Restriction map for
pF1KE0472
(Circular) (Six-base) MAPSORT of: pF1KE0472.seq Check: 3963 from: 1 to: 3671
pF1KE0472 3671 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 709 709
Size: 3671
Acc65I G'GTAC_C
Cuts at: 687 687
Size: 3671
AclI AA'CG_TT
Cuts at: 3511 3511
Size: 3671
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1496 1928 2675 2943 1496
Size: 432 747 268 2224
Fragments arranged by size:
2224 747 432 268
AflIII A'CryG_T
Cuts at: 1023 2127 3104 1023
Size: 1104 977 1590
Fragments arranged by size:
1590 1104 977
AgeI A'CCGG_T
Cuts at: 2059 2059
Size: 3671
AlwNI CAG_nnn'CTG
Cuts at: 502 976 2543 502
Size: 474 1567 1630
Fragments arranged by size:
1630 1567 474
ApaLI G'TGCA_C
Cuts at: 2441 2441
Size: 3671
ApoI r'AATT_y
Cuts at: 675 3024 3280 675
Size: 2349 256 1066
Fragments arranged by size:
2349 1066 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3671
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3671
AvaI C'yCGr_G
Cuts at: 691 691
Size: 3671
BamHI G'GATC_C
Cuts at: 696 696
Size: 3671
BanI G'GyrC_C
Cuts at: 687 1101 1349 1384 687
Size: 414 248 35 2974
Fragments arranged by size:
2974 414 248 35
BanII G_rGCy'C
Cuts at: 685 1715 685
Size: 1030 2641
BbeI G_GCGC'C
Cuts at: 1105 1353 1105
Size: 248 3423
BciVI GTATCCnnnnn_n'
Cuts at: 1569 2330 3671 1569
Size: 761 1341 1569
Fragments arranged by size:
1569 1341 761
BclI T'GATC_A
Cuts at: 377 1191 377
Size: 814 2857
BglII A'GATC_T
Cuts at: 1186 3223 1186
Size: 2037 1634
BlpI GC'TnA_GC
Cuts at: 783 783
Size: 3671
Bme1580I G_kGCm'C
Cuts at: 1296 1389 2445 1296
Size: 93 1056 2522
Fragments arranged by size:
2522 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 883 964 1299 3443 883
Size: 81 335 2144 1111
Fragments arranged by size:
2144 1111 335 81
BmtI G_CTAG'C
Cuts at: 619 619
Size: 3671
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 201 300 2038 201
Size: 99 1738 1834
Fragments arranged by size:
1834 1738 99
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 168
Size: 3671
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 1726 2218 2516 847
Size: 879 492 298 2002
Fragments arranged by size:
2002 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3375 3375
Size: 3671
BsaAI yAC'GTr
Cuts at: 1024 1024
Size: 3671
BsaBI GATnn'nnATC
Cuts at: 6 1185 3222 6
Size: 1179 2037 455
Fragments arranged by size:
2037 1179 455
BsaHI Gr'CG_yC
Cuts at: 1102 1350 2052 3548 1102
Size: 248 702 1496 1225
Fragments arranged by size:
1496 1225 702 248
BsaWI w'CCGG_w
Cuts at: 860 1381 2059 2333 2480 2891 860
Size: 521 678 274 147 411 1640
Fragments arranged by size:
1640 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 274 304 274
Size: 30 3641
BseYI C'CCAG_C
Cuts at: 421 591 595 1096 1854 2431 2927 421
Size: 170 4 501 758 577 496 1165
Fragments arranged by size:
1165 758 577 501 496 170 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 219 407 219
Size: 188 3483
BsiEI CG_ry'CG
Cuts at: 104 1213 1259 2467 104
Size: 1109 46 1208 1308
Fragments arranged by size:
1308 1208 1109 46
BsiHKAI G_wGCw'C
Cuts at: 281 685 1463 1653 2445 281
Size: 404 778 190 792 1507
Fragments arranged by size:
1507 792 778 404 190
Bsp1286I G_dGCh'C
Cuts at: 281 685 1296 1389 1463 1653 1715 2445 281
Size: 404 611 93 74 190 62 730 1507
Fragments arranged by size:
1507 730 611 404 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2891 2891
Size: 3671
BspHI T'CATG_A
Cuts at: 266 2847 266
Size: 2581 1090
BspMI ACCTGCnnnn'nnnn_
Cuts at: 721 935 1237 1618 721
Size: 214 302 381 2774
Fragments arranged by size:
2774 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1963 2017 3501 3666 50
Size: 1913 54 1484 165 55
Fragments arranged by size:
1913 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1583 1583
Size: 3671
BsrFI r'CCGG_y
Cuts at: 1669 1850 2059 1669
Size: 181 209 3281
Fragments arranged by size:
3281 209 181
BssSI C'ACGA_G
Cuts at: 1942 2300 1942
Size: 358 3313
BstAPI GCAn_nnn'nTGC
Cuts at: 1048 1048
Size: 3671
BstBI TT'CG_AA
Cuts at: 2032 2032
Size: 3671
BstYI r'GATC_y
Cuts at: 1 696 1163 1186 1521 1767 2779 2865 2877
Size: 695 467 23 335 246 1012 86 12
Cuts at: 2877 3223 1
Size: 346 449
Fragments arranged by size:
1012 695 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 315 315
Size: 3671
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 348 1621 1802 1965 348
Size: 1273 181 163 2054
Fragments arranged by size:
2054 1273 181 163
BtsI GCAGTG_nn'
Cuts at: 503 914 503
Size: 411 3260
DraI TTT'AAA
Cuts at: 670 670
Size: 3671
DrdI GACnn_nn'nnGTC
Cuts at: 1377 2235 1377
Size: 858 2813
EaeI y'GGCC_r
Cuts at: 1256 1430 1821 1848 3193 1256
Size: 174 391 27 1345 1734
Fragments arranged by size:
1734 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1256 1256
Size: 3671
EarI CTCTTCn'nnn_
Cuts at: 170 1694 1904 170
Size: 1524 210 1937
Fragments arranged by size:
1937 1524 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1879 2189 2335 3481 1879
Size: 310 146 1146 2069
Fragments arranged by size:
2069 1146 310 146
EcoICRI GAG'CTC
Cuts at: 683 683
Size: 3671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 1496 1928 2675 2943 168
Size: 1328 432 747 268 896
Fragments arranged by size:
1328 896 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 810 810
Size: 3671
EcoRI G'AATT_C
Cuts at: 675 675
Size: 3671
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1690 1690
Size: 3671
FspI TGC'GCA
Cuts at: 1452 1452
Size: 3671
HaeII r_GCGC'y
Cuts at: 1105 1353 2375 3354 1105
Size: 248 1022 979 1422
Fragments arranged by size:
1422 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1513 1543 1545 1575 1513
Size: 30 2 30 3609
Fragments arranged by size:
3609 30 30 2
HincII GTy'rAC
Cuts at: 710 710
Size: 3671
HindIII A'AGCT_T
Cuts at: 369 369
Size: 3671
KasI G'GCGC_C
Cuts at: 1101 1349 1101
Size: 248 3423
KpnI G_GTAC'C
Cuts at: 691 691
Size: 3671
MluI A'CGCG_T
Cuts at: 3104 3104
Size: 3671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 621 2342 2526 621
Size: 1721 184 1766
Fragments arranged by size:
1766 1721 184
MscI TGG'CCA
Cuts at: 1432 1432
Size: 3671
MslI CAynn'nnrTG
Cuts at: 529 1660 1787 529
Size: 1131 127 2413
Fragments arranged by size:
2413 1131 127
MspA1I CmG'CkG
Cuts at: 595 782 1096 1456 2469 2714 595
Size: 187 314 360 1013 245 1552
Fragments arranged by size:
1552 1013 360 314 245 187
NaeI GCC'GGC
Cuts at: 1852 1852
Size: 3671
NarI GG'CG_CC
Cuts at: 1102 1350 1102
Size: 248 3423
NgoMIV G'CCGG_C
Cuts at: 1850 1850
Size: 3671
NheI G'CTAG_C
Cuts at: 615 615
Size: 3671
NspI r_CATG'y
Cuts at: 244 724 1224 2131 244
Size: 480 500 907 1784
Fragments arranged by size:
1784 907 500 480
PciI A'CATG_T
Cuts at: 2127 2127
Size: 3671
PflMI CCAn_nnn'nTGG
Cuts at: 194 498 194
Size: 304 3367
PmeI GTTT'AAAC
Cuts at: 670 670
Size: 3671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2810 2842 2810
Size: 32 3639
PstI C_TGCA'G
Cuts at: 306 447 718 1403 306
Size: 141 271 685 2574
Fragments arranged by size:
2574 685 271 141
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3671
PvuII CAG'CTG
Cuts at: 595 1096 1456 595
Size: 501 360 2810
Fragments arranged by size:
2810 501 360
RsrII CG'GwC_CG
Cuts at: 1866 1866
Size: 3671
SacI G_AGCT'C
Cuts at: 685 685
Size: 3671
SalI G'TCGA_C
Cuts at: 708 708
Size: 3671
SapI GCTCTTCn'nnn_
Cuts at: 1694 1904 1694
Size: 210 3461
SbfI CC_TGCA'GG
Cuts at: 718 718
Size: 3671
ScaI AGT'ACT
Cuts at: 3188 3188
Size: 3671
SfcI C'TryA_G
Cuts at: 33 302 356 443 714 1399 2392 2583 33
Size: 269 54 87 271 685 993 191 1121
Fragments arranged by size:
1121 993 685 271 269 191 87 54
SfoI GGC'GCC
Cuts at: 1103 1351 1103
Size: 248 3423
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3671
SmaI CCC'GGG
Cuts at: 693 693
Size: 3671
SmlI C'TyrA_G
Cuts at: 826 1741 2233 2495 826
Size: 915 492 262 2002
Fragments arranged by size:
2002 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3671
SphI G_CATG'C
Cuts at: 724 1224 724
Size: 500 3171
SspI AAT'ATT
Cuts at: 192 192
Size: 3671
StyI C'CwwG_G
Cuts at: 805 1159 805
Size: 354 3317
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1254 1387 1846 2055 1254
Size: 133 459 209 2870
Fragments arranged by size:
2870 459 209 133
TatI w'GTAC_w
Cuts at: 3186 3186
Size: 3671
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3188 3220 3188
Size: 32 3639
Tth111I GACn'n_nGTC
Cuts at: 1468 1468
Size: 3671
XbaI T'CTAG_A
Cuts at: 702 702
Size: 3671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1166 1166
Size: 3671
XmaI C'CCGG_G
Cuts at: 691 691
Size: 3671
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NheI NspI PciI PflMI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI Bpu10I BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NcoI NdeI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI
ZraI