Restriction map for
pF1KE0471
(Circular) (Six-base) MAPSORT of: pF1KE0471.seq Check: 1187 from: 1 to: 4430
pF1KE0471 4430 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 1468 1468
Size: 4430
Acc65I G'GTAC_C
Cuts at: 1446 1446
Size: 4430
AclI AA'CG_TT
Cuts at: 4270 4270
Size: 4430
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2255 2687 3434 3702 2255
Size: 432 747 268 2983
Fragments arranged by size:
2983 747 432 268
AflIII A'CryG_T
Cuts at: 1782 2886 3863 1782
Size: 1104 977 2349
Fragments arranged by size:
2349 1104 977
AgeI A'CCGG_T
Cuts at: 2818 2818
Size: 4430
AlwNI CAG_nnn'CTG
Cuts at: 1735 3302 1735
Size: 1567 2863
ApaI G_GGCC'C
Cuts at: 262 636 1219 262
Size: 374 583 3473
Fragments arranged by size:
3473 583 374
ApaLI G'TGCA_C
Cuts at: 3200 3200
Size: 4430
ApoI r'AATT_y
Cuts at: 361 442 649 1434 3783 4039 361
Size: 81 207 785 2349 256 752
Fragments arranged by size:
2349 785 752 256 207 81
AseI AT'TA_AT
Cuts at: 20 20
Size: 4430
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4430
AvaI C'yCGr_G
Cuts at: 1073 1322 1450 1073
Size: 249 128 4053
Fragments arranged by size:
4053 249 128
BamHI G'GATC_C
Cuts at: 924 1455 924
Size: 531 3899
BanI G'GyrC_C
Cuts at: 1099 1446 1860 2108 2143 1099
Size: 347 414 248 35 3386
Fragments arranged by size:
3386 414 347 248 35
BanII G_rGCy'C
Cuts at: 262 636 1093 1219 1444 2474 262
Size: 374 457 126 225 1030 2218
Fragments arranged by size:
2218 1030 457 374 225 126
BbeI G_GCGC'C
Cuts at: 1103 1864 2112 1103
Size: 761 248 3421
Fragments arranged by size:
3421 761 248
BbsI GAAGACnn'nnnn_
Cuts at: 779 779
Size: 4430
BciVI GTATCCnnnnn_n'
Cuts at: 2328 3089 4430 2328
Size: 761 1341 2328
Fragments arranged by size:
2328 1341 761
BclI T'GATC_A
Cuts at: 1950 1950
Size: 4430
BglI GCCn_nnn'nGGC
Cuts at: 640 739 1097 640
Size: 99 358 3973
Fragments arranged by size:
3973 358 99
BglII A'GATC_T
Cuts at: 1945 3982 1945
Size: 2037 2393
BlpI GC'TnA_GC
Cuts at: 1542 1542
Size: 4430
Bme1580I G_kGCm'C
Cuts at: 262 636 875 1219 2055 2148 3204 262
Size: 374 239 344 836 93 1056 1488
Fragments arranged by size:
1488 1056 836 374 344 239 93
BmrI ACTGGGnnnn_n'
Cuts at: 265 552 1642 1723 2058 4202 265
Size: 287 1090 81 335 2144 493
Fragments arranged by size:
2144 1090 493 335 287 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2797 2797
Size: 4430
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 405 475 813 833 840 157
Size: 248 70 338 20 7 3747
Fragments arranged by size:
3747 338 248 70 20 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1275 1606 2485 2977 3275 1275
Size: 331 879 492 298 2430
Fragments arranged by size:
2430 879 492 331 298
BsaI GGTCTCn'nnnn_
Cuts at: 223 395 739 4134 223
Size: 172 344 3395 519
Fragments arranged by size:
3395 519 344 172
BsaAI yAC'GTr
Cuts at: 1783 1783
Size: 4430
BsaBI GATnn'nnATC
Cuts at: 6 1944 3981 6
Size: 1938 2037 455
Fragments arranged by size:
2037 1938 455
BsaHI Gr'CG_yC
Cuts at: 1100 1861 2109 2811 4307 1100
Size: 761 248 702 1496 1223
Fragments arranged by size:
1496 1223 761 702 248
BsaWI w'CCGG_w
Cuts at: 1619 2140 2818 3092 3239 3650 1619
Size: 521 678 274 147 411 2399
Fragments arranged by size:
2399 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 130 160 563 593 130
Size: 30 403 30 3967
Fragments arranged by size:
3967 403 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 241 670 1018 1382 241
Size: 429 348 364 3289
Fragments arranged by size:
3289 429 364 348
BseYI C'CCAG_C
Cuts at: 1855 2613 3190 3686 1855
Size: 758 577 496 2599
Fragments arranged by size:
2599 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 601 168
Size: 433 3997
BsiEI CG_ry'CG
Cuts at: 104 1972 2018 3226 104
Size: 1868 46 1208 1308
Fragments arranged by size:
1868 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 349 1444 2222 2412 3204 349
Size: 1095 778 190 792 1575
Fragments arranged by size:
1575 1095 792 778 190
BsmI GAATG_Cn'
Cuts at: 768 768
Size: 4430
Bsp1286I G_dGCh'C
Cuts at: 262 349 636 875 1093 1219 1444 2055 2148
Size: 87 287 239 218 126 225 611 93
Cuts at: 2148 2222 2412 2474 3204 262
Size: 74 190 62 730 1488
Fragments arranged by size:
1488 730 611 287 239 225 218 190
126 93 87 74 62
BspEI T'CCGG_A
Cuts at: 3650 3650
Size: 4430
BspHI T'CATG_A
Cuts at: 3606 3606
Size: 4430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1480 1694 1996 2377 1480
Size: 214 302 381 3533
Fragments arranged by size:
3533 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1183 2722 2776 4260 4425 50
Size: 1133 1539 54 1484 165 55
Fragments arranged by size:
1539 1484 1133 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2342 2342
Size: 4430
BsrFI r'CCGG_y
Cuts at: 1263 2428 2609 2818 1263
Size: 1165 181 209 2875
Fragments arranged by size:
2875 1165 209 181
BssHII G'CGCG_C
Cuts at: 269 269
Size: 4430
BssSI C'ACGA_G
Cuts at: 2701 3059 2701
Size: 358 4072
BstAPI GCAn_nnn'nTGC
Cuts at: 1807 1807
Size: 4430
BstBI TT'CG_AA
Cuts at: 2791 2791
Size: 4430
BstXI CCAn_nnnn'nTGG
Cuts at: 120 542 798 1123 120
Size: 422 256 325 3427
Fragments arranged by size:
3427 422 325 256
BstYI r'GATC_y
Cuts at: 1 608 924 1455 1922 1945 2280 2526 3538
Size: 607 316 531 467 23 335 246 1012
Cuts at: 3538 3624 3636 3982 1
Size: 86 12 346 449
Fragments arranged by size:
1012 607 531 467 449 346 335 316
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 929 1064 929
Size: 135 4295
BtgI C'CryG_G
Cuts at: 119 767 1025 1154 119
Size: 648 258 129 3395
Fragments arranged by size:
3395 648 258 129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 139 2380 2561 2724 139
Size: 2241 181 163 1845
Fragments arranged by size:
2241 1845 181 163
BtsI GCAGTG_nn'
Cuts at: 581 875 1673 581
Size: 294 798 3338
Fragments arranged by size:
3338 798 294
DraI TTT'AAA
Cuts at: 428 1429 428
Size: 1001 3429
DrdI GACnn_nn'nnGTC
Cuts at: 2136 2994 2136
Size: 858 3572
EaeI y'GGCC_r
Cuts at: 1125 2015 2189 2580 2607 3952 1125
Size: 890 174 391 27 1345 1603
Fragments arranged by size:
1603 1345 890 391 174 27
EagI C'GGCC_G
Cuts at: 2015 2015
Size: 4430
EarI CTCTTCn'nnn_
Cuts at: 290 2453 2663 290
Size: 2163 210 2057
Fragments arranged by size:
2163 2057 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 472 2638 2948 3094 4240 472
Size: 2166 310 146 1146 662
Fragments arranged by size:
2166 1146 662 310 146
EcoICRI GAG'CTC
Cuts at: 1442 1442
Size: 4430
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 405 475 813 833 840 2255 2687 3434
Size: 248 70 338 20 7 1415 432 747
Cuts at: 3434 3702 157
Size: 268 885
Fragments arranged by size:
1415 885 747 432 338 268 248 70
20 7
EcoNI CCTnn'n_nnAGG
Cuts at: 136 319 136
Size: 183 4247
EcoO109I rG'GnC_Cy
Cuts at: 176 243 632 1048 1216 1317 1569 176
Size: 67 389 416 168 101 252 3037
Fragments arranged by size:
3037 416 389 252 168 101 67
EcoRI G'AATT_C
Cuts at: 361 442 649 1434 361
Size: 81 207 785 3357
Fragments arranged by size:
3357 785 207 81
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1227 2449 1227
Size: 1222 3208
FspI TGC'GCA
Cuts at: 2211 2211
Size: 4430
HaeII r_GCGC'y
Cuts at: 1103 1864 2112 3134 4113 1103
Size: 761 248 1022 979 1420
Fragments arranged by size:
1420 1022 979 761 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2272 2302 2304 2334 2272
Size: 30 2 30 4368
Fragments arranged by size:
4368 30 30 2
HincII GTy'rAC
Cuts at: 1239 1469 1239
Size: 230 4200
KasI G'GCGC_C
Cuts at: 1099 1860 2108 1099
Size: 761 248 3421
Fragments arranged by size:
3421 761 248
KpnI G_GTAC'C
Cuts at: 1450 1450
Size: 4430
MluI A'CGCG_T
Cuts at: 3863 3863
Size: 4430
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 926 3101 3285 926
Size: 2175 184 2071
Fragments arranged by size:
2175 2071 184
MscI TGG'CCA
Cuts at: 1127 2191 1127
Size: 1064 3366
MslI CAynn'nnrTG
Cuts at: 124 2419 2546 124
Size: 2295 127 2008
Fragments arranged by size:
2295 2008 127
MspA1I CmG'CkG
Cuts at: 646 1541 1855 2215 3228 3473 646
Size: 895 314 360 1013 245 1603
Fragments arranged by size:
1603 1013 895 360 314 245
NaeI GCC'GGC
Cuts at: 2611 2611
Size: 4430
NarI GG'CG_CC
Cuts at: 1100 1861 2109 1100
Size: 761 248 3421
Fragments arranged by size:
3421 761 248
NcoI C'CATG_G
Cuts at: 119 1025 119
Size: 906 3524
NgoMIV G'CCGG_C
Cuts at: 2609 2609
Size: 4430
NspI r_CATG'y
Cuts at: 1483 1983 2890 1483
Size: 500 907 3023
Fragments arranged by size:
3023 907 500
PciI A'CATG_T
Cuts at: 2886 2886
Size: 4430
PflMI CCAn_nnn'nTGG
Cuts at: 758 758
Size: 4430
PfoI T'CCnGG_A
Cuts at: 446 829 446
Size: 383 4047
PmeI GTTT'AAAC
Cuts at: 1429 1429
Size: 4430
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3569 3601 3569
Size: 32 4398
PpuMI rG'GwC_Cy
Cuts at: 176 243 1048 176
Size: 67 805 3558
Fragments arranged by size:
3558 805 67
PspOMI G'GGCC_C
Cuts at: 258 632 1215 258
Size: 374 583 3473
Fragments arranged by size:
3473 583 374
PstI C_TGCA'G
Cuts at: 1477 2162 1477
Size: 685 3745
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4430
PvuII CAG'CTG
Cuts at: 1855 2215 1855
Size: 360 4070
RsrII CG'GwC_CG
Cuts at: 2625 2625
Size: 4430
SacI G_AGCT'C
Cuts at: 1444 1444
Size: 4430
SalI G'TCGA_C
Cuts at: 1467 1467
Size: 4430
SanDI GG'GwC_CC
Cuts at: 1048 1048
Size: 4430
SapI GCTCTTCn'nnn_
Cuts at: 2453 2663 2453
Size: 210 4220
SbfI CC_TGCA'GG
Cuts at: 1477 1477
Size: 4430
ScaI AGT'ACT
Cuts at: 3947 3947
Size: 4430
SfcI C'TryA_G
Cuts at: 33 396 1473 2158 3151 3342 33
Size: 363 1077 685 993 191 1121
Fragments arranged by size:
1121 1077 993 685 363 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 640 640
Size: 4430
SfoI GGC'GCC
Cuts at: 1101 1862 2110 1101
Size: 761 248 3421
Fragments arranged by size:
3421 761 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4430
SmaI CCC'GGG
Cuts at: 1075 1452 1075
Size: 377 4053
SmlI C'TyrA_G
Cuts at: 1290 1585 2500 2992 3254 1290
Size: 295 915 492 262 2466
Fragments arranged by size:
2466 915 492 295 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4430
SphI G_CATG'C
Cuts at: 1483 1983 1483
Size: 500 3930
SrfI GCCC'GGGC
Cuts at: 1075 1075
Size: 4430
StyI C'CwwG_G
Cuts at: 119 1025 1564 1918 119
Size: 906 539 354 2631
Fragments arranged by size:
2631 906 539 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 925 1152 2013 2146 2605 2814 925
Size: 227 861 133 459 209 2541
Fragments arranged by size:
2541 861 459 227 209 133
TatI w'GTAC_w
Cuts at: 3945 3945
Size: 4430
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 130 146 162 178 563 595 3947 3979 130
Size: 16 16 16 385 32 3352 32 581
Fragments arranged by size:
3352 581 385 32 32 16 16 16
Tth111I GACn'n_nGTC
Cuts at: 2227 2227
Size: 4430
XbaI T'CTAG_A
Cuts at: 1461 1461
Size: 4430
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1925 1925
Size: 4430
XmaI C'CCGG_G
Cuts at: 1073 1450 1073
Size: 377 4053
XmnI GAAnn'nnTTC
Cuts at: 442 442
Size: 4430
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsiWI BsmBI BsrGI BstEII BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PshAI
PsiI PspXI PsrI SacII SexAI SgrAI SnaBI SspI
StuI SwaI XhoI ZraI