Restriction map for
pF1KE0483
(Circular) (Six-base) MAPSORT of: pF1KE0483.seq Check: 7215 from: 1 to: 4142
pF1KE0483 4142 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 747 747
Size: 4142
AccI GT'mk_AC
Cuts at: 1180 1180
Size: 4142
Acc65I G'GTAC_C
Cuts at: 1158 1158
Size: 4142
AclI AA'CG_TT
Cuts at: 3982 3982
Size: 4142
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 904 1967 2399 3146 3414 904
Size: 1063 432 747 268 1632
Fragments arranged by size:
1632 1063 747 432 268
AflIII A'CryG_T
Cuts at: 1494 2598 3575 1494
Size: 1104 977 2061
Fragments arranged by size:
2061 1104 977
AgeI A'CCGG_T
Cuts at: 2530 2530
Size: 4142
AlwNI CAG_nnn'CTG
Cuts at: 466 607 1447 3014 466
Size: 141 840 1567 1594
Fragments arranged by size:
1594 1567 840 141
ApaLI G'TGCA_C
Cuts at: 2912 2912
Size: 4142
ApoI r'AATT_y
Cuts at: 241 325 1146 3495 3751 241
Size: 84 821 2349 256 632
Fragments arranged by size:
2349 821 632 256 84
AseI AT'TA_AT
Cuts at: 20 20
Size: 4142
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4142
AvaI C'yCGr_G
Cuts at: 1162 1162
Size: 4142
BamHI G'GATC_C
Cuts at: 1167 1167
Size: 4142
BanI G'GyrC_C
Cuts at: 1158 1572 1820 1855 1158
Size: 414 248 35 3445
Fragments arranged by size:
3445 414 248 35
BanII G_rGCy'C
Cuts at: 1156 2186 1156
Size: 1030 3112
BbeI G_GCGC'C
Cuts at: 1576 1824 1576
Size: 248 3894
BbsI GAAGACnn'nnnn_
Cuts at: 191 542 709 191
Size: 351 167 3624
Fragments arranged by size:
3624 351 167
BciVI GTATCCnnnnn_n'
Cuts at: 2040 2801 4142 2040
Size: 761 1341 2040
Fragments arranged by size:
2040 1341 761
BclI T'GATC_A
Cuts at: 1662 1662
Size: 4142
BglII A'GATC_T
Cuts at: 207 261 1657 3694 207
Size: 54 1396 2037 655
Fragments arranged by size:
2037 1396 655 54
BlpI GC'TnA_GC
Cuts at: 1254 1254
Size: 4142
Bme1580I G_kGCm'C
Cuts at: 294 1767 1860 2916 294
Size: 1473 93 1056 1520
Fragments arranged by size:
1520 1473 1056 93
BmgBI CAC'GTC
Cuts at: 189 189
Size: 4142
BmrI ACTGGGnnnn_n'
Cuts at: 1040 1354 1435 1770 3914 1040
Size: 314 81 335 2144 1268
Fragments arranged by size:
2144 1268 335 314 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2509 2509
Size: 4142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 392 961 392
Size: 569 3573
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1318 2197 2689 2987 1318
Size: 879 492 298 2473
Fragments arranged by size:
2473 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3846 3846
Size: 4142
BsaAI yAC'GTr
Cuts at: 1495 1495
Size: 4142
BsaBI GATnn'nnATC
Cuts at: 6 1656 3693 6
Size: 1650 2037 455
Fragments arranged by size:
2037 1650 455
BsaHI Gr'CG_yC
Cuts at: 1573 1821 2523 4019 1573
Size: 248 702 1496 1696
Fragments arranged by size:
1696 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1331 1852 2530 2804 2951 3362 1331
Size: 521 678 274 147 411 2111
Fragments arranged by size:
2111 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1063 1093 1063
Size: 30 4112
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 164 431 729 164
Size: 267 298 3577
Fragments arranged by size:
3577 298 267
BseYI C'CCAG_C
Cuts at: 405 466 813 965 1567 2325 2902 3398 405
Size: 61 347 152 602 758 577 496 1149
Fragments arranged by size:
1149 758 602 577 496 347 152 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 775 775
Size: 4142
BsiEI CG_ry'CG
Cuts at: 104 1684 1730 2938 104
Size: 1580 46 1208 1308
Fragments arranged by size:
1580 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1156 1934 2124 2916 1156
Size: 778 190 792 2382
Fragments arranged by size:
2382 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 294 1156 1767 1860 1934 2124 2186 2916 294
Size: 862 611 93 74 190 62 730 1520
Fragments arranged by size:
1520 862 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3362 3362
Size: 4142
BspHI T'CATG_A
Cuts at: 3318 3318
Size: 4142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 747 1045 1192 1406 1708 2089 747
Size: 298 147 214 302 381 2800
Fragments arranged by size:
2800 381 302 298 214 147
BsrBI CCG'CTC
Cuts at: 50 2434 2488 3972 4137 50
Size: 2384 54 1484 165 55
Fragments arranged by size:
2384 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2054 2054
Size: 4142
BsrFI r'CCGG_y
Cuts at: 2140 2321 2530 2140
Size: 181 209 3752
Fragments arranged by size:
3752 209 181
BsrGI T'GTAC_A
Cuts at: 1125 1125
Size: 4142
BssSI C'ACGA_G
Cuts at: 2413 2771 2413
Size: 358 3784
BstAPI GCAn_nnn'nTGC
Cuts at: 1519 1519
Size: 4142
BstBI TT'CG_AA
Cuts at: 2503 2503
Size: 4142
BstEII G'GTnAC_C
Cuts at: 471 471
Size: 4142
BstXI CCAn_nnnn'nTGG
Cuts at: 933 1014 933
Size: 81 4061
BstYI r'GATC_y
Cuts at: 1 207 261 1167 1634 1657 1992 2238 3250
Size: 206 54 906 467 23 335 246 1012
Cuts at: 3250 3336 3348 3694 1
Size: 86 12 346 449
Fragments arranged by size:
1012 906 467 449 346 335 246 206
86 54 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 805 2092 2273 2436 805
Size: 1287 181 163 2511
Fragments arranged by size:
2511 1287 181 163
BtsI GCAGTG_nn'
Cuts at: 1385 1385
Size: 4142
DraI TTT'AAA
Cuts at: 1141 1141
Size: 4142
DrdI GACnn_nn'nnGTC
Cuts at: 1848 2706 1848
Size: 858 3284
EaeI y'GGCC_r
Cuts at: 347 698 729 854 1727 1901 2292 2319 3664
Size: 351 31 125 873 174 391 27 1345
Cuts at: 3664 347
Size: 825
Fragments arranged by size:
1345 873 825 391 351 174 125 31
27
EagI C'GGCC_G
Cuts at: 1727 1727
Size: 4142
EarI CTCTTCn'nnn_
Cuts at: 575 908 2165 2375 575
Size: 333 1257 210 2342
Fragments arranged by size:
2342 1257 333 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 136 547 819 2350 2660 2806 3952 136
Size: 411 272 1531 310 146 1146 326
Fragments arranged by size:
1531 1146 411 326 310 272 146
EcoICRI GAG'CTC
Cuts at: 1154 1154
Size: 4142
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 392 904 961 1967 2399 3146 3414 392
Size: 512 57 1006 432 747 268 1120
Fragments arranged by size:
1120 1006 747 512 432 268 57
EcoO109I rG'GnC_Cy
Cuts at: 1034 1281 1034
Size: 247 3895
EcoRI G'AATT_C
Cuts at: 1146 1146
Size: 4142
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2161 2161
Size: 4142
FspI TGC'GCA
Cuts at: 1923 1923
Size: 4142
HaeII r_GCGC'y
Cuts at: 1576 1824 2846 3825 1576
Size: 248 1022 979 1893
Fragments arranged by size:
1893 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1984 2014 2016 2046 1984
Size: 30 2 30 4080
Fragments arranged by size:
4080 30 30 2
HincII GTy'rAC
Cuts at: 1181 1181
Size: 4142
KasI G'GCGC_C
Cuts at: 1572 1820 1572
Size: 248 3894
KpnI G_GTAC'C
Cuts at: 1162 1162
Size: 4142
MluI A'CGCG_T
Cuts at: 3575 3575
Size: 4142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 476 2813 2997 476
Size: 2337 184 1621
Fragments arranged by size:
2337 1621 184
MscI TGG'CCA
Cuts at: 349 700 731 856 1903 349
Size: 351 31 125 1047 2588
Fragments arranged by size:
2588 1047 351 125 31
MslI CAynn'nnrTG
Cuts at: 2131 2258 2131
Size: 127 4015
MspA1I CmG'CkG
Cuts at: 442 466 604 1253 1567 1927 2940 3185 442
Size: 24 138 649 314 360 1013 245 1399
Fragments arranged by size:
1399 1013 649 360 314 245 138 24
NaeI GCC'GGC
Cuts at: 2323 2323
Size: 4142
NarI GG'CG_CC
Cuts at: 1573 1821 1573
Size: 248 3894
NcoI C'CATG_G
Cuts at: 107 107
Size: 4142
NgoMIV G'CCGG_C
Cuts at: 2321 2321
Size: 4142
NspI r_CATG'y
Cuts at: 1078 1195 1695 2602 1078
Size: 117 500 907 2618
Fragments arranged by size:
2618 907 500 117
PciI A'CATG_T
Cuts at: 2598 2598
Size: 4142
PfoI T'CCnGG_A
Cuts at: 742 742
Size: 4142
PmeI GTTT'AAAC
Cuts at: 1141 1141
Size: 4142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3281 3313 3281
Size: 32 4110
PpuMI rG'GwC_Cy
Cuts at: 1034 1034
Size: 4142
PstI C_TGCA'G
Cuts at: 1189 1874 1189
Size: 685 3457
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4142
PvuII CAG'CTG
Cuts at: 466 604 1567 1927 466
Size: 138 963 360 2681
Fragments arranged by size:
2681 963 360 138
RsrII CG'GwC_CG
Cuts at: 2337 2337
Size: 4142
SacI G_AGCT'C
Cuts at: 1156 1156
Size: 4142
SalI G'TCGA_C
Cuts at: 1179 1179
Size: 4142
SapI GCTCTTCn'nnn_
Cuts at: 2165 2375 2165
Size: 210 3932
SbfI CC_TGCA'GG
Cuts at: 1189 1189
Size: 4142
ScaI AGT'ACT
Cuts at: 3659 3659
Size: 4142
SfcI C'TryA_G
Cuts at: 33 1185 1870 2863 3054 33
Size: 1152 685 993 191 1121
Fragments arranged by size:
1152 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1574 1822 1574
Size: 248 3894
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4142
SmaI CCC'GGG
Cuts at: 1164 1164
Size: 4142
SmlI C'TyrA_G
Cuts at: 1297 2212 2704 2966 1297
Size: 915 492 262 2473
Fragments arranged by size:
2473 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4142
SphI G_CATG'C
Cuts at: 1195 1695 1195
Size: 500 3642
SspI AAT'ATT
Cuts at: 230 230
Size: 4142
StuI AGG'CCT
Cuts at: 769 931 769
Size: 162 3980
StyI C'CwwG_G
Cuts at: 107 332 840 926 932 1276 1630 107
Size: 225 508 86 6 344 354 2619
Fragments arranged by size:
2619 508 354 344 225 86 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 979 1725 1858 2317 2526 979
Size: 746 133 459 209 2595
Fragments arranged by size:
2595 746 459 209 133
TatI w'GTAC_w
Cuts at: 1125 3657 1125
Size: 2532 1610
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1063 1095 3659 3691 1063
Size: 32 2564 32 1514
Fragments arranged by size:
2564 1514 32 32
Tth111I GACn'n_nGTC
Cuts at: 595 1939 595
Size: 1344 2798
XbaI T'CTAG_A
Cuts at: 1173 1173
Size: 4142
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 339 693 1637 339
Size: 354 944 2844
Fragments arranged by size:
2844 944 354
XmaI C'CCGG_G
Cuts at: 1162 1162
Size: 4142
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
Bpu10I BsiWI BsmI BsmBI BssHII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
XmnI ZraI