Restriction map for
pF1KE0486
(Circular) (Six-base) MAPSORT of: pF1KE0486.seq Check: 9486 from: 1 to: 3653
pF1KE0486 3653 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 691 691
Size: 3653
Acc65I G'GTAC_C
Cuts at: 669 669
Size: 3653
AclI AA'CG_TT
Cuts at: 3493 3493
Size: 3653
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 1478 1910 2657 2925 259
Size: 1219 432 747 268 987
Fragments arranged by size:
1219 987 747 432 268
AflIII A'CryG_T
Cuts at: 1005 2109 3086 1005
Size: 1104 977 1572
Fragments arranged by size:
1572 1104 977
AgeI A'CCGG_T
Cuts at: 2041 2041
Size: 3653
AleI CACnn'nnGTG
Cuts at: 457 457
Size: 3653
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 581 581
Size: 3653
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 589 621 589
Size: 32 3621
AlwNI CAG_nnn'CTG
Cuts at: 198 418 543 958 2525 198
Size: 220 125 415 1567 1326
Fragments arranged by size:
1567 1326 415 220 125
ApaI G_GGCC'C
Cuts at: 631 631
Size: 3653
ApaLI G'TGCA_C
Cuts at: 2423 2423
Size: 3653
ApoI r'AATT_y
Cuts at: 657 3006 3262 657
Size: 2349 256 1048
Fragments arranged by size:
2349 1048 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3653
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3653
AvaI C'yCGr_G
Cuts at: 673 673
Size: 3653
BamHI G'GATC_C
Cuts at: 678 678
Size: 3653
BanI G'GyrC_C
Cuts at: 669 1083 1331 1366 669
Size: 414 248 35 2956
Fragments arranged by size:
2956 414 248 35
BanII G_rGCy'C
Cuts at: 631 667 1697 631
Size: 36 1030 2587
Fragments arranged by size:
2587 1030 36
BbeI G_GCGC'C
Cuts at: 1087 1335 1087
Size: 248 3405
BciVI GTATCCnnnnn_n'
Cuts at: 1551 2312 3653 1551
Size: 761 1341 1551
Fragments arranged by size:
1551 1341 761
BclI T'GATC_A
Cuts at: 1173 1173
Size: 3653
BglI GCCn_nnn'nGGC
Cuts at: 532 532
Size: 3653
BglII A'GATC_T
Cuts at: 560 1168 3205 560
Size: 608 2037 1008
Fragments arranged by size:
2037 1008 608
BlpI GC'TnA_GC
Cuts at: 765 765
Size: 3653
Bme1580I G_kGCm'C
Cuts at: 175 581 631 1278 1371 2427 175
Size: 406 50 647 93 1056 1401
Fragments arranged by size:
1401 1056 647 406 93 50
BmrI ACTGGGnnnn_n'
Cuts at: 865 946 1281 3425 865
Size: 81 335 2144 1093
Fragments arranged by size:
2144 1093 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 648 2020 648
Size: 1372 2281
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 258 325 526 658 258
Size: 67 201 132 3253
Fragments arranged by size:
3253 201 132 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 829 1708 2200 2498 829
Size: 879 492 298 1984
Fragments arranged by size:
1984 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3357 3357
Size: 3653
BsaAI yAC'GTr
Cuts at: 576 1006 576
Size: 430 3223
BsaBI GATnn'nnATC
Cuts at: 6 1167 3204 6
Size: 1161 2037 455
Fragments arranged by size:
2037 1161 455
BsaHI Gr'CG_yC
Cuts at: 1084 1332 2034 3530 1084
Size: 248 702 1496 1207
Fragments arranged by size:
1496 1207 702 248
BsaWI w'CCGG_w
Cuts at: 842 1363 2041 2315 2462 2873 842
Size: 521 678 274 147 411 1622
Fragments arranged by size:
1622 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 214 244 214
Size: 30 3623
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 367 367
Size: 3653
BseYI C'CCAG_C
Cuts at: 418 1078 1836 2413 2909 418
Size: 660 758 577 496 1162
Fragments arranged by size:
1162 758 660 577 496
BsiEI CG_ry'CG
Cuts at: 104 1195 1241 2449 104
Size: 1091 46 1208 1308
Fragments arranged by size:
1308 1208 1091 46
BsiHKAI G_wGCw'C
Cuts at: 667 1445 1635 2427 667
Size: 778 190 792 1893
Fragments arranged by size:
1893 792 778 190
BsmI GAATG_Cn'
Cuts at: 440 440
Size: 3653
Bsp1286I G_dGCh'C
Cuts at: 175 581 631 667 1278 1371 1445 1635 1697
Size: 406 50 36 611 93 74 190 62
Cuts at: 1697 2427 175
Size: 730 1401
Fragments arranged by size:
1401 730 611 406 190 93 74 62
50 36
BspEI T'CCGG_A
Cuts at: 2873 2873
Size: 3653
BspHI T'CATG_A
Cuts at: 2829 2829
Size: 3653
BspMI ACCTGCnnnn'nnnn_
Cuts at: 608 703 917 1219 1600 608
Size: 95 214 302 381 2661
Fragments arranged by size:
2661 381 302 214 95
BsrBI CCG'CTC
Cuts at: 50 1945 1999 3483 3648 50
Size: 1895 54 1484 165 55
Fragments arranged by size:
1895 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1565 1565
Size: 3653
BsrFI r'CCGG_y
Cuts at: 446 1651 1832 2041 446
Size: 1205 181 209 2058
Fragments arranged by size:
2058 1205 209 181
BssSI C'ACGA_G
Cuts at: 1924 2282 1924
Size: 358 3295
BstAPI GCAn_nnn'nTGC
Cuts at: 1030 1030
Size: 3653
BstBI TT'CG_AA
Cuts at: 2014 2014
Size: 3653
BstXI CCAn_nnnn'nTGG
Cuts at: 165 165
Size: 3653
BstYI r'GATC_y
Cuts at: 1 560 678 1145 1168 1503 1749 2761 2847
Size: 559 118 467 23 335 246 1012 86
Cuts at: 2847 2859 3205 1
Size: 12 346 449
Fragments arranged by size:
1012 559 467 449 346 335 246 118
86 23 12
BtgI C'CryG_G
Cuts at: 455 530 455
Size: 75 3578
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1603 1784 1947 1603
Size: 181 163 3309
Fragments arranged by size:
3309 181 163
BtsI GCAGTG_nn'
Cuts at: 432 896 432
Size: 464 3189
DraI TTT'AAA
Cuts at: 652 652
Size: 3653
DraIII CAC_nnn'GTG
Cuts at: 456 456
Size: 3653
DrdI GACnn_nn'nnGTC
Cuts at: 1359 2217 1359
Size: 858 2795
EaeI y'GGCC_r
Cuts at: 152 570 1238 1412 1803 1830 3175 152
Size: 418 668 174 391 27 1345 630
Fragments arranged by size:
1345 668 630 418 391 174 27
EagI C'GGCC_G
Cuts at: 1238 1238
Size: 3653
EarI CTCTTCn'nnn_
Cuts at: 191 1676 1886 191
Size: 1485 210 1958
Fragments arranged by size:
1958 1485 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 217 1861 2171 2317 3463 217
Size: 1644 310 146 1146 407
Fragments arranged by size:
1644 1146 407 310 146
EcoICRI GAG'CTC
Cuts at: 665 665
Size: 3653
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 258 259 325 526 658 1478 1910 2657 2925
Size: 1 66 201 132 820 432 747 268
Cuts at: 2925 258
Size: 986
Fragments arranged by size:
986 820 747 432 268 201 132 66
1
EcoO109I rG'GnC_Cy
Cuts at: 122 627 628 642 792 122
Size: 505 1 14 150 2983
Fragments arranged by size:
2983 505 150 14 1
EcoRI G'AATT_C
Cuts at: 657 657
Size: 3653
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 264 1672 264
Size: 1408 2245
FspI TGC'GCA
Cuts at: 1434 1434
Size: 3653
HaeII r_GCGC'y
Cuts at: 543 1087 1335 2357 3336 543
Size: 544 248 1022 979 860
Fragments arranged by size:
1022 979 860 544 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 214 246 1495 1525 1527 1557 214
Size: 32 1249 30 2 30 2310
Fragments arranged by size:
2310 1249 32 30 30 2
HincII GTy'rAC
Cuts at: 692 692
Size: 3653
KasI G'GCGC_C
Cuts at: 1083 1331 1083
Size: 248 3405
KpnI G_GTAC'C
Cuts at: 673 673
Size: 3653
MluI A'CGCG_T
Cuts at: 3086 3086
Size: 3653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2324 2508 2324
Size: 184 3469
MscI TGG'CCA
Cuts at: 572 1414 572
Size: 842 2811
MslI CAynn'nnrTG
Cuts at: 112 371 457 1642 1769 112
Size: 259 86 1185 127 1996
Fragments arranged by size:
1996 1185 259 127 86
MspA1I CmG'CkG
Cuts at: 198 764 1078 1438 2451 2696 198
Size: 566 314 360 1013 245 1155
Fragments arranged by size:
1155 1013 566 360 314 245
NaeI GCC'GGC
Cuts at: 1834 1834
Size: 3653
NarI GG'CG_CC
Cuts at: 1084 1332 1084
Size: 248 3405
NcoI C'CATG_G
Cuts at: 530 530
Size: 3653
NgoMIV G'CCGG_C
Cuts at: 1832 1832
Size: 3653
NspI r_CATG'y
Cuts at: 706 1206 2113 706
Size: 500 907 2246
Fragments arranged by size:
2246 907 500
PciI A'CATG_T
Cuts at: 2109 2109
Size: 3653
PmeI GTTT'AAAC
Cuts at: 652 652
Size: 3653
PmlI CAC'GTG
Cuts at: 576 576
Size: 3653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2792 2824 2792
Size: 32 3621
PpuMI rG'GwC_Cy
Cuts at: 642 642
Size: 3653
PspOMI G'GGCC_C
Cuts at: 627 627
Size: 3653
PstI C_TGCA'G
Cuts at: 194 439 560 700 1385 194
Size: 245 121 140 685 2462
Fragments arranged by size:
2462 685 245 140 121
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3653
PvuII CAG'CTG
Cuts at: 198 1078 1438 198
Size: 880 360 2413
Fragments arranged by size:
2413 880 360
RsrII CG'GwC_CG
Cuts at: 1848 1848
Size: 3653
SacI G_AGCT'C
Cuts at: 667 667
Size: 3653
SalI G'TCGA_C
Cuts at: 690 690
Size: 3653
SapI GCTCTTCn'nnn_
Cuts at: 191 1676 1886 191
Size: 1485 210 1958
Fragments arranged by size:
1958 1485 210
SbfI CC_TGCA'GG
Cuts at: 700 700
Size: 3653
ScaI AGT'ACT
Cuts at: 3170 3170
Size: 3653
SexAI A'CCwGG_T
Cuts at: 644 644
Size: 3653
SfcI C'TryA_G
Cuts at: 33 190 435 556 696 1381 2374 2565 33
Size: 157 245 121 140 685 993 191 1121
Fragments arranged by size:
1121 993 685 245 191 157 140 121
SfoI GGC'GCC
Cuts at: 1085 1333 1085
Size: 248 3405
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3653
SmaI CCC'GGG
Cuts at: 675 675
Size: 3653
SmlI C'TyrA_G
Cuts at: 808 1723 2215 2477 808
Size: 915 492 262 1984
Fragments arranged by size:
1984 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3653
SphI G_CATG'C
Cuts at: 706 1206 706
Size: 500 3153
StyI C'CwwG_G
Cuts at: 530 787 1141 530
Size: 257 354 3042
Fragments arranged by size:
3042 354 257
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1236 1369 1828 2037 1236
Size: 133 459 209 2852
Fragments arranged by size:
2852 459 209 133
TatI w'GTAC_w
Cuts at: 3168 3168
Size: 3653
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3170 3202 3170
Size: 32 3621
Tth111I GACn'n_nGTC
Cuts at: 1450 1450
Size: 3653
XbaI T'CTAG_A
Cuts at: 684 684
Size: 3653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 162 165 384 1148 162
Size: 3 219 764 2667
Fragments arranged by size:
2667 764 219 3
XmaI C'CCGG_G
Cuts at: 673 673
Size: 3653
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsgI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PshAI PsiI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI