Restriction map for
pF1KE0479
(Circular) (Six-base) MAPSORT of: pF1KE0479.seq Check: 785 from: 1 to: 4058
pF1KE0479 4058 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 1096 1096
Size: 4058
Acc65I G'GTAC_C
Cuts at: 1074 1074
Size: 4058
AclI AA'CG_TT
Cuts at: 3898 3898
Size: 4058
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 1883 2315 3062 3330 293
Size: 1590 432 747 268 1021
Fragments arranged by size:
1590 1021 747 432 268
AfeI AGC'GCT
Cuts at: 415 415
Size: 4058
AflIII A'CryG_T
Cuts at: 755 1410 2514 3491 755
Size: 655 1104 977 1322
Fragments arranged by size:
1322 1104 977 655
AgeI A'CCGG_T
Cuts at: 2446 2446
Size: 4058
AlwNI CAG_nnn'CTG
Cuts at: 535 1363 2930 535
Size: 828 1567 1663
Fragments arranged by size:
1663 1567 828
ApaLI G'TGCA_C
Cuts at: 144 2828 144
Size: 2684 1374
ApoI r'AATT_y
Cuts at: 400 1062 3411 3667 400
Size: 662 2349 256 791
Fragments arranged by size:
2349 791 662 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4058
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4058
AvaI C'yCGr_G
Cuts at: 1078 1078
Size: 4058
BamHI G'GATC_C
Cuts at: 878 1083 878
Size: 205 3853
BanI G'GyrC_C
Cuts at: 920 1074 1488 1736 1771 920
Size: 154 414 248 35 3207
Fragments arranged by size:
3207 414 248 154 35
BanII G_rGCy'C
Cuts at: 386 1072 2102 386
Size: 686 1030 2342
Fragments arranged by size:
2342 1030 686
BbeI G_GCGC'C
Cuts at: 1492 1740 1492
Size: 248 3810
BbvCI CC'TCA_GC
Cuts at: 203 203
Size: 4058
BciVI GTATCCnnnnn_n'
Cuts at: 1956 2717 4058 1956
Size: 761 1341 1956
Fragments arranged by size:
1956 1341 761
BclI T'GATC_A
Cuts at: 291 897 1578 291
Size: 606 681 2771
Fragments arranged by size:
2771 681 606
BglII A'GATC_T
Cuts at: 1573 3610 1573
Size: 2037 2021
BlpI GC'TnA_GC
Cuts at: 1170 1170
Size: 4058
Bme1580I G_kGCm'C
Cuts at: 148 1683 1776 2832 148
Size: 1535 93 1056 1374
Fragments arranged by size:
1535 1374 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1270 1351 1686 3830 1270
Size: 81 335 2144 1498
Fragments arranged by size:
2144 1498 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2425 2425
Size: 4058
Bpu10I CC'TnA_GC
Cuts at: 203 203
Size: 4058
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 180 1234 2113 2605 2903 180
Size: 1054 879 492 298 1335
Fragments arranged by size:
1335 1054 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 602 859 3762 602
Size: 257 2903 898
Fragments arranged by size:
2903 898 257
BsaAI yAC'GTr
Cuts at: 1411 1411
Size: 4058
BsaBI GATnn'nnATC
Cuts at: 6 530 1572 3609 6
Size: 524 1042 2037 455
Fragments arranged by size:
2037 1042 524 455
BsaHI Gr'CG_yC
Cuts at: 1489 1737 2439 3935 1489
Size: 248 702 1496 1612
Fragments arranged by size:
1612 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1247 1768 2446 2720 2867 3278 1247
Size: 521 678 274 147 411 2027
Fragments arranged by size:
2027 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 502 643 670 985 502
Size: 141 27 315 3575
Fragments arranged by size:
3575 315 141 27
BseYI C'CCAG_C
Cuts at: 1483 2241 2818 3314 1483
Size: 758 577 496 2227
Fragments arranged by size:
2227 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 631 631
Size: 4058
BsiEI CG_ry'CG
Cuts at: 104 1600 1646 2854 104
Size: 1496 46 1208 1308
Fragments arranged by size:
1496 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 148 386 1072 1850 2040 2832 148
Size: 238 686 778 190 792 1374
Fragments arranged by size:
1374 792 778 686 238 190
BsmI GAATG_Cn'
Cuts at: 720 720
Size: 4058
Bsp1286I G_dGCh'C
Cuts at: 148 386 1072 1683 1776 1850 2040 2102 2832
Size: 238 686 611 93 74 190 62 730
Cuts at: 2832 148
Size: 1374
Fragments arranged by size:
1374 730 686 611 238 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3278 3278
Size: 4058
BspHI T'CATG_A
Cuts at: 195 3234 195
Size: 3039 1019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 122 1108 1322 1624 2005 122
Size: 986 214 302 381 2175
Fragments arranged by size:
2175 986 381 302 214
BsrBI CCG'CTC
Cuts at: 50 579 2350 2404 3888 4053 50
Size: 529 1771 54 1484 165 55
Fragments arranged by size:
1771 1484 529 165 55 54
BsrDI GCAATG_nn'
Cuts at: 614 1970 614
Size: 1356 2702
BsrFI r'CCGG_y
Cuts at: 2056 2237 2446 2056
Size: 181 209 3668
Fragments arranged by size:
3668 209 181
BsrGI T'GTAC_A
Cuts at: 189 758 189
Size: 569 3489
BssSI C'ACGA_G
Cuts at: 2329 2687 2329
Size: 358 3700
BstAPI GCAn_nnn'nTGC
Cuts at: 1435 1435
Size: 4058
BstBI TT'CG_AA
Cuts at: 2419 2419
Size: 4058
BstXI CCAn_nnnn'nTGG
Cuts at: 924 924
Size: 4058
BstYI r'GATC_y
Cuts at: 1 531 878 1083 1550 1573 1908 2154 3166
Size: 530 347 205 467 23 335 246 1012
Cuts at: 3166 3252 3264 3610 1
Size: 86 12 346 449
Fragments arranged by size:
1012 530 467 449 347 346 335 246
205 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 653 653
Size: 4058
BtgI C'CryG_G
Cuts at: 107 107
Size: 4058
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2008 2189 2352 2008
Size: 181 163 3714
Fragments arranged by size:
3714 181 163
BtsI GCAGTG_nn'
Cuts at: 1301 1301
Size: 4058
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1002 1037 1002
Size: 35 4023
DraI TTT'AAA
Cuts at: 588 1057 588
Size: 469 3589
DrdI GACnn_nn'nnGTC
Cuts at: 367 1764 2622 367
Size: 1397 858 1803
Fragments arranged by size:
1803 1397 858
EaeI y'GGCC_r
Cuts at: 249 1643 1817 2208 2235 3580 249
Size: 1394 174 391 27 1345 727
Fragments arranged by size:
1394 1345 727 391 174 27
EagI C'GGCC_G
Cuts at: 1643 1643
Size: 4058
EarI CTCTTCn'nnn_
Cuts at: 302 734 857 2081 2291 302
Size: 432 123 1224 210 2069
Fragments arranged by size:
2069 1224 432 210 123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2266 2576 2722 3868 2266
Size: 310 146 1146 2456
Fragments arranged by size:
2456 1146 310 146
EcoICRI GAG'CTC
Cuts at: 384 1070 384
Size: 686 3372
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 1883 2315 3062 3330 293
Size: 1590 432 747 268 1021
Fragments arranged by size:
1590 1021 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 463 463
Size: 4058
EcoO109I rG'GnC_Cy
Cuts at: 1197 1197
Size: 4058
EcoRI G'AATT_C
Cuts at: 1062 1062
Size: 4058
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1008 2077 1008
Size: 1069 2989
FspI TGC'GCA
Cuts at: 1839 1839
Size: 4058
HaeII r_GCGC'y
Cuts at: 417 1492 1740 2762 3741 417
Size: 1075 248 1022 979 734
Fragments arranged by size:
1075 1022 979 734 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 276 308 1900 1930 1932 1962 276
Size: 32 1592 30 2 30 2372
Fragments arranged by size:
2372 1592 32 30 30 2
HincII GTy'rAC
Cuts at: 981 1097 981
Size: 116 3942
HindIII A'AGCT_T
Cuts at: 958 958
Size: 4058
KasI G'GCGC_C
Cuts at: 1488 1736 1488
Size: 248 3810
KpnI G_GTAC'C
Cuts at: 1078 1078
Size: 4058
MluI A'CGCG_T
Cuts at: 3491 3491
Size: 4058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2729 2913 2729
Size: 184 3874
MscI TGG'CCA
Cuts at: 251 1819 251
Size: 1568 2490
MslI CAynn'nnrTG
Cuts at: 227 2047 2174 227
Size: 1820 127 2111
Fragments arranged by size:
2111 1820 127
MspA1I CmG'CkG
Cuts at: 297 1169 1483 1843 2856 3101 297
Size: 872 314 360 1013 245 1254
Fragments arranged by size:
1254 1013 872 360 314 245
NaeI GCC'GGC
Cuts at: 2239 2239
Size: 4058
NarI GG'CG_CC
Cuts at: 1489 1737 1489
Size: 248 3810
NcoI C'CATG_G
Cuts at: 107 107
Size: 4058
NgoMIV G'CCGG_C
Cuts at: 2237 2237
Size: 4058
NspI r_CATG'y
Cuts at: 759 1111 1611 2518 759
Size: 352 500 907 2299
Fragments arranged by size:
2299 907 500 352
PciI A'CATG_T
Cuts at: 755 2514 755
Size: 1759 2299
PflMI CCAn_nnn'nTGG
Cuts at: 875 875
Size: 4058
PmeI GTTT'AAAC
Cuts at: 1057 1057
Size: 4058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3197 3229 3197
Size: 32 4026
PstI C_TGCA'G
Cuts at: 1105 1790 1105
Size: 685 3373
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4058
PvuII CAG'CTG
Cuts at: 297 1483 1843 297
Size: 1186 360 2512
Fragments arranged by size:
2512 1186 360
RsrII CG'GwC_CG
Cuts at: 2253 2253
Size: 4058
SacI G_AGCT'C
Cuts at: 386 1072 386
Size: 686 3372
SalI G'TCGA_C
Cuts at: 1095 1095
Size: 4058
SapI GCTCTTCn'nnn_
Cuts at: 2081 2291 2081
Size: 210 3848
SbfI CC_TGCA'GG
Cuts at: 1105 1105
Size: 4058
ScaI AGT'ACT
Cuts at: 3575 3575
Size: 4058
SexAI A'CCwGG_T
Cuts at: 923 923
Size: 4058
SfcI C'TryA_G
Cuts at: 33 1101 1786 2779 2970 33
Size: 1068 685 993 191 1121
Fragments arranged by size:
1121 1068 993 685 191
SfoI GGC'GCC
Cuts at: 1490 1738 1490
Size: 248 3810
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4058
SmaI CCC'GGG
Cuts at: 1080 1080
Size: 4058
SmlI C'TyrA_G
Cuts at: 159 1213 2128 2620 2882 159
Size: 1054 915 492 262 1335
Fragments arranged by size:
1335 1054 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4058
SphI G_CATG'C
Cuts at: 1111 1611 1111
Size: 500 3558
StuI AGG'CCT
Cuts at: 1030 1030
Size: 4058
StyI C'CwwG_G
Cuts at: 107 623 965 1192 1546 107
Size: 516 342 227 354 2619
Fragments arranged by size:
2619 516 354 342 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 256 1641 1774 2233 2442 256
Size: 1385 133 459 209 1872
Fragments arranged by size:
1872 1385 459 209 133
TatI w'GTAC_w
Cuts at: 189 684 758 3573 189
Size: 495 74 2815 674
Fragments arranged by size:
2815 674 495 74
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3575 3607 3575
Size: 32 4026
Tth111I GACn'n_nGTC
Cuts at: 1855 1855
Size: 4058
XbaI T'CTAG_A
Cuts at: 735 1089 735
Size: 354 3704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 949 1553 949
Size: 604 3454
XmaI C'CCGG_G
Cuts at: 1078 1078
Size: 4058
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmgBI
BmtI BpmI BsaXI BsiWI BsmBI BssHII BstEII BstZ17I
ClaI DraIII EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI