Restriction map for
pF1KE0468
(Circular) (Six-base) MAPSORT of: pF1KE0468.seq Check: 5758 from: 1 to: 4268
pF1KE0468 4268 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AatII G_ACGT'C
Cuts at: 715 715
Size: 4268
AccI GT'mk_AC
Cuts at: 1306 1306
Size: 4268
Acc65I G'GTAC_C
Cuts at: 1284 1284
Size: 4268
AclI AA'CG_TT
Cuts at: 4108 4108
Size: 4268
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 768 901 2093 2525 3272 3540 768
Size: 133 1192 432 747 268 1496
Fragments arranged by size:
1496 1192 747 432 268 133
AflIII A'CryG_T
Cuts at: 332 1620 2724 3701 332
Size: 1288 1104 977 899
Fragments arranged by size:
1288 1104 977 899
AgeI A'CCGG_T
Cuts at: 2656 2656
Size: 4268
AhdI GACnn_n'nnGTC
Cuts at: 181 181
Size: 4268
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 707 707
Size: 4268
AlwNI CAG_nnn'CTG
Cuts at: 178 1573 3140 178
Size: 1395 1567 1306
Fragments arranged by size:
1567 1395 1306
ApaI G_GGCC'C
Cuts at: 901 1172 901
Size: 271 3997
ApaLI G'TGCA_C
Cuts at: 319 3038 319
Size: 2719 1549
ApoI r'AATT_y
Cuts at: 389 1272 3621 3877 389
Size: 883 2349 256 780
Fragments arranged by size:
2349 883 780 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4268
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4268
AvaI C'yCGr_G
Cuts at: 1084 1172 1288 1084
Size: 88 116 4064
Fragments arranged by size:
4064 116 88
BamHI G'GATC_C
Cuts at: 140 1293 140
Size: 1153 3115
BanI G'GyrC_C
Cuts at: 400 470 596 649 733 986 1201 1284 1698
Size: 70 126 53 84 253 215 83 414
Cuts at: 1698 1946 1981 400
Size: 248 35 2687
Fragments arranged by size:
2687 414 253 248 215 126 84 83
70 53 35
BanII G_rGCy'C
Cuts at: 901 1172 1282 2312 901
Size: 271 110 1030 2857
Fragments arranged by size:
2857 1030 271 110
BbeI G_GCGC'C
Cuts at: 474 600 737 990 1702 1950 474
Size: 126 137 253 712 248 2792
Fragments arranged by size:
2792 712 253 248 137 126
BbsI GAAGACnn'nnnn_
Cuts at: 774 774
Size: 4268
BbvCI CC'TCA_GC
Cuts at: 1128 1128
Size: 4268
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1242 1276 1242
Size: 34 4234
BciVI GTATCCnnnnn_n'
Cuts at: 2166 2927 4268 2166
Size: 761 1341 2166
Fragments arranged by size:
2166 1341 761
BclI T'GATC_A
Cuts at: 422 1788 422
Size: 1366 2902
BglII A'GATC_T
Cuts at: 494 1783 3820 494
Size: 1289 2037 942
Fragments arranged by size:
2037 1289 942
BlpI GC'TnA_GC
Cuts at: 954 1380 954
Size: 426 3842
Bme1580I G_kGCm'C
Cuts at: 323 901 1085 1172 1206 1225 1893 1986 3042
Size: 578 184 87 34 19 668 93 1056
Cuts at: 3042 323
Size: 1549
Fragments arranged by size:
1549 1056 668 578 184 93 87 34
19
BmrI ACTGGGnnnn_n'
Cuts at: 1072 1255 1480 1561 1896 4040 1072
Size: 183 225 81 335 2144 1300
Fragments arranged by size:
2144 1300 335 225 183 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2635 2635
Size: 4268
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 155 697 1170 155
Size: 542 473 3253
Fragments arranged by size:
3253 542 473
Bpu10I CC'TnA_GC
Cuts at: 1128 1128
Size: 4268
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 1036 1444 2323 2815 3113 286
Size: 750 408 879 492 298 1441
Fragments arranged by size:
1441 879 750 492 408 298
BsaI GGTCTCn'nnnn_
Cuts at: 117 706 733 3972 117
Size: 589 27 3239 413
Fragments arranged by size:
3239 589 413 27
BsaAI yAC'GTr
Cuts at: 1621 1621
Size: 4268
BsaBI GATnn'nnATC
Cuts at: 6 1782 3819 6
Size: 1776 2037 455
Fragments arranged by size:
2037 1776 455
BsaHI Gr'CG_yC
Cuts at: 471 597 712 734 987 1699 1947 2649 4145
Size: 126 115 22 253 712 248 702 1496
Cuts at: 4145 471
Size: 594
Fragments arranged by size:
1496 712 702 594 253 248 126 115
22
BsaWI w'CCGG_w
Cuts at: 1457 1978 2656 2930 3077 3488 1457
Size: 521 678 274 147 411 2237
Fragments arranged by size:
2237 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 610 640 1021 1051 610
Size: 30 381 30 3827
Fragments arranged by size:
3827 381 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 580 583 580
Size: 3 4265
BseYI C'CCAG_C
Cuts at: 264 454 900 917 1693 2451 3028 3524 264
Size: 190 446 17 776 758 577 496 1008
Fragments arranged by size:
1008 776 758 577 496 446 190 17
BsiEI CG_ry'CG
Cuts at: 104 1810 1856 3064 104
Size: 1706 46 1208 1308
Fragments arranged by size:
1706 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 323 1282 2060 2250 3042 323
Size: 959 778 190 792 1549
Fragments arranged by size:
1549 959 792 778 190
BsmI GAATG_Cn'
Cuts at: 164 164
Size: 4268
Bsp1286I G_dGCh'C
Cuts at: 323 901 1085 1172 1206 1225 1282 1893 1986
Size: 578 184 87 34 19 57 611 93
Cuts at: 1986 2060 2250 2312 3042 323
Size: 74 190 62 730 1549
Fragments arranged by size:
1549 730 611 578 190 184 93 87
74 62 57 34 19
BspEI T'CCGG_A
Cuts at: 3488 3488
Size: 4268
BspHI T'CATG_A
Cuts at: 3444 3444
Size: 4268
BspMI ACCTGCnnnn'nnnn_
Cuts at: 186 1318 1532 1834 2215 186
Size: 1132 214 302 381 2239
Fragments arranged by size:
2239 1132 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2560 2614 4098 4263 50
Size: 2510 54 1484 165 55
Fragments arranged by size:
2510 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2180 2180
Size: 4268
BsrFI r'CCGG_y
Cuts at: 160 2266 2447 2656 160
Size: 2106 181 209 1772
Fragments arranged by size:
2106 1772 209 181
BssSI C'ACGA_G
Cuts at: 2539 2897 2539
Size: 358 3910
BstAPI GCAn_nnn'nTGC
Cuts at: 532 1645 532
Size: 1113 3155
BstBI TT'CG_AA
Cuts at: 2629 2629
Size: 4268
BstEII G'GTnAC_C
Cuts at: 1104 1143 1104
Size: 39 4229
BstXI CCAn_nnnn'nTGG
Cuts at: 519 519
Size: 4268
BstYI r'GATC_y
Cuts at: 1 140 237 494 671 819 1293 1760 1783
Size: 139 97 257 177 148 474 467 23
Cuts at: 1783 2118 2364 3376 3462 3474 3820 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 474 467 449 346 335 257 246
177 148 139 97 86 23 12
BtgI C'CryG_G
Cuts at: 107 275 396 107
Size: 168 121 3979
Fragments arranged by size:
3979 168 121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 276 2218 2399 2562 276
Size: 1942 181 163 1982
Fragments arranged by size:
1982 1942 181 163
BtsI GCAGTG_nn'
Cuts at: 542 605 979 1511 542
Size: 63 374 532 3299
Fragments arranged by size:
3299 532 374 63
DraI TTT'AAA
Cuts at: 1267 1267
Size: 4268
DrdI GACnn_nn'nnGTC
Cuts at: 1102 1974 2832 1102
Size: 872 858 2538
Fragments arranged by size:
2538 872 858
EaeI y'GGCC_r
Cuts at: 125 1853 2027 2418 2445 3790 125
Size: 1728 174 391 27 1345 603
Fragments arranged by size:
1728 1345 603 391 174 27
EagI C'GGCC_G
Cuts at: 1853 1853
Size: 4268
EarI CTCTTCn'nnn_
Cuts at: 217 2291 2501 217
Size: 2074 210 1984
Fragments arranged by size:
2074 1984 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2476 2786 2932 4078 2476
Size: 310 146 1146 2666
Fragments arranged by size:
2666 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1280 1280
Size: 4268
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 155 697 768 901 1170 2093 2525 3272 3540
Size: 542 71 133 269 923 432 747 268
Cuts at: 3540 155
Size: 883
Fragments arranged by size:
923 883 747 542 432 269 268 133
71
EcoO109I rG'GnC_Cy
Cuts at: 175 876 897 1168 1169 1194 1256 1407 175
Size: 701 21 271 1 25 62 151 3036
Fragments arranged by size:
3036 701 271 151 62 25 21 1
EcoRI G'AATT_C
Cuts at: 1272 1272
Size: 4268
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2287 2287
Size: 4268
FspI TGC'GCA
Cuts at: 2049 2049
Size: 4268
HaeII r_GCGC'y
Cuts at: 474 600 737 990 997 1702 1950 2972 3951
Size: 126 137 253 7 705 248 1022 979
Cuts at: 3951 474
Size: 791
Fragments arranged by size:
1022 979 791 705 253 248 137 126
7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 167 199 1021 1053 2110 2140 2142 2172 167
Size: 32 822 32 1057 30 2 30 2263
Fragments arranged by size:
2263 1057 822 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1307 1307
Size: 4268
KasI G'GCGC_C
Cuts at: 470 596 733 986 1698 1946 470
Size: 126 137 253 712 248 2792
Fragments arranged by size:
2792 712 253 248 137 126
KpnI G_GTAC'C
Cuts at: 1288 1288
Size: 4268
MluI A'CGCG_T
Cuts at: 3701 3701
Size: 4268
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 536 2939 3123 536
Size: 2403 184 1681
Fragments arranged by size:
2403 1681 184
MscI TGG'CCA
Cuts at: 127 2029 127
Size: 1902 2366
MslI CAynn'nnrTG
Cuts at: 637 2257 2384 637
Size: 1620 127 2521
Fragments arranged by size:
2521 1620 127
MspA1I CmG'CkG
Cuts at: 1072 1379 1693 2053 3066 3311 1072
Size: 307 314 360 1013 245 2029
Fragments arranged by size:
2029 1013 360 314 307 245
NaeI GCC'GGC
Cuts at: 162 2449 162
Size: 2287 1981
NarI GG'CG_CC
Cuts at: 471 597 734 987 1699 1947 471
Size: 126 137 253 712 248 2792
Fragments arranged by size:
2792 712 253 248 137 126
NcoI C'CATG_G
Cuts at: 107 107
Size: 4268
NgoMIV G'CCGG_C
Cuts at: 160 2447 160
Size: 2287 1981
NspI r_CATG'y
Cuts at: 231 310 336 642 1321 1821 2728 231
Size: 79 26 306 679 500 907 1771
Fragments arranged by size:
1771 907 679 500 306 79 26
PciI A'CATG_T
Cuts at: 332 2724 332
Size: 2392 1876
PfoI T'CCnGG_A
Cuts at: 171 674 171
Size: 503 3765
PmeI GTTT'AAAC
Cuts at: 1267 1267
Size: 4268
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 430 462 3407 3439 430
Size: 32 2945 32 1259
Fragments arranged by size:
2945 1259 32 32
PpuMI rG'GwC_Cy
Cuts at: 175 876 1256 175
Size: 701 380 3187
Fragments arranged by size:
3187 701 380
PspOMI G'GGCC_C
Cuts at: 897 1168 897
Size: 271 3997
PstI C_TGCA'G
Cuts at: 367 549 1315 2000 367
Size: 182 766 685 2635
Fragments arranged by size:
2635 766 685 182
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4268
PvuII CAG'CTG
Cuts at: 1072 1693 2053 1072
Size: 621 360 3287
Fragments arranged by size:
3287 621 360
RsrII CG'GwC_CG
Cuts at: 2463 2463
Size: 4268
SacI G_AGCT'C
Cuts at: 1282 1282
Size: 4268
SalI G'TCGA_C
Cuts at: 1305 1305
Size: 4268
SapI GCTCTTCn'nnn_
Cuts at: 217 2291 2501 217
Size: 2074 210 1984
Fragments arranged by size:
2074 1984 210
SbfI CC_TGCA'GG
Cuts at: 1315 1315
Size: 4268
ScaI AGT'ACT
Cuts at: 3785 3785
Size: 4268
SfcI C'TryA_G
Cuts at: 33 363 545 1311 1996 2989 3180 33
Size: 330 182 766 685 993 191 1121
Fragments arranged by size:
1121 993 766 685 330 191 182
SfoI GGC'GCC
Cuts at: 472 598 735 988 1700 1948 472
Size: 126 137 253 712 248 2792
Fragments arranged by size:
2792 712 253 248 137 126
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4268
SmaI CCC'GGG
Cuts at: 1174 1290 1174
Size: 116 4152
SmlI C'TyrA_G
Cuts at: 301 1015 1423 2338 2830 3092 301
Size: 714 408 915 492 262 1477
Fragments arranged by size:
1477 915 714 492 408 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4268
SphI G_CATG'C
Cuts at: 231 310 1321 1821 231
Size: 79 1011 500 2678
Fragments arranged by size:
2678 1011 500 79
StyI C'CwwG_G
Cuts at: 107 536 743 905 941 1197 1402 1756 107
Size: 429 207 162 36 256 205 354 2619
Fragments arranged by size:
2619 429 354 256 207 205 162 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 271 468 489 1851 1984 2443 2652 271
Size: 197 21 1362 133 459 209 1887
Fragments arranged by size:
1887 1362 459 209 197 133 21
TatI w'GTAC_w
Cuts at: 3783 3783
Size: 4268
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3785 3817 3785
Size: 32 4236
Tth111I GACn'n_nGTC
Cuts at: 2065 2065
Size: 4268
XbaI T'CTAG_A
Cuts at: 1299 1299
Size: 4268
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 549 1763 549
Size: 1214 3054
XmaI C'CCGG_G
Cuts at: 1172 1288 1172
Size: 116 4152
ZraI GAC'GTC
Cuts at: 713 713
Size: 4268
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AloI AscI AvrII BaeI
BfrBI BglI BmgBI BmtI BsgI BsiWI BsmBI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI