Restriction map for
pF1KE0478
(Circular) (Six-base) MAPSORT of: pF1KE0478.seq Check: 4575 from: 1 to: 4238
pF1KE0478 4238 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 838 838
Size: 4238
AccI GT'mk_AC
Cuts at: 1276 1276
Size: 4238
Acc65I G'GTAC_C
Cuts at: 1254 1254
Size: 4238
AclI AA'CG_TT
Cuts at: 4078 4078
Size: 4238
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2063 2495 3242 3510 2063
Size: 432 747 268 2791
Fragments arranged by size:
2791 747 432 268
AfeI AGC'GCT
Cuts at: 791 912 791
Size: 121 4117
AflIII A'CryG_T
Cuts at: 560 1590 2694 3671 560
Size: 1030 1104 977 1127
Fragments arranged by size:
1127 1104 1030 977
AgeI A'CCGG_T
Cuts at: 2626 2626
Size: 4238
AleI CACnn'nnGTG
Cuts at: 823 823
Size: 4238
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 176 176
Size: 4238
AlwNI CAG_nnn'CTG
Cuts at: 423 1543 3110 423
Size: 1120 1567 1551
Fragments arranged by size:
1567 1551 1120
ApaI G_GGCC'C
Cuts at: 1221 1221
Size: 4238
ApaLI G'TGCA_C
Cuts at: 151 1163 3008 151
Size: 1012 1845 1381
Fragments arranged by size:
1845 1381 1012
ApoI r'AATT_y
Cuts at: 1242 3591 3847 1242
Size: 2349 256 1633
Fragments arranged by size:
2349 1633 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4238
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
AvaI C'yCGr_G
Cuts at: 475 1063 1220 1258 475
Size: 588 157 38 3455
Fragments arranged by size:
3455 588 157 38
BamHI G'GATC_C
Cuts at: 1263 1263
Size: 4238
BanI G'GyrC_C
Cuts at: 430 496 978 1041 1254 1668 1916 1951 430
Size: 66 482 63 213 414 248 35 2717
Fragments arranged by size:
2717 482 414 248 213 66 63 35
BanII G_rGCy'C
Cuts at: 1135 1221 1252 2282 1135
Size: 86 31 1030 3091
Fragments arranged by size:
3091 1030 86 31
BbeI G_GCGC'C
Cuts at: 434 1045 1672 1920 434
Size: 611 627 248 2752
Fragments arranged by size:
2752 627 611 248
BbvCI CC'TCA_GC
Cuts at: 230 874 230
Size: 644 3594
BciVI GTATCCnnnnn_n'
Cuts at: 2136 2897 4238 2136
Size: 761 1341 2136
Fragments arranged by size:
2136 1341 761
BclI T'GATC_A
Cuts at: 1758 1758
Size: 4238
BfrBI ATG'CAT
Cuts at: 923 923
Size: 4238
BglI GCCn_nnn'nGGC
Cuts at: 688 838 688
Size: 150 4088
BglII A'GATC_T
Cuts at: 1753 3790 1753
Size: 2037 2201
BlpI GC'TnA_GC
Cuts at: 416 1350 416
Size: 934 3304
Bme1580I G_kGCm'C
Cuts at: 155 1167 1221 1863 1956 3012 155
Size: 1012 54 642 93 1056 1381
Fragments arranged by size:
1381 1056 1012 642 93 54
BmgBI CAC'GTC
Cuts at: 156 156
Size: 4238
BmrI ACTGGGnnnn_n'
Cuts at: 736 1450 1531 1866 4010 736
Size: 714 81 335 2144 964
Fragments arranged by size:
2144 964 714 335 81
BmtI G_CTAG'C
Cuts at: 448 448
Size: 4238
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2605 2605
Size: 4238
Bpu10I CC'TnA_GC
Cuts at: 230 874 230
Size: 644 3594
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 595 607 1414 2293 2785 3083 445
Size: 150 12 807 879 492 298 1600
Fragments arranged by size:
1600 879 807 492 298 150 12
BsaI GGTCTCn'nnnn_
Cuts at: 301 3942 301
Size: 3641 597
BsaAI yAC'GTr
Cuts at: 1591 1591
Size: 4238
BsaBI GATnn'nnATC
Cuts at: 6 1752 3789 6
Size: 1746 2037 455
Fragments arranged by size:
2037 1746 455
BsaHI Gr'CG_yC
Cuts at: 335 431 814 1042 1669 1917 2619 4115 335
Size: 96 383 228 627 248 702 1496 458
Fragments arranged by size:
1496 702 627 458 383 248 228 96
BsaWI w'CCGG_w
Cuts at: 1427 1948 2626 2900 3047 3458 1427
Size: 521 678 274 147 411 2207
Fragments arranged by size:
2207 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 103 133 489 519 103
Size: 30 356 30 3822
Fragments arranged by size:
3822 356 30 30
BseYI C'CCAG_C
Cuts at: 964 1155 1663 2421 2998 3494 964
Size: 191 508 758 577 496 1708
Fragments arranged by size:
1708 758 577 508 496 191
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 874 874
Size: 4238
BsiEI CG_ry'CG
Cuts at: 104 1780 1826 3034 104
Size: 1676 46 1208 1308
Fragments arranged by size:
1676 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 155 1013 1167 1252 2030 2220 3012 155
Size: 858 154 85 778 190 792 1381
Fragments arranged by size:
1381 858 792 778 190 154 85
BsmBI CGTCTCn'nnnn_
Cuts at: 162 805 162
Size: 643 3595
Bsp1286I G_dGCh'C
Cuts at: 155 1013 1135 1167 1221 1252 1863 1956 2030
Size: 858 122 32 54 31 611 93 74
Cuts at: 2030 2220 2282 3012 155
Size: 190 62 730 1381
Fragments arranged by size:
1381 858 730 611 190 122 93 74
62 54 32 31
BspEI T'CCGG_A
Cuts at: 3458 3458
Size: 4238
BspHI T'CATG_A
Cuts at: 3414 3414
Size: 4238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 838 1288 1502 1804 2185 838
Size: 450 214 302 381 2891
Fragments arranged by size:
2891 450 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2530 2584 4068 4233 50
Size: 2480 54 1484 165 55
Fragments arranged by size:
2480 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 635 2150 635
Size: 1515 2723
BsrFI r'CCGG_y
Cuts at: 289 493 2236 2417 2626 289
Size: 204 1743 181 209 1901
Fragments arranged by size:
1901 1743 209 204 181
BssHII G'CGCG_C
Cuts at: 114 891 114
Size: 777 3461
BssSI C'ACGA_G
Cuts at: 1126 2509 2867 1126
Size: 1383 358 2497
Fragments arranged by size:
2497 1383 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1615 1615
Size: 4238
BstBI TT'CG_AA
Cuts at: 2599 2599
Size: 4238
BstYI r'GATC_y
Cuts at: 1 281 1263 1730 1753 2088 2334 3346 3432
Size: 280 982 467 23 335 246 1012 86
Cuts at: 3432 3444 3790 1
Size: 12 346 449
Fragments arranged by size:
1012 982 467 449 346 335 280 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 343 107
Size: 236 4002
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2188 2369 2532 2188
Size: 181 163 3894
Fragments arranged by size:
3894 181 163
BtsI GCAGTG_nn'
Cuts at: 610 1481 610
Size: 871 3367
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 513 548 513
Size: 35 4203
DraI TTT'AAA
Cuts at: 1237 1237
Size: 4238
DrdI GACnn_nn'nnGTC
Cuts at: 1944 2802 1944
Size: 858 3380
EaeI y'GGCC_r
Cuts at: 773 1823 1997 2388 2415 3760 773
Size: 1050 174 391 27 1345 1251
Fragments arranged by size:
1345 1251 1050 391 174 27
EagI C'GGCC_G
Cuts at: 1823 1823
Size: 4238
EarI CTCTTCn'nnn_
Cuts at: 2261 2471 2261
Size: 210 4028
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2446 2756 2902 4048 2446
Size: 310 146 1146 2636
Fragments arranged by size:
2636 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1250 1250
Size: 4238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2063 2495 3242 3510 2063
Size: 432 747 268 2791
Fragments arranged by size:
2791 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 480 1217 1377 480
Size: 737 160 3341
Fragments arranged by size:
3341 737 160
EcoRI G'AATT_C
Cuts at: 1242 1242
Size: 4238
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2257 2257
Size: 4238
FspI TGC'GCA
Cuts at: 796 2019 796
Size: 1223 3015
HaeII r_GCGC'y
Cuts at: 434 793 914 938 1045 1672 1920 2942 3921
Size: 359 121 24 107 627 248 1022 979
Cuts at: 3921 434
Size: 751
Fragments arranged by size:
1022 979 751 627 359 248 121 107
24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 103 135 735 767 2080 2110 2112 2142 103
Size: 32 600 32 1313 30 2 30 2199
Fragments arranged by size:
2199 1313 600 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1277 1277
Size: 4238
KasI G'GCGC_C
Cuts at: 430 1041 1668 1916 430
Size: 611 627 248 2752
Fragments arranged by size:
2752 627 611 248
KpnI G_GTAC'C
Cuts at: 1258 1258
Size: 4238
MluI A'CGCG_T
Cuts at: 3671 3671
Size: 4238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2909 3093 2909
Size: 184 4054
MscI TGG'CCA
Cuts at: 1999 1999
Size: 4238
MslI CAynn'nnrTG
Cuts at: 805 823 2227 2354 805
Size: 18 1404 127 2689
Fragments arranged by size:
2689 1404 127 18
MspA1I CmG'CkG
Cuts at: 147 206 345 510 1022 1349 1663 2023 3036
Size: 59 139 165 512 327 314 360 1013
Cuts at: 3036 3281 147
Size: 245 1104
Fragments arranged by size:
1104 1013 512 360 327 314 245 165
139 59
NaeI GCC'GGC
Cuts at: 291 2419 291
Size: 2128 2110
NarI GG'CG_CC
Cuts at: 431 1042 1669 1917 431
Size: 611 627 248 2752
Fragments arranged by size:
2752 627 611 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4238
NgoMIV G'CCGG_C
Cuts at: 289 2417 289
Size: 2128 2110
NheI G'CTAG_C
Cuts at: 444 444
Size: 4238
NsiI A_TGCA'T
Cuts at: 925 925
Size: 4238
NspI r_CATG'y
Cuts at: 927 1291 1791 2698 927
Size: 364 500 907 2467
Fragments arranged by size:
2467 907 500 364
PciI A'CATG_T
Cuts at: 2694 2694
Size: 4238
PfoI T'CCnGG_A
Cuts at: 720 720
Size: 4238
PmeI GTTT'AAAC
Cuts at: 1237 1237
Size: 4238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 458 490 3377 3409 458
Size: 32 2887 32 1287
Fragments arranged by size:
2887 1287 32 32
PpuMI rG'GwC_Cy
Cuts at: 480 480
Size: 4238
PspOMI G'GGCC_C
Cuts at: 1217 1217
Size: 4238
PspXI vC'TCGA_Gb
Cuts at: 475 475
Size: 4238
PstI C_TGCA'G
Cuts at: 270 602 1285 1970 270
Size: 332 683 685 2538
Fragments arranged by size:
2538 685 683 332
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4238
PvuII CAG'CTG
Cuts at: 1022 1663 2023 1022
Size: 641 360 3237
Fragments arranged by size:
3237 641 360
RsrII CG'GwC_CG
Cuts at: 2433 2433
Size: 4238
SacI G_AGCT'C
Cuts at: 1252 1252
Size: 4238
SacII CC_GC'GG
Cuts at: 346 346
Size: 4238
SalI G'TCGA_C
Cuts at: 1275 1275
Size: 4238
SapI GCTCTTCn'nnn_
Cuts at: 2261 2471 2261
Size: 210 4028
SbfI CC_TGCA'GG
Cuts at: 1285 1285
Size: 4238
ScaI AGT'ACT
Cuts at: 3755 3755
Size: 4238
SfcI C'TryA_G
Cuts at: 33 266 598 1281 1966 2959 3150 33
Size: 233 332 683 685 993 191 1121
Fragments arranged by size:
1121 993 685 683 332 233 191
SfoI GGC'GCC
Cuts at: 432 1043 1670 1918 432
Size: 611 627 248 2752
Fragments arranged by size:
2752 627 611 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
SmaI CCC'GGG
Cuts at: 1222 1260 1222
Size: 38 4200
SmlI C'TyrA_G
Cuts at: 460 475 610 622 1393 2308 2800 3062 460
Size: 15 135 12 771 915 492 262 1636
Fragments arranged by size:
1636 915 771 492 262 135 15 12
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4238
SphI G_CATG'C
Cuts at: 1291 1791 1291
Size: 500 3738
StuI AGG'CCT
Cuts at: 620 620
Size: 4238
StyI C'CwwG_G
Cuts at: 107 1212 1372 1726 107
Size: 1105 160 354 2619
Fragments arranged by size:
2619 1105 354 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1821 1954 2413 2622 1821
Size: 133 459 209 3437
Fragments arranged by size:
3437 459 209 133
TatI w'GTAC_w
Cuts at: 3753 3753
Size: 4238
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 489 521 1187 1219 3755 3787 489
Size: 32 666 32 2536 32 940
Fragments arranged by size:
2536 940 666 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 361 1176 2035 361
Size: 815 859 2564
Fragments arranged by size:
2564 859 815
XbaI T'CTAG_A
Cuts at: 1269 1269
Size: 4238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1733 1733
Size: 4238
XhoI C'TCGA_G
Cuts at: 475 475
Size: 4238
XmaI C'CCGG_G
Cuts at: 1220 1258 1220
Size: 38 4200
XmnI GAAnn'nnTTC
Cuts at: 674 1096 674
Size: 422 3816
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NsiI NspI PciI PfoI PmeI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BbsI
BcgI BpmI BseRI BsiWI BsmI BsrGI BstEII BstXI
BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NotI NruI PacI PasI
PflMI PmlI PshAI PsiI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI ZraI