Restriction map for
pF1KE0474
(Circular) (Six-base) MAPSORT of: pF1KE0474.seq Check: 7628 from: 1 to: 3614
pF1KE0474 3614 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 652 652
Size: 3614
Acc65I G'GTAC_C
Cuts at: 630 630
Size: 3614
AclI AA'CG_TT
Cuts at: 3454 3454
Size: 3614
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 561 1439 1871 2618 2886 561
Size: 878 432 747 268 1289
Fragments arranged by size:
1289 878 747 432 268
AflII C'TTAA_G
Cuts at: 208 208
Size: 3614
AflIII A'CryG_T
Cuts at: 966 2070 3047 966
Size: 1104 977 1533
Fragments arranged by size:
1533 1104 977
AgeI A'CCGG_T
Cuts at: 2002 2002
Size: 3614
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 428 428
Size: 3614
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 275 307 275
Size: 32 3582
AlwNI CAG_nnn'CTG
Cuts at: 124 919 2486 124
Size: 795 1567 1252
Fragments arranged by size:
1567 1252 795
ApaLI G'TGCA_C
Cuts at: 2384 2384
Size: 3614
ApoI r'AATT_y
Cuts at: 370 618 2967 3223 370
Size: 248 2349 256 761
Fragments arranged by size:
2349 761 256 248
AseI AT'TA_AT
Cuts at: 20 20
Size: 3614
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3614
AvaI C'yCGr_G
Cuts at: 634 634
Size: 3614
BamHI G'GATC_C
Cuts at: 639 639
Size: 3614
BanI G'GyrC_C
Cuts at: 429 630 1044 1292 1327 429
Size: 201 414 248 35 2716
Fragments arranged by size:
2716 414 248 201 35
BanII G_rGCy'C
Cuts at: 321 628 1658 321
Size: 307 1030 2277
Fragments arranged by size:
2277 1030 307
BbeI G_GCGC'C
Cuts at: 1048 1296 1048
Size: 248 3366
BciVI GTATCCnnnnn_n'
Cuts at: 1512 2273 3614 1512
Size: 761 1341 1512
Fragments arranged by size:
1512 1341 761
BclI T'GATC_A
Cuts at: 1134 1134
Size: 3614
BglII A'GATC_T
Cuts at: 131 1129 3166 131
Size: 998 2037 579
Fragments arranged by size:
2037 998 579
BlpI GC'TnA_GC
Cuts at: 726 726
Size: 3614
Bme1580I G_kGCm'C
Cuts at: 432 1239 1332 2388 432
Size: 807 93 1056 1658
Fragments arranged by size:
1658 1056 807 93
BmrI ACTGGGnnnn_n'
Cuts at: 826 907 1242 3386 826
Size: 81 335 2144 1054
Fragments arranged by size:
2144 1054 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1981 1981
Size: 3614
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 251
Size: 3614
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 790 1669 2161 2459 790
Size: 879 492 298 1945
Fragments arranged by size:
1945 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3318 3318
Size: 3614
BsaAI yAC'GTr
Cuts at: 967 967
Size: 3614
BsaBI GATnn'nnATC
Cuts at: 6 1128 3165 6
Size: 1122 2037 455
Fragments arranged by size:
2037 1122 455
BsaHI Gr'CG_yC
Cuts at: 1045 1293 1995 3491 1045
Size: 248 702 1496 1168
Fragments arranged by size:
1496 1168 702 248
BsaWI w'CCGG_w
Cuts at: 803 1324 2002 2276 2423 2834 803
Size: 521 678 274 147 411 1583
Fragments arranged by size:
1583 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1039 1797 2374 2870 1039
Size: 758 577 496 1783
Fragments arranged by size:
1783 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1156 1202 2410 104
Size: 1052 46 1208 1308
Fragments arranged by size:
1308 1208 1052 46
BsiHKAI G_wGCw'C
Cuts at: 321 628 1406 1596 2388 321
Size: 307 778 190 792 1547
Fragments arranged by size:
1547 792 778 307 190
Bsp1286I G_dGCh'C
Cuts at: 321 432 628 1239 1332 1406 1596 1658 2388
Size: 111 196 611 93 74 190 62 730
Cuts at: 2388 321
Size: 1547
Fragments arranged by size:
1547 730 611 196 190 111 93 74
62
BspEI T'CCGG_A
Cuts at: 2834 2834
Size: 3614
BspHI T'CATG_A
Cuts at: 2790 2790
Size: 3614
BspMI ACCTGCnnnn'nnnn_
Cuts at: 664 878 1180 1561 664
Size: 214 302 381 2717
Fragments arranged by size:
2717 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1906 1960 3444 3609 50
Size: 1856 54 1484 165 55
Fragments arranged by size:
1856 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 594 1526 594
Size: 932 2682
BsrFI r'CCGG_y
Cuts at: 1612 1793 2002 1612
Size: 181 209 3224
Fragments arranged by size:
3224 209 181
BssSI C'ACGA_G
Cuts at: 1885 2243 1885
Size: 358 3256
BstAPI GCAn_nnn'nTGC
Cuts at: 991 991
Size: 3614
BstBI TT'CG_AA
Cuts at: 1975 1975
Size: 3614
BstYI r'GATC_y
Cuts at: 1 131 639 1106 1129 1464 1710 2722 2808
Size: 130 508 467 23 335 246 1012 86
Cuts at: 2808 2820 3166 1
Size: 12 346 449
Fragments arranged by size:
1012 508 467 449 346 335 246 130
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1564 1745 1908 1564
Size: 181 163 3270
Fragments arranged by size:
3270 181 163
BtsI GCAGTG_nn'
Cuts at: 407 857 407
Size: 450 3164
DraI TTT'AAA
Cuts at: 613 613
Size: 3614
DrdI GACnn_nn'nnGTC
Cuts at: 1320 2178 1320
Size: 858 2756
EaeI y'GGCC_r
Cuts at: 301 1199 1373 1764 1791 3136 301
Size: 898 174 391 27 1345 779
Fragments arranged by size:
1345 898 779 391 174 27
EagI C'GGCC_G
Cuts at: 1199 1199
Size: 3614
EarI CTCTTCn'nnn_
Cuts at: 374 1637 1847 374
Size: 1263 210 2141
Fragments arranged by size:
2141 1263 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1822 2132 2278 3424 1822
Size: 310 146 1146 2012
Fragments arranged by size:
2012 1146 310 146
EcoICRI GAG'CTC
Cuts at: 319 626 319
Size: 307 3307
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 561 1439 1871 2618 2886 251
Size: 310 878 432 747 268 979
Fragments arranged by size:
979 878 747 432 310 268
EcoO109I rG'GnC_Cy
Cuts at: 753 753
Size: 3614
EcoRI G'AATT_C
Cuts at: 618 618
Size: 3614
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1633 1633
Size: 3614
FspI TGC'GCA
Cuts at: 1395 1395
Size: 3614
HaeII r_GCGC'y
Cuts at: 1048 1296 2318 3297 1048
Size: 248 1022 979 1365
Fragments arranged by size:
1365 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1456 1486 1488 1518 1456
Size: 30 2 30 3552
Fragments arranged by size:
3552 30 30 2
HincII GTy'rAC
Cuts at: 653 653
Size: 3614
KasI G'GCGC_C
Cuts at: 1044 1292 1044
Size: 248 3366
KpnI G_GTAC'C
Cuts at: 634 634
Size: 3614
MluI A'CGCG_T
Cuts at: 3047 3047
Size: 3614
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2285 2469 2285
Size: 184 3430
MscI TGG'CCA
Cuts at: 303 1375 303
Size: 1072 2542
MslI CAynn'nnrTG
Cuts at: 599 1603 1730 599
Size: 1004 127 2483
Fragments arranged by size:
2483 1004 127
MspA1I CmG'CkG
Cuts at: 534 725 1039 1399 2412 2657 534
Size: 191 314 360 1013 245 1491
Fragments arranged by size:
1491 1013 360 314 245 191
NaeI GCC'GGC
Cuts at: 1795 1795
Size: 3614
NarI GG'CG_CC
Cuts at: 1045 1293 1045
Size: 248 3366
NdeI CA'TA_TG
Cuts at: 255 255
Size: 3614
NgoMIV G'CCGG_C
Cuts at: 1793 1793
Size: 3614
NspI r_CATG'y
Cuts at: 150 667 1167 2074 150
Size: 517 500 907 1690
Fragments arranged by size:
1690 907 517 500
PciI A'CATG_T
Cuts at: 2070 2070
Size: 3614
PmeI GTTT'AAAC
Cuts at: 613 613
Size: 3614
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2753 2785 2753
Size: 32 3582
PsiI TTA'TAA
Cuts at: 496 496
Size: 3614
PstI C_TGCA'G
Cuts at: 661 1346 661
Size: 685 2929
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3614
PvuII CAG'CTG
Cuts at: 534 1039 1399 534
Size: 505 360 2749
Fragments arranged by size:
2749 505 360
RsrII CG'GwC_CG
Cuts at: 1809 1809
Size: 3614
SacI G_AGCT'C
Cuts at: 321 628 321
Size: 307 3307
SalI G'TCGA_C
Cuts at: 651 651
Size: 3614
SapI GCTCTTCn'nnn_
Cuts at: 374 1637 1847 374
Size: 1263 210 2141
Fragments arranged by size:
2141 1263 210
SbfI CC_TGCA'GG
Cuts at: 661 661
Size: 3614
ScaI AGT'ACT
Cuts at: 3131 3131
Size: 3614
SfcI C'TryA_G
Cuts at: 33 657 1342 2335 2526 33
Size: 624 685 993 191 1121
Fragments arranged by size:
1121 993 685 624 191
SfoI GGC'GCC
Cuts at: 1046 1294 1046
Size: 248 3366
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3614
SmaI CCC'GGG
Cuts at: 636 636
Size: 3614
SmlI C'TyrA_G
Cuts at: 208 769 1684 2176 2438 208
Size: 561 915 492 262 1384
Fragments arranged by size:
1384 915 561 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3614
SphI G_CATG'C
Cuts at: 667 1167 667
Size: 500 3114
StyI C'CwwG_G
Cuts at: 748 1102 748
Size: 354 3260
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1197 1330 1789 1998 1197
Size: 133 459 209 2813
Fragments arranged by size:
2813 459 209 133
TatI w'GTAC_w
Cuts at: 3129 3129
Size: 3614
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3131 3163 3131
Size: 32 3582
Tth111I GACn'n_nGTC
Cuts at: 1411 1411
Size: 3614
XbaI T'CTAG_A
Cuts at: 294 645 294
Size: 351 3263
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1109 1109
Size: 3614
XmaI C'CCGG_G
Cuts at: 634 634
Size: 3614
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NspI PciI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI