 1602 1664 2394 128
Size: 74 190 62 730 1354
Fragments arranged by size:
1354 730 611 190 143 135 93 85
84 74 62 47 12
BspEI T'CCGG_A
Cuts at: 2840 2840
Size: 3620
BspHI T'CATG_A
Cuts at: 2796 2796
Size: 3620
BspMI ACCTGCnnnn'nnnn_
Cuts at: 670 884 1186 1567 670
Size: 214 302 381 2723
Fragments arranged by size:
2723 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1912 1966 3450 3615 50
Size: 1862 54 1484 165 55
Fragments arranged by size:
1862 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1532 1532
Size: 3620
BsrFI r'CCGG_y
Cuts at: 211 1618 1799 2008 211
Size: 1407 181 209 1823
Fragments arranged by size:
1823 1407 209 181
BssSI C'ACGA_G
Cuts at: 228 1891 2249 228
Size: 1663 358 1599
Fragments arranged by size:
1663 1599 358
BstAPI GCAn_nnn'nTGC
Cuts at: 997 997
Size: 3620
BstBI TT'CG_AA
Cuts at: 1981 1981
Size: 3620
BstXI CCAn_nnnn'nTGG
Cuts at: 477 477
Size: 3620
BstYI r'GATC_y
Cuts at: 1 438 645 1112 1135 1470 1716 2728 2814
Size: 437 207 467 23 335 246 1012 86
Cuts at: 2814 2826 3172 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 437 346 335 246 207
86 23 12
BtgI C'CryG_G
Cuts at: 107 131 186 284 476 582 107
Size: 24 55 98 192 106 3145
Fragments arranged by size:
3145 192 106 98 55 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1570 1751 1914 1570
Size: 181 163 3276
Fragments arranged by size:
3276 181 163
BtsI GCAGTG_nn'
Cuts at: 863 863
Size: 3620
DraI TTT'AAA
Cuts at: 619 619
Size: 3620
DraIII CAC_nnn'GTG
Cuts at: 249 249
Size: 3620
DrdI GACnn_nn'nnGTC
Cuts at: 1326 2184 1326
Size: 858 2762
EaeI y'GGCC_r
Cuts at: 503 1205 1379 1770 1797 3142 503
Size: 702 174 391 27 1345 981
Fragments arranged by size:
1345 981 702 391 174 27
EagI C'GGCC_G
Cuts at: 1205 1205
Size: 3620
EarI CTCTTCn'nnn_
Cuts at: 1643 1853 1643
Size: 210 3410
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1828 2138 2284 3430 1828
Size: 310 146 1146 2018
Fragments arranged by size:
2018 1146 310 146
EcoICRI GAG'CTC
Cuts at: 632 632
Size: 3620
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1445 1877 2624 2892 1445
Size: 432 747 268 2173
Fragments arranged by size:
2173 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 256 759 256
Size: 503 3117
EcoRI G'AATT_C
Cuts at: 624 624
Size: 3620
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1639 1639
Size: 3620
FspI TGC'GCA
Cuts at: 1401 1401
Size: 3620
HaeII r_GCGC'y
Cuts at: 151 1054 1302 2324 3303 151
Size: 903 248 1022 979 468
Fragments arranged by size:
1022 979 903 468 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1462 1492 1494 1524 1462
Size: 30 2 30 3558
Fragments arranged by size:
3558 30 30 2
HincII GTy'rAC
Cuts at: 659 659
Size: 3620
KasI G'GCGC_C
Cuts at: 147 1050 1298 147
Size: 903 248 2469
Fragments arranged by size:
2469 903 248
KpnI G_GTAC'C
Cuts at: 489 640 489
Size: 151 3469
MluI A'CGCG_T
Cuts at: 3053 3053
Size: 3620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2291 2475 2291
Size: 184 3436
MscI TGG'CCA
Cuts at: 1381 1381
Size: 3620
MslI CAynn'nnrTG
Cuts at: 475 1609 1736 475
Size: 1134 127 2359
Fragments arranged by size:
2359 1134 127
MspA1I CmG'CkG
Cuts at: 203 372 501 731 1045 1405 2418 2663 203
Size: 169 129 230 314 360 1013 245 1160
Fragments arranged by size:
1160 1013 360 314 245 230 169 129
NaeI GCC'GGC
Cuts at: 213 1801 213
Size: 1588 2032
NarI GG'CG_CC
Cuts at: 148 1051 1299 148
Size: 903 248 2469
Fragments arranged by size:
2469 903 248
NcoI C'CATG_G
Cuts at: 107 131 284 582 107
Size: 24 153 298 3145
Fragments arranged by size:
3145 298 153 24
NgoMIV G'CCGG_C
Cuts at: 211 1799 211
Size: 1588 2032
NspI r_CATG'y
Cuts at: 673 1173 2080 673
Size: 500 907 2213
Fragments arranged by size:
2213 907 500
PasI CC'CwG_GG
Cuts at: 119 351 119
Size: 232 3388
PciI A'CATG_T
Cuts at: 2076 2076
Size: 3620
PmeI GTTT'AAAC
Cuts at: 619 619
Size: 3620
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2759 2791 2759
Size: 32 3588
PspOMI G'GGCC_C
Cuts at: 255 255
Size: 3620
PstI C_TGCA'G
Cuts at: 221 667 1352 221
Size: 446 685 2489
Fragments arranged by size:
2489 685 446
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3620
PvuII CAG'CTG
Cuts at: 372 1045 1405 372
Size: 673 360 2587
Fragments arranged by size:
2587 673 360
RsrII CG'GwC_CG
Cuts at: 1815 1815
Size: 3620
SacI G_AGCT'C
Cuts at: 634 634
Size: 3620
SalI G'TCGA_C
Cuts at: 657 657
Size: 3620
SapI GCTCTTCn'nnn_
Cuts at: 1643 1853 1643
Size: 210 3410
SbfI CC_TGCA'GG
Cuts at: 667 667
Size: 3620
ScaI AGT'ACT
Cuts at: 3137 3137
Size: 3620
SexAI A'CCwGG_T
Cuts at: 302 488 302
Size: 186 3434
SfcI C'TryA_G
Cuts at: 33 217 663 1348 2341 2532 33
Size: 184 446 685 993 191 1121
Fragments arranged by size:
1121 993 685 446 191 184
SfiI GGCCn_nnn'nGGCC
Cuts at: 538 538
Size: 3620
SfoI GGC'GCC
Cuts at: 149 1052 1300 149
Size: 903 248 2469
Fragments arranged by size:
2469 903 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3620
SmaI CCC'GGG
Cuts at: 642 642
Size: 3620
SmlI C'TyrA_G
Cuts at: 775 1690 2182 2444 775
Size: 915 492 262 1951
Fragments arranged by size:
1951 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3620
SphI G_CATG'C
Cuts at: 673 1173 673
Size: 500 3120
StyI C'CwwG_G
Cuts at: 107 131 284 431 582 754 1108 107
Size: 24 153 147 151 172 354 2619
Fragments arranged by size:
2619 354 172 153 151 147 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 111 237 1203 1336 1795 2004 111
Size: 126 966 133 459 209 1727
Fragments arranged by size:
1727 966 459 209 133 126
TatI w'GTAC_w
Cuts at: 3135 3135
Size: 3620
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3137 3169 3137
Size: 32 3588
Tth111I GACn'n_nGTC
Cuts at: 1417 1417
Size: 3620
XbaI T'CTAG_A
Cuts at: 651 651
Size: 3620
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 589 1115 589
Size: 526 3094
XmaI C'CCGG_G
Cuts at: 640 640
Size: 3620
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PfoI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI